EN ISO 10253:2016
(Main)Water quality - Marine algal growth inhibition test with Skeletonema sp. and Phaeodactylum tricornutum (ISO 10253:2016)
Water quality - Marine algal growth inhibition test with Skeletonema sp. and Phaeodactylum tricornutum (ISO 10253:2016)
ISO 10253:2016 specifies a method for the determination of the inhibition of growth of the unicellular marine algae Skeletonema sp. and Phaeodactylum tricornutum by substances and mixtures contained in sea water or by environmental water samples (effluents, elutriates, etc.).
The method can be used for testing substances that are readily soluble in water and are not significantly degraded or eliminated in any other way from the test medium.
Wasserbeschaffenheit - Wachstumshemmtest mit marinen Algen Skeletonema sp. und Phaeodactylum tricornutum (ISO 10253:2016)
Qualité de l'eau - Essai d'inhibition de la croissance des algues marines avec Skeletonema sp. et Phaeodactylum tricornutum (ISO 10253:2016)
ISO 10253:2016 spécifie une méthode de détermination de l'inhibition de la croissance des algues marines unicellulaires Skeletonema sp. et Phaeodactylum tricornutum provoquée par des substances et des mélanges présents dans l'eau de mer (effluents, élutriats, etc.).
Cette méthode peut être utilisée pour soumettre à l'essai des substances facilement solubles dans l'eau et qui ne sont pas sensiblement dégradées ou éliminées d'autre manière du milieu d'essai.
Kakovost vode - Preskus zaviranja rasti morskih alg s Skeletonema sp. in Phaeodactylum tricornutum (ISO 10253:2016)
Ta dokument določa metodo za določevanje zaviranja rasti enoceličnih morskih alg Skeletonema sp. in Phaeodactylum tricornutum s snovmi in zmesmi, ki jih vsebuje morska voda, ali z vzorci okoljske vode (odplake, elutriati itd.).
To metodo je mogoče uporabiti za preskušanje snovi, ki so zlahka topne v vodi in ne razpadejo občutno ali se izločijo na kakršen koli način iz preskusnega medija.
OPOMBA: s spremembami, navedenimi v standardih ISO 14442 in ISO 5667–16, se lahko uporabi za preskušanje zaviralnih učinkov slabo
topnih in anorganskih snovi, hlapnih spojin, kovinskih spojin, odplak, vzorcev morske vode in elutriatov usedlin.
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
01-junij-2017
1DGRPHãþD
SIST EN ISO 10253:2006
Kakovost vode - Preskus zaviranja rasti morskih alg s Skeletonema sp. in
Phaeodactylum tricornutum (ISO 10253:2016)
Water quality - Marine algal growth inhibition test with Skeletonema sp. and
Phaeodactylum tricornutum (ISO 10253:2016)
Wasserbeschaffenheit - Wachstumshemmtest mit marinen Algen Skeletonema costatum
und Phaeodactylum tricornutum (ISO 10253:2016)
Qualité de l'eau - Essai d'inhibition de la croissance des algues marines avec
Skeletonema sp. et Phaeodactylum tricornutum (ISO 10253:2016)
Ta slovenski standard je istoveten z: EN ISO 10253:2016
ICS:
13.060.70 Preiskava bioloških lastnosti Examination of biological
vode properties of water
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EN ISO 10253
EUROPEAN STANDARD
NORME EUROPÉENNE
November 2016
EUROPÄISCHE NORM
ICS 13.060.70 Supersedes EN ISO 10253:2006
English Version
Water quality - Marine algal growth inhibition test with
Skeletonema sp. and Phaeodactylum tricornutum (ISO
10253:2016)
Qualité de l'eau - Essai d'inhibition de la croissance des Wasserbeschaffenheit - Wachstumshemmtest mit
algues marines avec Skeletonema sp. et Phaeodactylum marinen Algen Skeletonema sp. und Phaeodactylum
tricornutum (ISO 10253:2016) tricornutum (ISO 10253:2016)
This European Standard was approved by CEN on 22 October 2016.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2016 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 10253:2016 E
worldwide for CEN national Members.
Contents Page
European foreword . 3
European foreword
This document (EN ISO 10253:2016) has been prepared by Technical Committee ISO/TC 147 “Water
quality” in collaboration with Technical Committee CEN/TC 230 “Water analysis” the secretariat of
which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by May 2017, and conflicting national standards shall be
withdrawn at the latest by May 2017.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent
rights.
