Standard Test Method for Resistance of Materials Used in Protective Clothing to Penetration by Blood-Borne Pathogens Using Phi-X174 Bacteriophage Penetration as a Test System

SCOPE
1.1 This test method is used to measure the resistance of materials used in protective clothing to penetration by blood-borne pathogens using a surrogate microbe under conditions of continuous liquid contact. Protective clothing material pass/fail determinations are based on the detection of viral penetration.  
1.1.1 This test method is not always effective in testing protective clothing materials having thick, inner liners which readily absorb the liquid assay fluid.  
1.2 This test method does not apply to all forms or conditions of blood-borne pathogen exposure. Users of the test method should review modes for worker/clothing exposure and assess the appropriateness of this test method for their specific applications.  
1.3 This test method has been specifically defined for modeling the viral penetration of Hepatitis (B and C) and Human Immunodeficiency Viruses transmitted in blood and other potentially infectious body fluids. Inferences for protection from other pathogens must be assessed on a case-by-case basis.  
1.4 This test method addresses only the performance of materials or certain material constructions (for example, seams) used in protective clothing and determined to be viral resistant. This test method does not address the design, overall construction and components, or interfaces of garments or other factors which may affect the overall protection offered by the protective clothing.  
1.5 The values stated in SI units or in other units shall be regarded separately as standard. The values stated in each system must be used independently of the other, without combining values in any way.  
1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

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09-Dec-1997
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ASTM F1671-97b - Standard Test Method for Resistance of Materials Used in Protective Clothing to Penetration by Blood-Borne Pathogens Using Phi-X174 Bacteriophage Penetration as a Test System
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NOTICE: This standard has either been superceded and replaced by a new version or discontinued.
Contact ASTM International (www.astm.org) for the latest information.
Designation: F 1671 – 97b
Standard Test Method for
Resistance of Materials Used in Protective Clothing to
Penetration by Blood-Borne Pathogens Using Phi-X174
1
Bacteriophage Penetration as a Test System
This standard is issued under the fixed designation F 1671; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (e) indicates an editorial change since the last revision or reapproval.
INTRODUCTION
Workers, primarily those in the health care profession, involved in treating and caring for
individuals injured or sick, can be exposed to biological liquids capable of transmitting disease. These
diseases, which may be caused by a variety of microorganisms, can pose significant risks to life and
health. This is especially true of blood-borne viruses which cause Hepatitis [Hepatitis B Virus (HBV)
and Hepatitis C Virus (HCV)] and Acquired Immune Deficiency Syndrome (AIDS) [Human
Immunodeficiency Virus (HIV)]. Since engineering controls can not eliminate all possible exposures,
attention is placed on reducing the potential of direct skin contact through the use of protective
clothing that resists penetration (29 CFR Part 1910.1030). This test method was developed to assess
the effectiveness of materials used in protective clothing for protecting the wearer against contact with
blood-borne pathogens using a surrogate microbe suspended in a body fluid simulant under conditions
of continuous contact.
1. Scope seams) used in protective clothing and determined to be viral
resistant. This test method does not address the design, overall
1.1 This test method is used to measure the resistance of
construction and components, or interfaces of garments or
materials used in protective clothing to penetration by blood-
other factors which may affect the overall protection offered by
borne pathogens using a surrogate microbe under conditions of
the protective clothing.
continuous liquid contact. Protective clothing material pass/fail
1.5 The values stated in SI units or in other units shall be
determinations are based on the detection of viral penetration.
regarded separately as standard. The values stated in each
1.1.1 This test method is not always effective in testing
system must be used independently of the other, without
protective clothing materials having thick, inner liners which
combining values in any way.
readily absorb the liquid assay fluid.
1.6 This standard does not purport to address all of the
1.2 This test method does not apply to all forms or condi-
safety concerns, if any, associated with its use. It is the
tions of blood-borne pathogen exposure. Users of the test
responsibility of the user of this standard to establish appro-
method should review modes for worker/clothing exposure and
priate safety and health practices and determine the applica-
assess the appropriateness of this test method for their specific
bility of regulatory limitations prior to use.
applications.
1.3 This test method has been specifically defined for
2. Referenced Documents
modeling the viral penetration of Hepatitis (B and C) and
2.1 ASTM Standards:
Human Immunodeficiency Viruses transmitted in blood and
D 1331 Test Methods for Surface and Interfacial Tension of
other potentially infectious body fluids. Inferences for protec-
2
Solutions of Surface Active Agents
tion from other pathogens must be assessed on a case-by-case
D 1777 Method for Measuring Thickness of Textile Mate-
basis.
3
rials
1.4 This test method addresses only the performance of
D 3776 Test Method for Mass Per Unit Area (Weight) of
materials or certain material constructions (for example,
4
Fabric
D 3862 Test Method for Retention Characteristics of 0.2 μm
1
This test method is under the jurisdiction of ASTM Committee F-23 on
Protective Clothing and is the direct responsibility of Subcommittee F23.40 on
2
Biological Hazards. Annual Book of ASTM Standards, Vol 15.04.
3
Current edition approved Dec. 10, 1997. Published February 1998. Originally Annual Book of ASTM Standards, Vol 07.01.
4
published as F 1671 – 95 (formerly ES – 22). Last previous edition F 1671 – 97a. Annual Book of ASTM Standards, Vol 07.02.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959, United States.
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NOTICE: This standard has either been superceded and replaced by a new version or discontinued.
Contact ASTM International (www.astm.org) for the latest information.
F 1671 – 97b
Membrane Filters Used in Routine Filtration Procedures 3.1.6.1 Discussion—For the purpose of this test method, the
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for the Evaluation of Microbiological Water Quality primary blood-bor
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