Foodstuffs - Determination of aflatoxin B1, and the total content of aflatoxins B1, B2, G1 and G2 in cereals, nuts and derived products - High-performance liquid chromatographic method (ISO 16050:2003)

ISO 16050:2003 specifies a reverse-phase high-performance liquid chromatographic method, with immunoaffinity column clean-up and post-column derivatization, for the determination of aflatoxins in cereals, nuts and derived products. The limit of quantification for aflatoxin B1, and for the sum of aflatoxins B1, B2, G1 and G2, is 8 micrograms per kilogram.
The method has been validated for maize containing 24,5 micrograms per kilogram, for peanut butter containing 8,4 micrograms per kilogram, and for raw peanuts containing 16 micrograms per kilogram of total aflatoxins. It has also been shown that this method can be used for oilseed products, dried fruits and derived products.

Lebensmittel - Bestimmung von Aflatoxin B1 und der Summe von Aflatoxin B1, B2, G1 und G2 in Getreiden, Nüssen und verwandten Produkten - Hochleistungsflüssigchromatographisches Verfahren (ISO 16050:2003)

Diese Internationale Norm legt ein Verfahren zur Bestimmung von Aflatoxinen in Getreiden, Nüssen und verwandten Produkten fest, wobei die Umkehrphasen Hochleistungsflüssigchromatographie mit Reinigung an einer Immunoaffinitätssäule und Nachsäulenderivatisierung angewandt wird. Die Bestimmungsgrenze für Aflatoxin B1 und für die Summe von Aflatoxin B1, B2, G1 und G2 beträgt 8 µg/kg.
Das Verfahren wurde für Mais mit 24,5 μg/kg, Erdnusscreme mit 8,4 μg/kg und unbehandelte Erdnüsse mit 16 μg/kg Gesamtaflatoxingehalt validiert. Außerdem wurde gezeigt, dass dieses Verfahren auf Ölsaaten, Trockenobst und verwandte Produkte angewendet werden kann.

Produits alimentaires - Dosage de l'aflatoxine B1 et détermination de la teneur totale en aflatoxines B1, B2, G1 et G2 dans les céréales, les fruits à coque et les produits dérivés - Méthode par chromatographie liquide à haute performance (ISO 16050:2003)

L'ISO 16050:2003 spécifie une méthode par chromatographie liquide à haute performance en phase inversée, avec purification sur colonne d'immunoaffinité et dérivation post-colonne, pour le dosage des aflatoxines dans les céréales, les fruits à coque et les produits dérivés. La limite de quantification de l'aflatoxine B1 et de la somme des aflatoxines B1, B2, G1 et G2 est de 8 µg/kg.
Cette méthode a été validée sur du maïs, du beurre d'arachide et des arachides brutes ayant respectivement une teneur en aflatoxines totales de 24,5 µg/kg, 8,4 µg/kg et 16 µg/kg. Il a également été démontré que la présente méthode peut être utilisée pour les produits oléagineux, les fruits secs et les produits dérivés.

Živila - Določevanje aflatoksina B1 in vsote aflatoksinov B1, B2, G1 in G2 v žitih in oreščkih ter njihovih proizvodih - Metoda tekočinske kromatografije visoke ločljivosti (ISO 16050:2003)

Ta mednarodni standard opredeljuje metodo tekočinske kromatografije visoke ločljivosti z reverzno fazo, z imunoafinitetnim kolonskim čiščenjem in derivacijo za kolono, za določevanje aflatoksinov v žitih in oreščkih ter njihovih proizvodih. Meja kvantifikacije za aflatoksin B1 in za vsoto aflatoksinov B1, B2, G1 in G2, je 8 μg/kg. Metoda je bila potrjena za koruzo, ki vsebuje 24,5 μg/kg, za arašidovo maslo, ki vsebuje 8,4 μg/kg, in za surove arašide, ki vsebujejo 16 μg/kg celotnih aflatoksinov. Prav tako je bilo prikazano, da se ta metoda lahko uporablja za proizvode iz oljnic in sušeno sadje ter njihove proizvode.