This document supersedes EN ISO 10253:2006.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta,
Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and the United Kingdom.
Endorsement notice
The text of ISO 10253:2016 has been approved by CEN as EN ISO 10253:2016 without any modification.
INTERNATIONAL ISO
STANDARD 10253
Third edition
2016-11-15
Water quality — Marine algal
growth inhibition test with
Skeletonema sp. and Phaeodactylum
tricornutum
Qualité de l’eau — Essai d’inhibition de la croissance des algues
marines avec Skeletonema sp. et Phaeodactylum tricornutum
Reference number
ISO 10253:2016(E)
©
ISO 2016
ISO 10253:2016(E)
© ISO 2016, Published in Switzerland
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form
or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior
written permission. Permission can be requested from either ISO at the address below or ISO’s member body in the country of
the requester.
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Tel. +41 22 749 01 11
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copyright@iso.org
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ii © ISO 2016 – All rights reserved
ISO 10253:2016(E)
Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Materials . 3
5.1 Test organisms . 3
5.2 Reagents. 4
6 Apparatus . 5
7 Procedure. 6
7.1 Preparation of growth medium . 6
7.2 Preparation of pre-culture and inoculum . 6
7.3 Choice of test concentrations . 6
7.4 Preparation of test substance stock solutions . 6
7.5 Preparation of test and control batches . 7
7.6 Incubation . 7
7.7 Measurements . 7
8 Validity criteria . 8
9 Interpretation of data . 8
9.1 Plotting growth curves . 8
9.2 Calculation of percentage inhibition . 8
9.3 Determination of EC(r) .
x 9
10 Expression of results . 9
11 Interpretation of results . 9
12 Test report . 9
Annex A (informative) Preparation of dilution series of mixtures in sea water(effluents
or elutriates) .11
Annex B (informative) Test procedure starting from stored algal inocula, and with direct
measurement of algal growth in spectrophotometric cells .12
Annex C (informative) Performance data .18
Bibliography .19
ISO 10253:2016(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www.iso.org/patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation on the meaning of ISO specific terms and expressions related to conformity assessment,
as well as information about ISO’s adherence to the World Trade Organization (WTO) principles in the
Technical Barriers to Trade (TBT) see the following URL: www.iso.org/iso/foreword.html.
The committee responsible for this document is ISO/TC 147, Water quality, Subcommittee SC 5, Biological
methods.
This third edition cancels and replaces the second edition (ISO 10253:2006), which has been technically
revised.
iv © ISO 2016 – All rights reserved
INTERNATIONAL STANDARD ISO 10253:2016(E)
Water quality — Marine algal growth inhibition test with
Skeletonema sp. and Phaeodactylum tricornutum
WARNING — Persons using this document should be familiar with normal laboratory practice.
This document does not purport to address all of the safety problems, if any, associated with its
use. It is the responsibility of the user to establish appropriate safety and health practices and to
ensure compliance with any national regulatory conditions.
IMPORTANT — It is absolutely essential that tests conducted according to this document be
carried out by suitably trained staff.
1 Scope
This document specifies a method for the determination of the inhibition of growth of the unicellular
marine algae Skeletonema sp. and Phaeodactylum tricornutum by substances and mixtures contained in
sea water or by environmental water samples (effluents, elutriates, etc.).
The method can be used for testing substances that are readily soluble in water and are not significantly
degraded or eliminated in any other way from the test medium.
NOTE With modifications, as described in ISO 14442 and ISO 5667–16, the inhibitory effects of poorly
soluble organic and inorganic materials, volatile compounds, metal compounds, effluents, marine water samples
and elutriates of sediments can be tested.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
ISO 5667-16, Water quality — Sampling — Part 16: Guidance on biotesting of samples
ISO 14442, Water quality — Guidelines for algal growth inhibition tests with poorly soluble materials,
volatile compounds, metals and waste water
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
— IEC Electropedia: available at http://www.electropedia.org/
— ISO Online browsing platform: available at http://www.iso.org/obp
3.1
cell density
number of cells per unit volume of medium
Note 1 to entry: The cell density is expressed as x cells/ml.
