CEN/TS 17700-3:2022
(Main)Plant biostimulants - Claims - Part 3: Tolerance to abiotic stress resulting from the use of a plant biostimulant
Plant biostimulants - Claims - Part 3: Tolerance to abiotic stress resulting from the use of a plant biostimulant
This document provides guidance for justifying abiotic stress tolerance claim of plant biostimulants used in agriculture.
This document is aimed primarily at manufacturers, laboratories, companies which will put the products on the market, notifying authorities, notified bodies, and market surveillance authorities.
Pflanzen-Biostimulanzien - Auslobungen - Teil 3: Toleranz gegenüber abiotischem Stress infolge der Verwendung eines Pflanzen-Biostimulans
Dieses Dokument dient als Leitfaden für die Begründung von Auslobungen zur Toleranz gegenüber abiotischem Stress von Pflanzen-Biostimulanzien in der Landwirtschaft.
Dieses Dokument richtet sich in erster Linie an Hersteller, Labore, Forschungstätige, technische Zentren, Unternehmen, die die Produkte in Verkehr bringen werden, notifizierende Behörden, notifizierte Stellen und Marktüberwachungsbehörden.
Biostimulants des végétaux - Allégations - Partie 3 : Tolérance au stress abiotique résultant de l’utilisation d’un biostimulant des végétaux
Le présent document fournit des recommandations pour justifier l’allégation de tolérance au stress abiotique des biostimulants des végétaux utilisés en agriculture.
Le présent document est principalement destiné aux fabricants, aux laboratoires, aux sociétés qui vont mettre les produits sur le marché, aux autorités notifiantes, aux organismes notifiés et aux autorités de surveillance du marché.
Rastlinski biostimulanti - Navedbe - 3. del: Toleranca na abiotični stres pri rastlinah zaradi uporabe biostimulanta
Ta dokument podaja smernice za utemeljitev navedb o toleranci na abiotični stres zaradi uporabe rastlinskih biostimulantov v kmetijstvu.
Ta dokument je namenjen zlasti proizvajalcem, laboratorijem, podjetjem, ki bodo dala izdelke na trg, priglasitvenim organom, priglašenim organom in organom za nadzor trga.
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
01-januar-2023
Rastlinski biostimulanti - Navedbe - 3. del: Toleranca na abiotični stres pri
rastlinah zaradi uporabe biostimulanta
Plant biostimulants - Claims - Part 3: Tolerance to abiotic stress resulting from the use of
a plant biostimulant
Pflanzen-Biostimulanzien - Auslobungen - Teil 3: Toleranz gegenüber abiotischem Stress
infolge der Verwendung eines Pflanzen-Biostimulans
Biostimulants des végétaux - Allégations - Partie 3 : Tolérance au stress abiotique
résultant de l’utilisation d’un biostimulant des végétaux
Ta slovenski standard je istoveten z: CEN/TS 17700-3:2022
ICS:
65.080 Gnojila Fertilizers
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
CEN/TS 17700-3
TECHNICAL SPECIFICATION
SPÉCIFICATION TECHNIQUE
March 2022
TECHNISCHE SPEZIFIKATION
ICS 65.080
English Version
Plant biostimulants - Claims - Part 3: Tolerance to abiotic
stress resulting from the use of a plant biostimulant
Biostimulants des végétaux - Allégations - Partie 3 : Biostimulanzien für die pflanzliche Anwendung -
Tolérance au stress abiotique résultant de l'utilisation Angaben - Toleranz gegenüber abiotischem Stress
d'un biostimulant des végétaux infolge der Verwendung eines Biostimulans für die
pflanzliche Anwendung
This Technical Specification (CEN/TS) was approved by CEN on 3 January 2022 for provisional application.
The period of validity of this CEN/TS is limited initially to three years. After two years the members of CEN will be requested to
submit their comments, particularly on the question whether the CEN/TS can be converted into a European Standard.
