EN ISO 7937:2004
(Main)Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of Clostridium perfringens - Colony-count technique (ISO 7937:2004)
Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of Clostridium perfringens - Colony-count technique (ISO 7937:2004)
ISO 7937:2004 describes a horizontal method for the enumeration of viable Clostridium perfringens. It is applicable to products intended for human consumption and the feeding of animals, and environmental samples in the area of food production and food handling.
Mikrobiologie von Lebensmitteln und Futtermitteln - Horizontales Verfahren zur Zählung von Clostridium perfringens - Koloniezählverfahren (ISO 7937:2004)
Anwendungsbereich
Diese Internationale Norm legt ein horizontales Verfahren zur Zählung von lebensfähigen Clostridium perfringens fest. Diese Internationale Norm gilt
- für Erzeugnisse, die für den menschlichen Verzehr oder als Futtermittel bestimmt sind, und
für Umgebungsproben im Bereich der Herstellung und der Behandlung von Lebensmitteln.
Microbiologie des aliments - Méthode horizontale pour le dénombrement de Clostridium perfringens - Technique par comptage des colonies (ISO 7937:2004)
L'ISO 7937:2004 décrit une méthode horizontale pour le dénombrement des Clostridium perfringens revivifiables. La présente norme est applicable aux produits pour l'alimentation humaine et animale, et aux échantillons d'environnement pour la production et la distribution des aliments.
Mikrobiologija živil in krme - Horizontalna metoda za ugotavljanje števila Clostridium perfringens - Tehnika štetja kolonij (ISO 7937:2004)
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
01-januar-2005
1DGRPHãþD
SIST EN 13401:1999
Mikrobiologija živil in krme - Horizontalna metoda za ugotavljanje števila
Clostridium perfringens - Tehnika štetja kolonij (ISO 7937:2004)
Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of
Clostridium perfringens - Colony-count technique (ISO 7937:2004)
Mikrobiologie von Lebensmitteln und Futtermitteln - Horizontales Verfahren zur Zählung
von Clostridium perfringens - Koloniezählverfahren (ISO 7937:2004)
Microbiologie des aliments - Méthode horizontale pour le dénombrement de Clostridium
perfringens - Technique par comptage des colonies (ISO 7937:2004)
Ta slovenski standard je istoveten z: EN ISO 7937:2004
ICS:
07.100.30 Mikrobiologija živil Food microbiology
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EUROPEAN STANDARD
EN ISO 7937
NORME EUROPÉENNE
EUROPÄISCHE NORM
August 2004
ICS 07.100.30 Supersedes EN 13401:1999
English version
Microbiology of food and animal feeding stuffs - Horizontal
method for the enumeration of Clostridium perfringens - Colony-
count technique (ISO 7937:2004)
Microbiologie des aliments - Méthode horizontale pour le Mikrobiologie von Lebensmitteln und Futtermitteln -
dénombrement de Clostridium perfringens - Technique par Horizontales Verfahren zur Zählung von Clostridium
comptage des colonies (ISO 7937:2004) perfringens - Koloniezählverfahren (ISO 7937:2004)
This European Standard was approved by CEN on 14 August 2004.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the Central Secretariat or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the Central Secretariat has the same status as the official
versions.
CEN members are the national standards bodies of Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France,
Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Slovakia,
Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36 B-1050 Brussels
© 2004 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 7937:2004: E
worldwide for CEN national Members.
Foreword
This document (EN ISO 7937:2004) has been prepared by Technical Committee ISO/TC 34
"Agricultural food products" in collaboration with Technical Committee CEN/TC 275 "Food
analysis - Horizontal methods", the secretariat of which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of
an identical text or by endorsement, at the latest by February 2005, and conflicting national
standards shall be withdrawn at the latest by February 2005.
This document supersedes EN 13401:1999.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of
the following countries are bound to implement this European Standard: Austria, Belgium,
Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary,
Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland,
Portugal, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
Endorsement notice
The text of ISO 7937:2004 has been approved by CEN as EN ISO 7937:2004 without any
modifications.
