ASTM D6579-11

Standard Practice for Molecular Weight Averages and Molecular Weight Distribution of Hydrocarbon, Rosin and Terpene Resins by Size-Exclusion Chromatography

SIGNIFICANCE AND USE
The MW averages and the MWD are important characteristics of a resin. They may be used for a variety of correlations for fundamental studies, processing, or product applications. The MW and MWD values may also be used for production quality control of resins.
Limitations—Comparison of SEC molecular weight values should be made only if the data were obtained under identical chromatographic conditions.
SCOPE
1.1 This practice covers the determination of apparent molecular weight (MW) averages and molecular weight distributions (MWD) for THF-soluble hydrocarbon, rosin and terpene resins by size-exclusion chromatography (SEC). This technique is not absolute; it requires calibration with standards of known molecular weight. This practice is applicable to resins containing molecular-weight components that have elution volumes falling within the elution volume range defined by polystyrene standards.
Note 1—SEC is also known as gel permeation chromatography (GPC).
1.2 SEC systems employ low-volume liquid chromatography components and columns packed with relatively small (generally 3 to 20 μm) microporous particles. High-performance liquid chromatography instrumentation and automated data handling systems for data acquisition and processing are also required.
1.3 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

General Information

• Status: Historical
• Publication Date: 31-May-2011
• ICS: 83.080.20 - Thermoplastic materials
• Technical Committee: D01 - Paint and Related Coatings, Materials, and Applications
• Drafting Committee: D01.34 - Pine Chemicals and Hydrocarbon Resins

Relations

• Replaces: ASTM D6579-06 - Standard Practice for Molecular Weight Averages and Molecular Weight Distribution of Hydrocarbon and Terpene Resins by Size-Exclusion Chromatography
• Effective Date: 01-Jun-2011
• Replaced By: ASTM D6579-11(2015) - Standard Practice for Molecular Weight Averages and Molecular Weight Distribution of Hydrocarbon, Rosin and Terpene Resins by Size-Exclusion Chromatography
• Effective Date: 01-Dec-2015
• Referred By: ASTM F2150-19 - Standard Guide for Characterization and Testing of Biomaterial Scaffolds Used in Regenerative Medicine and Tissue-Engineered Medical Products
• Effective Date: 01-Jun-2011

Standards Content (Sample)

NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation: D6579 − 11
StandardPractice for
Molecular Weight Averages and Molecular Weight
Distribution of Hydrocarbon, Rosin and Terpene Resins by
1
Size-Exclusion Chromatography
This standard is issued under the fixed designation D6579; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision.Anumber in parentheses indicates the year of last reapproval.A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope* D3016Practice for Use of Liquid Exclusion Chromatogra-
phy Terms and Relationships
1.1 This practice covers the determination of apparent
D6440Terminology Relating to Hydrocarbon Resins
molecular weight (MW) averages and molecular weight distri-
butions (MWD) for THF-soluble hydrocarbon, rosin and ter-
3. Terminology
pene resins by size-exclusion chromatography (SEC). This
3.1 For definitions of size-exclusion chromatography terms,
technique is not absolute; it requires calibration with standards
see Practice D3016.
of known molecular weight. This practice is applicable to
resins containing molecular-weight components that have elu-
3.2 For definition of terpene resin, see Terminology D804.
tion volumes falling within the elution volume range defined
3.3 For definitions of resin terms, see Terminology D6440.
by polystyrene standards.
4. Summary of Practice
NOTE1—SECisalsoknownasgelpermeationchromatography(GPC).
4.1 Inthispractice,adilutesolutionofahydrocarbon,rosin
1.2 SEC systems employ low-volume liquid chromatogra-
or terpene resin sample is injected into a liquid mobile phase
phy components and columns packed with relatively small
containing the same solvent used to prepare the resin solution.
(generally 3 to 20 µm) microporous particles. High-
The mobile phase transports the resin into and through a
performance liquid chromatography instrumentation and auto-
chromatography column (or set of columns connected in
mated data handling systems for data acquisition and process-
series) packed with a rigid or semirigid, porous substrate that
ing are also required.
separates the molecules according to their size in solution. A
1.3 The values stated in SI units are to be regarded as the
detectormonitorstheeluateasafunctionofelutionvolume(or
standard. The values given in parentheses are for information
time). Upon emerging from the column(s), the fractions of
only.
size-separated molecules are detected and their elution vol-
1.4 This standard does not purport to address all of the
umes (or times) and (usually) concentrations recorded.
safety concerns, if any, associated with its use. It is the
Through calibration, the elution volumes (or times) are con-
responsibility of the user of this standard to establish appro-
verted to apparent molecular weights, and various molecular
priate safety and health practices and determine the applica-
weight parameters for the sample resin are calculated from the
bility of regulatory limitations prior to use.
molecular weight/concentration data.
2. Referenced Documents
5. Significance and Use
2
2.1 ASTM Standards:
5.1 The MW averages and the MWD are important charac-
D804Terminology Relating to Pine Chemicals, Including
teristics of a resin. They may be used for a variety of
Tall Oil and Related Products
correlations for fundamental studies, processing, or product
applications. The MW and MWD values may also be used for
production quality control of resins.
1
This practice is under the jurisdiction of ASTM Committee D01 on Paint and
Related Coatings, Materials, and Applications and is the direct responsibility of
5.2 Limitations—ComparisonofSECmolecularweightval-
Subcommittee D01.34 on Pine Chemicals and Hydrocarbon Resins.
ues should be made only if the data were obtained under
Current edition approved June 1, 2011. Published June 2011. Originally
identical chromatographic conditions.
approved in 2000. Last previous edition approved in 2006 as D6579–06. DOI:
10.1520/D6579-11.
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or 6. Apparatus
contact ASTM Customer Service at [e-mail]. For Annual Book of ASTM
6.1 Solvent Reservoir—The solvent reservoir must hold
Standards volume information, refer to the standard’s Document Summary page on
the ASTM website. sufficient solvent to ensure consistency of composition for a
*A Summary of Changes section appears at the end of this standard
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