ISO 10253:2016(E)
3.2
specific growth rate
µ
proportional rate of increase in cell density per unit of time:
1 dx
μ =× (/1 day)
x dt
where
x is the cell density, expressed in cells per millilitre;
t is the time, expressed in days.
−1
Note 1 to entry: Specific growth rate is expressed in inverse days (day ).
3.3
growth medium
mixture of sea water and nutrients which is used for pre-cultures and controls
3.4
test medium
mixture of sea water, nutrients [growth medium (3.3)] and test material in which algal cells are
incubated
3.5
test batch
mixture of sea water, nutrients and test material [test medium (3.4)] inoculated with algae
3.6
control
mixture of sea water, nutrients [growth medium (3.3)] without test material, inoculated with algae
3.7
effective concentration
EC(r)
x
concentration of test substance which results in an x % reduction in specific growth rate relative to the
controls
4 Principle
Mono-specific algal strains are cultured for several generations in a defined medium containing a
range of concentrations of the test substance, prepared by mixing appropriate quantities of nutrient
concentrate, sea water, stock solutions of the test substance, and an inoculum of exponentially growing
algal cells. The test solutions are incubated for a period of (72 ± 2) h, during which the cell density
in each is measured at intervals of at least every (24 ± 2) h. Inhibition is measured as a reduction in
specific growth rate, relative to control cultures grown under identical conditions.
2 © ISO 2016 – All rights reserved
ISO 10253:2016(E)
5 Materials
5.1 Test organisms
Use either of the following marine algae:
1)
a) Skeletonema sp. (CCAP 1077/1C, NIVA BAC 1); or
b) Phaeodactylum tricornutum Bohlin (CCAP 1052/1A, SAG 1090-1a, NIVA BAC 2).
These algae are important and widely distributed phytoplankton species (phylum Bacillariophyta) in
estuarine and coastal areas.
The recommended algae are available in unialgal, non-axenic cultures from the following sources.
NIVA
Norwegian Institute for Water Research
Gaustadaléen 21
N 0349 Oslo
Norway
CCAP
Dunstaffnage Marine Laboratory
P O Box 3 Oban
Argyll PA37 1QA
United Kingdom
SAG
Collection of Algal Cultures
University of Göttingen
Albrecht-von-Haller Institute for Plant Science
Untere Karspüle 2
37073 Göttingen
Germany
Stock cultures may be maintained in the medium described in 7.1. Regular subculturing is necessary.
Weekly intervals may be necessary for Skeletonema sp., every two or three weeks may be sufficient
for Phaeodactylum tricornutum. The stock cultures may also be maintained for extended periods on
richer algal media such as those recommended by the culture collection. It is recommended to keep the
1) The previous editions of this document suggested the use of two strains of Skeletonema costatum. Following a
taxonomic review of the Skeletonema genus, several strains originally identified as S. costatum may in fact be other
species. In light of this and to enable continuity in the use of previously accepted strains, the present revision of this
document has changed the reference from Skeletonema costatum to Skeletonema sp. to avoid non-compliance for
labs that may be using different strains.
ISO 10253:2016(E)
stock culture in the medium described in 7.1 and in an exponential growth phase immediately before
preparing the pre-culture for testing as described in 7.2.
NOTE Concentrated cultures of the diatom Phaeodactylum tricornutum can also be stored for several
months without losing their viability. Stock cultures for the toxicity tests can easily be prepared from the stored
2)
concentrated cultures .
5.2 Reagents
5.2.1 Water
All water used in the preparation of the synthetic sea water, growth medium and test substance
solutions shall be deionized or of equivalent purity. Take special care to avoid contamination of the
water by inorganic or organic substances during preparation and storage. Equipment made of copper
shall not be used.
5.2.2 Sea water
For culturing and testing Phaeodactylum tricornutum, the growth medium (7.1) is made up by
adding nutrients to either natural [salinity = (30 ± 5) g/kg] or synthetic sea water (approximate
salinity = 33 g/kg). For Skeletonema sp., the use of natural sea water may be necessary for the long-term
maintenance of cultures and may also be necessary for the test medium, because a synthetic sea water
medium may not always support sufficient growth to meet the test quality criteria. If natural sea water
is used, care shall be taken to ensure that it is not polluted.
Prepare synthetic sea water with the composition given in Table 1 (approximate salinity = 33 g/kg). All
the chemicals used shall be of analytical grade.
