EN ISO 18363-1:2021

Animal and vegetable fats and oils - Determination of fatty-acid-bound chloropropanediols (MCPDs) and glycidol by GC/MS - Part 1: Method using fast alkaline transesterification and measurement for 3-MCPD and differential measurement for glycidol (ISO 18363-1:2015)

ISO 18363-1:2015 describes a procedure for the indirect determination of 3-MCPD esters (bound 3-MCPD) and possible free 3-MCPD after alkaline catalysed ester cleavage and derivatization with phenylboronic acid (PBA). Furthermore, this part of ISO 18363 enables the indirect determination of glycidyl esters (bound glycidol) under the assumption that no other substances are present that react at room temperature with inorganic chloride to generate 3-MCPD.
ISO 18363-1:2015 is applicable to solid and liquid fats and oils. Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this part of ISO 18363.

Tierische und pflanzliche Fette und Öle - Bestimmung von fettsäuregebundenen Chlorpropandiol (MCPD) und Glycidol mittels GC/MS - Teil 1: Verfahren mittels schneller alkalischer Umesterung und Messung für 3 MCPD und Differenzmessung für Glycidol (ISO 18363-1:2015)

Dieser Teil von ISO 18363 beschreibt ein Verfahren für die indirekte Bestimmung von 3 MCPD-Estern (gebundenes 3 MCPD) und möglichem freiem 3 MCPD nach alkalisch katalysierter Esterspaltung und Derivatisierung mit Phenylboronsäure (en: phenylboronic acid, PBA). Außerdem ermöglicht dieser Teil von ISO 18363 die indirekte Bestimmung von Glycidylestern (gebundenes Glycidol) unter der Annahme, dass keine anderen Substanzen vorhanden sind, die bei Raumtemperatur mit anorganischem Chlorid zu 3 MCPD reagieren.
Dieser Teil von ISO 18363 ist auf feste und flüssige Fette und Öle anwendbar. Milch und Milcherzeugnisse (oder Fett aus Milch und Milcherzeugnissen) sind vom Anwendungsbereich dieses Teils von ISO 18363 ausgenommen.

Corps gras d'origines animale et végétale - Détermination des esters de chloropropanediols (MCPD) et d'acides gras et des esters de glycidol et d'acides gras par CPG/SM - Partie 1: Méthode par transestérification alcaline rapide et mesure pour le 3-MCPD et par mesure différentielle pour le glycidol (ISO 18363-1:2015)

L'ISO 18363-1:2015 décrit un mode opératoire permettant la détermination indirecte des esters de 3-MCPD (ou 3-MCPD lié) et des éventuels 3-MCPD libres après clivage des esters par catalyse alcaline et dérivatisation à l'acide phénylboronique (PBA). L'ISO 18363-1:2015 définit également la détermination indirecte des esters de glycidol (ou glycidol lié) en partant de l'hypothèse qu'aucune autre substance présente ne réagit, à température ambiante, avec le chlorure inorganique pour générer du 3-MCPD.
L'ISO 18363-1:2015 est applicable aux corps gras solides et liquides. Le lait et les produits laitiers (ou les corps gras issus du lait et des produits laitiers) sont exclus du domaine d'application de l'ISO 18363-1:2015.

Živalske in rastlinske maščobe ter olja - Določevanje maščobnokislinsko vezanih kloropropandiolov (MCPD) in glicidola z GC/MS - 1. del: Metoda z uporabo hitre alkalne transesterifikacije in meritve 3-MCPD ter diferencialne meritve glicidola (ISO 18363-1:2015)

Ta analitični postopek določa tekočinsko kromatografijo visoke ločljivosti z ultravijolično (UV) detekcijo (RP-HPLC-UV) z reverzno fazo za hkratno določevanje štirih dovoljenih karotenoidov v krmni mešanici za ribe, in sicer astaksantina (AXN), kantaksantina (CXN), adonirubina (ADR) in astaksantin dimetildisukcinata (AXN DMDS) ter šestih odobrenih karotenoidov v krmi za perutnino, in sicer kantaksantina (CXN); kapsantina (CSN), etil ester beta-apo-8'-karotenojske kisline (BACARE), citranaksantina (CIXN), luteina (LUT) in zeaksantina (ZEA), v ravneh od pribl. 2 do pribl. 4500 mg/kg (odvisno od karotenoida). V področje uporabe je dodan tudi beta-karoten (BCAR), dovoljen v krmnih mešanicah za vse živalske vrste. Analitski postopek je primeren za določanje količine navedenih karotenoidov in potrditev označevanja. Postopek se uporablja za naravne in sintetične krmne dodatke.
Ksantofilni estri, kot so lutein, zeaksantin in kapsantin, ki bi lahko bili prisotni v posamičnih krmilih, niso dovoljeni krmni dodatki in zato ne spadajo na področje uporabe te metode.