General Information

Status
Published
Publication Date
12-Jul-2011
Withdrawal Date
30-Jan-2012
Current Stage
6060 - Definitive text made available (DAV) - Publishing
Start Date
13-Jul-2011
Due Date
29-May-2012
Completion Date
13-Jul-2011

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SLOVENSKI STANDARD
SIST EN ISO 16050:2011
01-oktober-2011
1DGRPHãþD
SIST EN 12955:2000
äLYLOD'RORþHYDQMHDIODWRNVLQD%LQYVRWHDIODWRNVLQRY%%*LQ*YåLWLKLQ
RUHãþNLKWHUQMLKRYLKSURL]YRGLK0HWRGDWHNRþLQVNHNURPDWRJUDILMHYLVRNH
ORþOMLYRVWL ,62
Foodstuffs - Determination of aflatoxin B1, and the total content of aflatoxins B1, B2, G1
and G2 in cereals, nuts and derived products - High-performance liquid chromatographic
method (ISO 16050:2003)
Lebensmittel - Bestimmung von Aflatoxin B1 und der Summe von Aflatoxin B1, B2, G1
und G2 in Getreiden, Nüssen und verwandten Produkten -
Hochleistungsflüssigchromatographisches Verfahren (ISO 16050:2003)
Produits alimentaires - Dosage de l'aflatoxine B1 et détermination de la teneur totale en
aflatoxines B1, B2, G1 et G2 dans les céréales, les fruits à coque et les produits dérivés
- Méthode par chromatographie liquide à haute performance (ISO 16050:2003)
Ta slovenski standard je istoveten z: EN ISO 16050:2011
ICS:
67.050 Splošne preskusne in General methods of tests and
analizne metode za živilske analysis for food products
proizvode
67.060 äLWDVWURþQLFHLQSURL]YRGLL] Cereals, pulses and derived
QMLK products
67.080.10 Sadje in sadni proizvodi Fruits and derived products
SIST EN ISO 16050:2011 en
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN ISO 16050:2011

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SIST EN ISO 16050:2011


EUROPEAN STANDARD
EN ISO 16050

NORME EUROPÉENNE

EUROPÄISCHE NORM
July 2011
ICS 67.060; 67.080.10 Supersedes EN 12955:1999
English Version
Foodstuffs - Determination of aflatoxin B1, and the total content
of aflatoxins B1, B2, G1 and G2 in cereals, nuts and derived
products - High-performance liquid chromatographic method
(ISO 16050:2003)
Produits alimentaires - Dosage de l'aflatoxine B1 et Lebensmittel - Bestimmung von Aflatoxin B1 und der
détermination de la teneur totale en aflatoxines B1, B2, G1 Summe von Aflatoxin B1, B2, G1 und G2 in Getreiden,
et G2 dans les céréales, les fruits à coque et les produits Nüssen und verwandten Produkten -
dérivés - Méthode par chromatographie liquide à haute Hochleistungsflüssigchromatographisches Verfahren (ISO
performance (ISO 16050:2003) 16050:2003)
This European Standard was approved by CEN on 17 June 2011.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same
status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland,
Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.





EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2011 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 16050:2011: E
worldwide for CEN national Members.

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SIST EN ISO 16050:2011
EN ISO 16050:2011 (E)
Contents Page
Foreword .3

2

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SIST EN ISO 16050:2011
EN ISO 16050:2011 (E)
Foreword
The text of ISO 16050:2003 has been prepared by Technical Committee ISO/TC 34 “Food products” of the
International Organization for Standardization (ISO) and has been taken over as EN ISO 16050:2011 by
Technical Committee CEN/TC 275 “Food analysis - Horizontal methods” the secretariat of which is held by
DIN.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by January 2012, and conflicting national standards shall be withdrawn at
the latest by January 2012.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such
...

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