Food authenticity - Determination of the sum of 16-O-Methylcafestol, 16-O-Methylkahweol and their derivatives in roasted coffee by ¹H-qNMR

This document specifies a method for the determination of soluble 16-O-Methylcafestol and 16-O-Methylkahweol content (the sum of free forms and derivatives, e.g. fatty acid esters, henceforth abbreviated as 16-OMD = “diterpenes”) in roasted coffee (beans or ground), using quantitative proton nuclear magnetic resonance spectroscopy (1H-qNMR).
If complying with the experimental parameters described below, this test procedure has been proven for the following mass fraction range:
w16-OMD:    20 mg/kg to 2 000 mg/kg.
The mass fraction range can be expanded by suitable changes of the experimental parameters, e.g. a different weighed portion of ground coffee or the accumulation of more NMR-transients.

Lebensmittelauthentizität - Bestimmung des Gehalts von 16-O-Methylcafestol, 16-O-Methylkahweol und deren Derivaten als Summenparameter in Röstkaffee mittels ¹H-qNMR

Dieses Dokument legt ein Verfahren zur Bestimmung des Gehalts an löslichem 16 O Methylcafestol und 16 O Methylkahweol (Summe der freien Formen und Derivate, z. B. Fettsäureester, im Folgenden abgekürzt als 16 OMD = „Diterpene“) in geröstetem Kaffee (Bohnen oder gemahlen) mittels quantitativer Protonen-nuklearmagnetischer Resonanzspektroskopie (1H qNMR) fest.
Bei Einhaltung der nachstehend beschriebenen Versuchsparameter hat sich dieses Prüfverfahren für den folgenden Bereich der Massenanteile bewährt:
w16-OMD:   20 mg/kg bis 2 000 mg/kg.
Der Bereich der Massenanteile kann durch geeignete Änderungen der Versuchsparameter erweitert werden, z. B. durch eine andere Einwaage des gemahlenen Kaffees oder die Akkumulation von mehr NMR Transienten.

Authenticité des aliments - Détermination par résonance magnétique nucléaire quantitative du proton (RMN-¹H) de la somme des teneurs en 16-O-méthylcafestol, 16-O-méthylkahwéol et leurs dérivés dans le café torréfié

Le présent document spécifie une méthode de détermination de la teneur en 16-O-méthylcafestol et 16 O-méthylkahwéol solubles (somme des formes libres et des dérivés, par exemple esters d’acides gras, désormais abrégé 16-OMD = « diterpènes ») du café torréfié (en grains ou moulu), par spectroscopie par résonance magnétique nucléaire quantitative du proton (qRMN-1H).
En respectant les paramètres expérimentaux décrits ci-dessous, ce mode opératoire d’essai a été validé pour la gamme de fractions massiques suivantes :
w16-OMD :   20 mg/kg à 2 000 mg/kg.
La gamme de fractions massiques peut être élargie en modifiant adéquatement les paramètres expérimentaux, par exemple une prise d’essai différente de café moulu ou l’accumulation de plusieurs transitions RMN.

Pristnost živil - Določanje vsote 16-O-metilkafestola, 16-O-metilkaveola in njunih derivatov v praženi kavi z metodo ¹H-qNMR

General Information

Status
Not Published
Publication Date
25-Sep-2024
Current Stage
5020 - Submission to Vote - Formal Approval
Start Date
06-Jun-2024
Due Date
22-Jul-2024
Completion Date
06-Jun-2024

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Standards Content (Sample)

SLOVENSKI STANDARD
oSIST prEN 17992:2023
01-september-2023
Pristnost živil - Določanje vsote 16-O-metilkafestola, 16-O-metilkaveola in njunih
derivatov v praženi kavi z metodo ¹H-qNMR
Food authenticity - Determination of the sum of 16-O-methylcafestol, 16-O-
Methylkahweol and their derivatives in roasted coffee by ¹H-qNMR
Lebensmittelauthentizität - Bestimmung des Gehalts von 16-O-Methylcafestol, 16-O-
Methylkahweol und deren Derivaten als Summenparameter in Röstkaffee mittels ¹H-
qNMR
Authenticité alimentaire du café - Détermination de la teneur en 16-O-méthylcafestol -
Méthode par NMR
Ta slovenski standard je istoveten z: prEN 17992
ICS:
67.140.20 Kava in kavni nadomestki Coffee and coffee substitutes
oSIST prEN 17992:2023 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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oSIST prEN 17992:2023

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oSIST prEN 17992:2023
DRAFT
EUROPEAN STANDARD
prEN 17992
NORME EUROPÉENNE
EUROPÄISCHE NORM
June 2023
ICS 67.140
English Version
Food authenticity - Determination of the sum of 16-O-
Methylcafestol, 16-O-Methylkahweol and their
1
derivatives in roasted coffee by H-qNM
Authenticité alimentaire du café - Détermination de la Lebensmittelauthentizität - Bestimmung des Gehalts
teneur en 16-O-méthylcafestol - Méthode par NMR von 16-O-Methylcafestol, 16-O-Methylkahweol und
deren Derivaten als Summenparameter in Röstkaffee
1
mittels H-qNMR
This draft European Standard is submitted to CEN members for enquiry. It has been drawn up by the Technical Committee
CEN/TC 460.
If this draft becomes a European Standard, CEN members are bound to comply with the CEN/CENELEC Internal Regulations
which stipulate the conditions for giving this European Standard the status of a national standard without any alteration.
This draft European Standard was established by CEN in three official versions (English, French, German). A version in any other
language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC
Management Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
Recipients of this draft are invited to submit, with their comments, notification of any relevant patent rights of which they are
aware and to provide supporting documentation.
Warning : This document is not a European Standard. It is distributed for review and comments. It is subject to change without
notice and shall not be referred to as a European Standard.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2023 CEN All rights of exploitation in any form and by any means reserved Ref. No. prEN 17992:2023 E
worldwide for CEN national Members.

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oSIST prEN 17992:2023
prEN 17992:2023 (E)
Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative references . 5
3 Terms and definitions . 5
4 Reagents . 8
5 Apparatus . 9
6 Measurement and test methods . 10
6.1 General. 10
6.2 Sampling .
...

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