ASTM E2362-22

Standard Practice for Evaluation of Pre-saturated or Impregnated Towelettes for Hard Surface Disinfection

SIGNIFICANCE AND USE
5.1 This practice may be used to determine if a pre-saturated or impregnated towelette demonstrates antimicrobial effectiveness as a disinfectant on hard surfaces. This practice provides survivor results in the form of a qualitative endpoint (growth positive versus growth negative). The results generated by following this practice do not provide for specific quantitative reductions.
SCOPE
1.1 This practice is designed to evaluate the antimicrobial activity of pre-saturated or impregnated towelettes when used as a hard surface disinfectant.  
1.2 It is the responsibility of the investigator to determine whether Good Laboratory Practices (GLP’s) are required and to follow them when appropriate.  
1.3 This practice should be performed only by those trained in microbiological techniques.  
1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.5 Appropriate modifications to the practice may be required when testing organisms not specified herein.  
1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

General Information

• Status: Published
• Publication Date: 30-Sep-2022
• ICS: 71.100.35 - Chemicals for industrial and domestic disinfection purposes
• Technical Committee: E35 - Pesticides, Antimicrobials, and Alternative Control Agents
• Drafting Committee: E35.15 - Antimicrobial Agents

Standards Content (Sample)

This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the
Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
Designation: E2362 − 22
Standard Practice for
Evaluation of Pre-saturated or Impregnated Towelettes for
1
Hard Surface Disinfection
This standard is issued under the fixed designation E2362; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision.Anumber in parentheses indicates the year of last reapproval.A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope* E2756Terminology Relating toAntimicrobial andAntiviral
Agents
1.1 This practice is designed to evaluate the antimicrobial
2.2 Federal Standard
activity of pre-saturated or impregnated towelettes when used
3
as a hard surface disinfectant. 40 CFR, Part 160Good Laboratory Practice Standards
1.2 It is the responsibility of the investigator to determine
3. Terminology
whether Good Laboratory Practices (GLP’s) are required and
3.1 For definitions of terms used in this practice, refer to
to follow them when appropriate.
Terminology E2756.
1.3 This practice shouldbeperformedonlybythose trained
3.2 Definitions:
in microbiological techniques.
3.2.1 carrier, n—a transportable surface onto which a test
1.4 The values stated in SI units are to be regarded as
organism will be inoculated and dried.
standard. No other units of measurement are included in this
3.2.1.1 Discussion—The carrier will be treated with the test
standard.
substance and subcultured for survivors.
1.5 Appropriate modifications to the practice may be re-
3.2.2 CFU, n—colony forming units
quired when testing organisms not specified herein.
3.2.3 disinfectant, n—a physical or chemical agent or pro-
1.6 This standard does not purport to address all of the
cess that destroys pathogenic or potentially pathogenic micro-
safety concerns, if any, associated with its use. It is the
organisms in/on surfaces or objects.
responsibility of the user of this standard to establish appro-
3.2.4 impregnated, adj—saturated with test substance.
priate safety, health, and environmental practices and deter-
3.2.5 neutralizer, n—a component used to render an active
mine the applicability of regulatory limitations prior to use.
1.7 This international standard was developed in accor- agent incapable of destroying organisms by chemical or
dance with internationally recognized principles on standard- physical means.
ization established in the Decision on Principles for the
3.2.6 pre-saturated, adj—to be filled or impregnated with
Development of International Standards, Guides and Recom-
test substance prior to the time of its intended use.
mendations issued by the World Trade Organization Technical
3.2.7 towelette, n—A paper, cloth or non-woven blend
Barriers to Trade (TBT) Committee.
materialusedasatransporterforacleaningand/ordisinfection
agent.
2. Referenced Documents
2
4
2.1 ASTM Standards:
4. Summary of Practice
D1193Specification for Reagent Water
4.1 A towelette impregnated or pre-saturated with a test
E1054PracticesforEvaluationofInactivatorsofAntimicro-
substance is used to treat a carrier which has been inoculated
bial Agents
withatestorganismafteranaliquotofatestorganismhasbeen
inoculated, evenly distributed to an inoculation area of ap-
1
This practice is under the jurisdiction ofASTM Committee E35 on Pesticides, proximately one square inch (approximately 625 mm), and
Antimicrobials, and Alternative Control Agents and is the direct responsibility of
Subcommittee E35.15 on Antimicrobial Agents.
Current edition approved Oct. 1, 2022 Published November 2022. Originally
3
approved in 2004. Last previous edition approved in 2015 as E2362–15. DOI: Available from the Superintendent of Documents, U.S. Government Printing
10.1520/E2362-22. Office, Washington D.C. 20402
2 4
For referenced ASTM standards, visit the ASTM website, www.astm.org, or UnitedStatesEnvironmentalProtectionAgency,StandardOperatingProcedure
contact ASTM Customer Service at [e-mail]. For Annual Book of ASTM for Disinfectant Towelette Test Against Staphylococcus aureus, Pseudomonas
Standards volume information, refer to the standard’s Document Summary page on aeruginosa, and Salmonella enterica, EPA/OPP Microbiology Laboratory, Ft.
the ASTM website. Meade, MD. SOP# MB09-05, Revised 1/30/13.
*A Summary of Changes section appears at the end of this standard
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

