EN 16964:2018

Fertilizers - Extraction of total micro-nutrients in fertilizers using aqua regia

This European Standard specifies a method for the total extraction of boron, cobalt, copper, iron, manganese, molybdenum and zinc with aqua regia from mineral fertilizers containing one or more micro-nutrients.
The extracts can be analysed according to EN 16963, EN 16965, prEN 17041, prEN 17042 and prEN 17043.
This method is also suitable for the extraction of cadmium, chromium, nickel and lead to be determined according to EN 16319; the extraction of mercury to be determined according to EN 16320 and the extraction of arsenic to be determined according to EN 16317.

Düngemittel - Extraktion von Gesamtspurennährstoffen aus Düngemitteln mit Königswasser

Diese Europäische Norm legt ein Verfahren zur vollständigen Extraktion von Bor, Cobalt, Kupfer, Eisen, Mangan, Molybdän und Zink aus mineralischen Düngemitteln, die einen oder mehrere Spurennährstoffe enthalten, mit Königswasser fest.
Die Extrakte können nach EN 16963, EN 16965, prEN 17041, prEN 17042 und prEN 17043 analysiert werden.
Dieses Verfahren ist auch anwendbar für die Extraktion von Cadmium, Chrom, Nickel und Blei, die nach EN 16319 zu bestimmen sind, für die Extraktion von Quecksilber, das nach EN 16320 zu bestimmen ist, und für die Extraktion von Arsen, das nach EN 16317 zu bestimmen ist.

Engrais - Extraction des oligo-éléments totaux des engrais à l'eau régale

La présente norme européenne spécifie la méthode d’extraction totale du bore, du cobalt, du cuivre, du fer, du manganèse, du molybdène et du zinc avec de l’eau régale à partir d’engrais minéraux contenant un ou plusieurs oligo-éléments.
Les extraits peuvent être analysés conformément à l’EN 16963, à l’EN 16965,  au prEN 17041, au prEN 17042 et au prEN 17043.
Cette méthode est également adaptée pour l’extraction du cadmium, du chrome, du nickel et du plomb dosés conformément à l’EN 16319, pour l’extraction du mercure dosé conformément à l’EN 16320 et pour l’extraction de l’arsenic dosé conformément à l’EN 16317.

Gnojila - Ekstrakcija celotnih mikrohranil iz gnojil z zlatotopko

Ta evropski standard določa postopek za popolno ekstrakcijo bora, kobalta, bakra, železa, mangana, molibdena in cinka z zlatotopko iz mineralnih gnojil, ki vsebujejo eno ali več mikrohranil.
Ekstrakti se lahko analizirajo skladno s standardi WI 00260173, prEN 16963, prEN 16965, WI 00260179, WI 00260180 in WI 00260182.
OPOMBA:   Ta metoda je primerna tudi za ekstrakcijo kadmija, kroma, niklja in svinca, ki bo določena skladno s standardom EN 16319, ekstrakcijo živega srebra, ki bo določena skladno s standardom EN 16320, in ekstrakcijo arzena, ki bo določena skladno s standardom EN 16317.

General Information

• Status: Published
• Publication Date: 23-Jan-2018
• Withdrawal Date: 30-Jul-2018
• ICS: 65.080 - Fertilizers
• Technical Committee: CEN/TC 260 - Fertilizers and liming materials
• Drafting Committee: CEN/TC 260/WG 7 - Chemical analysis
• Current Stage: 9093 - Decision to confirm - Review Enquiry
• Start Date: 03-Nov-2023
• Completion Date: 14-Apr-2025
• Directive: 2003/2003 - Regulation (EC) N° 2003/2003 of the European Parlament and of the council of 13 October 2003 relating to fertilisers
• Directive: 2019/1009 - Regulation (EU) 2019/1009 of the European Parliament and of the Council of 5 June 2019 laying down rules on the making available on the market of EU fertilising products and amending Regulations (EC) No 1069/2009 and (EC) No 1107/2009 and repealing Regulation (EC) No 2003/2003
• Mandate: M/335 - Standardization Mandate assigned to the CEN concerning the modernisation of the methods of anylysis of Fertilizers
• Ref Project: SIST EN 16964:2018 - Fertilizers - Extraction of total micro-nutrients in fertilizers using aqua regia

Overview

EN 16964:2018 - "Fertilizers - Extraction of total micro‑nutrients in fertilizers using aqua regia" (CEN) specifies a standardized laboratory procedure for extracting the total content of key micro‑nutrients from mineral fertilizers using aqua regia. The method targets boron, cobalt, copper, iron, manganese, molybdenum and zinc and produces extracts suitable for instrumental analysis (ICP‑AES, FAAS and related methods). The procedure is also applicable for extracting Cd, Cr, Ni and Pb (for EN 16319), Hg (EN 16320) and As (EN 16317).

