EN ISO 11348-2:2008
(Main)Water quality - Determination of the inhibitory effect of water samples on the light emission of Vibrio fischeri (Luminescent bacteria test) - Part 2: Method using liquid-dried bacteria (ISO 11348-2:2007)
Water quality - Determination of the inhibitory effect of water samples on the light emission of Vibrio fischeri (Luminescent bacteria test) - Part 2: Method using liquid-dried bacteria (ISO 11348-2:2007)
ISO 11348 describes three methods for determining the inhibition of the luminescence emitted by the marine bacterium Vibrio fischeri (NRRL B‑11177). ISO 11348-2:2007 specifies a method using liquid‑dried bacteria.
This method is applicable to:
waste water;
aqueous extracts and leachates;
fresh water (surface water and ground water);
sea water and brackish water;
eluates of sediment (fresh water, brackish water and sea water);
pore water;
single substances, diluted in water.
Wasserbeschaffenheit - Bestimmung der Hemmwirkung von Wasserproben auf die Lichtemission von Vibrio fischeri (Leuchtbakterientest) - Teil 2: Verfahren mit flüssig getrockneten Bakterien (ISO 11348-2:2007)
Die Reihe von ISO 11348 legt drei Verfahren zur Bestimmung der Hemmung der Lichtemission des marinen Bakteriums Vibrio fischeri (NRRL B 11177) fest. Dieser Teil von ISO 11348 beschreibt ein Verfahren mit flüssig getrockneten Bakterien.
Das Verfahren gilt für:
Abwasser;
wässrige Extrakte und Sickerwasser;
Süßwasser (Oberflächenwasser und Grundwasser);
Meerwasser und Brackwasser;
Eluate von Sedimenten (Süßwasser, Brackwasser und Meerwasser);
Porenwasser;
Einzelstoffe, in Wasser gelöst.
Qualité de l'eau - Détermination de l'effet inhibiteur d'échantillons d'eau sur la luminescence de Vibrio fischeri (Essai de bactéries luminescentes) - Partie 2: Méthode utilisant des bactéries déshydratées (ISO 11348-2:2007)
L'ISO 11348 décrit trois méthodes de détermination de l'inhibition de la luminescence émise par la bactérie marine Vibrio fischeri (NRRL B-11177). L'ISO 11348-2:2007 spécifie une méthode utilisant des bactéries déshydratées.
Cette méthode est applicable aux eaux usées, aux extraits aqueux et aux lixiviats, aux eaux douces (eaux de surface ou souterraines), à l'eau de mer ou aux eaux saumâtres, aux éluats de sédiments (eau douce, eau saumâtre et eau de mer), aux eaux interstitielles et aux substances individuelles diluées dans l'eau.
Kakovost vode - Določevanje zaviralnega učinka vzorcev vode na oddajanje svetlobe Vibrio fischeri (preskus luminiscence bakterije) - 2. del: Metoda z uporabo bakterije, posušene iz tekočine (ISO 11348-2:2007)
Standard ISO 11348 opisuje tri metode za določevanje zaviranja luminiscence, ki jo oddaja morska bakterija Vibrio fischeri (NRRL B-11177). Ta del standarda ISO 11348 določa metodo z uporabo bakterije, posušene iz tekočine. Metoda se uporablja za odpadno vodo, vodne ekstrakte in izcedne vode, sladko vodo (površinsko in podtalno), morsko in slano vodo, izlužke sedimentov (sladka, slana in morska voda), vodo v porah, posamezne snovi, razredčene v vodi.
General Information
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Standards Content (Sample)
SLOVENSKI STANDARD
01-maj-2009
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SIST EN ISO 11348-2:2000
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Water quality - Determination of the inhibitory effect of water samples on the light
emission of Vibrio fischeri (Luminescent bacteria test) - Part 2: Method using liquid-dried
bacteria (ISO 11348-2:2007)
Wasserbeschaffenheit - Bestimmung der Hemmwirkung von Wasserproben auf die
Lichtemission von Vibrio fischeri (Leuchtbakterientest) - Teil 2: Verfahren mit flüssig
getrockneten Bakterien (ISO 11348-2:2007)
Qualité de l'eau - Détermination de l'effet inhibiteur d'échantillons d'eau sur la
luminescence de Vibrio fischeri (Essai de bactéries luminescentes) - Partie 2: Méthode
utilisant des bactéries déshydratées (ISO 11348-2:2007)
Ta slovenski standard je istoveten z: EN ISO 11348-2:2008
ICS:
13.060.70 Preiskava bioloških lastnosti Examination of biological
vode properties of water
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EUROPEAN STANDARD
EN ISO 11348-2
NORME EUROPÉENNE
EUROPÄISCHE NORM
November 2008
ICS 13.060.70 Supersedes EN ISO 11348-2:1998
English Version
Water quality - Determination of the inhibitory effect of water
samples on the light emission of Vibrio fischeri (Luminescent
bacteria test) - Part 2: Method using liquid-dried bacteria (ISO
11348-2:2007)
Qualité de l'eau - Détermination de l'effet inhibiteur Wasserbeschaffenheit - Bestimmung der Hemmwirkung
d'échantillons d'eau sur la luminescence de Vibrio fischeri von Wasserproben auf die Lichtemission von Vibrio fischeri
(Essai de bactéries luminescentes) - Partie 2: Méthode (Leuchtbakterientest) - Teil 2: Verfahren mit flüssig
utilisant des bactéries déshydratées (ISO 11348-2:2007) getrockneten Bakterien (ISO 11348-2:2007)
This European Standard was approved by CEN on 29 October 2008.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as the
official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
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EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36 B-1050 Brussels
© 2008 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 11348-2:2008: E
worldwide for CEN national Members.