Table 1 — Synthetic sea water
Concentration of salt in synthetic sea water
Salt
g/l
NaCl 22
MgCl ⋅6H O 9,7
2 2
Na SO (anhydrous) 3,7
2 4
CaCl (anhydrous) 1,0
KCl 0,65
NaHCO 0,20
H BO 0,023
3 3
Filter the sea water (synthetic as well as natural one) through a 0,45 µm membrane filter in order to
remove particulate material and algae.
5.2.3 Nutrients
Prepare three nutrient stock solutions in water, with the compositions given in Table 2.
2) Concentrated Phaeodactylum tricornutum cultures can be supplied by MicroBioTests Inc. Mariakerke-Gent,
Belgium. This information is given for the convenience of users of this document and does not constitute an
endorsement by ISO of this product. Equivalent products may be used if they can be shown to lead to the same
results.
4 © ISO 2016 – All rights reserved
ISO 10253:2016(E)
Table 2 — Nutrient stock solutions
Nutrient Concentration in stock solution Final concentration in test solution
Stock solution 1
FeCl ⋅6H O 48 mg/l 149 µg/l (Fe)
3 2
MnCl ⋅4H O 144 mg/l 605 µg/l (Mn)
2 2
ZnSO ⋅7H O 45 mg/l 150 µg/l (Zn)
4 2
CuSO ⋅5H O 0,157 mg/l 0,6 µg/l (Cu)
4 2
CoCl ⋅6H O 0,404 mg/l 1,5 µg/l (Co)
2 2
H BO 1 140 mg/l 3,0 mg/l (B)
3 3
Na EDTA 1 000 mg/l 15,0 mg/l
Stock solution 2
Thiamin hydrochloride 50 mg/l 25 µg/l
Biotin 0,01 mg/l 0,005 µg/l
Vitamin B (cyanocobalamin) 0,10 mg/l 0,05 µg/l
Stock solution 3
K PO 3,0 g/l 3,0 mg/l; 0,438 mg/l P
3 4
NaNO 50,0 g/l 50,0 mg/l; 8,24 mg/l N
Na SiO ⋅5H O 14,9 g/l 14,9 mg/l; 1,97 mg/l Si
2 3 2
These stock solutions have to be diluted (see 7.1 and Annex A) to obtain the final nutrient concentrations
in the test solutions.
All the chemicals used shall be of reagent grade quality.
Sterilize stock solutions by filtration through a 0,2 µm membrane filter. Stock solutions 1 and 3 may
also be sterilized by autoclaving at 120 °C for at least 15 min.
Store the stock solutions in the dark at 4 °C for a maximum of two months.
6 Apparatus
All equipment which comes into contact with the test medium shall be made of glass or a chemically
inert material.
Use normal laboratory apparatus and in addition the following.
6.1 Temperature-controlled cabinet or room, with a white fluorescent light providing continuous
even illumination, suitable for the lighting requirements specified for the test in 7.6.
6.2 Apparatus for measuring algal cell density, preferably a particle counter or a microscope with a
counting chamber.
Alternatively, determine the state of growth of the algal cultures by an indirect procedure using for
instance a fluorimeter [e.g. in vitro fluorescence (Reference [4])], when sufficiently sensitive and if
shown to be sufficiently well correlated with the cell density. The apparatus used shall be capable of
accurately measuring cell densities as low as the inoculum cell density and to distinguish between algal
growth and disturbing effects, for example, the presence of particulate matter and colour of the sample.
Spectrophotometers may be sufficiently sensitive to measure 10 algal cells/ml providing a sufficient
path length (up to 10 cm) can be used. However, this technique is particularly sensitive to interferences
from suspended material and coloured substances at low cell densities.
Annex B describes a procedure to perform the spectrophotometric measurements of the algal cell
density.
ISO 10253:2016(E)
6.3 Culture flasks, e.g. conical flasks of capacity 250 ml, with air-permeable stoppers.
6.4 Apparatus for membrane filtration, filters of mean pore diameter 0,2 µm and 0,45 µm.
6.5 Autoclave.
6.6 pH-meter.
7 Procedure
7.1 Preparation of growth medium
Add 15 ml of nutrient stock solution 1, 0,5 ml of nutrient stock solution 2 and 1 ml of nutrient stock
solution 3 (see Table 2) to approximately 900 ml of natural or synthetic sea water (5.2.2) and then make
up to 1 l with the same sea water.
