CEN/TS 15606:2009

Foodstuffs - Determination of acesulfame-K, aspartame, neohesperidine-dihydrochalcone and saccharin - High performance liquid chromatographic method

This Technical Specification (prCEN/TS 15606:2009) specifies a high performance liquid chromatographic (HPLC) method for the determination of acesulfame-K, aspartame, neohesperidine-dihydrochalcone and saccharin in foodstuffs. The method has been fully validated [1] for the dialysis procedure through collaborative trial, according to the IUPAC Harmonised Protocol [2], on the following analyte matrix combinations:
   acesulfame-K and aspartame in water-based drink, fruit-based drink, cheesecake with biscuit base, canned soup and instant chocolate drink;
   saccharin in water-based drink, fruit-based drink, cheesecake with biscuit base and canned soup;
   neohesperidine-dihydrochalcone in water-based drink, fruit-based drink and canned soup.

Lebensmittel - Bestimmung von Acesulfam-K, Aspartam, Neohesperidin-Dihydrochalcon und Saccharin - Hochleistungsflüssigchromatographisches Verfahren

Diese Technische Spezifikation (prCEN/TS 15606:2009) legt ein hochleistungs-flüssigchromatographisches (en: high performance liquid chromatographic, (HPLC)) Verfahren zur Bestimmung von Acesulfam-K, Aspartam, Neohesperidin-Dihydrochalcon und Saccharin in Lebensmitteln fest. Das Verfahren ist für das Dialyseverfahren vollständig in einem Ringversuch nach dem IUPAC Harmonized Protocol [2] für die folgenden Analyt/Matrix-Kombinationen validiert worden [1]:
- Acesulfam-K und Aspartam in Getränken auf Wasserbasis, Fruchtsaftgetränken, Käsekuchen mit Biskuit-boden, Dosensuppen und Schokoladengetränkepulver;
- Saccharin in Getränken auf Wasserbasis, Fruchtsaftgetränken, Käsekuchen mit Biskuitboden und Dosen-suppen;
- Neohesperidin-Dihydrochalcon in Getränken auf Wasserbasis, Fruchtsaftgetränken und Dosensuppen.

Produits alimentaires - Dosage de l'acésulfame-K, de l'aspartame, de la saccharine et de la néohespéridine dihydrochalcone - Méthode par chromatographie liquide haute performance

liquide haute performance (CLHP) avec détection UV pour le dosage de l'acésulfame-K, de l'aspartame, de la
néohespéridine dihydrochalcone et de la saccharine dans les produits alimentaires. La méthode a été
entièrement validée [1] pour la technique de dialyse au moyen d'un essai interlaboratoire (voir 8.2, 8.3 et
Annexe C) réalisé, conformément au Protocole Harmonisé [2] de l'UICPA, sur les combinaisons analytematrice
suivantes :
- acésulfame-K (de 86 mg/l à 331 mg/l) et aspartame (de 97 mg/kg à 610 mg/l) dans une boisson à base
d'eau, une boisson à base de fruits, un gâteau au fromage blanc à base de biscuit, un potage en boîte et
une boisson chocolatée instantanée ;
- saccharine (de 70 mg/l à 97 mg/kg) dans une boisson à base d'eau, une boisson à base de fruits, un
gâteau au fromage blanc à base de biscuit et un potage en boîte ;
- néohespéridine dihydrochalcone (de 27 mg/l à 57 mg/kg) dans une boisson à base d'eau, une boisson à
base de fruits et un potage en boîte.

Živila - Določevanje K-acesulfama, aspartama, neohesperidin-dihidrokalcona in saharina - Metoda tekočinske kromatografije visoke ločljivosti

General Information

• Status: Published
• Publication Date: 29-Sep-2009
• ICS: 67.050 - General methods of tests and analysis for food products
• Technical Committee: CEN/TC 275 - Food analysis - Horizontal methods
• Drafting Committee: CEN/TC 275/WG 2 - Sweeteners
• Current Stage: 9093 - Decision to confirm - Review Enquiry
• Start Date: 04-May-2012
• Completion Date: 04-May-2012
• Ref Project: SIST-TS CEN/TS 15606:2009 - Foodstuffs - Determination of acesulfame-K, aspartame, neohesperidine-dihydrochalcone and saccharin - High performance liquid chromatographic method

Standards Content (Sample)

SLOVENSKI STANDARD
SIST-TS CEN/TS 15606:2009
01-december-2009
äLYLOD'RORþHYDQMH.DFHVXOIDPDDVSDUWDPDQHRKHVSHULGLQGLKLGURNDOFRQDLQ
VDKDULQD0HWRGDWHNRþLQVNHNURPDWRJUDILMHYLVRNHORþOMLYRVWL
Foodstuffs - Determination of acesulfame-K, aspartame, neohesperidine-
dihydrochalcone and saccharin - High performance liquid chromatographic method
Lebensmittel - Bestimmung von Acesulfam-K, Aspartam, Neohesperidin-Dihydrochalcon
und Saccharin - Hochleistungs-flüssigchromatographisches Verfahren
Produits alimentaires - Dosage de l'acésulfame-K, de l'aspartame, de la saccharine et de
la néohespéridine dihydrochalcone - Méthode par chromatographie liquide haute
performance
Ta slovenski standard je istoveten z: CEN/TS 15606:2009
ICS:
67.050 Splošne preskusne in General methods of tests and
analizne metode za živilske analysis for food products
proizvode
SIST-TS CEN/TS 15606:2009 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST-TS CEN/TS 15606:2009

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SIST-TS CEN/TS 15606:2009


TECHNICAL SPECIFICATION
CEN/TS 15606

SPÉCIFICATION TECHNIQUE

TECHNISCHE SPEZIFIKATION
September 2009
ICS 67.050
English Version
Foodstuffs - Determination of acesulfame-K, aspartame,
neohesperidine-dihydrochalcone and saccharin - High
performance liquid chromatographic method
Produits alimentaires - Dosage de l'acésulfame-K, de Lebensmittel - Bestimmung von Acesulfam-K, Aspartam,
l'aspartame, de la saccharine et de la néohespéridine Neohesperidin-Dihydrochalcon und Saccharin -
dihydrochalcone - Méthode par chromatographie liquide Hochleistungsflüssigchromatographisches Verfahren
haute performance
This Technical Specification (CEN/TS) was approved by CEN on 20 June 2009 for provisional application.

The period of validity of this CEN/TS is limited initially to three years. After two years the members of CEN will be requested to submit their
comments, particularly on the question whether the CEN/TS can be converted into a European Standard.

CEN members are required to announce the existence of this CEN/TS in the same way as for an EN and to make the CEN/TS available
promptly at national level in an appropriate form. It is permissible to keep conflicting national standards in force (in parallel to the CEN/TS)
until the final decision about the possible conversion of the CEN/TS into an EN is reached.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.






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Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2009 CEN All rights of exploitation in any form and by any means reserved Ref. No. CEN/TS 15606:2009: E
worldwide for CEN national Members.

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SIST-TS CEN/TS 15606:2009
CEN/TS 15606:2009 (E)
Contents Page
Foreword .3
1 Scope .4
2 Normative references .4
3 Principle .4
4 Reagents .4
5 Apparatus and equipment .7
6 Procedure .8
7 Calculations . 11
8 Precision . 12
9 Test report . 14
Annex A (normative) Example for chromatographic conditions which have been proven to lea
...