ASTM D4198-82(2011)

Standard Test Methods for Evaluating Absorbent Pads Used with Membrane Filters for Bacteriological Analysis and Growth (Withdrawn 2020)

SIGNIFICANCE AND USE
These test methods are appropriate for qualifying absorbent pads used with membrane filters for bacteriological enumeration.
The test methods described are applicable to quality control testing of absorbent pads by the suppliers and users of these pads and to specification testing of absorbent pads intended for use with membrane filters in bacteriological enumeration.
Other pure culture organisms and their appropriate culture medium may be substituted for the E. coli and M-FC media for specification testing, as required.
SCOPE
1.1 These test methods cover the determination of the nutrient-holding capacity and the toxic or nutritive effect on bacterial growth of organisms retained on a membrane filter, when the absorbent pad being tested is used as a nutrient reservoir and medium supply source for the retained bacteria.
1.2 The tests described are conducted on 47-mm diameter disks, although other size disks may be employed for bacterial culture techniques.
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

General Information

• Status: Historical
• Publication Date: 31-May-2011
• ICS: 07.100.10 - Medical microbiology
• Technical Committee: D19 - Water
• Drafting Committee: D19.08 - Membranes and Ion Exchange Materials

Standards Content (Sample)

NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation: D4198 − 82 (Reapproved 2011)
Standard Test Methods for
Evaluating Absorbent Pads Used with Membrane Filters for
1
Bacteriological Analysis and Growth
This standard is issued under the fixed designation D4198; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope 4.2 TestMethodBinvolvesculturingmicro-organismsfrom
suspensions of pure cultures on a 0.45-µm membrane filter,
1.1 These test methods cover the determination of the
which is placed on the test absorbent pad saturated with the
nutrient-holding capacity and the toxic or nutritive effect on
appropriate growth medium. The resultant cultures are com-
bacterial growth of organisms retained on a membrane filter,
pared to cultures grown on spread plates and to membrane
when the absorbent pad being tested is used as a nutrient
filters placed directly on agar with no absorbent pad.
reservoir and medium supply source for the retained bacteria.
1.2 The tests described are conducted on 47-mm diameter
5. Significance and Use
disks, although other size disks may be employed for bacterial
5.1 These test methods are appropriate for qualifying absor-
culture techniques.
bent pads used with membrane filters for bacteriological
1.3 This standard does not purport to address all of the
enumeration.
safety concerns, if any, associated with its use. It is the
5.1.1 The test methods described are applicable to quality
responsibility of the user of this standard to establish appro-
control testing of absorbent pads by the suppliers and users of
priate safety and health practices and determine the applica-
these pads and to specification testing of absorbent pads
bility of regulatory limitations prior to use.
intended for use with membrane filters in bacteriological
enumeration.
2. Referenced Documents
5.2 Other pure culture organisms and their appropriate
2
2.1 ASTM Standards:
culture medium may be substituted for the E. coli and M-FC
D1129 Terminology Relating to Water
media for specification testing, as required.
D1193 Specification for Reagent Water
D3508 Method for Evaluating Water Testing Membrane
3 6. Apparatus
Filters for Fecal Coliform Recovery (Withdrawn 1995)
6.1 Filtration Units for membrane filters with side-arm flask
3. Terminology
and tubing.
3.1 Definitions—For definitions of terms used in these test
6.2 Vacuum Source.
methods, refer to Terminology D1129.
6.3 Vortex Mixer or similar mixer.
6.4 Forceps, flat-bladed.
4. Summary of Test Methods
4.1 Test Method A involves saturating a 47-mm absorbent 6.5 Incubator capable of maintaining temperatures of 44.5
6 0.2°C.
pad with water and determining the volume of water held by
the pad by weighing the pad dry and when fully saturated.
6.6 Stereoscopic Microscope and Illuminator.
6.7 Illuminated Magnifying Stand for counting colonies on
1
agar spread plates.
These methods are under the jurisdiction of ASTM Committee D19 on Water
and are the direct responsibilities of Subcommittee D19.08 on Membranes and Ion
6.8 Hand Tally Counter.
Exchange Materials.
Current edition approved May 1, 2011. Published May 2011. Originally
6.9 Autoclave.
approved in 1982. Last previous edition approved in 2003 as D4198 – 82 (2003).
DOI: 10.1520/D4198-82R11. 6.10 Analytical Balance readable to the nearest 1 mg.
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or
6.11 Petri Dish, 50-mm, nonsterile.
contact ASTM Customer Service at [e-mail]. For Annual Book of ASTM
Standards volume information, refer to the standard’s Document Summary page on
6.12 Expendable Equipment:
the ASTM website.
3
6.12.1 Filters (gridded, 0.45-µm, 47-mm) sterile, for water
The last approved version of this historical standard is referenced on
www.astm.org. testing.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

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D4198 − 82 (2011)
6.12.2 Absorbent pads (47-mm), sterile for the growth test. 10.7 Based on the 24-h plate count, dilute a portion of the
6.12.3 Petri dishes, sterile 50-mm and 100-mm. culture to give a solution with 200 to 800 microorganisms per
6.12.4 Pipets, sterile, 10-mL, 0.1 mL graduations, accuracy millilitre.
of 65%.
6.12.5 Test tubes, sterile, 20-mL, with screw caps.
11. Procedure
6.12.6 Bent glass rod, sterile, for spreading bacterial cul-
11.1 Method A—Water Retention:
tures.
11.1.1 Weigh three dry 50-mm plastic petri dishes to the
6.12.7 Burner, for flame sterilization.
nearest 1 mg, for each lot of pads to be tested.
11.1.2 Randomly select three absorbent pads from each lot,
7. Reage
...