Adjust the pH to 8,0 ± 0,2 by adding dilute hydrochloric acid or sodium hydroxide solution.
NOTE Complexing of heavy metals by the relatively high concentration of EDTA present in the nutrient
medium can preclude the testing of effluents containing heavy metals. For guidance, see ISO 14442.
7.2 Preparation of pre-culture and inoculum
A pre-culture shall be started two to four days before the beginning of the test (see Note in 5.1).
Add sufficient cells from the algal stock culture to the growth medium (7.1) to obtain a sufficiently low
3 4
cell density of, e.g. 2 × 10 algal cells/ml to 10 algal cells/ml for three days pre-culturing, in order to
maintain exponential growth until the start of the test. The pre-culture shall be incubated under the
same conditions as those in the test. Measure the cell density in the pre-culture immediately before
use, in order to calculate the required inoculum volume.
7.3 Choice of test concentrations
Algae should be exposed to concentrations of the test substance in a geometric series with a ratio not
exceeding 3,2 (e.g. 1,0 mg/l, 1,8 mg/l, 3,2 mg/l, 5,6 mg/l and 10 mg/l).
The concentrations should be chosen to obtain at least one inhibition below and one inhibition above
the intended EC(r) parameter. Additionally, at least two levels of inhibition between 10 % and 90 %
x
should be included in order to provide data for regression analysis.
NOTE A suitable concentration range is best determined by carrying out a preliminary range-finding test
covering several orders of magnitude of difference between test concentrations. Replication of test concentrations
is not a requirement in the preliminary test.
7.4 Preparation of test substance stock solutions
Prepare stock solutions by dissolving the test substance in growth medium (7.1). Modifications
are necessary when the test substance does not readily dissolve in the test medium, as described in
ISO 14442 and ISO 5667-16.
When testing water samples (effluent, elutriates, etc.), spike them with the nutrient stock solutions
(5.2.3) and, if appropriate, to avoid growth inhibition due to a too low salinity, with sea water salts
(5.2.2) to bring the salinity of the sample up to the salinity of the growth medium. An example of a
dilution scheme for sea water samples is given in Annex A.
Normally, carry out the test without adjusting the pH after addition of the test substance. However,
some substances may exert a toxic effect due to extreme acidity or alkalinity. In order to determine the
toxicity of a substance independent of pH, adjust the pH of the master stock solution (before the dilution
6 © ISO 2016 – All rights reserved
ISO 10253:2016(E)
in series) to 8,0 ± 0,2, using either hydrochloric acid or sodium hydroxide solution. The concentration of
acid
...
Frequently Asked Questions
EN ISO 10253:2016 is a standard published by the European Committee for Standardization (CEN). Its full title is "Water quality - Marine algal growth inhibition test with Skeletonema sp. and Phaeodactylum tricornutum (ISO 10253:2016)". This standard covers: ISO 10253:2016 specifies a method for the determination of the inhibition of growth of the unicellular marine algae Skeletonema sp. and Phaeodactylum tricornutum by substances and mixtures contained in sea water or by environmental water samples (effluents, elutriates, etc.). The method can be used for testing substances that are readily soluble in water and are not significantly degraded or eliminated in any other way from the test medium.
ISO 10253:2016 specifies a method for the determination of the inhibition of growth of the unicellular marine algae Skeletonema sp. and Phaeodactylum tricornutum by substances and mixtures contained in sea water or by environmental water samples (effluents, elutriates, etc.). The method can be used for testing substances that are readily soluble in water and are not significantly degraded or eliminated in any other way from the test medium.
EN ISO 10253:2016 is classified under the following ICS (International Classification for Standards) categories: 13.060.70 - Examination of biological properties of water. The ICS classification helps identify the subject area and facilitates finding related standards.
EN ISO 10253:2016 has the following relationships with other standards: It is inter standard links to EN ISO 10253:2006, EN ISO 10253:2024. Understanding these relationships helps ensure you are using the most current and applicable version of the standard.
You can purchase EN ISO 10253:2016 directly from iTeh Standards. The document is available in PDF format and is delivered instantly after payment. Add the standard to your cart and complete the secure checkout process. iTeh Standards is an authorized distributor of CEN standards.