---------------------- Page: 1 ----------------------
D6579 − 11
–7 –8
number of analyses. The reservoir should isolate the solvent than10 to10 RIunitandhaveacellvolume≤10µL.Other
from the atmosphere, permit control of the environment in types of detectors may
...

This document is not anASTM standard and is intended only to provide the user of anASTM standard an indication of what changes have been made to the previous version. Because
it may not be technically possible to adequately depict all changes accurately, ASTM recommends that users consult prior editions as appropriate. In all cases only the current version
of the standard as published by ASTM is to be considered the official document.
Designation:D6579–06 Designation:D6579–11
Standard Practice for
Molecular Weight Averages and Molecular Weight
Distribution of Hydrocarbon, Rosin and Terpene Resins by
1
Size-Exclusion Chromatography
This standard is issued under the fixed designation D6579; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope*
1.1This1.1 This practice covers the determination of apparent molecular weight (MW) averages and molecular weight
distributions (MWD) for THF-soluble hydrocarbon, rosin and terpene resins by size-exclusion chromatography (SEC). This
technique is not absolute; it requires calibration with standards of known molecular weight. This practice is applicable to resins
containingmolecular-weightcomponentsthathaveelutionvolumesfallingwithintheelutionvolumerangedefinedbypolystyrene
standards.
NOTE 1—SEC is also known as gel permeation chromatography (GPC).
1.2 SEC systems employ low-volume liquid chromatography components and columns packed with relatively small (generally
3to20µm)microporousparticles.High-performanceliquidchromatographyinstrumentationandautomateddatahandlingsystems
for data acquisition and processing are also required.
1.3 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility
of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory
limitations prior to use.
2. Referenced Documents
2
2.1 ASTM Standards:
D804 Terminology Relating to Naval Stores, Including Tall Oil and Related Products
D3016 Practice for Use of Liquid Exclusion Chromatography Terms and Relationships
D6440 Terminology Relating to Hydrocarbon Resins
3. Terminology
3.1 For definitions of size-exclusion chromatography terms, see Practice D3016.
3.2 For definition of terpene resin, see Terminology D804.
3.3 For definitions of resin terms, see Terminology D6440.
4. Summary of Practice
4.1In4.1 In this practice, a dilute solution of a hydrocarbon, rosin or terpene resin sample is injected into a liquid mobile phase
containing the same solvent used to prepare the resin solution. The mobile phase transports the resin into and through a
chromatography column (or set of columns connected in series) packed with a rigid or semirigid, porous substrate that separates
the molecules according to their size in solution. A detector monitors the eluate as a function of elution volume (or time). Upon
emerging from the column(s), the fractions of size-separated molecules are detected and their elution volumes (or times) and
(usually)concentrationsrecorded.Throughcalibration,theelutionvolumes(ortimes)areconvertedtoapparentmolecularweights,
and various molecular weight parameters for the sample resin are calculated from the molecular weight/concentration data.
5. Significance and Use
5.1 The MW averages and the MWD are important characteristics of a resin. They may be used for a variety of correlations
1
This practice is under the jurisdiction of ASTM Committee D01 on Paint and Related Coatings, Materials, and Applications and is the direct responsibility of
Subcommittee D01.34 on Pine Chemicals and Hydrocarbon Resins.
Current edition approved Nov.June 1, 2006.2011. Published November 2006.June 2011. Originally approved in 2000. Last previous edition approved in 20002006 as
D6579 – 006. DOI: 10.1520/D6579-06.10.1520/D6579-11.
2
For referencedASTM standards, visit theASTM website, www.astm.org, or contactASTM Customer Service at [e-mail]. For AnnualBookofASTM Standards
volume information, refer to the standard’s Document Summary page on the ASTM website.
*A Summary of Changes section appears at the end of this standard.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959, United States.
1

---------------------- Page: 1 ----------------------
D6579–11
for fundamental studies, processing, or product applications. The MW and MWD values may also be used for production quality
control of resins.
5.2 Limitations—Comparison of SEC molecular weight values should be made only if the data were obtained under identical
chromatographic conditions.
6. Appara
...