EN ISO 21479:2020

Soil quality - Determination of the effects of pollutants on soil flora - Leaf fatty acid composition of plants to assess soil quality (ISO 21479:2019)

This document describes a method to compare the quality of soils by determining the fatty acid composition of the leaves of plant species grown in these soils.
This method does not make it possible to determine an optimal value of the Omega-3 index and, therefore, cannot be used to determine the intrinsic quality of a soil from a specific area (regarded as homogeneous). The method can only be used to compare the quality of soils between various areas.
This method is applicable to:
—          soils from contaminated sites;
—          amended soils;
—          soils after remediation;
?      soil with waste products (e.g. slurry, manure, sludge or composts).
Alternatively, the quality of soils can be assessed by determining the Omega-3 index of Lactuca sativa seedlings grown in these soils under controlled conditions (i.e. phytotronic chamber) and by comparing these values to those obtained from control soils (see Annex B).

Bodenbeschaffenheit - Bestimmung der Wirkungen von Schadstoffen auf die Bodenflora - Fettsäurezusammensetzung in Blättern zur Beurteilung der Bodenbeschaffenheit (ISO 21479:2019)

Gegenstand dieses Dokuments ist ein Verfahren zum Vergleichen der Beschaffenheit von Böden durch Bestimmen der Fettsäurezusammensetzung in den Blättern von Pflanzen, die auf diesen Böden wachsen.
Dieses Verfahren ermöglicht keine Bestimmung des optimalen Werts des Omega 3 Index. Daher kann es nicht dazu verwendet werden, die intrinsische Qualität des Bodens auf einer bestimmten (als homogen angesehenen) Fläche zu bestimmen. Das Verfahren kann nur dazu verwendet werden, die Beschaffenheit von Böden auf verschiedenen Flächen miteinander zu vergleichen.
Das Verfahren ist anwendbar auf:
   Böden von kontaminierten Standorten;
   verbesserte Böden;
   Böden nach Altlastensanierungen;
   Böden, auf die Abfallprodukte (z. B. Flüssigmist, Gülle, Schlamm oder Kompost) aufgebracht wurden.
Alternativ kann die Beschaffenheit von Böden beurteilt werden, indem der Omega 3 Index von Lactuca sativa Keimlingen bestimmt wird, die in diesen Böden unter kontrollierten Bedingungen (d. h. in einer Wachstumskammer) gezogen werden, und diese Werte mit den bei Kontrollböden erhaltenen Werten verglichen werden (siehe Anhang B).

Qualité du sol - Détermination des effets des polluants sur la flore du sol - Composition en acides gras foliaires des plantes utilisées pour évaluer la qualité du sol (ISO 21479:2019)

Le présent document décrit une méthode visant à comparer la qualité des sols en déterminant la composition en acides gras des feuilles d'espèces végétales poussant sur ces sols.
Cette méthode ne permet pas de déterminer une valeur optimale de l'indice Oméga-3 et ne peut donc pas être utilisée pour déterminer la qualité intrinsèque d'un sol d'une zone spécifique (considérée homogène). La méthode peut être utilisée uniquement pour comparer la qualité des sols entre plusieurs zones.
Cette méthode est applicable à:
—          des sols provenant de sites contaminés;
—          des sols amendés;
—          des sols après remédiation;
—          des sols contenant des produits résiduaires (par exemple lisier, fumier, boues ou composts).
La qualité des sols peut aussi être évaluée en déterminant l'indice Oméga-3 de plantules de Lactuca sativa poussant dans ces sols dans des conditions contrôlées (c'est-à-dire, enceinte phytotronique) et en comparant ces valeurs avec celles obtenues à partir de sols témoins (voir l'Annexe B).

Kakovost tal - Določanje učinkov onesnaževal na floro tal - Sestava maščobnih kislin v listih rastlin za oceno kakovosti tal (ISO 21479:2019)

General Information

• Status: Published
• Publication Date: 05-May-2020
• Withdrawal Date: 29-Nov-2020
• ICS: 13.080.30 - Biological properties of soils
• Technical Committee: CEN/TC 444 - Environmental characterization
• Drafting Committee: CEN/TC 444 - Environmental characterization
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 06-May-2020
• Due Date: 07-Mar-2022
• Completion Date: 06-May-2020
• Ref Project: SIST EN ISO 21479:2020 - Soil quality - Determination of the effects of pollutants on soil flora - Leaf fatty acid composition of plants to assess soil quality (ISO 21479:2019)

Standards Content (Sample)