Contents Page
Foreword.3
Foreword
The text of ISO 11348-2:2007 has been prepared by Technical Committee ISO/TC 147 “Water quality” of the
International Organization for Standardization (ISO) and has been taken over as EN ISO 11348-2:2008 by
Technical Committee CEN/TC 230 “Water analysis” the secretariat of which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by May 2009, and conflicting national standards shall be withdrawn at the
latest by May 2009.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 11348-2:1998.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Cyprus, Czech
Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia,
Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain,
Sweden, Switzerland and the United Kingdom.
Endorsement notice
The text of ISO 11348-2:2007 has been approved by CEN as a EN ISO 11348-2:2008 without any
modification.
INTERNATIONAL ISO
STANDARD 11348-2
Second edition
2007-12-01
Water quality — Determination
of the inhibitory effect of water samples
on the light emission of Vibrio fischeri
(Luminescent bacteria test) —
Part 2:
Method using liquid-dried bacteria
Qualité de l'eau — Détermination de l'effet inhibiteur d'échantillons
d'eau sur la luminescence de Vibrio fischeri (Essai de bactéries
luminescentes) —
Partie 2: Méthode utilisant des bactéries déshydratées
Reference number
ISO 11348-2:2007(E)
©
ISO 2007
ISO 11348-2:2007(E)
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ii © ISO 2007 – All rights reserved
ISO 11348-2:2007(E)
Contents Page
Foreword. iv
Introduction . v
1 Scope . 1
2 Normative references . 1
3 Principle. 2
4 Interferences . 2
5 Reagents and materials . 2
6 Apparatus . 3
7 Sampling and sample pretreatment. 4
8 Procedure . 4
9 Evaluation. 6
10 Expression of results . 8
11 Criteria of validity. 10
12 Precision. 10
13 Test report . 10
Annex A (informative) Colour-correction method. 11
Annex B (informative) Dilution level D – Preparation of the dilution series. 14
Annex C (informative) Precision data. 17
Annex D (informative) Testing salt water samples with the luminescent bacteria test with
liquid-dried bacteria. 18
Bibliography . 21
ISO 11348-2:2007(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies
(ISO member bodies). The work of preparing International Standards is normally carried out through ISO
technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the
International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International Standards
adopted by the technical committees are circulated to the member bodies for voting. Publication as an
International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights.
ISO 11348-2 was prepared by Technical Committee ISO/TC 147, Water quality, Subcommittee SC 5,
Biological methods.
This second edition cancels and replaces the first edition (ISO 11348-2:1998), which has been technically
revised.
ISO 11348 consists of the following parts, under the general title Water quality — Determination of the
inhibitory effect of water samples on the light emission of Vibrio fischeri (Luminescent bacteria test):
⎯ Part 1: Method using freshly prepared bacteria
⎯ Part 2: Method using liquid-dried bacteria
⎯ Part 3: Method using freeze-dried bacteria
iv © ISO 2007 – All rights reserved
ISO 11348-2:2007(E)
Introduction
The measurements specified in ISO 11348 can be carried out using freshly prepared bacteria, as well as
freeze-dried or liquid-dried bacterial preparations.
Standardized work carried out by DIN Normenausschuss Wasserwesen and ISO/TC 147/SC 5/WG 1 has
shown that, in special cases, these different techniques may deliver different results, especially in the
presence of heavy metals.
Such varying sensitivity is caused by differences in media composition used in the preparation of freeze-dried
or liquid-dried bacteria. These protective media influence the bioavailability of toxicants and/or the light
emission of luminescent bacteria. This means that the origin and type of preparation need to be taken into
account when interpreting the results. This may be difficult sometimes, as freeze-dried and liquid-dried
bacteria may be obtained from different suppliers. This, in turn, can mean that the composition is not known in
detail and therefore cannot be interpreted by the user.
For this reason, in addition to toxicity measurements with freshly prepared bacteria (ISO 11348-1) and
freeze-dried bacteria (ISO 11348-3), a procedure with liquid-dried bacteria is described in this part of
ISO 11348, the performance of which can be interpreted by the user in every detail.
The laboratories responsible for the results have the opportunity to select the most suitable technique based
on expert judgement and information about the water sample to be tested.
INTERNATIONAL STANDARD ISO 11348-2:2007(E)
Water quality — Determination of the inhibitory effect of water
samples on the light emission of Vibrio fischeri (Luminescent
bacteria test) —
...
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