EN 46-1:2005
(Main)Wood preservatives - Determination of the preventive action against Hylotrupes bajulus (Linnaeus) - Part 1: Larvicidial effect (Laboratory method)
Wood preservatives - Determination of the preventive action against Hylotrupes bajulus (Linnaeus) - Part 1: Larvicidial effect (Laboratory method)
This document specifies a method for the determination of the preventive action of a wood preservative against recently hatched larvae of Hylotrupes bajulus (Linnaeus) when the preservative is applied as a surface treatment to wood.
This method is applicable to:
¾ water-insoluble chemicals which are being studied as active insecticides,
¾ organic formulations, as supplied or as prepared in the laboratory by dilution of concentrates,
¾ organic water-dispersible formulations as supplied or as prepared in the laboratory by dilution of concentrates, and
¾ water-soluble materials, for example salts.
The method is applicable whether or not the test specimens have been subjected to appropriate ageing procedures.
Holzschutzmittel - Bestimmung der vorbeugenden Wirkung gegenüber Hylotrupes bajulus (Linnaeus) - Teil 1: Larvizide Wirkung (Laboratoriumsverfahren)
Dieses Dokument legt ein Verfahren zur Bestimmung der vorbeugenden Wirkung eines Holzschutzmittels gegenüber Eilarven von Hylotrupes bajulus (Linnaeus) fest, wobei das Holzschutzmittel zur Oberflächenbehandlung des Holzes verwendet wird.
Das Verfahren gilt für:
- wasserunlösliche Chemikalien, die als wirksame Insektizide untersucht werden;
- organische Formulierungen im Anlieferungszustand oder in Form von im Laboratorium hergestellten Verdünnungen von Konzentraten;
- organische, in Wasser dispergierbare Formulierungen im Anlieferungszustand oder in Form von im Laboratorium hergestellten Verdünnungen von Konzentraten; und
- wasserlösliche Stoffe, zum Beispiel Salze.
Das Verfahren gilt unabhängig davon, ob die Prüfkörper einer angemessenen Alterungsbeanspruchung ausgesetzt wurden oder nicht.
Produits de préservation du bois - Détermination de l'action préventive contre Hylotrupes bajulus (Linnaeus) - Partie 1: Effet larvicide (Méthode de laboratoire)
Le présent document prescrit une méthode de détermination de l'action préventive d'un produit de préservation du bois contre les larves récemment écloses d'Hylotrupes bajulus (Linnaeus) lorsque le produit est appliqué au bois par un traitement de surface.
La présente méthode est applicable :
- aux produits chimiques non hydrosolubles étudiés en tant que matières actives insecticides ;
- aux formules organiques telles qu'elles sont fournies ou préparées au laboratoire par dilution de concentrés ;
- aux formulations organiques hydrodispersables telles qu'elles sont fournies ou préparées au laboratoire par dilution de concentrés, et
- aux produits solubles dans l'eau, par exemple aux sels.
Cette méthode s'applique à des éprouvettes d'essai ayant subi ou non des épreuves de vieillissement appropriées.
Zaščitna sredstva za les - Ugotavljanje preventivnega delovanja proti hišnemu kozličku Hylotrupes bajulus (Linnaeus) – 1. del.: Larvicidial effect (Laboratorijska metoda)
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
01-julij-2005
1DGRPHãþD
SIST EN 46:1995
=DãþLWQDVUHGVWYD]DOHV8JRWDYOMDQMHSUHYHQWLYQHJDGHORYDQMDSURWLKLãQHPX
NR]OLþNX+\ORWUXSHVEDMXOXV /LQQDHXV ±GHO /DUYLFLGLDOHIIHFW /DERUDWRULMVND
PHWRGD
Wood preservatives - Determination of the preventive action against Hylotrupes bajulus
(Linnaeus) - Part 1: Larvicidial effect (Laboratory method)
Holzschutzmittel - Bestimmung der vorbeugenden Wirkung gegenüber Hylotrupes
bajulus (Linnaeus) - Teil 1: Larvizide Wirkung (Laboratoriumsverfahren)
Produits de préservation du bois - Détermination de l'action préventive contre Hylotrupes
bajulus (Linnaeus) - Partie 1: Effet larvicide (Méthode de laboratoire)
Ta slovenski standard je istoveten z: EN 46-1:2005
ICS:
71.100.50 .HPLNDOLMH]D]DãþLWROHVD Wood-protecting chemicals
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EUROPEAN STANDARD
EN 46-1
NORME EUROPÉENNE
EUROPÄISCHE NORM
March 2005
ICS 71.100.50 Supersedes EN 46:1988
English version
Wood preservatives - Determination of the preventive action
against Hylotrupes bajulus (Linnaeus) - Part 1: Larvicidial effect
(Laboratory method)
Produits de préservation des bois - Détermination de Holzschutzmittel - Bestimmung der vorbeugenden Wirkung
l'action préventive contre Hylotrupes bajulus (Linnaeus) - gegenüber Hylotrupes bajulus (Linnaeus) - Teil 1: Larvizide
Partie 1: Effet larviciel (Méthode de laboratoire) Wirkung (Laboratoriumsverfahren)
This European Standard was approved by CEN on 3 February 2005.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the Central Secretariat or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the Central Secretariat has the same status as the official
versions.
