Wood preservatives - Determination of the protective effectiveness against Anobium punctatum (De Geer) by egg-laying and larval survival - Part 2: Application by impregnation (Laboratory method)

This European Standard specifies a method for the determination of the protective effectiveness or the toxic values of a wood preservative against Anobium punctatum (De Geer) by egg-laying and larval survival in wood which has been treated previously by full impregnation. This method is applicable to:
-   water-insoluble chemicals which are being studied as active insecticides;
-   organic formulations, as supplied or as prepared in the laboratory by dilution of concentrates;
-   organic water-dispersible formulations as supplied or as prepared in the laboratory by dilution of concentrates, and
-   water-soluble materials, for example salts.
NOTE   This method can be used in conjunction with an ageing procedure, for example EN 73.

Holzschutzmittel - Bestimmung der vorbeugenden Wirkung gegenüber Anobium punctatum (De Geer) durch Beobachten der Eiablage und des Überlebens von Larven - Teil 2: Anwendung durch Volltränkung (Laboratoriumsverfahren)

Diese Europäische Norm legt ein Verfahren zur Bestimmung der vorbeugenden Wirkung oder der Grenze der Wirksamkeit eines Holzschutzmittels gegenüber Anobium punctatum (De Geer) durch Beobachten der Eiablage und des Überlebens der Larven in einem zuvor durch Volltränkung behandelten Holz fest. Das Verfahren gilt für:
-   wasserunlösliche Chemikalien, die als wirksame Insektizide untersucht werden;
-   organische Formulierungen in deren Anlieferungszustand oder in Form von im Laboratorium hergestellten Verdünnungen von Konzentraten;
-   organische wasserdispergierbare Formulierungen in deren Anlieferungszustand oder in Form von im Laboratorium hergestellten Verdünnungen von Konzentraten; und
-   wasserlösliche Stoffe, zum Beispiel Salze.
ANMERKUNG   Das Verfahren kann in Verbindung mit einer geeigneten Alterungsbeanspruchung, zum Beispiel nach EN 73, angewendet werden.

Produits de préservation du bois - Détermination de l'efficacité protectrice vis à vis de Anobium punctatum (De Geer) par l'observation de la ponte et de la survie des larves - Partie 2 : Application par imprégnation (Méthode de laboratoire)

La présente Norme européenne prescrit une méthode de détermination de l’efficacité protectrice ou du seuil d’efficacité d’un produit de préservation du bois vis-à-vis d’Anobiurn punctatum (De Geer) par observation de la ponte et de la survie des larves dans du bois préalablement traité par imprégnation totale. La présente méthode est applicable :
-   aux produits chimiques non hydrosolubles étudiés en tant que matières actives insecticides ;
-   aux formulations organiques telles qu’elles sont fournies ou préparées au laboratoire à partir de concentrés ;
-   aux formulations organiques hydrodispersables telles qu’elles sont fournies ou préparées au laboratoire à partir de concentrés ; et
-   aux produits hydrosolubles, par exemple aux sels.
NOTE   Cette méthode peut être utilisée conjointement avec une épreuve de vieillissement, par exemple l’EN 73.

Biocidni proizvodi za zaščito lesa - Ugotavljanje učinkovitosti preventivne zaščite proti navadnemu trdoglavcu Anobium punctatum (De Geer) glede na število položenih jajčec in preživelih ličink - 2. del: Postopek z globinsko impregnacijo lesa (laboratorijska metoda)

Ta dokument določa metodo za ugotavljanje učinkovitosti preventivne zaščite ali toksičnih vrednosti zaščitnega sredstva za les proti navadnemu trdoglavcu Anobium punctatum (De Geer) glede na število položenih jajčec in preživelih ličink v lesu, ki je bil obdelan s popolno impregnacijo. Ta metoda se uporablja za: ¾ v vodi netopne kemikalije, ki se preučujejo kot aktivni insekticidi, ¾ organske formulacije, ki so dobavljene ali pripravljene v laboratoriju z redčenjem koncentratov, ¾ organske formulacije, ki dispergirajo v vodi in so dobavljene ali pripravljene v laboratoriju z redčenjem koncentratov ter ¾ v vodi topne materiale, na primer sol. OPOMBA: Ta metoda se lahko uporablja v povezavi s postopkom staranja, na primer EN 73.

