ASTM E1044-96(2006)
(Specification)Standard Specification for Glass Serological Pipets (General Purpose and Kahn)
Standard Specification for Glass Serological Pipets (General Purpose and Kahn)
ABSTRACT
This specification covers reusable glass serological pipets used for measuring volumes of liquid. The pipets may be classified into three styles according to operational set-up and should be made with approved glass materials. Each pipet should be straight, of one-piece construction, and properly calibrated. All products should conform to the required dimension of delivery tips, zero gradation line position, dimensions and outflow times, graduation markings, color coding, identification markings, and workmanship.
SCOPE
1.1 This specification covers glass serological pipets, used in measuring volumes of liquids.
General Information
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Standards Content (Sample)
NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
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Designation: E1044 – 96 (Reapproved 2006)
Standard Specification for
Glass Serological Pipets (General Purpose and Kahn)
This standard is issued under the fixed designation E1044; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
This standard has been approved for use by agencies of the Department of Defense.
1. Scope 3.1.4 Capacities (mL)—0.1; 0.1 (Kahn); 0.2; 0.2 (Kahn);
0.25 (Kahn); 0.5; 0.60 (Kahn); 1.0; 2.0; 5.0; 10.0; and 25.0.
1.1 This specification covers glass serological pipets, used
in measuring volumes of liquids.
4. General Requirements
2. Referenced Documents 4.1 Borosilicate Glass—Borosilicate glass for pipets shall
conform to the glass requirements of Type 1, Class A or B of
2.1 ASTM Standards:
Specification E438.
E438 Specification for Glasses in Laboratory Apparatus
4.2 Calibration—Pipets shall be calibrated in accordance
E542 Practice for Calibration of Laboratory Volumetric
with Practice E542 to deliver (TD) the intended capacity of
Apparatus
distilled water at 20°C when the last drop is blown out, except
E671 Specification for Maximum Permissible Thermal Re-
the 0.25 mL Kahn pipet; this Kahn pipet shall have a clear
sidual Stress in Annealed Glass Laboratory Apparatus
ungraduated space of not less than 63 mm between the
E694 Specification for Laboratory Glass Volumetric Appa-
lowermost graduation mark and the tip of the pipet. The pipet
ratus
shall be filled about 10 mm above the capacity line. Remove
E920 Specification for Commercially Packaged Laboratory
any water on the outside of the tip by a downward wipe with
Apparatus
filter paper. Next, place the tip in contact with the wetted wall
E921 Specification for Export Packaged Laboratory Appa-
of a beaker and slowly lower the meniscus to the capacity line.
ratus
Do not remove any water remaining on the tip at this time.
E1133 Practice for Performance Testing of Packaged Labo-
Delivery of the contents into a receiving vessel is made with
ratory Apparatus for United States Government Procure-
the tip in contact with the wall of the vessel. When the water
ments
has ceased to flow, the water remaining in the tip is then blown
E1157 Specification for Sampling and Testing of Reusable
out with one firm puff with the tip in contact with the wall of
Laboratory Glassware
the vessel, if possible. No after-drainage period is allowed.
3. Classification Capacity tolerances shall be within the limits given in Table 1.
4.3 Annealing—The maximum residual thermal stress shall
3.1 Style and Capacity—Pipetscoveredbythisspecification
be such as to conform to Specification E671, except that
shall be of the following styles and capacities:
tempered tips or tempered tops, or both, may be supplied at the
3.1.1 Style I—General purpose top end.
option of the manufacturer. The tempered tip, when examined
3.1.2 Style II—Cotton plugging top end.
in index oil that matches the refractive index of the glass being
3.1.3 Style III—Cotton plugging top end, large tip opening.
used, shall have a temper between 75 and 220 nm.
5. Design
This specification is under the jurisdiction of ASTM Committee E41 on
Laboratory Apparatus and is the direct responsibility of Subcommittee E41.01 on
5.1 Shape—The pipets shall be straight and of one-piece
Apparatus.
construction. Any cross section of a pipet taken in a plane
Current edition approved Nov. 1, 2006. Published December 2006. Originally
perpendicular to the longitudinal axis shall be circular.
approved in 1985. Last previous edition approved in 2001 as E1044 – 96 (2001).
DOI: 10.1520/E1044-96R06.
5.2 Delivery Tips—Delivery tips shall be made with a
For referenced ASTM standards, visit the ASTM website, www.astm.org, or
gradual taper of 15 to 30 mm for capacities up to 5 mL
contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
inclusive, and 20 to 35 mm for 10 and 25 mL capacity pipets.
Standards volume information, refer to the standard’s Document Summary page on
the ASTM website. The end of the tip shall be perpendicular to the longitudinal
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E1044 – 96 (2006)
TABLE 1 Requirements for Glass Serological Pipets
Capacity Graduations, mL Outside
Style I and II: Outflow
Tolerance, Diameter
Time, s
Style III: Nominal Tip Color Coding
Capacity, mL +or–mL Graduated
Opening, mm Band
Main Interval Graduated
Portion, min,
Le
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