ASTM E1044-96(2018)
(Specification)Standard Specification for Glass Serological Pipets (General Purpose and Kahn)
Standard Specification for Glass Serological Pipets (General Purpose and Kahn)
ABSTRACT
This specification covers reusable glass serological pipets used for measuring volumes of liquid. The pipets may be classified into three styles according to operational set-up and should be made with approved glass materials. Each pipet should be straight, of one-piece construction, and properly calibrated. All products should conform to the required dimension of delivery tips, zero gradation line position, dimensions and outflow times, graduation markings, color coding, identification markings, and workmanship.
SCOPE
1.1 This specification covers glass serological pipets, used in measuring volumes of liquids.
1.2 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
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This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the
Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
Designation:E1044 −96 (Reapproved 2018)
Standard Specification for
Glass Serological Pipets (General Purpose and Kahn)
This standard is issued under the fixed designation E1044; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope 3.1.3 Style III—Cotton plugging top end, large tip opening.
3.1.4 Capacities (mL)—0.1; 0.1 (Kahn); 0.2; 0.2 (Kahn);
1.1 This specification covers glass serological pipets, used
0.25 (Kahn); 0.5; 0.60 (Kahn); 1.0; 2.0; 5.0; 10.0; and 25.0.
in measuring volumes of liquids.
1.2 This international standard was developed in accor-
4. General Requirements
dance with internationally recognized principles on standard-
ization established in the Decision on Principles for the
4.1 Borosilicate Glass—Borosilicate glass for pipets shall
Development of International Standards, Guides and Recom-
conform to the glass requirements of Type 1, Class A or B of
mendations issued by the World Trade Organization Technical
Specification E438.
Barriers to Trade (TBT) Committee.
4.2 Calibration—Pipets shall be calibrated in accordance
2. Referenced Documents with Practice E542 to deliver (TD) the intended capacity of
distilled water at 20°C when the last drop is blown out, except
2.1 ASTM Standards:
the 0.25 mL Kahn pipet; this Kahn pipet shall have a clear
E438 Specification for Glasses in Laboratory Apparatus
ungraduated space of not less than 63 mm between the
E542 Practice for Calibration of Laboratory Volumetric
lowermost graduation mark and the tip of the pipet. The pipet
Apparatus
shall be filled about 10 mm above the capacity line. Remove
E671 Specification for Maximum Permissible Thermal Re-
any water on the outside of the tip by a downward wipe with
sidual Stress in Annealed Glass Laboratory Apparatus
filter paper. Next, place the tip in contact with the wetted wall
E694 Specification for Laboratory Glass Volumetric Appa-
of a beaker and slowly lower the meniscus to the capacity line.
ratus
Do not remove any water remaining on the tip at this time.
E920 Specification for Commercially Packaged Laboratory
Delivery of the contents into a receiving vessel is made with
Apparatus
the tip in contact with the wall of the vessel. When the water
E921 Specification for Export Packaged Laboratory Appa-
has ceased to flow, the water remaining in the tip is then blown
ratus
out with one firm puff with the tip in contact with the wall of
E1133 Practice for Performance Testing of Packaged Labo-
the vessel, if possible. No after-drainage period is allowed.
ratory Apparatus for United States Government Procure-
Capacity tolerances shall be within the limits given in Table 1.
ments
E1157 Specification for Sampling and Testing of Reusable
4.3 Annealing—The maximum residual thermal stress shall
Laboratory Glassware
be such as to conform to Specification E671, except that
tempered tips or tempered tops, or both, may be supplied at the
3. Classification
option of the manufacturer. The tempered tip, when examined
3.1 Style and Capacity—Pipets covered by this specification
in index oil that matches the refractive index of the glass being
shall be of the following styles and capacities:
used, shall have a temper between 75 and 220 nm.
3.1.1 Style I—General purpose top end.
3.1.2 Style II—Cotton plugging top end.
5. Design
5.1 Shape—The pipets shall be straight and of one-piece
This specification is under the jurisdiction of ASTM Committee E41 on
construction. Any cross section of a pipet taken in a plane
Laboratory Apparatus and is the direct responsibility of Subcommittee E41.01 on
perpendicular to the longitudinal axis shall be circular.
Laboratory Ware and Supplies.
Current edition approved Jan. 1, 2018. Published January 2018. Originally
5.2 Delivery Tips—Delivery tips shall be made with a
approved in 1985. Last previous edition approved in 2011 as E1044 – 96 (2011).
DOI: 10.1520/E1044-96R18.
gradual taper of 15 to 30 mm for capacities up to 5 mL
For referenced ASTM standards, visit the ASTM website, www.astm.org, or
inclusive, and 20 to 35 mm for 10 and 25 mL capacity pipets.
contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
The end of the tip shall be perpendicular to the longitudinal
Standards volume information, refer to the standard’s Document Summary page on
the ASTM website. axis of the pipet. Sudden constriction at the orifice shall not be
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
E1044−96 (2018)
TABLE 1 Requirements for Glass Serological Pipets
Capacity Graduations, m
...
NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation: E1044 − 96 (Reapproved 2018)
Standard Specification for
Glass Serological Pipets (General Purpose and Kahn)
This standard is issued under the fixed designation E1044; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope 3.1.3 Style III—Cotton plugging top end, large tip opening.
3.1.4 Capacities (mL)—0.1; 0.1 (Kahn); 0.2; 0.2 (Kahn);
1.1 This specification covers glass serological pipets, used
0.25 (Kahn); 0.5; 0.60 (Kahn); 1.0; 2.0; 5.0; 10.0; and 25.0.
in measuring volumes of liquids.
1.2 This international standard was developed in accor-
4. General Requirements
dance with internationally recognized principles on standard-
ization established in the Decision on Principles for the
4.1 Borosilicate Glass—Borosilicate glass for pipets shall
Development of International Standards, Guides and Recom-
conform to the glass requirements of Type 1, Class A or B of
mendations issued by the World Trade Organization Technical
Specification E438.
Barriers to Trade (TBT) Committee.
4.2 Calibration—Pipets shall be calibrated in accordance
2. Referenced Documents
with Practice E542 to deliver (TD) the intended capacity of
distilled water at 20°C when the last drop is blown out, except
2.1 ASTM Standards:
the 0.25 mL Kahn pipet; this Kahn pipet shall have a clear
E438 Specification for Glasses in Laboratory Apparatus
ungraduated space of not less than 63 mm between the
E542 Practice for Calibration of Laboratory Volumetric
lowermost graduation mark and the tip of the pipet. The pipet
Apparatus
shall be filled about 10 mm above the capacity line. Remove
E671 Specification for Maximum Permissible Thermal Re-
any water on the outside of the tip by a downward wipe with
sidual Stress in Annealed Glass Laboratory Apparatus
filter paper. Next, place the tip in contact with the wetted wall
E694 Specification for Laboratory Glass Volumetric Appa-
of a beaker and slowly lower the meniscus to the capacity line.
ratus
Do not remove any water remaining on the tip at this time.
E920 Specification for Commercially Packaged Laboratory
Delivery of the contents into a receiving vessel is made with
Apparatus
the tip in contact with the wall of the vessel. When the water
E921 Specification for Export Packaged Laboratory Appa-
has ceased to flow, the water remaining in the tip is then blown
ratus
out with one firm puff with the tip in contact with the wall of
E1133 Practice for Performance Testing of Packaged Labo-
the vessel, if possible. No after-drainage period is allowed.
ratory Apparatus for United States Government Procure-
Capacity tolerances shall be within the limits given in Table 1.
ments
E1157 Specification for Sampling and Testing of Reusable
4.3 Annealing—The maximum residual thermal stress shall
Laboratory Glassware
be such as to conform to Specification E671, except that
tempered tips or tempered tops, or both, may be supplied at the
3. Classification
option of the manufacturer. The tempered tip, when examined
3.1 Style and Capacity—Pipets covered by this specification
in index oil that matches the refractive index of the glass being
shall be of the following styles and capacities:
used, shall have a temper between 75 and 220 nm.
3.1.1 Style I—General purpose top end.
3.1.2 Style II—Cotton plugging top end.
5. Design
5.1 Shape—The pipets shall be straight and of one-piece
This specification is under the jurisdiction of ASTM Committee E41 on
construction. Any cross section of a pipet taken in a plane
Laboratory Apparatus and is the direct responsibility of Subcommittee E41.01 on
perpendicular to the longitudinal axis shall be circular.
Laboratory Ware and Supplies.
Current edition approved Jan. 1, 2018. Published January 2018. Originally
5.2 Delivery Tips—Delivery tips shall be made with a
approved in 1985. Last previous edition approved in 2011 as E1044 – 96 (2011).
DOI: 10.1520/E1044-96R18.
gradual taper of 15 to 30 mm for capacities up to 5 mL
For referenced ASTM standards, visit the ASTM website, www.astm.org, or
inclusive, and 20 to 35 mm for 10 and 25 mL capacity pipets.
contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
The end of the tip shall be perpendicular to the longitudinal
Standards volume information, refer to the standard’s Document Summary page on
the ASTM website. axis of the pipet. Sudden constriction at the orifice shall not be
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
E1044 − 96 (2018)
TABLE 1 Requirements for Glass Serological Pipets
Capacity Graduations, mL Outside
Style I and II: Outflow
Tolerance, Diameter
Time, s
Style III: Nominal Tip Color Codin
...
This document is not an ASTM standard and is intended only to provide the user of an ASTM standard an indication of what changes have been made to the previous version. Because
it may not be technically possible to adequately depict all changes accurately, ASTM recommends that users consult prior editions as appropriate. In all cases only the current version
of the standard as published by ASTM is to be considered the official document.
