EN ISO 23783-2:2023
(Main)Automated liquid handling systems - Part 2: Measurement procedures for the determination of volumetric performance (ISO 23783-2:2022)
Automated liquid handling systems - Part 2: Measurement procedures for the determination of volumetric performance (ISO 23783-2:2022)
This document specifies procedures for the determination of volumetric performance of automated liquid handling systems (ALHS), including traceability and estimations of measurement uncertainty of measurement results.
This document is applicable to all ALHS with complete, installed liquid handling devices, including tips and other essential parts needed for delivering a specified volume, which perform liquid handling tasks without human intervention into labware.
NOTE For terminology and general requirements of automated liquid handling systems, see ISO 23783-1. Determination, specification, and reporting of volumetric performance of automated liquid handling systems is described in ISO 23783-3.
Automatisierte Flüssigkeitsdosiersysteme - Teil 2: Messverfahren zur Bestimmung der volumetrischen Leistung (ISO 23783-2:2022)
Dieses Dokument legt Verfahren zur Bestimmung der volumetrischen Leistung von automatisierten Flüssigkeitsdosiersystemen (ALHS), einschließlich Rückverfolgbarkeit und Abschätzung der Messunsicherheit von Messergebnissen fest.
Dieses Dokument ist anwendbar für alle ALHS mit vollständigen, installierten Flüssigkeitsdosiersystemen, einschließlich Spitzen und anderer wesentlicher Teile, die für die Dosierung eines bestimmten Volumens benötigt werden und die Flüssigkeitsdosier-Aufgaben ohne menschliches Zutun in Laborgeräten durchführen.
ANMERKUNG Zur Terminologie und den allgemeinen Anforderungen an automatisierte Flüssigkeitsdosiersysteme siehe ISO 23783 1. Die Bestimmung, Spezifikation und Dokumentation der volumetrischen Leistung von automatisierten Flüssigkeitsdosiersystemen wird in ISO 23783 3 beschrieben.
Systèmes automatisés de manipulation de liquides - Partie 2: Procédures de mesure pour la détermination des performances volumétriques (ISO 23783-2:2022)
Le présent document spécifie les procédures pour la détermination des performances volumétriques des systèmes automatisés de manipulation de liquides (ALHS), y compris la traçabilité et les estimations de l’incertitude de mesure des résultats obtenus.
Le présent document s’applique à tous les ALHS dans lesquels sont installés des dispositifs complets de manipulation de liquides, y compris les cônes et autres composants essentiels nécessaires à la distribution d’un volume spécifié, qui exécutent des tâches de manipulation de liquides avec du matériel de laboratoire, sans intervention humaine.
NOTE Pour la terminologie et les exigences générales applicables aux systèmes automatisés de manipulation de liquides, voir l’ISO 23783-1. La détermination, la spécification et le compte-rendu des performances volumétriques des systèmes automatisés de manipulation de liquides sont décrits dans l’ISO 23783-3.
Avtomatizirani sistemi za ravnanje s tekočinami - 2. del: Merilni postopki za določanje prostorninske zmogljivosti (ISO 23783-2:2022)
Ta dokument določa postopke za določanje volumetrične zmogljivosti avtomatiziranih sistemov za ravnanje s tekočinami (ALHS), vključno s sledljivostjo in ocenami merilne negotovosti merilnih rezultatov.
Ta dokument se uporablja za vse avtomatizirane sisteme za ravnanje s tekočinami s popolnimi, nameščenimi napravami za ravnanje s tekočinami, vključno s konicami in drugimi bistvenimi deli, ki so potrebni za dovajanje določene količine. Gre za sisteme, ki izvajajo opravila ravnanja s tekočinami brez človeškega posredovanja v laboratorijsko opremo.
OPOMBA: Za terminologijo in splošne zahteve avtomatiziranih sistemov za ravnanje s tekočinami glej standard ISO 23783-1. Določanje, specifikacija in poročanje volumetrične zmogljivosti avtomatiziranih sistemov za ravnanje s tekočinami so opisani v standardu ISO 23783-3.