CEN members are required to announce the existence of this CEN/TS in the same way as for an EN and to make the CEN/TS
available promptly at national level in an appropriate form. It is permissible to keep conflicting national standards in force (in
parallel to the CEN/TS) until the final decision about the possible conversion of the CEN/TS into an EN is reached.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2022 CEN All rights of exploitation in any form and by any means reserved Ref. No. CEN/TS 17700-3:2022 E
worldwide for CEN national Members.
Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative references . 5
3 Terms and definitions . 5
4 Terminology of the claim . 6
5 Justification of plant biostimulant claims regarding abiotic stress tolerance . 7
5.1 Introduction . 7
5.2 Trial design with negative control . 7
5.3 Trial design without negative control . 8
6 Agronomic markers to validate the claim . 9
7 Specifications for the performance of the trials .11
European foreword
This document (CEN/TS 17700-3:2022) has been prepared by Technical Committee CEN/TC 455 “Plant
Biostimulants”, the secretariat of which is held by AFNOR.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document has been prepared under a Standardization Request given to CEN by the European
Commission and the European Free Trade Association.
The CEN/TS 17700 series, Plant biostimulants - Claims, consists of the following parts:
— Part 1: General principles;
— Part 2: Nutrient use efficiency resulting from the use of a plant biostimulant;
— Part 3: Tolerance to abiotic stress resulting from the use of a plant biostimulant;
— Part 4: Determination of quality traits resulting from the use of a plant biostimulant;
— Part 5: Determination of availability of confined nutrient in the soil or rhizosphere.
Any feedback and questions on this document should be directed to the users’ national standards body.
A complete listing of these bodies can be found on the CEN website.
According to the CEN/CENELEC Internal Regulations, the national standards organisations of the
following countries are bound to announce this Technical Specification: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United
Kingdom.
Introduction
This document has been developed to provide guidance for a consistent approach to justify the claims
associated with the use of plant biostimulants in agriculture.
The definition of plant biostimulants to be used in the regulation on fertilizing materials is
claims-based. For this reason, demonstrating that a product is indeed a bona fide plant biostimulant
depends on a demonstration of its effect.
The placing of a plant biostimulant on the market should not be considered to guarantee effectiveness
under all conditions, as many factors may influence the performance of a plant biostimulant in the field.
Plant biostimulants used in agriculture can be applied in multiple ways: on soil, on plant, as seed
treatment, etc. This document is applicable to all application types of plant biostimulants in agriculture.
1 Scope
This document provides guidance for justifying abiotic stress tolerance claim of plant biostimulants used
in agriculture.
This document is aimed primarily at manufacturers, laboratories, companies which will put the products
on the market, notifying authorities, notified bodies, and market surveillance authorities.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
CEN/TS 17700-1:2022, Plant biostimulants - Claims - Part 1: General principles
CEN/TS 17724, Plant biostimulants - Terminology
3 Terms and definitions
For the purposes of this document, the terms and definitions given in CEN/TS 17700-1, CEN/TS 17724
and the following apply.
3.1
tolerance to abiotic stress
ability to endure abiotic stress
3.2
abiotic stress
negative impact of non-living factors on the plant in a specific crop environment
Note 1 to entry: Crop tolerance to abiotic stress is addressed to one or more (multiple or combined) of the following
stress categories:
1) thermal stress,
2) light stress,
3) mechanical stress,
4) water stress,
5) chemical stress.