Designation: E1044 − 96 (Reapproved 2011) E1044 − 96 (Reapproved 2018)
Standard Specification for
Glass Serological Pipets (General Purpose and Kahn)
This standard is issued under the fixed designation E1044; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope
1.1 This specification covers glass serological pipets, used in measuring volumes of liquids.
1.2 This international standard was developed in accordance with internationally recognized principles on standardization
established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued
by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
2. Referenced Documents
2.1 ASTM Standards:
E438 Specification for Glasses in Laboratory Apparatus
E542 Practice for Calibration of Laboratory Volumetric Apparatus
E671 Specification for Maximum Permissible Thermal Residual Stress in Annealed Glass Laboratory Apparatus
E694 Specification for Laboratory Glass Volumetric Apparatus
E920 Specification for Commercially Packaged Laboratory Apparatus
E921 Specification for Export Packaged Laboratory Apparatus
E1133 Practice for Performance Testing of Packaged Laboratory Apparatus for United States Government Procurements
E1157 Specification for Sampling and Testing of Reusable Laboratory Glassware
3. Classification
3.1 Style and Capacity—Pipets covered by this specification shall be of the following styles and capacities:
3.1.1 Style I—General purpose top end.
3.1.2 Style II—Cotton plugging top end.
3.1.3 Style III—Cotton plugging top end, large tip opening.
3.1.4 Capacities (mL)—0.1; 0.1 (Kahn); 0.2; 0.2 (Kahn); 0.25 (Kahn); 0.5; 0.60 (Kahn); 1.0; 2.0; 5.0; 10.0; and 25.0.
4. General Requirements
4.1 Borosilicate Glass—Borosilicate glass for pipets shall conform to the glass requirements of Type 1, Class A or B of
Specification E438.
4.2 Calibration—Pipets shall be calibrated in accordance with Practice E542 to deliver (TD) the intended capacity of distilled
water at 20°C when the last drop is blown out, except the 0.25 mL Kahn pipet; this Kahn pipet shall have a clear ungraduated space
of not less than 63 mm between the lowermost graduation mark and the tip of the pipet. The pipet shall be filled about 10 mm
above the capacity line. Remove any water on the outside of the tip by a downward wipe with filter paper. Next, place the tip in
contact with the wetted wall of a beaker and slowly lower the meniscus to the capacity line. Do not remove any water remaining
on the tip at this time. Delivery of the contents into a receiving vessel is made with the tip in contact with the wall of the vessel.
When the water has ceased to flow, the water remaining in the tip is then blown out with one firm puff with the tip in contact with
the wall of the vessel, if possible. No after-drainage period is allowed. Capacity tolerances shall be within the limits given in Table
1.
This specification is under the jurisdiction of ASTM Committee E41 on Laboratory Apparatus and is the direct responsibility of Subcommittee E41.01 on
ApparatusLaboratory Ware and Supplies.
Current edition approved Dec. 1, 2011Jan. 1, 2018. Published December 2011January 2018. Originally approved in 1985. Last previous edition approved in 20062011
as E1044 – 96 (2006).(2011). DOI: 10.1520/E1044-96R11.10.1520/E1044-96R18.
For referenced ASTM standards, visit the ASTM website, www.astm.org, or contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM Standards
volume information, refer to the standard’s Document Summary page on the ASTM website.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
E1044 − 96 (2018)
TABLE 1 Requirements for Glass Serological Pipets
Capacity Graduations, mL Outside
Style I and II: Outflow
Tolerance, Diameter
Time, s
Style III: Nominal Tip Color Coding
Capacity, mL + or – mL Graduated
Opening, mm Band
Main Interval Graduated
Portion, min,
Least Value min max
Numbered 0.0 to at least
mm
0.1 0.005 0.01 0.01 0.09 5.5 0.5 3 white
A
0.1 0.005 0.01 0.01 0.09 5.5 0.5 3 2 green
0.2 0.008 0.001 0.02 0.19 5.5 0.5 3 black
A
0.2 0.008 0.001 0.01 0.19 5.5 0.5 3 2 blue
B
0.25 0.008 0.0125 0.05 0.25 5.5 0.5 3 ···
0.5 0.01 0.01 0.05 0.45 5.5 0.5 3 2 yellow
0.5 0.01 0.05 0.05 0.45 5.5 0.5 3 2 black
A
0.60 0.01 0.15 0.15 0.45 6.0 0.5 3 ···
1.0 0.02 0.01 0.1 0.95 6.0 1 5 yellow
1.0 0.02 0.1 0.1 0.9 6.0 1 5 2.0 red
2.0 0.02 0.01 0.02 1.9 6.0 1 5 2.5 2 white
2.0 0.02 0.1 0.2 1.9 6.75 1 5 2.5 green
5.0 0.04 0.
...
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