---------------------- Page: 1 ----------------------
E2362 − 22
dried onto the carrier. The carrier is wiped using the pre- 6.19 Pipette—sterile volumetric pipettes.
saturated or impregnated towelette simulating the application
...

This document is not an ASTM standard and is intended only to provide the user of an ASTM standard an indication of what changes have been made to the previous version. Because
it may not be technically possible to adequately depict all changes accurately, ASTM recommends that users consult prior editions as appropriate. In all cases only the current version
of the standard as published by ASTM is to be considered the official document.
Designation: E2362 − 15 E2362 − 22
Standard Practice for
Evaluation of Pre-saturated or Impregnated Towelettes for
1
Hard Surface Disinfection
This standard is issued under the fixed designation E2362; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope Scope*
1.1 This practice is designed to evaluate the antimicrobial activity of pre-saturated or impregnated towelettes when used as a hard
surface disinfectant.
1.2 It is the responsibility of the investigator to determine whether Good Laboratory Practices (GLP’s) are required and to follow
them when appropriate.
1.3 This practice should be performed only by those trained in microbiological techniques.
1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.5 Appropriate modifications to the practice may be required when testing organisms not specified herein.
1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility
of the user of this standard to establish appropriate safety safety, health, and healthenvironmental practices and to determine the
applicability of regulatory limitations prior to use.
1.7 This international standard was developed in accordance with internationally recognized principles on standardization
established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued
by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
2. Referenced Documents
2
2.1 ASTM Standards:
D1193 Specification for Reagent Water
E1054 Practices for Evaluation of Inactivators of Antimicrobial Agents
E2756 Terminology Relating to Antimicrobial and Antiviral Agents
2.2 Federal Standard
3
40 CFR, Part 160 Good Laboratory Practice Standards
1
This practice is under the jurisdiction of ASTM Committee E35 on Pesticides, Antimicrobials, and Alternative Control Agents and is the direct responsibility of
Subcommittee E35.15 on Antimicrobial Agents.
Current edition approved Oct. 1, 2015Oct. 1, 2022 Published October 2015November 2022. Originally approved in 2004. Last previous edition approved in 20092015
as E2362 – 09.E2362 –15. DOI: 10.1520/E2362-15.10.1520/E2362-22.
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or contact ASTM Customer Service at [e-mail]. For Annual Book of ASTM Standards
volume information, refer to the standard’s Document Summary page on the ASTM website.
3
Available from the Superintendent of Documents, U.S. Government Printing Office, Washington D.C. 20402
*A Summary of Changes section appears at the end of this standard
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

---------------------- Page: 1 ----------------------
E2362 − 22
3. Terminology
3.1 carrier, n—a transportable surface onto which a test organism will be inoculated and dried. The carrier will be treated with
the test substance and subcultured for survivors.
3.1 For definitions of terms used in this practice, refer to Terminology E2756.
3.2 CFU, n—colony forming units
3.2 Definitions:
3.2.1 carrier, n—a transportable surface onto which a test organism will be inoculated and dried.
3.2.1.1 Discussion—
The carrier will be treated with the test substance and subcultured for survivors.
3.2.2 CFU, n—colony forming units
3.2.3 disinfectant, n—a physical or chemical agent or process that destroys pathogenic or potentially pathogenic microorganisms
in/on surfaces or objects.
3.2.4 impregnated, adj—saturated with test substance.
3.2.5 neutralizer, n—a component used to render an active agent incapable of destroying organisms by chemical or physical
means.
3.2.6 pre-saturated, adj—to be filled or impregnated with test substance prior to the time of its intended use.
3.2.7 towelette, n—A paper, cloth or non-woven blend material used as a transporter for a cleaning and/or disinfection agent.
3.3 disinfectant, n—a physical or chemical agent or process that destroys pathogenic or potentially pathogenic microorganisms
in/on surfaces or objects.
3.4 impregnated, adj—saturated with test substance.
3.5 neutralizer, n—a component used to render an active agent incapable of destroying organisms by ch
...