ASTM D4783-01

Standard Test Methods for Resistance of Adhesive Preparations in Container to Attack by Bacteria, Yeast, and Fungi

SCOPE
1.1 These test methods cover the determination of the resistance of liquid adhesive preparations to microbial attack in the container by challenging adhesive specimens with cultures of bacteria, yeast, or fungi, and checking for their ability to return to sterility. These test methods return qualitative results.
1.2 The values stated in SI units are to be regarded as the standard. The values in parentheses are for information only.
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. These test methods are designed to be used by persons trained in correct microbiological technique. Specific precautionary statements are given in Section 8.

General Information

• Status: Historical
• Publication Date: 09-Oct-2001
• ICS: 83.180 - Adhesives
• Technical Committee: D14 - Adhesives
• Drafting Committee: D14.30 - Wood Adhesives

Relations

• Replaces: ASTM D4783-98a - Standard Test Methods for Resistance of Adhesive Preparations in Container to Attack by Bacteria, Yeast, and Fungi
• Effective Date: 10-Oct-2001
• Replaced By: ASTM D4783-01e1 - Standard Test Methods for Resistance of Adhesive Preparations in Container to Attack by Bacteria, Yeast, and Fungi
• Effective Date: 10-Oct-2001
• Referred By: ASTM E3152-23 - Standard Guide for Standard Test Methods and Practices Available for Determining Antifungal Activity on Natural or Synthetic Substrates Treated with Antimicrobial Agents
• Effective Date: 10-Oct-2001
• Referred By: ASTM C1894-22 - Standard Guide for Microbially Induced Corrosion of Concrete Products
• Effective Date: 10-Oct-2001
• Referred By: ASTM D8397-23 - Standard Specification for Acrylic and Reactive Adhesives for Installation of Vinyl and Rubber Floor Coverings
• Effective Date: 10-Oct-2001
• Referred By: ASTM D3498-19a - Standard Specification for Adhesives for Field-Gluing Wood Structural Panels (Plywood or Oriented Strand Board) to Wood Based Floor System Framing
• Effective Date: 10-Oct-2001
• Referred By: ASTM D7799-12a(2021) - Standard Specification for Tufted and Woven Broadloom Carpet Adhesives Without Homogenous PVC or Non-PVC Backings
• Effective Date: 10-Oct-2001
• Referred By: ASTM D6465-99(2016) - Standard Guide for Selecting Aerospace and General Purpose Adhesives and Sealants
• Effective Date: 10-Oct-2001

Standards Content (Sample)

NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation:D4783–01
Standard Test Methods for
Resistance of Adhesive Preparations in Container to Attack
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by Bacteria, Yeast, and Fungi
This standard is issued under the fixed designation D 4783; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (e) indicates an editorial change since the last revision or reapproval.
This standard has been approved for use by agencies of the Department of Defense.
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1. Scope* T 487 Fungus Resistance of Paper and Paperboard
2.3 CSMA:
1.1 These test methods cover the determination of the
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Cosmetics Preservation, Method 38
resistanceofliquidadhesivepreparationstomicrobialattackin
the container by challenging adhesive specimens with cultures
3. Terminology
of bacteria, yeast, or fungi, and checking for their ability to
3.1 Definitions—Many terms in these test methods are
return to sterility. These test methods return qualitative results.
defined in Terminology D 907.
1.2 The values stated in SI units are to be regarded as the
3.2 Definitions of Terms Specific to This Standard:
standard. The values in parentheses are for information only.
3.2.1 adhesive preparation—the adhesive as packaged for
1.3 This standard does not purport to address all of the
distribution, storage, and use.
safety concerns, if any, associated with its use. It is the
3.3 Abbreviations:
responsibility of the user of this standard to establish appro-
3.3.1 PBS—phosphate buffered saline.
priate safety and health practices and determine the applica-
3.3.2 PDA—potato dextrose agar.
bility of regulatory limitations prior to use. These test methods
3.3.3 YMPG—yeast malt peptone glucose (agar).
are designed to be used by persons trained in correct micro-
biological technique. Specific precautionary statements are
4. Summary of Test Methods
given in Section 8.
4.1 The adhesive specimen is challenged by inoculation
with a culture of bacteria, yeast, or fungi, which may be a
2. Referenced Documents
2
single species or a mixed culture of several species, following
2.1 ASTM Standards:
the guidelines given in Note 6. The inoculated adhesive
D 907 Terminology of Adhesives
specimenisstoredat21to27°C(70to80°F)for7days,during
D 4299 Test Method for Effect of Bacterial Contamination
which time cultures (streak plates) are made at preset intervals.
on Performance of Adhesive Preparations and Adhesive
3 See Note 2. At any point in the series of challenges, if the
Films
inoculated specimen shows microbial growth on the streak
D 4300 Test Methods for Ability of Adhesive Films to
plates made during the week following the challenge (indicat-
Support or Resist the Growth of Fungi
ing that it has not returned to sterility), the test is discontinued,
E 640 Test Method for Preservatives in Water-Containing
and the sample is reported as not resistant to attack in the
Cosmetics
container by the species or combination of species used as the
NOTE 1—Test Method E 640 is under the jurisdiction of ASTM
inoculum. If the cultures show no growth, the test is repeated
Committee E35 on Pesticides. The procedure in this method outlines a
with up to four challenges. If the specimen tests out as sterile
serial dilution method of determining plate count using a pour plate
following the fourth challenge, it is reported to be resistant to
technique.
attackinthecontainerbythespeciesorcombinationofspecies
2.2 TAPPI Method:
of bacteria, fungi, or yeast used as the inoculum. At the
discretion of the biological laboratory, the test may be discon-
tinued after the second or third challenge. See Section 16 for
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These test methods are under the jurisdiction of ASTM Committee D14 on
further interpretation.
Adhesives and are the direct responsibility of Subcommittee D14.30 on Wood
Adhesives.
4.2 The time necessary to kill is determined by noting the
Current edition approved Oct. 10, 2001. Published December 2001. Originally
earliest streak plate to read sterile. If the 4-h plate is positive
published as D 4783 – 88. Last previous edition D 4783 – 98a.
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or
contact ASTM Customer Service at [e-mail]. For Annual Book of ASTM
Standards volume information, refer to the standard’s Document Summary page on
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the ASTM website. Available from TAPPI, P.O. Box 105113, Atlanta, GA 30348.
3 5
Withdrawn. This method is the same as Test Method E 640.
*A Summary of Changes section appears at the end of this standard.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959, United States.
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D4783–01
and the 24-h plate is negative, the kill time could be narrowed They
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