General Information

Status
Published
Publication Date
18-Aug-2015
Current Stage
9060 - Closure of 2 Year Review Enquiry - Review Enquiry
Due Date
04-Mar-2021
Completion Date
04-Mar-2021

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SLOVENSKI STANDARD
SIST EN 49-2:2015
01-oktober-2015
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SIST EN 49-2:2005

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SURWLQDYDGQHPXWUGRJODYFX$QRELXPSXQFWDWXP 'H*HHU JOHGHQDãWHYLOR

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Wood preservatives - Determination of the protective effectiveness against Anobium

punctatum (De Geer) by egg-laying and larval survival - Part 2: Application by
impregnation (Laboratory method)
Holzschutzmittel - Bestimmung der vorbeugenden Wirkung gegenüber Anobium

punctatum (De Geer) durch Beobachten der Eiablage und des Überlebens von Larven -

Teil 2: Anwendung durch Volltränkung (Laboratoriumsverfahren)

Produits de préservation du bois - Détermination de l'efficacité protectrice vis à vis de

Anobium punctatum (De Geer) par l'observation de la ponte et de la survie des larves -

Partie 2 : Application par imprégnation (Méthode de laboratoire)
Ta slovenski standard je istoveten z: EN 49-2:2015
ICS:
71.100.50 .HPLNDOLMH]D]DãþLWROHVD Wood-protecting chemicals
SIST EN 49-2:2015 en,fr,de

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN 49-2:2015
---------------------- Page: 2 ----------------------
SIST EN 49-2:2015
EUROPEAN STANDARD
EN 49-2
NORME EUROPÉENNE
EUROPÄISCHE NORM
August 2015
ICS 71.100.50 Supersedes EN 49-2:2005
English Version
Wood preservatives - Determination of the protective
effectiveness against Anobium punctatum (De Geer) by egg-
laying and larval survival - Part 2: Application by impregnation
(Laboratory method)

Produits de préservation du bois - Détermination de Holzschutzmittel - Bestimmung der vorbeugenden Wirkung

l'efficacité protectrice vis à vis de Anobium punctatum (De gegenüber Anobium punctatum (De Geer) durch

Geer) par l'observation de la ponte et de la survie des Beobachten der Eiablage und des Überlebens von Larven -

larves - Partie 2 : Application par imprégnation (Méthode de Teil 2: Anwendung durch Volltränkung

laboratoire) (Laboratoriumsverfahren)
This European Standard was approved by CEN on 16 July 2015.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European

Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national

standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by translation

under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same

status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,

Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,

Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United

Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels

© 2015 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 49-2:2015 E

worldwide for CEN national Members.
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SIST EN 49-2:2015
EN 49-2:2015 (E)
Contents Page

European foreword .............................................................................................................................................3

Introduction .........................................................................................................................................................4

1 Scope ......................................................................................................................................................5

2 Normative reference ..............................................................................................................................5

3 Terms and definitions ...........................................................................................................................5

4 Principle ..................................................................................................................................................5

5 Test materials .........................................................................................................................................6

5.1 Biological material .................................................................................................................................6

5.2 Products and reagents ..........................................................................................................................6

5.3 Apparatus ...............................................................................................................................................6

6 Sampling .................................................................................................................................................7

7 Test specimens ......................................................................................................................................8

7.1 Species of wood.....................................................................................................................................8

7.2 Wood quality ..........................................................................................................................................8

7.3 Provision of test specimens .................................................................................................................8

7.4 Dimensions of test specimens .............................................................................................................8

7.5 Number of test specimens ....................................................................................................................8

8 Procedure ...............................................................................................................................................9

8.1 Preparation of the test specimens .......................................................................................................9

8.1.1 Conditioning of test specimens before treatment ..............................................................................9

8.1.2 Treatment of the test specimens ..........................................................................................................9

8.1.3 Drying and conditioning of the test specimens after treatment .................................................... 10

8.2 Exposure of the test specimens to the insects ............................................................................... 11

8.3 Conditions and duration of the test .................................................................................................. 11

8.4 Examination of the test specimens ................................................................................................... 11

9 Validity of test ..................................................................................................................................... 12

10 Expression of results ......................................................................................................................... 12

10.1 Assessment of the protective effectiveness .................................................................................... 12

10.2 Toxic values ........................................................................................................................................ 12

11 Test report ........................................................................................................................................... 12

