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ASTM E787-81(1996)

(Specification)

Standard Specification for Disposable Glass Micro Blood Collection Pipets

Standard Specification for Disposable Glass Micro Blood Collection Pipets

SCOPE
1.1 This specification covers two dimensionally different disposable glass micropipets used primarily to collect whole human blood specimens for clinical analysis and testing. They are available as coated with heparin or uncoated.

General Information

Status
Historical
Publication Date
26-Feb-1981
Technical Committee
E41 - Laboratory Apparatus
Drafting Committee
E41.01 - Laboratory Ware and Supplies
Current Stage
Ref Project

Relations

Replaced By
ASTM E787-81(2001)e1 - Standard Specification for Disposable Glass Micro Blood Collection Pipets
Effective Date
27-Feb-1981

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ASTM E787-81(1996) - Standard Specification for Disposable Glass Micro Blood Collection PipetsASTM E787-81(1996) - Standard Specification for Disposable Glass Micro Blood Collection Pipets
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ASTM E787-81(1996) - Standard Specification for Disposable Glass Micro Blood Collection Pipets
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NOTICE: This standard has either been superseded and replaced by a new version or discontinued.
Contact ASTM International (www.astm.org) for the latest information.
Designation: E 787 – 81 (Reapproved 1996)
Standard Specification for
Disposable Glass Micro Blood Collection Pipets
This standard is issued under the fixed designation E 787; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (e) indicates an editorial change since the last revision or reapproval.
1. Scope is equal to at least 100 USP units.
1.1 This specification covers two dimensionally different
6. Physical Requirements
disposable glass micropipets used primarily to collect whole
6.1 Design—The disposable glass micro blood collection
human blood specimens for clinical analysis and testing. They
pipets, both short and long, shall be straight and pulled to a
are available as coated with heparin or uncoated.
tapered point at one end. Any cross section of the pipets, taken
2. Referenced Documents in a plane perpendicular to the longitudinal axis, shall be
circular. The pipets shall be lightly firepolished at both ends
2.1 ASTM Standards:
with no run-in and possess color bands to denote presence or
E 438 Specification for Glasses in Laboratory Apparatus
absence of heparin content.
3. Terminology 6.2 Dimensions:
6.2.1 The short Caraway pipet shall be approximately 75
3.1 Definitions of Terms Specific to This Standard:
mm long and 4 mm in outside diameter. The pipet shall hold a
3.1.1 disposable micropipets—in accordance with this
liquid volume of 310 to 470 μL. The tapered point length and
specification and the expected product performance expressed
tip orifice opening shall be as specified in Fig. 1.
in this standard, those pipets which are to be used one time
6.2.2 The long Natelson pipet shall be approximately 150
only. Any institution or individual who reuses a disposable
mm long and 3 mm in outside diameter. The pipet shall hold a
pipet must bear full responsibility for its safety and effective-
liquid volume of 220 to 420 μL. The tapered point length and
ness.
tip orifice opening shall be as specified in Fig. 2.
4. Classification
6.3 Workmanship—The pipets, as illustrated in Fig. 1 and
Fig. 2, shall be free of defects that noticeably detract from their
4.1 This specification covers two dimensionally different
appearance or impair their serviceability. They shall be free of
disposable glass pipets as follows:
lint, or significant foreign matter, loose or embedded when
4.1.1 Short Pipet—Approximately 75 mm long and coated
viewed under normal room lighting. The top and tip ends of the
with heparin (Type I) or uncoated (Type II). These are
pipets shall be cut at approximately 90° to the pipet axis and
commercially recognized as Caraway pipets.
shall not be cracked or have jagged ends or chips that enter the
4.1.2 Long Pipet—Approximately 150 mm long and coated
bore of the pipet.
with heparin (Type I) or uncoated (Type II). These are
6.4 Color Coding—Each disposable glass micro blood col-
commercially recognized as Natelson pipets.
lection pipet shall be color-coded to identify the pipet. The
5. Materials and Manufacture
heparin-coated pipet (Type 1) shall have a red color band. The
uncoated pipet (Type 2) shall have a blue color band. The
5.1 Glass—The pipets shall be fabricated from borosilicate
location of these color bands shall be as specified in Fig. 1 and
glass, Type I, Class B, or soda lime glass, Type II, in
Fig. 2.
accordance with Specification E 438.
6.5 Capillary—The pipets, both short and long, shall be
5.2 Heparin—shall be the ammonium salt isolated from the
capable of drawing sheep plasma or human whole blood the
lungs or intestinal mucosa of beef or pork origin. The heparin
full length of the pipet when tested as specified in 7.1.
potency shall be 1 mg of ammonium heparin compound which
6.6 Fluidity (Type 1, Heparinized, only)—Coagulation of
the sheep plasma or human whole blood shall not be evident
when viewed under normal room lighting and tested as
This specification is under the jurisdiction of ASTM Committee E-41 on
specified in 7.2.
Laboratory Apparatus and is the direct responsibility of Subcommittee E41.01 on
6.7 Lot or Control Number—A lot or control number shall
Apparatus.
Current edition approved Feb. 27, 1981. Published April 1981. be indicated on the intermediate and outer package of pipets.
Annual Book of ASTM Standards, Vol 14.02.
This lot or control number shall be traceable to the origin (raw
Caraway, W. T., and Fanger, H., “Ultramicro Procedures In Clinical Chemis-
try,” American Journal of Clinical Pathology, 25, 1955, pp. 316–331.
Natelson, S., Ph.D., Micro-Techniques of Clinical Chemistry, Charles C.
Thomas, Springfield, Ill., 1961, p. 70. The United States Pharmacopeia, 19th Revision, pp. 229–230.
Copyright © ASTM, 100 Barr Harbor Drive, West Conshohocken, PA 19428-2959, United States.
E 787
7.3.2.1 Positive Control—Fill an uncoated (that is, nonhe-
parinized) pipet with recalcified sheep plasma.
7.3.2.2 Negative Control—Fill a coated (that is, heparin-
ized) pipet with plain sheep plasma.
7.3.3 Procedure—Immediately after the preparation of re-
calcified sheep plasma, fill the pipets by immersing the tips in
the recalcified sheep plasma while holding the pipets near the
horizontal level to facilitate quick filling. Rock the pipet
several times to assure intimate mixing of plasma with heparin
on inner surface of capillary tube. Place the pipets in a
horizontal position. At the end of 1 h, inspect the pipets
containing plasma for evidence of coagulation by carefully
Capacity: 310 to 470 μL
Coding: Red band-heparin-coated (Type I)
scoring and snapping off segments of tubing and placing them
Blue band-uncoated (Type II)
on a flat surface. (Use a black background to facilitate
Dimensions in millimetres
observation and comparison with control sample.) Coagulation
A Overall length 73–77
...

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