Key topics and technical requirements

• Principle: extraction of micro‑nutrients by boiling aqua regia under reflux.
• Sample sizes & reagent volumes:
  • For micro‑nutrient content ≤ 10 %: test portion ≈ 3.00 g; add 21 ml HCl (37 %) + 7 ml HNO3 (65 %).
  • For micro‑nutrient content > 10 %: test portion ≈ 1.00 g; same acid volumes with larger final dilution.
  • Alternative: 2.00 g test portion with 14 ml HCl + 4.7 ml HNO3 and 100 ml final volume when 150 ml flasks are unavailable.
• Extraction conditions: allow effervescence to subside, reflux the mixture (condensation zone < 1/3 condenser height) for 2 hours, then cool and dilute to volume (typical final volumetric flasks 150 ml or 500 ml giving 50× or 500× dilution respectively).
• Apparatus & materials:
  • Temperature‑controlled heating with reflux condenser; reaction vessel capacity at least 5× the aqua regia volume.
  • Use non‑borosilicate ware or plastic/silica when determining boron to avoid contamination.
• Reagents & water: analytical grade reagents; water grade 2 per EN ISO 3696.
• Post‑processing: filter/centrifuge if necessary; discard first ≈20 ml of filtrate; prepare blanks; store extracts in tightly closed plastic vessels up to 15 days.
• Safety & competence: procedure requires fume hood, trained staff and normal laboratory safety practices.

Applications and users

• Laboratories performing quality control, regulatory compliance and composition analysis of mineral fertilizers and liming materials.
• Agricultural testing labs and manufacturers needing reliable, comparable data on micro‑nutrient content for product labeling and regulatory submissions.
• Environmental and research labs extracting trace metals from fertilizers for risk assessment or agronomic studies.
• The extracts are compatible with downstream methods such as EN 16963 (ICP‑AES) and EN 16965 (FAAS).

Related standards

• EN 16963 - Determination by ICP‑AES
• EN 16965 - Determination by FAAS
• EN 16319, EN 16320, EN 16317 - aqua regia extraction / determination of Cd, Cr, Ni, Pb, Hg, As
• EN ISO 3696 - Water for analytical laboratory use
• EN 1482‑1 / EN 1482‑2 - Sampling and sample preparation

Keywords: EN 16964:2018, aqua regia extraction, fertilizers, micro‑nutrients, boron, cobalt, copper, iron, manganese, molybdenum, zinc, ICP‑AES, FAAS, fertilizer analysis.

Standards Content (Sample)

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Gnojila - Ekstrakcija celotnih mikrohranil iz gnojil z zlatotopkoDüngemittel - Extraktion von Gesamtspurennährstoffen aus Düngemitteln mit KönigswasserEngrais - Extraction des oligo-éléments totaux des engrais à l'eau régaleFertilizers - Extraction of total micro-nutrients in fertilizers using aqua regia65.080GnojilaFertilizersICS:Ta slovenski standard je istoveten z:EN 16964:2018SIST EN 16964:2018en,fr,de01-junij-2018SIST EN 16964:2018SLOVENSKI
STANDARD
EUROPEAN STANDARD NORME EUROPÉENNE EUROPÄISCHE NORM
EN 16964
January
t r s z ICS
x wä r z r English Version
Fertilizers æ Extraction of total microænutrients in fertilizers using aqua regia Engrais æ Extraction des oligoæéléments totaux des engrais à l 5eau régale
Düngemittel æ Extraktion von Gesamtspurennährstoffen aus Düngemitteln mit Königswasser This European Standard was approved by CEN on
s w October
t r s yä
egulations which stipulate the conditions for giving this European Standard the status of a national standard without any alterationä Upætoædate lists and bibliographical references concerning such national standards may be obtained on application to the CENæCENELEC Management Centre or to any CEN memberä
translation under the responsibility of a CEN member into its own language and notified to the CENæCENELEC Management Centre has the same status as the official versionsä
CEN members are the national standards bodies of Austriaá Belgiumá Bulgariaá Croatiaá Cyprusá Czech Republicá Denmarká Estoniaá Finlandá Former Yugoslav Republic of Macedoniaá Franceá Germanyá Greeceá Hungaryá Icelandá Irelandá Italyá Latviaá Lithuaniaá Luxembourgá Maltaá Netherlandsá Norwayá Polandá Portugalá Romaniaá Serbiaá Slovakiaá Sloveniaá Spainá Swedená Switzerlandá Turkey and United Kingdomä
EUROPEAN COMMITTEE FOR STANDARDIZATION COMITÉ EUROPÉEN DE NORMALISATION EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre:
Rue de la Science 23,
B-1040 Brussels
t r s z CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Membersä Refä Noä EN
s x { x vã t r s z ESIST EN 16964:2018

10 % . 6 8.2.2 Micro-nutrient content > 10 % . 6 8.3 Extraction . 6 9 Precision . 7 10 Test report . 7 Bibliography . 8
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