Annex A (informative) Example of a test report ............................................................................................ 14

Annex B (informative) Identification of sex of test insects (Anobium punctatum) ................................... 16

Annex C (informative) Culturing technique for Anobium punctatum ......................................................... 17

Annex D (informative) Environmental, health and safety precautions within chemical/biological

laboratory ............................................................................................................................................ 20

Bibliography ..................................................................................................................................................... 21

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SIST EN 49-2:2015
EN 49-2:2015 (E)
European foreword

This document (EN 49-2:2015) has been prepared by Technical Committee CEN/TC 38 “Durability of wood

and wood-based products”, the secretariat of which is held by AFNOR.

This European Standard shall be given the status of a national standard, either by publication of an identical

text or by endorsement, at the latest by February 2016 and conflicting national standards shall be withdrawn

at the latest by February 2016.

Attention is drawn to the possibility that some of the elements of this document may be the subject of patent

rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.

This document supersedes EN 49-2:2005.

Significant technical differences between this document and EN 49-2:2005 are as follows:

a) generalization of material for preparing the egg-laying zones;
b) introduction of new harmonized specifications for wood quality.

EN 49, Wood preservatives ― Determination of the protective effectiveness against Anobium punctatum (De

Geer) by egg-laying and larval survival, consists of two parts:
— Part 1: Application by surface treatment (Laboratory method);
— Part 2: Application by impregnation (Laboratory method).

EN 49-1 is required to enable effectiveness assessments of wood preservatives which are intended to be

applied by surface treatment and EN 49-2 those which are intended to be applied by impregnation.

According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following

countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech

Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece,

Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,

Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom.

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SIST EN 49-2:2015
EN 49-2:2015 (E)
Introduction

This document describes a laboratory method of testing which gives a basis for assessment of the

effectiveness of a wood preservative, against Anobium punctatum. It allows the determination of the

concentration at which the product prevents the development of infestation from egg laying.

The method simulates conditions which can occur in practice on timber which has been treated some time

previously with a deeply penetrating wood preservative and on which eggs of Anobium punctatum are laid.

This laboratory method provides one criterion by which the value of a product can be assessed. In making this

assessment the methods by which the preservative may be applied should be taken into account. It is further

recommended that results from this test should be supplemented by those from other appropriate tests, and

above all by comparison with practical experience.

When products which are very active at low concentrations are used it is very important to take suitable

precautions to isolate and separate, as far as possible, operations involving chemical products, other

products, treated wood, laboratory apparatus and clothing. Suitable precautions should include the use of

separate rooms, areas within rooms, extraction facilities, conditioning chambers and special training for

personnel (see also Annex D for environmental, health and safety precautions).
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SIST EN 49-2:2015
EN 49-2:2015 (E)
1 Scope

This European Standard specifies a method for the determination of the protective effectiveness or the toxic

values of a wood preservative against Anobium punctatum (De Geer) by egg-laying and larval survival in

wood which has been treated previously by full impregnation. This method is applicable to:

— water-insoluble chemicals which are being studied as active insecticides;

— organic formulations, as supplied or as prepared in the laboratory by dilution of concentrates;

— organic water-dispersible formulations as supplied or as prepared in the laboratory by dilution of

concentrates, and
— water-soluble materials, for example salts.

NOTE This method can be used in conjunction with an ageing procedure, for example EN 73.

2 Normative reference

The following documents, in whole or in part, are normatively referenced in this document and are

indispensable for its application. For dated references, only the edition cited applies. For undated references,

the latest edition of the referenced document (including any amendments) applies.

EN ISO 3696, Water for analytical laboratory use - Specification and test methods (ISO 3696)

3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1
representative sample

sample having its physical or chemical characteristics identical to the volumetric average characteristics of the

total volume being sampled
3.2
supplier

sponsor of the test (person or company providing the sample of wood preservative to be tested)

4 Principle

The treated test specimens are exposed to gravid females of Anobium punctatum. The numbers of eggs laid,

the numbers of eggs hatched, and the numbers of the surviving larvae are compared with those in untreated

control test specimens. If the preservative has been prepared in the laboratory by dilution of a concentrate or

by dissolution of a solid, the resulting attack is also compared to that in solvent or diluent treated control test

specimens.
Depending on the test being carried out either

— on a set of test specimens of a susceptible wood species that is impregnated with a solution of the

preservative, or
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SIST EN 49-2:2015
EN 49-2:2015 (E)

— if toxic values are to be determined, on several sets of test specimens of a susceptible wood species that

are impregnated with a series of solutions in which the concentration of preservative is ranged in a given

progression.
5 Test materials
5.1 Biological material
Anobium punctatum (De Geer)
Adult males and females in good condition.

