EN 14152:2014

Foodstuffs - Determination of vitamin B2 by high performance liquid chromatography

This European Standard specifies a method for the determination of vitamin B2 in food by high performance liquid chromatography (HPLC) and fluorescence detection. This method has been validated in two interlaboratory studies. The first study was for the analysis of samples of milk powder and pig's liver ranging from 1,45 mg/100 g to 10,68 mg/100 g. The second study was for the analysis of samples of tube feeding solution, baby food, powdered milk, meal with fruits, yeast, cereal and chocolate powder ranging from 0,21 mg/100 g to 87,1 mg/100 g. Vitamin B2 is the mass fraction of total riboflavin including its phosphorylated derivatives.
For further information on the validation, see Clause 8 and Annex B.

Lebensmittel - Bestimmung von Vitamin B2 mit Hochleistungs-Flüssigchromatographie

Diese Europäische Norm legt ein Verfahren zur Bestimmung von Vitamin B2 in Lebensmitteln mit Hochleistungsflüssigchromatographie (HPLC) und Fluoreszenzdetektion fest. Diese Bestimmung wurde in zwei Ringversuchen validiert. In dem ersten Ringversuch wurde Milchpulver und Schweineleber mit 1,45 mg/100 g bis 10,68 mg/100 g analysiert. Im zweiten Ringversuch wurde Krankenhausnahrung, Babynahrung, Milchpulver, Fertignahrung mit Früchten, Hefe, Getreide und Schokoladenpulver von 0,21 mg/100 g bis 87,1 mg/100 g untersucht. Vitamin B2 ist der Massenanteil an gesamt Riboflavin einschließlich phosphorylierte Derivate.
Weitere Informationen zur Validierung siehe Abschnitt 8 und Anhang B.

Produits alimentaires - Détermination de la teneur en vitamine B2 par chromatographie liquide haute performance

La présente Norme européenne spécifie une méthode de dosage de la vitamine B2 présente dans les produits alimentaires par chromatographie liquide haute performance (CLHP) et par détection fluorimétrique. Cette méthode a été validée lors de deux essais interlaboratoires. Le premier essai concernait l’analyse d’échantillons de lait en poudre et de foie de porc allant de 1,45 mg/100 g à 10,68 mg/100 g. Le deuxième essai s’appliquait à l’analyse d’échantillons de solution pour alimentation entérale, d’aliment pour bébé, de lait en poudre, de substitut de repas aux fruits, de levure, de céréales, de chocolat en poudre allant de 0,21 mg/100 g à 87,1 mg/100 g. La vitamine B2 correspond à la fraction massique de riboflavine totale, y compris ses dérivés phosphorylés.
Pour plus d'informations sur la validation, voir l'Article 8 et l'Annexe B.

Živila - Določevanje vitamina B2 s tekočinsko kromatografijo visoke ločljivosti

Standard EN 14152 določa metodo za določevanje vitamina B2 v živilih s tekočinsko kromatografijo visoke ločljivosti (HPLC) in fluorescentno detekcijo riboflavina v polnozrnati moki, mleku v prahu, mešani zelenjavi, prašičjih jetrih, raztopinah za krmljenje, formulah za dojenčke, grobi moki s sadjem, kvasu, žitaricah in čokoladi v prahu. Ta metoda je bila validirana z dvema medlaboratorijskima študijama. Prva študija je bila namenjena analizi vzorcev mleka v prahu in prašičjih jeter, ki vsebujejo od 1,45 mg/100 g to 10,68 mg/100 g vitamina B2. Druga študija je bila namenjena analizi vzorcev raztopin za krmljenje, formul za dojenčke, mleka v prahu, grobe moke s sadjem, kvasa, žitaric in čokolade v prahu, ki vsebujejo od 0,21 mg/100 g do 87,1 mg/100 g vitamina B2. Vitamin B2 je masni delež celotne vrednosti riboflavina, vključno z njegovimi fosforiliranimi derivati. Za več informacij glede validacije glejte točko 8 in dodatek B.

General Information

• Status: Published
• Publication Date: 03-Jun-2014
• ICS: 67.050 - General methods of tests and analysis for food products
• Technical Committee: CEN/TC 275 - Food analysis - Horizontal methods
• Drafting Committee: CEN/TC 275/WG 9 - Vitamins and Carotenoids
• Current Stage: 9093 - Decision to confirm - Review Enquiry
• Completion Date: 28-Oct-2020
• Ref Project: SIST EN 14152:2014 - Foodstuffs - Determination of vitamin B2 by high performance liquid chromatography

Relations

• Replaces: EN 14152:2003 - Foodstuffs - Determination of vitamin B2 by HPLC
• Effective Date: 11-Jun-2014
• Replaces: EN 14152:2003/AC:2005 - Foodstuffs - Determination of vitamin B2 by HPLC
• Effective Date: 11-Jun-2014

Standards Content (Sample)

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Lebensmittel - Bestimmung von Vitamin B2 mit Hochleistungs-FlüssigchromatographieProduits alimentaires - Détermination de la teneur en vitamine B2 par chromatographie liquide haute performanceFoodstuffs - Determination of vitamin B2 by high performance liquid chromatography67.050Splošne preskusne in analizne metode za živilske proizvodeGeneral methods of tests and analysis for food productsICS:Ta slovenski standard je istoveten z:EN 14152:2014SIST EN 14152:2014en,fr,de01-september-2014SIST EN 14152:2014SLOVENSKI
STANDARDSIST EN 14152:2003/AC:2006SIST EN 14152:20031DGRPHãþD



SIST EN 14152:2014



EUROPEAN STANDARD NORME EUROPÉENNE EUROPÄISCHE NORM
EN 14152
June 2014 ICS 67.050 Supersedes EN 14152:2003English Version
Foodstuffs - Determination of vitamin B2 by high performance liquid chromatography
Produits alimentaires - Détermination de la teneur en vitamine B2 par chromatographie liquide haute performance Lebensmittel - Bestimmung von Vitamin B2 mit Hochleistungs-Flüssigchromatographie This European Standard was approved by CEN on 17 April 2014.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre:
Avenue Marnix 17,
B-1000 Brussels © 2014 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN 14152:2014 ESIST EN 14152:2014



EN 14152:2014 (E) 2 Contents Page Foreword .3 1 Scope .4 2 Normative references .4 3 Principle .4 4 Reagents .4 5 Apparatus .6 6 Procedure .7 7 Calculation .8 8 Precision .9 9 Test report . 10 Annex A (informative)
Examples of HPLC chromatograms . 11 Annex B (informative)
Precision data . 12 Annex C (informative)
Alternatives HPLC systems . 14 Bibliography . 15
SIST EN 14152:2014



EN 14152:2014 (E) 3 Foreword This document (EN 14152:2014) has been prepared by Technical Committee CEN/TC 275 “Food analysis - Horizontal methods”, the secretariat of which is held by DIN. This European Standard sh
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