EN 16924:2017
(Main)Foodstuffs - Determination of zearalenone in edible vegetable oils by LC-FLD or LC-MS/MS
Foodstuffs - Determination of zearalenone in edible vegetable oils by LC-FLD or LC-MS/MS
This European Standard describes a procedure for the determination of the zearalenone content in edible vegetable oils specifically maize germ oil by either of the following techniques: High performance liquid chromatography with fluorescence detection (LC-FLD) or high performance liquid chromatography with tandem mass spectrometry (LC-MS/MS) after basic extraction of the diluted oil.
The method has been validated for zearalenone in naturally contaminated maize germ oil at levels of 61,2 µg/kg to 515 µg/kg [5].
Laboratory experiences [6] have shown that this method is also applicable to other vegetable oils such as wheat germ oil (n = 4), sunflower oil (n = 5), pumpkin seed oil (n = 1), soybean oil (n = 5), hemp seed oil (n = 5), rape seed oil (n = 11), and mixed oils including maize germ oil (n = 3). However occasionally, samples can result in interferences in the FLD-chromatograms. In this case, the detection with MS/MS is recommended.
Lebensmittel - Bestimmung von Zearalenon in pflanzlichen Speiseölen mit LC-FLD oder LC-MS/MS
Diese Europäische Norm beschreibt ein Verfahren für die Bestimmung des Zearalenongehalts in pflanzlichen Speiseölen, insbesondere in Maiskeimöl, mit einem der beiden folgenden Verfahren: Hochleistungsflüssig¬chromatographie mit Fluoreszenzdetektion (LC FLD) oder Hochleistungsflüssigchromatographie mit Tandem-Massenspektrometrie (LC MS/MS) nach basischer Extraktion des verdünnten Öles.
Das Verfahren wurde für Zearalenon in natürlich kontaminiertem Maiskeimöl bei Konzentrationen von 61,2 µg/kg bis 515 µg/kg validiert [5].
Laboruntersuchungen [6] haben gezeigt, dass das Verfahren auch für andere pflanzliche Öle, wie z. B. Weizenkeimöl (n = 4), Sonnenblumenöl (n = 5), Kürbiskernöl (n = 1), Sojaöl (n = 5), Hanfsamenöl (n = 5), Rapsöl (n = 11) sowie Pflanzenölmischungen (n = 3), die Maiskeimöl enthalten, anwendbar ist. Jedoch können Proben gelegentlich zu Interferenzen in den FLD Chromatogrammen führen. In diesem Fall wird die Detektion mit MS/MS empfohlen.
Produits alimentaires - Dosage de la zéaralénone dans les huiles végétales alimentaires par CL-DF ou CL-SM/SM
La présente Norme européenne décrit un mode opératoire de détermination de la teneur en zéaralénone dans les huiles végétales alimentaires, notamment l’huile de germes de maïs, par l’une des techniques suivantes : chromatographie liquide à haute performance avec détection de fluorescence (CL-DF) ou chromatographie liquide à haute performance couplée à une spectrométrie de masse en tandem (CL-SM/SM) après extraction basique de l’huile diluée.
La méthode a été validée pour la zéaralénone présente dans l’huile de germes de maïs naturellement contaminée à des niveaux de 61,2 µg/kg à 515 µg/kg [5].
Les expériences menées en laboratoire [6] sans doute ont démontré que cette méthode est également applicable à d’autres huiles végétales telles que l’huile de germes de blé (n = 4), l’huile de tournesol (n = 5), l’huile de pépins de courge (n = 1), l’huile de soja (n = 5), l’huile de chanvre (n = 5), l’huile de colza (n = 11) et les mélanges d’huiles tels que l’huile de germes de maïs (n = 3). Toutefois, les échantillons peuvent parfois provoquer des interférences dans les chromatogrammes issus de la détection par fluorescence (DF). Dans ce cas, il est recommandé d’utiliser la détection par SM/SM.
Živila - Določevanje zearalenona v jedilnih rastlinskih oljih z LC-FLD ali LC-MS/MS
Ta evropski standard opisuje postopek za določanje vsebnosti zearalenona v jedilnih rastlinskih oljih, zlasti v olju iz koruznih kalčkov, z eno od naslednjih tehnik: tekočinska kromatografija visoke ločljivosti s fluorescenčno detekcijo (LC-FLD) ali tekočinska kromatografija visoke ločljivosti s tandemsko masno spektrometrijo (LC-MS/MS) po osnovni ekstrakciji razredčenega olja.
Ta metoda je bila potrjena za zearalenon v naravno kontaminiranem olju iz koruznih kalčkov pri koncentracijah 61,2–515 μg/kg [5].
Laboratorijske izkušnje [6] kažejo, da se ta metoda uporablja tudi za rastlinska olja, kot so olje pšeničnih kalčkov (n = 4), sončnično olje (n = 5), bučno olje (n = 1), sojino olje (n = 5), konopljino olje (n = 5), olje oljne ogrščice (n = 11) in mešana olja, vključno z olji iz koruznih kalčkov (n = 3). Vendar pa vzorci lahko občasno povzročijo motnje v kromatogramih FLD. V tem primeru je priporočena detekcija z MS/MS.
General Information
Standards Content (Sample)
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Lebensmittel - Bestimmung von Zearalenon in pflanzlichen Speiseölen mit LC-FLD oder LC-MS/MSDenrées alimentaires - Dosage de la zéaralénone dans les huiles végétales alimentaires par CL-FLD ou CL-SM/SMFoodstuffs - Determination of zearalenone in edible vegetable oils by LC-FLD or LC-MS/MS67.200.10Animal and vegetable fats and oilsICS:Ta slovenski standard je istoveten z:EN 16924:2017SIST EN 16924:2017en,fr,de01-september-2017SIST EN 16924:2017SLOVENSKI
STANDARD
SIST EN 16924:2017
EUROPEAN STANDARD NORME EUROPÉENNE EUROPÄISCHE NORM
EN 16924
May
t r s y ICS
x yä t r rä s r English Version
Foodstuffs æ Determination of zearalenone in edible Produits alimentaires æ Dosage de la zéaralénone dans les huiles végétales alimentaires par CLæFLD ou CLæ Lebensmittel æ Bestimmung von Zearalenon in This European Standard was approved by CEN on
t y February
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egulations which stipulate the conditions for giving this European Standard the status of a national standard without any alterationä Upætoædate lists and bibliographical references concerning such national standards may be obtained on application to the CENæCENELEC Management Centre or to any CEN memberä
translation under the responsibility of a CEN member into its own language and notified to the CENæCENELEC Management Centre has the same status as the official versionsä
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t r s y CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Membersä Refä Noä EN
s x { t vã t r s y ESIST EN 16924:2017
EN 16924:2017 (E) 2 Contents Page European foreword . 3 Introduction . 4 1 Scope . 5 2 Normative references . 5 3 Principle . 5 4 Reagents . 5 5 Apparatus and equipment . 8 6 Procedure. 10 7 Calculation . 12 8 Precision . 14 9 Test report . 15 Annex A (informative)
Typical chromatograms . 16 Annex B (informative)
Example conditions for suitable LC-MS/MS systems . 22 Annex C (informative)
Precision data . 27 Bibliography . 30
SIST EN 16924:2017
EN 16924:2017 (E) 3 European foreword This document (EN 16924:2017) has been prepared by Technical Committee CEN/TC 275 “Food analysis - Horizontal methods”, the secretariat of which is held by DIN. This European Standard shall be given the status of a national standard, either by publication of an identical
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