Adults to be used in the test shall be collected daily from naturally infested wood or laboratory culture (see

Annex C).

Use recently emerged adults which have been recently collected; kept overnight in quarantine (see C.2.2 and

C.6) and then checked to ensure that they are undamaged, active, and free from any infestation by mites.

Determine the sex (see Annex B) of the collected and checked adults and place the males and females in

separate containers.
NOTE The proportion of males and females varies during the emergence period.
5.2 Products and reagents
5.2.1 Paraffin wax, for sealing the end sections of test specimens.

NOTE Paraffin wax with a setting point of 52 °C to 54 °C has been found to be suitable.

5.2.2 Paste, for securing filter paper. The paste shall be starch-free, non-toxic to Anobium punctatum and

insoluble in the product under test.
NOTE Sodium carboxymethyl cellulose, food grade, has been found to be suitable.
5.2.3 Xylene, technical grade, mixed isomers.
5.2.4 Water, complying with grade 3 of EN ISO 3696.

5.2.5 Solvent or diluent, a volatile liquid that will dissolve or dilute the preservative but does not leave a

residue in the wood at the end of the post-treatment conditioning period that has a toxic effect on the insects.

CAUTION — Do not use benzene or other solvents which pose a health risk.
5.2.6 Filter paper, ordinary quality, medium-fast grade.

5.2.7 Fine cloth of a suitable material with a mesh aperture of 0,3 mm to 0,6 mm for the preparation of the

egg-laying zones.
NOTE Cotton, linen and polyamide-gauze have been proven suitable.
5.3 Apparatus

5.3.1 Culturing chamber, with air circulation, controlled at (21 ± 2) °C, and at relative humidity (80 ± 5) %.

5.3.2 Conditioning chamber, well ventilated, controlled at (20 ± 2) °C and at relative humidity (65 ± 5) %.

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SIST EN 49-2:2015
EN 49-2:2015 (E)

The conditioning of test specimens may be carried out in the laboratory work area (see 5.3.4) provided that

this has the conditions specified for the conditioning chamber (see 5.3.2).

5.3.3 Treatment vessel(s), of a material that does not react with the preservative under test, for example of

glass for organic products and of polyethylene for salts containing fluorine.

5.3.4 Laboratory work area, well ventilated, where treatment of the test specimens is carried out.

CAUTION — It is essential to follow safety procedures for handling flammable and toxic materials.

Avoid excessive exposure of operators to solvents or their vapours.

5.3.5 Testing chamber, with conditions identical to those of the culturing chamber (see 5.3.1).

5.3.6 Drying vessel(s), capable of holding sets of five test specimens (7.5), provided with a close-fitting

cover and containing supports that will give minimum contact with treated test specimens to be placed on

them. The vessels and supports shall be of a material that does not react with the preservative under test, for

example glass for organic compounds and polyethylene for products containing fluorine.

5.3.7 Vacuum vessel(s), fitted with stopcocks, capable of receiving the treatment vessels (5.3.3).

5.3.8 Vacuum pump, fitted with a pressure gauge and capable of maintaining a pressure of 700 Pa

5.3.9 Weights, to provide ballast for the test specimens. The weights shall not react with any materials with

which they come into contact during the test.

5.3.10 Safety equipment and protective clothing and gloves, appropriate for the test product and the test

solvent, to ensure the safety of the operator.

5.3.11 Test containers, suitable for holding the test specimens and of material resistant to the solvents

used, and fitted with perforated covers to provide a good exchange of air.

NOTE Jars of approximately 60 mm diameter and 100 mm height have been found to be suitable.

5.3.12 Ordinary laboratory equipment, including a balance capable of weighing to an accuracy of 0,01 g.

5.3.13 X-ray apparatus, (optional) with tungsten-target and beryllium window, with voltage and current

continuously variable in the ranges:
— voltage: 10 kV to 50 kV;
— current: 0 mA to 15 mA.
6 Sampling

The sample of preservative shall be representative of the product to be tested. Samples shall be stored and

handled in accordance with any written recommendations from the supplier.