CEN members are the national standards bodies of Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France,
Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Slovakia,
Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36 B-1050 Brussels
© 2005 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 46-1:2005: E
worldwide for CEN national Members.
Contents Page
Foreword.3
Introduction .4
1 Scope .5
2 Normative reference .5
3 Terms and definitions .5
4 Principle.6
5 Test materials.6
6 Sampling.7
7 Test specimens.7
8 Procedure .8
9 Expression of results .12
10 Test report .13
Annex A (informative) Example of a test report .15
Annex B (informative) Technique for culturing Hylotrupes bajulus (Linnaeus) .17
Annex C (informative) Differentiation of heartwood and sapwood in Pinus species.20
Annex D (normative) Method for treating test specimens by pipette .21
Annex E (informative) Environmental, health and safety precautions within chemical/biological
laboratory .23
Bibliography .24
Foreword
This document (EN 46-1:2005) has been prepared by Technical Committee CEN/TC 38 “Durability of wood
and wood-based products”, the secretariat of which is held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by September 2005, and conflicting national standards shall be
withdrawn at the latest by September 2005.
This document supersedes EN 46:1988.
This document consist of two parts to enable preventive action of wood preservatives ,against recently
hatched larvae of Hylotrupes bajulus , which are intended to be applied by surface treatment; Part 1 is
required to determine the larvicidial effect of preservatives and Part 2 is required to determine the ovicidal
action of the preservatives after egg-laying of young females.
Significant technical differences between this document and EN 46:1988 are as follows:
a) introduction of new harmonised specifications for the test specimens used in the diverse biological tests;
b) acknowledgement of the terms given in EN 1001-1;
c) introduction of an informative Annex to take account of consideration for minimisation of environmental
and health hazards caused by the use of this biological test.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Cyprus, Czech Republic,
Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Slovakia, Slovenia, Spain, Sweden, Switzerland
and United Kingdom.
Introduction
This document describes a laboratory method of testing which gives a basis for the assessment of the
preventive action of a wood preservative, when applied as a surface treatment, against recently hatched
larvae of Hylotrupes bajulus, whereas the method for determining the toxic values against Hylotrupes bajulus
(EN 47) provides a means of checking whether a preservative prevents attack by these larvae and prevents
their survival within totally impregnated wood.
This method makes it possible to determine whether recently hatched larvae are capable of boring through the
treated surface of a susceptible wood species and of surviving in the untreated part of the test specimen. For
this purpose, the procedure seeks to reproduce normal egg-laying conditions existing in cracks in wood, which
provide the principal egg-laying sites. It takes account of the fact that, if larvae pass through the treated
surface, they will then tunnel in the direction of the least protected regions of the wood.
This laboratory method provides one criterion by which the value of a preservative can be assessed. In
making this assessment, the methods by which the preservative may be applied should be taken into account.
This test is of particular interest when applied to test specimens which have been subjected to an ageing
procedure. It is further recommended that results from this test should be supplemented by those from other
appropriate tests and, above all, by practical experience.
When products which are very active at low concentrations are used it is very important to take suitable
precautions to isolate and separate, as far as possible, operations involving chemical products, other products,
treated wood, laboratory apparatus and clothing. Suitable precautions should include the use of separate
rooms, areas within rooms, extraction facilities, conditioning chambers and special training for personnel (see
also Annex E for environmental, health and safety precautions).