INTERNATIONAL ISO
STANDARD 7937
Third edition
2004-08-15
Microbiology of food and animal feeding
stuffs — Horizontal method for the
enumeration of Clostridium
perfringens — Colony-count technique
Microbiologie des aliments — Méthode horizontale pour le
dénombrement de Clostridium perfringens — Technique par comptage
des colonies
Reference number
ISO 7937:2004(E)
©
ISO 2004
ISO 7937:2004(E)
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ii © ISO 2004 – All rights reserved
ISO 7937:2004(E)
Contents Page
Foreword. iv
Introduction . v
1 Scope. 1
2 Normative references. 1
3 Terms and definitions. 2
4 Principle. 2
5 Diluent, culture media and reagents . 2
6 Apparatus and glassware. 7
7 Sampling. 7
8 Preparation of test sample. 7
9 Procedure. 7
10 Expression of results. 9
11 Test report. 11
Annex A (informative) Results of interlaboratory test . 12
Bibliography . 16
ISO 7937:2004(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies
(ISO member bodies). The work of preparing International Standards is normally carried out through ISO
technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the
International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International Standards
adopted by the technical committees are circulated to the member bodies for voting. Publication as an
International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights.
ISO 7937 was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 9,
Microbiology.
This third edition cancels and replaces the second edition (ISO 7937:1997, EN 13401:1999), which has been
technically revised.
iv © ISO 2004 – All rights reserved
ISO 7937:2004(E)
Introduction
Because of the large variety of food and feed products, this horizontal method may not be appropriate in every
detail for certain products. In this case, different methods that are specific to these products may be used if
absolutely necessary for justified technical reasons. Nevertheless, every attempt should be made to apply this
horizontal method as far as possible.
When this International Standard is next reviewed, account will be taken of all information then available
regarding the extent to which this horizontal method has been followed and the reasons for deviations from
this method in the case of particular products.
The harmonization of test methods cannot be immediate and, for certain groups of products, International
Standards and/or national standards may already exist that do not comply with this horizontal method. It is
hoped that when such standards are reviewed they will be changed to comply with this international Standard
so that eventually the only remaining departures from this horizontal method will be those necessary for
well-established technical reasons.
INTERNATIONAL STANDARD ISO 7937:2004(E)
Microbiology of food and animal feeding stuffs — Horizontal
method for the enumeration of Clostridium perfringens —
Colony-count technique
1 Scope
This International Standard describes a horizontal method for the enumeration of viable Clostridium
perfringens. It is applicable to
products intended for human consumption and the feeding of animals, and
environmental samples in the area of food production and food handling.
2 Normative references
The following referenced documents are indispensable for the application of this document. For dated
references, only the edition cited applies. For undated references, the latest edition of the referenced
document (including any amendments) applies.
ISO 6887-1, Microbiology of food and animal feeding stuffs — Preparation of test samples, initial suspension
and decimal dilutions for microbiological examination — Part 1: General rules for the preparation of the initial
suspension and decimal dilutions
ISO 6887-2, Microbiology of food and animal feeding stuffs — Preparation of test samples, initial suspension
and decimal dilutions for microbiological examination — Part 2: Specific rules for the preparation of meat and
meat products
ISO 6887-3, Microbiology of food and animal feeding stuffs — Preparation of test samples, initial suspension
and decimal dilutions for microbiological examination — Part 3: Specific rules for the preparation of fish and
fishery products
ISO 6887-4, Microbiology of food and animal feeding stuffs — Preparation of test samples, initial suspension
and decimal dilutions for microbiological examination — Part 4: Specific rules for the preparation of products
other than milk and milk products meat and meat products, and fish and fishery products
ISO 7218, Microbiology of food and animal feeding stuffs — General rules for microbiological examinations
ISO 8261, Milk and milk products — General guidance for the preparation of test samples, initial suspensions
and decimal dilutions for microbiological examination
ISO/TS 11133-1, Microbiology of food and animal feeding stuffs — Guidelines on preparation and production
of culture media — Part 1 : General guidelines on quality assurance for the preparation of culture media in the
laboratory
ISO/TS 11133-2:2003, Microbiology of food and animal feeding stuffs — Guidelines on preparation and
production of culture media — Part 2: Practical guidelines on performance testing of culture media
ISO 7937:2004(E)
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1
Clostridium perfringens
C. perfringens
bacteria that form characteristic colonies (black precipitate, caused by the reduction of sulfite to sulfide, which
colours the colonies black) in the specified selective medium, and which give positive confirmatory reactions
when the test is carried out by either of two techniques specified in this International Standard
3.2
enumeration of C. p
...
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