For the sampling of preservatives from bulk supplies, the procedure given in EN 212 should be used.

1) 100 Pa = 1 mbar.
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SIST EN 49-2:2015
EN 49-2:2015 (E)
7 Test specimens
7.1 Species of wood

The reference species is European oak. This shall be either sessile oak (Quercus petraea (Mattuschka)

Lieblin) or pedunculate oak (Quercus robur Linnaeus).

Additional tests may be carried out using other timber species but, if so, this should be stated in the test

report.
7.2 Wood quality

The wood shall be free from visible cracks, stain, decay, insect damage and other defects. The wood shall not

have been water-stored, floated, chemically treated or steamed. The wood shall originate from trees

preferably felled in winter. The trees shall be cut immediately after felling and the timber rapidly air-dried or

kiln dried at temperatures below 60 °C. The wood shall not be stored for more than five years.

The wood shall be exclusively sapwood and having between two annual rings per 10 mm and 10 annual

rings per 10 mm.

It is recommended to use test specimens of similar growth rate within a single test.

7.3 Provision of test specimens

Prepare planed strips having a cross-section of (25 ± 0,5) mm × (15 ± 0,5) mm removing a minimum of

2 mm from any surfaces exposed during drying. The longitudinal faces shall be parallel to the direction of the

grain. The annual rings shall be parallel to the broad faces (contact angle of less than 5°). Make transverse

cuts, neatly to give sharp edges and a fine-sawn finish to the end-grain surfaces, to give test specimens

(50 ± 0,5) mm long.

The test specimens shall originate from a minimum of three trees or shall be taken at random from a stock

originally of more than 500 test specimens.
7.4 Dimensions of test specimens

The dimensions of each test specimen after reaching equilibrium in the conditioning chamber (5.3.2) shall be

(50 ± 0,5) mm × (25 ± 0,5) mm × (15 ± 0,5) mm.

For the purposes of calculating the mass of preservative retained per unit volume of wood (8.1.2.2) the

nominal volume of each test specimen shall be taken as 18,75 cm .
Mark each test specimen so that it can be identified throughout the test.
7.5 Number of test specimens
Use:
a) five test specimens (see 7.4) for each preservative and each concentration;

2) The growth of young larvae of Anobium punctatum is slow in test specimens from resinous wood. Results from test

specimens in resinous wood should be compared with those obtained from oak test specimens.

3) It is not essential in this test for the starch content to be high.

4) These test specimens may be taken from the trunk of the tree or the large branches.

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SIST EN 49-2:2015
EN 49-2:2015 (E)

b) five untreated control test specimens (see 7.4) for a complete test of any given preservative;

c) five control test specimens (7.4) treated with that solvent or diluent (5.2.4 or 5.2.5) if a solvent or diluent

(water included) is used.

It is advisable to treat more than the specified number of test specimens so that, after weighing, any test

specimens with abnormally high or low retentions can be rejected from the batch.
8 Procedure
8.1 Preparation of the test specimens
8.1.1 Conditioning of test specimens before treatment

Allow the test specimens to condition in the conditioning chamber (5.3.2), for a minimum of two weeks.

8.1.2 Treatment of the test specimens
8.1.2.1 Preparation of the treatment solution
8.1.2.1.1 Solid preservatives
Water-soluble preservatives:

— dissolve the preservative in the water (5.2.4) to the required concentration, or to a series of

concentrations if toxic values are to be determined.
Non-water-soluble preservatives:

— dissolve the preservative in an appropriate solvent (5.2.5) to the required concentration, or to a series of

concentrations if toxic values are to be determined.
8.1.2.1.2 Liquid preservatives

If appropriate, use the preservative without further preparation other than any necessary stirring. If it is a

concentrate or if toxic values are to be determined, dilute the preservative with the diluent to the required

working concentration, using the procedure specified by the manufacturer.
All treatment solutions shall be freshly prepared.
8.1.2.1.3 Toxic values

If toxic values are to be determined, prepare a series of at least five concentrations by mass, distributed

evenly about the expected toxic values. A solvent or diluent control, i.e. treatment at concentration = 0, shall

also be used. If the approximate toxic values are unknown, the concentrations shall form a widely spaced

geometric progression for a first test and a more closely spaced geometric or arithmetic progression for

subsequent tests.
All treatment solutions shall be freshly prepared.
8.1.2.2 Impregnation

Carry out impregnation in ascending order of concentration, starting with the solvent control

(concentration = 0).
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SIST EN 49-2:2015
EN 49-2:2015 (E)

The following procedure ensures the required complete impregnation of test specimens by the test solutions.

