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CEN

EN 17203:2021

(Main)

Foodstuffs - Determination of citrinin in food by HPLC-MS/MS

Foodstuffs - Determination of citrinin in food by HPLC-MS/MS

This document describes a procedure for the determination of the citrinin content in food (cereals, red yeast rice (RYR)), herbs and food supplements by liquid chromatography tandem mass spectrometry (LC-MS/MS).
This method has been validated for citrinin in red yeast rice and in the formulated food supplements in the range of 2,5 μg/kg to 3 000 μg/kg and in wheat flour in the range of 2,5 μg/kg to 100 μg/kg.
Laboratory experiences have shown that this method is also applicable to white rice, herbs such as a powder of ginkgo biloba leaves and the formulated food supplements in the range of 2,5 μg/kg to 50 μg/kg.

Lebensmittel - Bestimmung von Citrinin in Lebensmitteln mit HPLC-MS/MS

Dieses Dokument beschreibt ein Verfahren zur Bestimmung des Citriningehalts in Lebensmitteln (Getreide, rotem Reis [RYR, en: red yeast rice]), Kräutern und Nahrungsergänzungsmitteln mittels Flüssigchromato¬graphie und Tandem Massenspektrometrie (LC MS/MS).
Dieses Verfahren wurde für Citrinin in rotem Reis und in entsprechend zusammengesetzten Nahrungsergänzungsmitteln im Bereich von 2,5 μg/kg bis 3 000 μg/kg sowie für Citrinin in Weizenmehl im Bereich von 2,5 μg/kg bis 100 μg/kg validiert.
Laborerfahrungen haben gezeigt, dass dieses Verfahren auch auf weißen Reis, auf Kräuter wie Ginkgo-Biloba-Blätter in Pulverform und entsprechend zusammengesetzte Nahrungsergänzungsmittel im Bereich von 2,5 μg/kg bis 50 μg/kg anwendbar ist.

Produits alimentaires - Dosage de la citrinine dans les produits alimentaires par CLHP-SM/SM

Le présent document décrit un mode opératoire de détermination de la teneur en citrinine dans les aliments [céréales, levure de riz rouge (LRR)], les herbes aromatiques et les compléments alimentaires par chromatographie liquide couplée à une spectrométrie de masse en tandem (CL-SM/SM).
Cette méthode a été validée pour la citrinine dans la levure de riz rouge et dans les compléments alimentaires à base de levure de riz rouge dans la gamme de concentration de 2,5 μg/kg à 3 000 μg/kg et dans la farine de blé dans la gamme de concentration de 2,5 μg/kg à 100 μg/kg.
Les expériences en laboratoire ont montré que cette méthode est également applicable au riz blanc, aux herbes aromatiques telles que les feuilles de Ginkgo biloba en poudre et aux compléments alimentaires formulés dans la gamme de concentration de 2,5 μg/kg à 50 μg/kg.

Živila - Določevanje citrinina v živilih z metodo HPLC-MS/MS

General Information

Status
Published
Publication Date
04-May-2021
Withdrawal Date
29-Nov-2021
ICS
67.050 - General methods of tests and analysis for food products
Technical Committee
CEN/TC 275 - Food analysis - Horizontal methods
Drafting Committee
CEN/TC 275/WG 5 - Biotoxins
Current Stage
6060 - Definitive text made available (DAV) - Publishing
Start Date
05-May-2021
Due Date
21-Sep-2021
Completion Date
05-May-2021
Directive
2017/625 - Regulation (EU) 2017/625 of the European Parliament and of the Council of 15 March 2017 on official controls and other official activities performed to ensure the application of food and feed law, rules on animal health and welfare, plant health and plant protection products
882/2004 - Regulation (EC) No 882/2004 of the European Parliament and of the Council of 29 April 2004 onofficial controls performed to ensure the verification of compliance with feed and food law, animal health and animal welfare rules
Ref Project
SIST EN 17203:2021 - Foodstuffs - Determination of citrinin in food by HPLC-MS/MS

Relations

Replaces
EN 17203:2018 - Determination of citrinin in food by liquid chromatography tandem mass spectrometry (LC-MS/MS)
Effective Date
08-Jun-2022

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Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN 17203:2021
01-september-2021
Nadomešča:
SIST EN 17203:2019
Živila - Določevanje citrinina v živilih z metodo HPLC-MS/MS
Foodstuffs - Determination of citrinin in food by HPLC-MS/MS
Lebensmittel - Bestimmung von Citrinin in Lebensmitteln mit HPLC-MS/MS
Produits alimentaires - Dosage de la citrinine dans les produits alimentaires par CLHP-
SM/SM
Ta slovenski standard je istoveten z: EN 17203:2021
ICS:
67.050 Splošne preskusne in General methods of tests and
analizne metode za živilske analysis for food products
proizvode
SIST EN 17203:2021 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN 17203:2021

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SIST EN 17203:2021


EN 17203
EUROPEAN STANDARD

NORME EUROPÉENNE

May 2021
EUROPÄISCHE NORM
ICS 67.050 Supersedes EN 17203:2018
English Version

Foodstuffs - Determination of citrinin in food by HPLC-
MS/MS
Produits alimentaires - Dosage de la citrinine dans les Lebensmittel - Bestimmung von Citrinin in
produits alimentaires par CLHP-SM/SM Lebensmitteln mit HPLC-MS/MS
This European Standard was approved by CEN on 19 March 2021.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.





EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2021 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 17203:2021 E
worldwide for CEN national Members.

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SIST EN 17203:2021
EN 17203:2021 (E)
Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative references . 5
3 Terms and definitions . 5
4 Principle . 5
5 Reagents . 5
6 Apparatus and equipment . 8
7 Procedure. 9
7.1 Preparation of the test sample . 9
7.2 Extraction of citrinin . 9
7.3 Spiking procedure . 10
7.4 Preparation of sample test solutions . 10
7.5 LC-MS/MS analysis .
...

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