EN 13098:2019

Workplace exposure - Measurement of airborne microorganisms and microbial compounds - General requirements

This document specifies general requirements for the measurement of microorganisms and microbial compounds.
This document provides also guidelines for the assessment of workplace exposure to airborne microorganisms including the determination of total number and culturable number of microorganisms and microbial compounds in the workplace atmosphere.
This document does not apply to the measurement of viruses.

Exposition am Arbeitsplatz - Messung von luftgetragenen Mikroorganismen und mikrobiellen Bestandteilen - Allgemeine Anforderungen

Dieses Dokument legt allgemeine Anforderungen an die Messung von Mikroorganismen und mikrobiellen Bestandteilen fest.
Dieses Dokument stellt außerdem eine Anleitung für die Beurteilung der Exposition am Arbeitsplatz durch luftgetragene Mikroorganismen, einschließlich der Bestimmung der Gesamtanzahl und der anzüchtbaren Anzahl an Mikroorganismen und mikrobiellen Bestandteilen in der Arbeitsplatzatmosphäre, zur Verfügung.
Dieses Dokument gilt nicht für die Messung von Viren.

Exposition sur les lieux de travail - Mesurage des micro-organismes et des composés microbiens en suspension dans l’air - Exigences générales

Le présent document spécifie les exigences générales relatives au mesurage des micro-organismes et des composés microbiens.
Le présent document fournit également des lignes directrices pour l’évaluation de l’exposition aux micro-organismes en suspension dans l’air sur les lieux de travail, y compris la détermination du nombre total de micro-organismes et du nombre de micro-organismes cultivables et des composés microbiens dans l’atmosphère des lieux de travail.
Le présent document ne s'applique pas au mesurage des virus.

Izpostavljenost na delovnem mestu - Ugotavljanje prisotnosti mikroorganizmov v zraku in merjenje njihovih metabolitov - Splošne zahteve

Ta evropski standard določa splošne zahteve za ugotavljanje prisotnosti mikroorganizmov v zraku in merjenje njihovih metabolitov. Ta evropski standard podaja tudi smernice za oceno izpostavljenosti na delovnem mestu zaradi mikroorganizmov v zraku, vključno z določanjem skupnega števila in števila mikroorganizmov, sposobnih tvorbe kolonij, ter njihovih metabolitov v zraku na delovnem mestu.

General Information

• Status: Published
• Publication Date: 03-Sep-2019
• Withdrawal Date: 30-Mar-2020
• ICS: 07.100.99 - Other standards related to microbiology
• ICS: 13.040.30 - Workplace atmospheres
• Technical Committee: CEN/TC 137 - Assessment of workplace exposure
• Drafting Committee: CEN/TC 137 - Assessment of workplace exposure
• Current Stage: 9020 - Submission to 2 Year Review Enquiry - Review Enquiry
• Start Date: 15-Oct-2024
• Completion Date: 15-Oct-2024
• Ref Project: SIST EN 13098:2019 - Workplace exposure - Measurement of airborne microorganisms and microbial compounds - General requirements

Relations

• Replaces: EN 13098:2000 - Workplace atmosphere - Guidelines for measurement of airborne micro-organisms and endotoxin
• Effective Date: 11-Sep-2019

Standards Content (Sample)

SLOVENSKI STANDARD
01-oktober-2019
Nadomešča:
SIST EN 13098:2003
Izpostavljenost na delovnem mestu - Ugotavljanje prisotnosti mikroorganizmov v
zraku in merjenje njihovih metabolitov - Splošne zahteve
Workplace exposure - Measurement of airborne microorganisms and microbial
compounds - General requirements
Exposition am Arbeitsplatz - Messung von Mikroorganismen und mikrobiellen
Bestandteilen in der Luft - Allgemeine Anforderungen
Exposition sur les lieux de travail - Mesurage de microorganismes et en suspension
dans l'air - Exigences générales
Ta slovenski standard je istoveten z: EN 13098:2019
ICS:
07.100.99 Drugi standardi v zvezi z Other standards related to
mikrobiologijo microbiology
13.040.30 Kakovost zraka na delovnem Workplace atmospheres
mestu
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN 13098
EUROPEAN STANDARD
NORME EUROPÉENNE
September 2019
EUROPÄISCHE NORM
ICS 07.100.99; 13.040.30 Supersedes EN 13098:2000
English Version
Workplace exposure - Measurement of airborne
microorganisms and microbial compounds - General
requirements
Exposition sur les lieux de travail - Mesurage de Exposition am Arbeitsplatz - Messung von
microorganismes et en suspension dans l'air - luftgetragenen Mikroorganismen und mikrobiellen
Exigences générales Bestandteilen - Allgemeine Anforderungen
This European Standard was approved by CEN on 10 June 2019.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2019 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 13098:2019 E
worldwide for CEN national Members.