1 Scope
This document specifies a method for the determination of the preventive action of a wood preservative
against recently hatched larvae of Hylotrupes bajulus (Linnaeus) when the preservative is applied as a surface
treatment to wood.
This method is applicable to:
water-insoluble chemicals which are being studied as active insecticides;
organic formulations, as supplied or as prepared in the laboratory by dilution of concentrates;
organic water-dispersible formulations as supplied or as prepared in the laboratory by dilution of
concentrates, and
water-soluble materials, for example salts.
The method is applicable whether or not the test specimens have been subjected to appropriate ageing
procedures.
2 Normative reference
The following referenced documents are indispensable for the application of this document. For dated
references, only the edition cited applies. For undated references, the latest edition of the referenced
document (including any amendments) applies.
EN ISO 3696, Water for analytical laboratory use – Specification and test methods (ISO 3696:1987)
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1
representative sample
sample having its physical or chemical characteristics identical to the volumetric average characteristics of the
total volume being sampled
3.2
supplier
sponsor of the test (person or company providing the sample of wood preservative to be tested)
4 Principle
Depending on the test being carried out either
on a set of test specimens of a susceptible wood species that is surface treated with a solution of the
preservative, or
if toxic values are to be determined, on several sets of test specimens of a susceptible wood species that
are surface treated with a series of solutions in which the concentration of preservative is ranged in a
given progression.
The treated test specimens are exposed to recently hatched larvae of Hylotrupes bajulus. The resulting attack
is observed and compared with those in untreated control test specimens. If the preservative has been
prepared in the laboratory by dilution of a concentrate or by dissolution of a solid, the resulting attack is also
compared to that in solvent or diluent treated control test specimens.
5 Test materials
5.1 Biological material
5.1.1 Hylotrupes bajulus (Linnaeus) larvae, within three days of hatching.
5.1.2 Source of larvae. Obtain the larvae from cultures reared e.g. as described in Annex B.
5.1.3 Provision of larvae. Collect larvae from eggs laid by different females.
5.1.4 Choice of larvae. Use a mixed batch of these larvae for the test. Use 10 larvae per treated test
specimen or control test specimen.
5.2 Products and reagents
5.2.1 Paraffin wax, for fixing the glass plate and for sealing the end faces of test specimens to be treated
with solutions in all cases in which water is the continuous phase.
NOTE Paraffin wax with a setting point of 52 °C to 54 °C has been found to be suitable.
5.2.2 Gelatin, for sealing the end faces of test specimens to be treated with solutions in which an organic
solvent is the continuous phase.
5.2.3 Water, complying with grade 3 of EN ISO 3696.
5.2.4 Solvent or diluent, a volatile liquid that will dissolve or dilute the preservative but does not leave a
residue in the wood at the end of the post-treatment conditioning period that has a toxic effect on the insects.
CAUTION — Do not use benzene or other solvents which pose a health risk.
5.3 Apparatus
5.3.1 Culturing chamber, with air circulation, and controlled at (28 ± 2) °C and at a relative humidity of
(70 ± 5) %.
5.3.2 Conditioning chamber, well ventilated and controlled at (20 ± 2) °C and at a relative humidity of
(65 ± 5) %.
NOTE The conditioning of test specimens may be carried out in the laboratory work area (see 5.3.3) provided that
this has the conditions specified for the conditioning chamber (see 5.3.2).
5.3.3 Laboratory work area, well ventilated, where treatment of the test specimens is carried out.
CAUTION — It is essential to follow safety procedures for handling flammable and toxic materials.
Avoid excessive exposure of operators to solvents or their vapours.
5.3.4 Testing chamber, ventilated and air conditioned, controlled at (22 ± 2) °C and at a relative humidity
of (70 ± 5) %.
5.3.5 Treatment vessels, of a material that does not react with the preservative under test, for example of
glass for organic products and of polyethylene for salts containing fluorine.
5.3.6 Weights, to provide ballast for the test specimens. The weights shall not react with any materials with
which they come into contact during the test.
5.3.7 Safety equipment and protective clothing, appropriate for the test product and the test solvent, to
ensure the safety of the operator.
5.3.8 Glass plates, 48 mm long and 25 mm wide, intended to provide a lateral s
...
Questions, Comments and Discussion
Ask us and Technical Secretary will try to provide an answer. You can facilitate discussion about the standard in here.