EN 26461-1:1993

Water quality - Detection and enumeration of the spores of sulfite-reducing anaerobes (clostridia) - Part 1: Method by enrichment in a liquid medium (ISO 6461-1:1986)

The procedure is applicable to all types of water, including turbid water. The principle covers several steps from selection by applying heat to destroy vegetative bacteria to the indication by inoculating volumes of the sample into media followed by incubation at 27 ± °C in anaerobic conditions.

Wasserbeschaffenheit - Nachweis und Zählung der Sporen sulfitreduzierender Anaerobier (Clostridien) - Teil 1: Flüssigkeitsanreicherung (ISO 6461-1:1986)

Qualité de l'eau - Recherche et dénombrement des spores de micro-organismes anaérobies sulfito-réducteurs (clostridia) - Partie 1: Méthode par enrichissement dans un milieu liquide (ISO 6461-1:1986)

La présente partie de l'ISO 6461 spécifie une méthode pour la recherche et le dénombrement des spores de micro-organismes anaérobies sulfito-réducteurs (clostridia) par enrichissement dans un milieu liquide.
Le principe de la méthode est applicable à tous les types d'eau, y compris les eaux troubles.

Kakovost vode - Ugotavljanje in štetje spor sulfit reducirajočih anaerobov (klostridiji) - 1. del: Metoda z obogatitvijo v tekočem gojišču (ISO 6461-1:1986)

General Information

• Status: Published
• Publication Date: 24-Jan-1993
• ICS: 13.060.30 - Sewage water
• Technical Committee: CEN/TC 230 - Water analysis
• Drafting Committee: CEN/TC 230 - Water analysis
• Current Stage: 9093 - Decision to confirm - Review Enquiry
• Start Date: 21-May-2008
• Completion Date: 21-May-2008
• Ref Project: SIST EN 26461-1:1997 - Water quality - Detection and enumeration of the spores of sulfite-reducing anaerobes (clostridia) - Part 1: Method by enrichment in a liquid medium (ISO 6461-1:1986)

Standards Content (Sample)

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.DQDHURERYWasserbeschaffenheit - Nachweis und Zählung der Sporen sulfitreduzierender Anaerobier (Clostridien) - Teil 1: Flüssigkeitsanreicherung (ISO 6461-1:1986)Qualité de l'eau - Recherche et dénombrement des spores de micro-organismes anaérobies sulfito-réducteurs (clostridia) - Partie 1: Méthode par enrichissement dans un milieu liquide (ISO 6461-1:1986)Water quality - Detection and enumeration of the spores of sulfite-reducing anaerobes (clostridia) - Part 1: Method by enrichment in a liquid medium (ISO 6461-1:1986)13.060.70Preiskava bioloških lastnosti vodeExamination of biological properties of waterICS:Ta slovenski standard je istoveten z:EN 26461-1:1993SIST EN 26461-1:1997en01-oktober-1997SIST EN 26461-1:1997SLOVENSKI
STANDARD



SIST EN 26461-1:1997



SIST EN 26461-1:1997



SIST EN 26461-1:1997



International Standard 646111 INTERNATIONAL ORGANIZATION FOR STANDARDIZATION.MEHt~YHAPO~HAR OPrAHkl3AL&lR I-IO CTAHAAPTM3AUMM~ORGANlSATION INTERNATIONALE DE NORMALISATION Water quality - Qetection and enumeration of the spores of sulfite-reducing anaerobes (clostridia) - Part 1 : Method by enrichment in a liquid medium Qual&! de l’eau - Recherche et denombrement des spores de micro-organismes anakrobies sulfito-r6ducteurs (clostridia) - Partie I : M&hode par enrichkement dans un milieu liquide First edition - 1986-02-01 w - ca 8 UDC 543.39 : 579.852.13 Ref. No. ISO 6461/1-1986 (E) Descriptors : water, quality, tests, microbiological analysis, determination, micro-organisms, sulphite reducing bacteria. Price based on 3 pages SIST EN 26461-1:1997



Foreword ISO (the International Organization for Standardization) is a worldwide federation of national Standards bodies (ISO member bedies). The work of preparing International Standards is normally carried out through ISO technical committees. Esch member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, govern- mental and non-governmental, in liaison with ISO, also take part in the work. Draft International Standards adopted by the technical committees are circulated to the member bodies for approval before their acceptance as International Standards by the ISO Council. They are approved in accordance with ISO procedures requiring at least 75 % approval by the member bodies voting. International Standard ISO 6461/1 was prepared by Technical Committee ISO/TC 147, Water guality. Users should note that all International Standards undergo revision from time to time and that any reference made herein to any other International Standard implies its latest edition, unless othetwise stated. International Organkation for Standardkation, 1986 Printed in Switzerland SIST EN 26461-1:1997



INTERNATIONAL STANDARD ISO 6461/1-1986 (E) Water quality - Detection and enumeration of the spores of sulfite-reducing anaerobes (clostridia) - Part 1 : Method by enrichment in a liquid medium 0 Introduction The spores of sulfite-reducing anaerobes (clostridia) are wide- spread in the environment. They are present in human and animal faecal matter, in waste water and in soil. Unlike Escherichia co/i and other coliform organisms, the spores sur- vive in water for long periods as they are more resistant than vegetative forms to the action of Chemical and physical factors. They may thus give an indication of remote or intermittent pollution. They may even be resistant to chlorination at levels which are normally used for the treatment of water, and they are thus useful for control purposes. ISO 6461 consists of the following Parts: Part 1 : Method by enrichment in a liquid medium. Part 2: Method by membrane filtration. 1 Scope This part of ISO 6461 specifies a method for the detection and enumeration of the spores of sulfite-reducing anaerobes (clostridia) by enrichment in a liquid medium. 2 Field of application The method is applicable to all types of water, including turbid water. 3 References ISO 3696, Water for laboratory use - Specifications. ISO 5667, Water qua!‘@ - Sampling - Part 2: Guidance on sampling techniques. Part 3: Guidance on the preservation and handling of samples. ISO 8199, Water qualiiy - General guidance for microbiological examina tion b y enumeration of micro- organisms on culture media. ’ ) 4 Definition For the purpose of this part of ISO 6461, the following defi- nition applies. ciostridia : Sulfite-reducing, Spore-forming, anaerobic micro- organisms which belong to the Bacillaceae family and the genus Clostridium. 5 Principle The detection of spores of sulfite-reducing anaerobes (clostridia) in a specified volume of a water Sample requires the following Steps. 5.1 Selection of spores Selection of spores in the Sample by applying heat for a period of time sufficient to destroy vegetative bacteria. 5.2 Enrichment culture Detection and enumeration
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