Wood preservatives - Determination of the protective effectiveness against Anobium punctatum (De Geer) by egg-laying and larval survival - Part 2: Application by impregnation (Laboratory method)

This European Standard specifies a method for the determination of the protective effectiveness or the toxic values of a wood preservative against Anobium punctatum (De Geer) by egg-laying and larval survival in wood which has been treated previously by full impregnation. This method is applicable to:
-   water-insoluble chemicals which are being studied as active insecticides;
-   organic formulations, as supplied or as prepared in the laboratory by dilution of concentrates;
-   organic water-dispersible formulations as supplied or as prepared in the laboratory by dilution of concentrates, and
-   water-soluble materials, for example salts.
NOTE   This method can be used in conjunction with an ageing procedure, for example EN 73.

Holzschutzmittel - Bestimmung der vorbeugenden Wirkung gegenüber Anobium punctatum (De Geer) durch Beobachten der Eiablage und des Überlebens von Larven - Teil 2: Anwendung durch Volltränkung (Laboratoriumsverfahren)

Diese Europäische Norm legt ein Verfahren zur Bestimmung der vorbeugenden Wirkung oder der Grenze der Wirksamkeit eines Holzschutzmittels gegenüber Anobium punctatum (De Geer) durch Beobachten der Eiablage und des Überlebens der Larven in einem zuvor durch Volltränkung behandelten Holz fest. Das Verfahren gilt für:
-   wasserunlösliche Chemikalien, die als wirksame Insektizide untersucht werden;
-   organische Formulierungen in deren Anlieferungszustand oder in Form von im Laboratorium hergestellten Verdünnungen von Konzentraten;
-   organische wasserdispergierbare Formulierungen in deren Anlieferungszustand oder in Form von im Laboratorium hergestellten Verdünnungen von Konzentraten; und
-   wasserlösliche Stoffe, zum Beispiel Salze.
ANMERKUNG   Das Verfahren kann in Verbindung mit einer geeigneten Alterungsbeanspruchung, zum Beispiel nach EN 73, angewendet werden.

Produits de préservation du bois - Détermination de l'efficacité protectrice vis à vis de Anobium punctatum (De Geer) par l'observation de la ponte et de la survie des larves - Partie 2 : Application par imprégnation (Méthode de laboratoire)

La présente Norme européenne prescrit une méthode de détermination de l’efficacité protectrice ou du seuil d’efficacité d’un produit de préservation du bois vis-à-vis d’Anobiurn punctatum (De Geer) par observation de la ponte et de la survie des larves dans du bois préalablement traité par imprégnation totale. La présente méthode est applicable :
-   aux produits chimiques non hydrosolubles étudiés en tant que matières actives insecticides ;
-   aux formulations organiques telles qu’elles sont fournies ou préparées au laboratoire à partir de concentrés ;
-   aux formulations organiques hydrodispersables telles qu’elles sont fournies ou préparées au laboratoire à partir de concentrés ; et
-   aux produits hydrosolubles, par exemple aux sels.
NOTE   Cette méthode peut être utilisée conjointement avec une épreuve de vieillissement, par exemple l’EN 73.

Biocidni proizvodi za zaščito lesa - Ugotavljanje učinkovitosti preventivne zaščite proti navadnemu trdoglavcu Anobium punctatum (De Geer) glede na število položenih jajčec in preživelih ličink - 2. del: Postopek z globinsko impregnacijo lesa (laboratorijska metoda)

Ta dokument določa metodo za ugotavljanje učinkovitosti preventivne zaščite ali toksičnih vrednosti zaščitnega sredstva za les proti navadnemu trdoglavcu Anobium punctatum (De Geer) glede na število položenih jajčec in preživelih ličink v lesu, ki je bil obdelan s popolno impregnacijo. Ta metoda se uporablja za: ¾ v vodi netopne kemikalije, ki se preučujejo kot aktivni insekticidi, ¾ organske formulacije, ki so dobavljene ali pripravljene v laboratoriju z redčenjem koncentratov, ¾ organske formulacije, ki dispergirajo v vodi in so dobavljene ali pripravljene v laboratoriju z redčenjem koncentratov ter ¾ v vodi topne materiale, na primer sol. OPOMBA: Ta metoda se lahko uporablja v povezavi s postopkom staranja, na primer EN 73.

General Information

Status
Published
Publication Date
18-Aug-2015
Withdrawal Date
28-Feb-2016
Current Stage
9092 - Decision on results of review/2YR ENQ - revise - Review Enquiry
Completion Date
15-Dec-2021

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SLOVENSKI STANDARD
01-oktober-2015
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SIST EN 49-2:2005
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Wood preservatives - Determination of the protective effectiveness against Anobium
punctatum (De Geer) by egg-laying and larval survival - Part 2: Application by
impregnation (Laboratory method)
Holzschutzmittel - Bestimmung der vorbeugenden Wirkung gegenüber Anobium
punctatum (De Geer) durch Beobachten der Eiablage und des Überlebens von Larven -
Teil 2: Anwendung durch Volltränkung (Laboratoriumsverfahren)
Produits de préservation du bois - Détermination de l'efficacité protectrice vis à vis de
Anobium punctatum (De Geer) par l'observation de la ponte et de la survie des larves -
Partie 2 : Application par imprégnation (Méthode de laboratoire)
Ta slovenski standard je istoveten z: EN 49-2:2015
ICS:
71.100.50 .HPLNDOLMH]D]DãþLWROHVD Wood-protecting chemicals
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EUROPEAN STANDARD
EN 49-2
NORME EUROPÉENNE
EUROPÄISCHE NORM
August 2015
ICS 71.100.50 Supersedes EN 49-2:2005
English Version
Wood preservatives - Determination of the protective
effectiveness against Anobium punctatum (De Geer) by egg-
laying and larval survival - Part 2: Application by impregnation
(Laboratory method)
Produits de préservation du bois - Détermination de Holzschutzmittel - Bestimmung der vorbeugenden Wirkung
l'efficacité protectrice vis à vis de Anobium punctatum (De gegenüber Anobium punctatum (De Geer) durch
Geer) par l'observation de la ponte et de la survie des Beobachten der Eiablage und des Überlebens von Larven -
larves - Partie 2 : Application par imprégnation (Méthode de Teil 2: Anwendung durch Volltränkung
laboratoire) (Laboratoriumsverfahren)
This European Standard was approved by CEN on 16 July 2015.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same
status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United
Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2015 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 49-2:2015 E
worldwide for CEN national Members.