The EN ISO 10253:2016 standard provides a comprehensive framework for assessing the impact of various substances on marine algal growth, specifically zeroing in on the unicellular species Skeletonema sp. and Phaeodactylum tricornutum. This standard is crucial for professionals involved in marine environmental assessments, offering a robust method for evaluating the potential ecological risks posed by contaminants in marine environments. One of the notable strengths of this standard is its precise focus on substances that are easily soluble in water, which is essential for accurately determining the environmental impact of chemicals found in effluents and other marine water samples. The fact that the standard allows for testing substances not significantly degraded or eliminated from the test medium strengthens its relevance in real-world applications, providing stakeholders with reliable data for regulatory compliance and environmental safety assessments. Moreover, the scope of ISO 10253:2016 is explicitly designed to facilitate thorough investigations into the effects of pollutants on marine algal species. This aligns with increasing global concerns over marine ecosystem health and can aid in the formulation of strategies to protect vital marine habitats from harmful substances. The standard's methodological rigor ensures that laboratories conducting these tests can achieve consistent and repeatable results, thereby enhancing the credibility of environmental assessments across different jurisdictions. Overall, the EN ISO 10253:2016 standard stands out not only for its technical thoroughness but also for its significant contribution to the field of marine environmental science. By providing a standardized approach to studying the inhibition of marine algal growth, it plays a pivotal role in advancing our understanding of chemical impacts on marine ecosystems.
EN ISO 10253:2016は、海水や環境水試料に含まれる物質や混合物が、単細胞の海洋藻類Skeletonema sp.およびPhaeodactylum tricornutumの成長を抑制する方法を定めた重要な規格です。この規格の適用範囲は、これらの藻類が成長する環境下での物質の影響を評価することで、海洋生態系の保護に寄与しています。 この標準化文書の強みは、その詳細な検査手法により、海水中の溶解性物質が藻類に与える影響を明確に定量化できる点です。特に、試験環境において他の方法で重要な物質が分解や除去されない場合に、その物質の影響を確実に評価できることは、研究者や環境科学者にとって非常に有用です。 さらに、EN ISO 10253:2016の関連性は、海洋汚染や水質管理の観点からも見逃せません。この規格が提供する方法論は、海洋環境の保護に向けた課題解決のために不可欠であり、持続可能な水質管理に寄与するものといえます。海藻の成長抑制試験を通じて得られるデータは、政策決定者に対しても価値ある情報を提供し、環境への負荷を軽減するための基盤となります。 以上の点から、EN ISO 10253:2016は、水質試験の領域において重要な役割を果たしており、科学的および環境的な観点から、その標準の採用が推奨されます。
SIST EN ISO 10253:2017 기준서는 해양 알갱이 성장 억제 테스트의 중요한 표준으로, 해양 환경에서의 수질 평가에 기여합니다. 이 문서는 Skeletonema sp.과 Phaeodactylum tricornutum과 같은 단세포 해양 조류의 성장 억제 여부를 평가하는 방법을 명시합니다. 이러한 해양 조류는 수질의 건강성을 가늠하는 중요한 지표이므로, 이 기준서의 중요성이 더욱 부각됩니다. 이 표준의 강점은 해수나 환경 수계에서 발견되는 다양한 물질 또는 혼합물에 대한 검사를 수행할 수 있다는 점입니다. 특히, 물에 잘 녹는 물질에 대한 검사에 적합하며, 환경 샘플(배출수, 용출수 등)에서도 적용 가능한 방법을 제공합니다. 이는 해양 환경 보호 및 수질 관리를 위한 유용한 도구로 작용합니다. 또한, EN ISO 10253:2016 기준서는 해양 생태계의 변화와 오염 물질의 영향을 이해하는 데 필수적인 정보를 제공합니다. 이 표준을 통해 연구자 및 환경 관리자는 해수에서의 물질의 독성을 효과적으로 평가할 수 있으며, 이는 해양 보호 정책 수립에도 기여하게 됩니다. 결론적으로, SIST EN ISO 10253:2017은 해양 조류의 성장 억제를 평가하는 데 필요한 절차와 지침을 제공하여, 해양 생태계 보호를 위한 중요한 역할을 수행하고 있습니다. 이 표준은 해양 환경의 지속 가능성을 증진시키는 데 필수적인 요소로 자리잡고 있습니다.
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