ASTM E1463-92(2012)
(Guide)Standard Guide for Conducting Static and Flow-Through Acute Toxicity Tests With Mysids From the West Coast of the United States (Withdrawn 2021)
Standard Guide for Conducting Static and Flow-Through Acute Toxicity Tests With Mysids From the West Coast of the United States (Withdrawn 2021)
SIGNIFICANCE AND USE
5.1 Mysids are an important component of both the pelagic and epibenthic community. They are preyed upon by many species of fish, birds, and larger invertebrate species, and they are predators of smaller crustaceans and larval stages of invertebrates. In some cases, they feed upon algae. Mysids are sensitive to both organic and inorganic toxicants (1).3 The ecological importance of mysids, their wide geographical distribution, ability to be cultured in the laboratory, and sensitivity to contaminants make them appropriate acute toxicity test organisms.
5.2 An acute toxicity test is conducted to obtain information concerning the immediate effects of a short-term exposure to a test material on a test organism under specified experimental conditions. An acute toxicity test provides data on the short-term effects that are useful for comparisons to other species but does not provide information on delayed effects.
5.3 Results of acute toxicity tests can be used to predict acute effects likely to occur on aquatic organisms in field conditions except that mysids might avoid exposure when possible.
5.4 Results of acute toxicity tests might be used to compare the acute sensitivities of different species and the acute toxicities of different test materials, and to study the effects of various environmental factors on results of such tests.
5.5 Results of acute toxicity tests might be an important consideration when assessing the hazards of materials to aquatic organisms (see Guide E1023) or when deriving water quality criteria for aquatic organisms (2).
5.6 Results of acute toxicity tests might be useful for studying biological availability of, and structure activity relationships between test materials.
5.7 Results of acute toxicity tests will depend, in part, on the temperature, quality of the food, condition of test organisms, test procedures, and other factors.
SCOPE
1.1 This guide describes procedures for obtaining data concerning the adverse effects of a test material (not food) added to marine and estuarine waters on certain species of marine and estuarine mysids during 96 h of continuous exposure. Juvenile mysids used in these tests are taken from cultures shortly after release from the brood and exposed to varying concentrations of a toxicant in static or flow-through conditions. These procedures will be useful for conducting toxicity tests with other species of mysids, although modifications might be necessary.
1.2 Modifications of these procedures might be justified by special needs or circumstances. Although using appropriate procedures is more important than following prescribed procedures, results of tests conducted using unusual procedures are not likely to be comparable to results of many other tests. Comparisons of results obtained using modified and unmodified versions of these procedures might provide useful information concerning new concepts and procedures for conducting acute tests with other species of mysids.
1.3 The procedures given in this guide are applicable to most chemicals, either individually or in formulations, commercial products, and known or unknown mixtures. With appropriate modifications these procedures can be used to conduct acute tests on factors such as temperature, salinity, and dissolved oxygen. These procedures can also be used to assess the toxicity of potentially toxic discharges such as municipal wastes, oil drilling fluids, produced water from oil well production, and other types of industrial wastes.
1.4 Results of acute toxicity tests with toxicants experimentally added to salt and estuarine waters should usually be reported in terms of a LC50 (median lethal concentration).
1.5 This guide is arranged as follows:
Section
Referenced Documents
2
Terminology
3
Summary of Guide
4
Significance and Use
5
Apparatus
6
Facilities
6.1
Construction Materials ...