Contents Page
European foreword . 4
Introduction . 5
1 Scope . 6
2 Normative references . 6
3 Terms and definitions . 6
4 Symbols and abbreviations . 9
5 Measurement of microorganisms and microbial compounds . 9
5.1 Biological agents and biological properties . 9
5.2 Aim of measurement . 10
5.3 Measurement strategy . 10
5.3.1 General . 10
5.3.2 Specification of the objectives for measurement . 10
5.3.3 Specification of the measurement task . 10
5.3.4 Collection of background information . 11
5.3.5 Sampling strategy. 11
5.4 Measurement options . 11
5.5 Uncertainty of measurement . 12
5.6 Variability of exposure level . 12
6 Sampling . 12
6.1 Principles and general requirements . 12
6.2 Sampler . 13
6.2.1 Categories . 13
6.2.2 Requirements . 13
6.3 Pumps . 13
6.4 Operator skills . 13
6.5 Transport and storage of samples . 13
6.5.1 General . 13
6.5.2 Transport . 14
6.5.3 Storage at the laboratory. 14
6.6 Sampling documentation . 14
7 Analytical method . 15
7.1 Requirements . 15
7.2 Validation . 15
7.3 Documentation . 15
7.3.1 General information . 15
7.3.2 Specific information . 16
7.4 Determination of culturable fraction. 17
7.5 Determination of direct cell count by microscopy . 17
7.5.1 General . 17
7.5.2 Epifluorescence microscopy and light microscopy . 17
7.5.3 Scanning electron microscopy . 17
7.6 Determination of microbial compounds . 17
8 Expression of results . 17
8.1 General . 17
8.2 Cultivation methods . 17
8.3 Microscopic methods . 18
8.4 Microbial compounds. 18
9 Test report . 18
Annex A (informative) Recommendations for selection of exposure measuring procedures . 19
Annex B (informative) Sampling form example . 31
Annex C (normative) Determination of airborne microorganisms by cultivation . 33
C.1 General . 33
C.2 Requirements . 33
C.2.1 Suspension media and dilution media . 33
C.2.2 Cultivation media . 33
C.2.3 Cultivation temperature and incubation period . 33
C.2.4 Colony counts . 34
C.2.5 Identification . 34
Annex D (informative) List of generic media . 35
Annex E (informative) Formula and calculation examples for colony counting . 36
E.1 Calculation . 36
E.2 Examples . 37
Bibliography . 39

European foreword
This document (EN 13098:2019) has been prepared by Technical Committee CEN/TC 137 “Assessment
of workplace exposure to chemical and biological agents”, the secretariat of which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by March 2020, and conflicting national standards shall
be withdrawn at the latest by March 2020.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN 13098:2000.
The major technical changes between this European Standard and the previous edition are as follows:
a) document title changed;
b) list of measurable bioaerosol compounds extended;
c) new measuring techniques added;
d) new definitions for “allergen”, “cell count of microorganisms”, “glucan”, “microbial compound”,
“mycotoxin”, and “RFc-recombinant” added;
e) existing definitions technically revised, where necessary;
f) terms and definitions already referred to in EN 1540 deleted;
g) 5.3 on “Measurement strategy” improved by providing more details;
h) Annex A updated with regard to new techniques and methods;
i) Annex B updated with new compounds that can be measured;
j) Annex C updated with regard to new counting strategies and identification methods;
k) new Annex E on “Formula and calculation examples for colony counting” added;
l) Bibliography updated and divided in informative references and other information resources;
m) whole document editorially and technically revised.
According
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