SLOVENSKI STANDARD
01-november-2020
Kakovost tal - Določanje učinkov onesnaževal na floro tal - Sestava maščobnih
kislin v listih rastlin za oceno kakovosti tal (ISO 21479:2019)
Soil quality - Determination of the effects of pollutants on soil flora - Leaf fatty acid
composition of plants to assess soil quality (ISO 21479:2019)
Bodenbeschaffenheit - Bestimmung der Wirkungen von Schadstoffen auf die Bodenflora
- Fettsäurezusammensetzung in Blättern zur Beurteilung der Bodenbeschaffenheit (ISO
21479:2019)
Qualité du sol - Détermination des effets des polluants sur la flore du sol - Composition
en acides gras foliaires des plantes utilisées pour évaluer la qualité du sol (ISO
21479:2019)
Ta slovenski standard je istoveten z: EN ISO 21479:2020
ICS:
13.080.30 Biološke lastnosti tal Biological properties of soils
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 21479
EUROPEAN STANDARD
NORME EUROPÉENNE
May 2020
EUROPÄISCHE NORM
ICS 13.080.30
English Version
Soil quality - Determination of the effects of pollutants on
soil flora - Leaf fatty acid composition of plants to assess
soil quality (ISO 21479:2019)
Qualité du sol - Détermination des effets des polluants Bodenbeschaffenheit - Bestimmung der Wirkungen
sur la flore du sol - Composition en acides gras foliaires von Schadstoffen auf die Bodenflora -
des plantes utilisées pour évaluer la qualité du sol (ISO Zusammensetzung von Fettsäuren in Blättern zur
21479:2019) Beurteilung der Bodenbeschaffenheit (ISO
21479:2019)
This European Standard was approved by CEN on 13 April 2020.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2020 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 21479:2020 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
The text of ISO 21479:2019 has been prepared by Technical Committee ISO/TC 190 "Soil Quality” of the
International Organization for Standardization (ISO) and has been taken over as EN ISO 21479:2020 by
Technical Committee CEN/TC 444 “Environmental characterization of solid matrices” the secretariat of
which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by November 2020, and conflicting national standards
shall be withdrawn at the latest by November 2020.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the
United Kingdom.
Endorsement notice
The text of ISO 21479:2019 has been approved by CEN as EN ISO 21479:2020 without any modification.

INTERNATIONAL ISO
STANDARD 21479
First edition
2019-06
Soil quality — Determination of the
effects of pollutants on soil flora —
Leaf fatty acid composition of plants
used to assess soil quality
Qualité du sol — Détermination des effets des polluants sur la flore du
sol — Composition en acides gras foliaires des plantes utilisées pour
évaluer la qualité du sol
Reference number
ISO 21479:2019(E)
©
ISO 2019
ISO 21479:2019(E)
© ISO 2019
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting
on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address
below or ISO’s member body in the country of the requester.
ISO copyright office
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Published in Switzerland
ii © ISO 2019 – All rights reserved

ISO 21479:2019(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms, definitions and abbreviated terms . 1
3.1 Terms and definitions . 1
3.2 Abbreviated terms . 2
4 Principle . 2
5 Apparatus and reagents . 2
5.1 Apparatus . 2
5.2 Reagents. 3
6 Sampling strategies . 3
7 Sampling of leaf tissues . 3
8 Obtaining, extraction and analyses of FAMES . 4
8.1 Contamination control . 4
8.2 Obtaining and extraction of FAMES from plant leaves . 4
8.3 Analysis of FAMES . 4
9 Test report . 6
9.1 A reference to this document, i.e. ISO 21479 . 6
9.2 Description of the site and areas analysed . 6
9.3 Leaf sampling . 6
9.4 Fatty acid composition . 6
9.5 Conclusion . 6
Annex A (informative) Results of the ring test . 7
Annex B (informative) Assessment of soil quality by determining the Omega-3 index of
Lactuca sativa seedlings grown ex situ under controlled conditions .14
Annex C (informative) Plant species previously successfully used to assess soils of
contaminated sites (organic and/or metals) .16
Annex D (informative) Variation of the Omega-3 index as function of harvest time, plant
size and leaf development .17
Annex E (informative) Effect of the quantity of foliar tissues on the FAMES composition .19
Annex F (informative) Example of chromatogram obtained after the FAMES analysis of
foliar tissues .20
Annex G (informative) Recommended mathematical method to rate soils of areas when
some sampled plant species are not found in all areas .21
Bibliography .23
ISO 21479:2019(E)
Foreword
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bodies (ISO member bodies). The work of preparing International Standards is normally carried out
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ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
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Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
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