General Information
Standards Content (Sample)
NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation: E1463 − 92 (Reapproved 2012)
Standard Guide for
Conducting Static and Flow-Through Acute Toxicity Tests
1
With Mysids From the West Coast of the United States
This standard is issued under the fixed designation E1463; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope 1.5 This guide is arranged as follows:
Section
1.1 This guide describes procedures for obtaining data
concerning the adverse effects of a test material (not food)
Referenced Documents 2
added to marine and estuarine waters on certain species of
Terminology 3
Summary of Guide 4
marine and estuarine mysids during 96 h of continuous
Significance and Use 5
exposure. Juvenile mysids used in these tests are taken from
Apparatus 6
cultures shortly after release from the brood and exposed to Facilities 6.1
Construction Materials 6.2
varying concentrations of a toxicant in static or flow-through
Metering Systems 6.3
conditions. These procedures will be useful for conducting
Test Chambers 6.4
toxicity tests with other species of mysids, although modifica-
Cleaning 6.5
Acceptability 6.6
tions might be necessary.
Safety Precautions 7
Dilution Water 8
1.2 Modifications of these procedures might be justified by
Requirements 8.1
special needs or circumstances. Although using appropriate
Source 8.2
procedures is more important than following prescribed
Treatment 8.3
Characterization 8.4
procedures,resultsoftestsconductedusingunusualprocedures
Test Material 9
are not likely to be comparable to results of many other tests.
General 9.1
Comparisons of results obtained using modified and unmodi-
Stock Solution 9.2
Test Concentrations 9.3
fied versions of these procedures might provide useful infor-
Test Organisms 10
mation concerning new concepts and procedures for conduct-
Species 10.1
ing acute tests with other species of mysids.
Age 10.2
Source 10.3
1.3 The procedures given in this guide are applicable to
Brood Stock 10.4
most chemicals, either individually or in formulations, com- Food 10.5
Handling 10.6
mercial products, and known or unknown mixtures. With
Harvesting Young 10.7
appropriate modifications these procedures can be used to
Quality 10.8
conductacutetestsonfactorssuchastemperature,salinity,and Procedure 11
Experimental Design 11.1
dissolved oxygen. These procedures can also be used to assess
Dissolved Oxygen 11.2
the toxicity of potentially toxic discharges such as municipal
Temperature 11.3
wastes, oil drilling fluids, produced water from oil well Loading 11.4
Salinity 11.5
production, and other types of industrial wastes.
Light 11.6
Beginning of Test 11.7
1.4 Results of acute toxicity tests with toxicants experimen-
Feeding 11.8
tally added to salt and estuarine waters should usually be
Duration of Test 11.9
reported in terms of a LC50 (median lethal concentration).
Biological Data 11.10
Other Measurements 11.11
Analytical Methodology 12
Acceptability of Test 13
1
ThisguideisunderthejurisdictionofASTMCommitteeE50onEnvironmental
Interpretation of Results 14
Assessment, Risk Management and CorrectiveAction and is the direct responsibil-
Report 15
ity of Subcommittee E50.47 on Biological Effects and Environmental Fate.
Appendixes
Current edition approved Dec. 1, 2012. Published January 2013. Originally
Holmesimysis costata X1
approved in 1992. Last previous edition approved in 2004 as E1463 – 92 (2004).
Neomysis mercedis X2
DOI: 10.1520/E1463-92R12.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
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E1463 − 92 (2012)
1.6 The values stated in SI units are to be regarded as the solution that results in the death of 50 % of the test organisms
standard. within the test period (see Definition E943).
1.7 This standard does not purport to address all of the
3.2.2 For definitions of other terms used in this guide, refer
safety concerns, if any, associated with its use. It is the
to Guide E729, Terminology E943, Guides E1191 and E1192,
responsibility of the user of this standard to establish appro-
and Practice E1203. For an explanation of units and symbols,
priate safety and health practices and determine the applica-
refer to IEEE/ASTM SI 10.
bility of regulatory limitations prior to use. Specific precau-
tionary statements are given in Section 7.
4. Summary of Guide
4.1 Thetoxicityofasubstanceinmarineorestuarinewaters
2. Referenced Documents
can be determined through a 96-h acute static test or a
2
2.1 ASTM Standards:
flow-through test. Static tests may be conducted in glass 2-L
E729 Guide for Conducting Acute Toxicity
...
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