3.3
thermal stress
negative impact of temperature (supra-optimal and sub-optimal temperature) on the plant in a specific
crop environment
EXAMPLE heat stress or cold stress such as chilling and freezing stress
3.4
light stress
negative impact of light intensity and/or spectrum on the plant in a specific crop environment
EXAMPLE high irradiance or low irradiance, UV radiation
3.5
mechanical stress
negative impact of a mechanical force on the plant or the root zone in a specific crop environment
EXAMPLE wind, hail, agricultural operations
3.6
water stress
negative impact of water or high solutes concentrations (supra-optimal and sub-optimal water level) or
excessive transpiration on the plant in a specific crop environment
EXAMPLE drought, high air vapour pressure deficit, flooding
3.7
chemical stress
negative impact of chemicals (supra-optimal or sub-optimal chemical compounds or presence) on the
plant in a specific crop environment
EXAMPLE salt stress, mineral toxicity induced by heavy metals or excessive application of mineral nutrients,
adverse pH conditions, ozone stress, phytotoxic effects of xenobiotics
3.8
xenobiotic
chemical substance found within an organism that is not naturally produced or expected to be present
within the organism
EXAMPLE heavy metals, pesticides, ozone
4 Terminology of the claim
The type of claim(s) included in the Technical Specification can be addressed to one or more of the above
defined abiotic stress(es) – see 3.3 to 3.7.
For instance, a plant biostimulant can improve plant tolerance to a single abiotic stress factor such as
heat stress or to multiple abiotic stress factors such as heat stress and salt stress in one or more target
crop(s).
Label shall clearly indicate that the plant biostimulant is addressed to improve tolerance to abiotic stress
with a clear indication of the type of abiotic stress(es) and the crop(s) on which the effect is demonstrated
according to CEN/TS 17700-1.
EXAMPLE “improves tolerance to drought stress on woody perennials”, “improves tolerance to cold stress on
tomato”
5 Justification of plant biostimulant claims regarding abiotic stress tolerance
5.1 Introduction
In open field conditions it can be difficult to fully control specific abiotic stress factors due to their
inherent variability and due to the fact, that, often several abiotic stresses may be present at the same
time (combinational stresses). Therefore, to fully validate an abiotic stress tolerance claim with both a
negative (untreated and unstressed) control and a positive (untreated and stressed) control it should
take place under controlled conditions (e.g. growth chamber, growth room, greenhouse).
In controlled conditions or where a negative and positive control can be easily implemented, contrasting,
and comparing both these groups, allows a researcher to assess the impact and validity of the stress
model. Or in other words, how much damage/growth depression was caused by the stress prior to any
intervention (biostimulant application).
Depending on how tangible (easy to control or easy to isolate) the abiotic stress being examined is,
sometimes a negative control and a positive control may be implemented in open field conditions.
However, as stated previously, in some situations a negative control might not be easily implemented. In
this case, the emphasis is placed more on detecting the specific stress by assessing stress specific markers
within the plant response to both the environment (growing conditions) and the plant biostimulant
application.
In order to justify the claim, these stress markers shall be of agronomic significance (specific to the stress
being examined e.g. heat shock proteins created by heat stress). Their relevance may be proved through
referencing previous peer reviewed literature that correlated these markers to the stress being
examined. Often this stress marker (be it physiological, biochemical or genetic) will cause a plant
response (change in phenotype) which will either be promoted or reduced through the use of
biostimulants.
Therefore, assessing the stress marker in both the positive control (untreated and stressed) versus the
treated (product to be tested) group, will allow a researcher to assess the presence of the stress and how
much of a change was caused in the plant response by applying the product, allowing trials without a
negative control to validate abiotic stress tolerance.
5.2 Trial design with negative control
For the above reasons, the trial should include at a minimum the following three treatments:
Treatment Stress conditions Non-stress conditions
Positive Control, untreated plant (X) ●
Tested Product, treated plant (Y) ●
Negative Control, untreated plant (Z) ●
In context with the above table, comparing the positive and negative controls (X vs Z) provided all other
variables are held equal will demonstrate that the “abiotic stress” in question is real.
Positive Control (X) and Tested Product (Y) treatments can be compared only if the duration and intensity
of abiotic stress is the same. All other factors such as genotype, cultural practices and environmental
conditions beyond the selected stress factor shall be the same for all treatments.
In the case of a plant trait (i.e. plant biomass - A) that is reduced with the increase of abiotic stress level,
the claim is validated for the tested product when Y and Z are significantly higher (refer to
A A
CEN/TS 17700-1:2022, Clause 5) than X .
A
...
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