SIST EN 17697:2023
(Main)Animal feeding stuffs - Methods of sampling and analysis - PFGE typing of Lactobacilli, Pediococci, Enterococci and Bacilli in animal feeds
Animal feeding stuffs - Methods of sampling and analysis - PFGE typing of Lactobacilli, Pediococci, Enterococci and Bacilli in animal feeds
This document defines a Pulsed Field Gel Electrophoresis (PFGE) methodology for the identification of authorized probiotic Lactobacillus, Pediococcus, Enterococcus and Bacillus strains. The method can be applied to purified colonies obtained from cultured premixtures and feeds, in order to verify the presence of strains used as feed additives in declared concentrations, even against eventual microbial background resulting from nonsterile matrices.
Futtermittel: Probenahme- und Untersuchungsverfahren - PFGE Typisierung von Laktobazillen, Pediokokken, Enterokokken und Bazillen in Futtermitteln
Dieses Dokument legt eine Methodik der Pulsfeld-Gelelektrophorese (PFGE) zur Identifizierung zugelassener probiotischer Lactobacillus-, Pediococcus-, Enterococcus- und Bacillus-Stämme fest. Das Verfahren kann auf gereinigte Kolonien angewendet werden, die aus kultivierten Vormischungen und Futtermitteln gewonnen werden, um das Vorhandensein von Stämmen, die als Futtermittelzusatzstoffe in deklarierten Konzentrationen verwendet werden, sogar vor einem möglichen mikrobiellen Hintergrund, der aus nicht sterilen Matrices resultiert.
Aliments des animaux : Méthodes d’analyse - Typage EGCP des lactobacilles, pédiocoques, entérocoques et bacilles dans les aliments des animaux
Le présent document définit une technique d’électrophorèse en champ pulsé (EGCP) pour identifier les souches probiotiques autorisées de lactobacilles, pédiocoques, entérocoques et bacilles. La méthode peut être appliquée aux colonies purifiées obtenues à partir de prémélanges et aliments cultivés, pour vérifier la présence de souches utilisées comme additifs alimentaires dans des concentrations déclarées, même contre l’éventuel microbiote annexe résultant de matrices non stériles.
Krma - Metode vzorčenja in analize - Tipizacija laktobacilov, pediokokov, enterokokov in bacilov z metodo PFGE v krmi
Ta dokument določa metodologijo PFGE za identifikacijo odobrenih probiotičnih sevov laktobacilov, pediokokov, enterokokov in bacilov. To metodo je mogoče uporabljati za prečiščene kolonije, pridobljene iz kultiviranih premiksov in krme, da se potrdi prisotnost sevov, ki se uporabljajo kot krmni dodatki v prijavljenih koncentracijah, tudi glede na morebitno mikrobno ozadje, ki je posledica nesterilnih matric.
General Information
Standards Content (Sample)
SLOVENSKI STANDARD
SIST EN 17697:2023
01-september-2023
Krma - Metode vzorčenja in analize - Tipizacija laktobacilov, pediokokov,
enterokokov in bacilov z metodo PFGE v krmi
Animal feeding stuffs - Methods of sampling and analysis - PFGE typing of Lactobacilli,
Pediococci, Enterococci and Bacilli in animal feeds
Futtermittel: Probenahme- und Untersuchungsverfahren - PFGE Typisierung von
Laktobazillen, Pediokokken, Enterokokken und Bazillen in Futtermitteln
Aliments des animaux : Méthodes d’analyse - Typage EGCP des lactobacilles,
pédiocoques, entérocoques et bacilles dans les aliments des animaux
Ta slovenski standard je istoveten z: CEN/TS 17697:2023
ICS:
65.120 Krmila Animal feeding stuffs
SIST EN 17697:2023 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
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SIST EN 17697:2023
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SIST EN 17697:2023
CEN/TS 17697
TECHNICAL SPECIFICATION
SPÉCIFICATION TECHNIQUE
June 2023
TECHNISCHE SPEZIFIKATION
ICS 65.120
English Version
Animal feeding stuffs - Methods of sampling and analysis -
PFGE typing of Lactobacilli, Pediococci, Enterococci and
Bacilli in animal feeds
Aliments des animaux : Méthodes d'analyse - Typage Futtermittel: Probenahme- und
EGCP des lactobacilles, pédiocoques, entérocoques et Untersuchungsverfahren - PFGE Typisierung von
bacilles dans les aliments des animaux Laktobazillen, Pediokokken, Enterokokken und
Bazillen in Futtermitteln
This Technical Specification (CEN/TS) was approved by CEN on 10 March 2023 for provisional application.
The period of validity of this CEN/TS is limited initially to three years. After two years the members of CEN will be requested to
submit their comments, particularly on the question whether the CEN/TS can be converted into a European Standard.
CEN members are required to announce the existence of this CEN/TS in the same way as for an EN and to make the CEN/TS
available promptly at national level in an appropriate form. It is permissible to keep conflicting national standards in force (in
parallel to the CEN/TS) until the final decision about the possible conversion of the CEN/TS into an EN is reached.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2023 CEN All rights of exploitation in any form and by any means reserved Ref. No. CEN/TS 17697:2023 E
worldwide for CEN national Members.
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SIST EN 17697:2023
CEN/TS 17697:2023 (E)
Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative references . 5
3 Terms and definitions . 5
4 Principle . 6
5 Reagents . 6
6 Media . 8
7 Apparatus . 9
8 Procedure . 10
9 Test report .
...
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