Contents Page
European foreword .3
Introduction .4
1 Scope .5
2 Normative reference .5
3 Terms and definitions .5
4 Principle .5
5 Test materials .6
5.1 Biological material .6
5.2 Products and reagents .6
5.3 Apparatus .6
6 Sampling .7
7 Test specimens .8
7.1 Species of wood.8
7.2 Wood quality .8
7.3 Provision of test specimens .8
7.4 Dimensions of test specimens .8
7.5 Number of test specimens .8
8 Procedure .9
8.1 Preparation of the test specimens .9
8.1.1 Conditioning of test specimens before treatment .9
8.1.2 Treatment of the test specimens .9
8.1.3 Drying and conditioning of the test specimens after treatment . 10
8.2 Exposure of the test specimens to the insects . 11
8.3 Conditions and duration of the test . 11
8.4 Examination of the test specimens . 11
9 Validity of test . 12
10 Expression of results . 12
10.1 Assessment of the protective effectiveness . 12
10.2 Toxic values . 12
11 Test report . 12
Annex A (informative) Example of a test report . 14
Annex B (informative) Identification of sex of test insects (Anobium punctatum) . 16
Annex C (informative) Culturing technique for Anobium punctatum . 17
Annex D (informative) Environmental, health and safety precautions within chemical/biological
laboratory . 20
Bibliography . 21

European foreword
This document (EN 49-2:2015) has been prepared by Technical Committee CEN/TC 38 “Durability of wood
and wood-based products”, the secretariat of which is held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by February 2016 and conflicting national standards shall be withdrawn
at the latest by February 2016.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN 49-2:2005.
Significant technical differences between this document and EN 49-2:2005 are as follows:
a) generalization of material for preparing the egg-laying zones;
b) introduction of new harmonized specifications for wood quality.
EN 49, Wood preservatives ― Determination of the protective effectiveness against Anobium punctatum (De
Geer) by egg-laying and larval survival, consists of two parts:
— Part 1: Application by surface treatment (Laboratory method);
— Part 2: Application by impregnation (Laboratory method).
EN 49-1 is required to enable effectiveness assessments of wood preservatives which are intended to be
applied by surface treatment and EN 49-2 those which are intended to be applied by impregnation.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech
Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece,
Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom.
Introduction
This document describes a laboratory method of testing which gives a basis for assessment of the
effectiveness of a wood preservative, against Anobium punctatum. It allows the determination of the
concentration at which the product prevents the development of infestation from egg laying.
The method simulates conditions which can occur in practice on timber which has been treated some time
previously with a deeply penetrating wood preservative and on which eggs of Anobium punctatum are laid.
This laboratory method provides one criterion by which the value of a product can be assessed. In making this
assessment the methods by which the preservative may be applied should be taken into account. It is further
recommended that results from this test should be supplemented by those from other appropriate tests, and
above all by comparison with practical experience.
When products which are very active at low concentrations are used it is very important to take suitable
precautions to isolate and separate, as far as possible, operations involving chemical products, other
products, treated wood, laboratory apparatus and clothing. Suitable precautions should include the use of
separate rooms, areas within rooms, extraction facilities, conditioning chambers and special training for
personnel (see also Annex D for environmental, health and safety precautions).
1 Scope
This European Standard specifies a method for the determination of the protective effectiveness or the toxic
values of a wood preservative against Anobium punctatum (De Geer) by egg-laying and larval survival in
wood which has been treated previously by full impregnation. This method is applicable to:
— water-insoluble chemicals which are being studied as active insecticides;
— organic formulations, as supplied or as prepared in the laboratory by dilution of concentrates;
— organic water-dispersible formulations as supplied or as prepared in the laboratory by dilution of
concentrates, and
— water-soluble materials, for example salts.
NOTE This method can be used in conjunction with an ageing procedure, for example EN 73.
2 Normative reference
The following documents, in whole or in part, are normatively referenced in this document and are
indispensable for its application. For dated references, only the edition cited applies. For undated references,
the latest edition of the referenced document (including any amendments) applies.
EN ISO 3696, Water for analytical laboratory use - Specification and test methods (ISO 3696)
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1
representative sample
sample having its physical or chemical characteristics identical to the volumetric average characteristics of the
total volume being sampled
3.2
supplier
sponsor of the test (person or company providing the sample of wood preservative to be tested)
4 Principle
The treated test specimens are exposed to gravid females of Anobium punctatum. The numbers of eggs laid,
the numbers of eggs hatched, and the numbers of the surviving larvae are compared with those in untreated
control test specimens. If the preservative has been prepared in the laboratory by dilution of a concentrate or
by dissolution of a solid, the resulting attack is also compared to that in solvent or diluent treated control test
specimens.
Depending on the test being carried out either
— on a set of test specimens of a susceptible wood species that is impregnated with a solution of the
preservative, or
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