For each concentration weigh each test specimen, to the nearest 0,05 g, and then stack the test specimens in

one of the treatment vessels (5.3.3) so that as much of their face as possible is exposed (e.g. by piling them

crosswise). Ballast the stack of test specimens with the weights (5.3.9) to prevent them floating later when the

liquid is admitted.

Place each vessel in one of the vacuum vessels (5.3.7), attach the vacuum pump (5.3.8) and reduce the

pressure to 700 Pa. Maintain this vacuum for 15 min. Observe the proper safety measures for vacuum

vessels. After this period, close the stopcock to the vacuum pump (5.3.8) and open the other stopcock to allow

the solution of preservative to be drawn into the treatment vessel. Keep the test specimens covered

completely by the solution throughout the remainder of the impregnation process.

Next, admit air to bring the vacuum vessel back to atmospheric pressure, remove the treatment vessel with its

submerged test specimens from the vacuum vessel, cover it and leave it for 2 h, adding further solution as

necessary to keep the test specimens fully covered by liquid.

After this impregnation treatment, remove the test specimens one by one, remove the excess liquid from their

surfaces by lightly blotting with filter paper (5.2.6) and immediately weigh each to the nearest 0,05 g.

In the case of water-soluble preservatives, for example salts and organic chemicals which are being studied

as active substances, calculate the mass of active matter retained by each test specimen from the mass of

solution absorbed and its concentration .

In the case of organic formulations and organic water-dispersible formulations the retention is expressed for

each test specimen in terms of the corresponding mass of the formulation retained but, if a concentrate is

supplied, the retention is expressed in terms of the solution prepared ready for use as specified by the

manufacturer.

Calculate the mass of preservative retained per unit volume of wood in kilograms per cubic metre, for each

test specimen.

Calculate the mean mass of preservative retained per unit volume of wood for each set of five test specimens.

8.1.3 Drying and conditioning of the test specimens after treatment

Arrange the impregnated test specimens treated with each preservative concentration on their narrow faces,

resting on two glass rods, not touching each other in the drying vessel (5.3.6). Place the cover on the drying

vessel. Place the drying vessel in the conditioning chamber (5.3.2). Invert the test specimens twice each week

during the subsequent drying period, temporarily removing the cover to perform these operations.

To prevent mould growth on test specimens treated with water-diluted preservatives, place a small dish

containing the xylene (5.2.3) in the drying vessel (5.3.6).
During the first week retain the cover on the drying vessel.
During the second week uncover the drying vessel progressively each day.

From the beginning of the third week leave the drying vessel fully open. Drying shall be complete at the end of

the fourth week.

NOTE The drying and conditioning of the test specimens depend on the nature of the product under test and on the

solvent or diluent used. For slow drying products, it may be necessary to extend the conditioning process.

5) When dealing with preservative formulations whose constituents may be selectively absorbed by wood, it is necessary

to carry out chemical analysis of the solution before and after impregnation. Similarly, analysis is recommended if very

dilute solutions are used.
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SIST EN 49-2:2015
EN 49-2:2015 (E)

If in the case of slow drying products, the conditioning period is extended, the extended conditioning period

shall be stated in the test report.

If the test specimens are to be subject to an ageing procedure, this shall be carried out after this drying

procedure.
8.2 Exposure of the test specimens to the insects

Coat the transverse faces of each test specimen with the paraffin wax (5.2.1) applied as a single brush coat at

70 °C to 90 °C, and allow to dry for 24 h.

Next prepare the egg-laying zones by attaching a piece of the fine cloth (5.2.7) measuring approximately

45 mm × 20 mm on each large face of the test specimen. Use the paste (5.2.2) to attach the cloth and smooth

this out so that the mash openings are not twisted.

Immediately prior to exposure to egg-laying, condition all the test specimens for one week in the testing

chamber (5.3.5).

Place each test specimen in one of the test containers (5.3.11) and add five female insects and at least five

male insects. Cover the container with a disc of filter paper (5.2.6). Keep this
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