General Information
Standards Content (Sample)
SLOVENSKI STANDARD
01-december-2023
Avtomatizirani sistemi za ravnanje s tekočinami - 2. del: Merilni postopki za
določanje prostorninske zmogljivosti (ISO 23783-2:2022)
Automated liquid handling systems - Part 2: Measurement procedures for the
determination of volumetric performance (ISO 23783-2:2022)
Automatisierte Flüssigkeitsdosiersysteme - Teil 2: Messverfahren zur Bestimmung der
volumetrischen Leistung (ISO 23783-2:2022)
Systèmes automatisés de manipulation de liquides - Partie 2: Procédures de mesure
pour la détermination des performances volumétriques (ISO 23783-2:2022)
Ta slovenski standard je istoveten z: EN ISO 23783-2:2023
ICS:
17.060 Merjenje prostornine, mase, Measurement of volume,
gostote, viskoznosti mass, density, viscosity
71.040.20 Laboratorijska posoda in Laboratory ware and related
aparati apparatus
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EN ISO 23783-2
EUROPEAN STANDARD
NORME EUROPÉENNE
October 2023
EUROPÄISCHE NORM
ICS 17.060; 71.040.20
English Version
Automated liquid handling systems - Part 2: Measurement
procedures for the determination of volumetric
performance (ISO 23783-2:2022)
Systèmes automatisés de manipulation de liquides - Automatisierte Flüssigkeitsdosiersysteme - Teil 2:
Partie 2: Procédures de mesure pour la détermination Messverfahren zur Bestimmung der volumetrischen
des performances volumétriques (ISO 23783-2:2022) Leistung (ISO 23783-2:2022)
This European Standard was approved by CEN on 25 September 2023.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2023 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 23783-2:2023 E
worldwide for CEN national Members.
Contents Page
European foreword . 3
European foreword
The text of ISO 23783-2:2022 has been prepared by Technical Committee ISO/TC 48 "Laboratory
equipment” of the International Organization for Standardization (ISO) and has been taken over as
which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by April 2024, and conflicting national standards shall be
withdrawn at the latest by April 2024.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
Any feedback and questions on this document should be directed to the users’ national standards body.
A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the
United Kingdom.
Endorsement notice
The text of ISO 23783-2:2022 has been approved by CEN as EN ISO 23783-2:2023 without any
modification.
INTERNATIONAL ISO
STANDARD 23783-2
First edition
2022-08
Automated liquid handling systems —
Part 2:
Measurement procedures for
the determination of volumetric
performance
Systèmes automatisés de manipulation de liquides —
Partie 2: Procédures de mesure pour la détermination des
performances volumétriques
Reference number
ISO 23783-2:2022(E)
ISO 23783-2:2022(E)
© ISO 2022
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
or ISO’s member body in the country of the requester.
ISO copyright office
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Phone: +41 22 749 01 11
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland
ii
ISO 23783-2:2022(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Abbreviated terms . 1
5 Measurement methods . 2
5.1 Overview of methods suitable for measuring ALHS performance . 2
5.2 Photometric methods . 9
5.2.1 Dual-dye ratiometric photometric method . 9
5.2.2 Single-dye photometric method . 9
5.2.3 Fluorescence method . 9
5.3 Gravimetric methods . 9
5.3.1 Single channel method . 9
5.3.2 Regression analysis . 10
5.4 Hybrid photometric/gravimetric method . 10
5.5 Dimensional methods . 10
5.5.1 Optical image analysis of droplets . 10
5.5.2 Optical image analysis of capillaries . 11
6 Equipment and preparation.11
6.1 Test equipment . 11
6.2 Manually operated single- and multi-channel pipettes .12
6.3 Preparation for testing .12
7 Thermal expansion .13
8 Traceability and measuring system uncertainty .13
8.1 Traceability . 13
8.2 Estimation of measuring system uncertainty . 13
8.2.1 Whole system approach . 13
8.2.2 Measurement model approach. 13
9 Reporting .14
Annex A (normative) Calculation of liquid volumes from balance readings .15
Annex B (normative) Dual-dye ratiometric photometric procedure .18
Annex C (normative) Single dye photometric procedure .24
Annex D (normative) Gravimetric procedure, single channel measurement .29
Annex E (normative) Gravimetric regression procedure .33
Annex F (normative) Photometric/gravimetric hybrid procedure .39
Annex G (normative) Optical image analysis of droplets .48
Annex H (normative) Fluorescence procedure.57
Annex I (normative) Optical image analysis of capillaries .70
Bibliography .76
iii
ISO 23783-2:2022(E)
Foreword
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electrotechnical standardization.
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described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
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