General Information

• Status: Published
• Publication Date: 14-Dec-2021
• Withdrawal Date: 29-Jun-2022
• ICS: 67.200.10 - Animal and vegetable fats and oils
• Technical Committee: CEN/TC 307 - Oilseeds, vegetables and animal fats and oils and their by-products - Methods of sampling and analys
• Drafting Committee: CEN/TC 307 - Oilseeds, vegetables and animal fats and oils and their by-products - Methods of sampling and analys
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 15-Dec-2021
• Due Date: 27-Oct-2023
• Completion Date: 15-Dec-2021
• Ref Project: SIST EN ISO 18363-1:2022 - Animal and vegetable fats and oils - Determination of fatty-acid-bound chloropropanediols (MCPDs) and glycidol by GC/MS - Part 1: Method using fast alkaline transesterification and measurement for 3-MCPD and differential measurement for glycidol (ISO 18363-1:2015)

Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN ISO 18363-1:2022
01-februar-2022
Živalske in rastlinske maščobe ter olja - Določevanje maščobnokislinsko vezanih
kloropropandiolov (MCPD) in glicidola z GC/MS - 1. del: Metoda z uporabo hitre
alkalne transesterifikacije in meritve 3-MCPD ter diferencialne meritve glicidola
(ISO 18363-1:2015)
Animal and vegetable fats and oils - Determination of fatty-acid-bound
chloropropanediols (MCPDs) and glycidol by GC/MS - Part 1: Method using fast alkaline
transesterification and measurement for 3-MCPD and differential measurement for
glycidol (ISO 18363-1:2015)
Tierische und pflanzliche Fette und Öle - Bestimmung von fettsäuregebundenen
Chlorpropandiol (MCPD) und Glycidol mittels GC/MS - Teil 1: Verfahren mittels schneller
alkalischer Umesterung und Messung für 3 MCPD und Differenzmessung für Glycidol
(ISO 18363 1:2015)
Corps gras d'origines animale et végétale - Détermination des esters de
chloropropanediols (MCPD) et d'acides gras et des esters de glycidol et d'acides gras
par CPG/SM - Partie 1: Méthode par transestérification alcaline rapide et mesure pour le
3-MCPD et par mesure différentielle pour le glycidol (ISO 18363-1:2015)
Ta slovenski standard je istoveten z: EN ISO 18363-1:2021
ICS:
67.200.10 Rastlinske in živalske Animal and vegetable fats
maščobe in olja and oils
SIST EN ISO 18363-1:2022 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN ISO 18363-1:2022

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SIST EN ISO 18363-1:2022


EN ISO 18363-1
EUROPEAN STANDARD

NORME EUROPÉENNE

December 2021
EUROPÄISCHE NORM
ICS 67.200.10
English Version

Animal and vegetable fats and oils - Determination of fatty-
acid-bound chloropropanediols (MCPDs) and glycidol by
GC/MS - Part 1: Method using fast alkaline
transesterification and measurement for 3-MCPD and
differential measurement for glycidol (ISO 18363-1:2015)
Corps gras d'origines animale et végétale - Tierische und pflanzliche Fette und Öle - Bestimmung
Détermination des esters de chloropropanediols von fettsäuregebundenen Chlorpropandiol (MCPD)
(MCPD) et d'acides gras et des esters de glycidol et und Glycidol mittels GC/MS - Teil 1: Verfahren mittels
d'acides gras par CPG/SM - Partie 1: Méthode par schneller alkalischer Umesterung und Messung für 3
transestérification alcaline rapide et mesure pour le 3- MCPD und Differenzmessung für Glycidol (ISO 18363
MCPD et par mesure différentielle pour le glycidol (ISO 1:2015)
18363-1:2015)
This European Standard was approved by CEN on 5 December 2021.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
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Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.





EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2021 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 18363-1:2021 E
worldwide for CEN national Members.

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SIST EN ISO 18363-1:2022
EN ISO 18363-1:2021 (E)
Contents Page
European foreword . 3

2

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SIST EN ISO 18363-1:2022
EN ISO 18363-1:2021 (E)
European foreword
The text of ISO 18363-1:2015 has been prepared by Technical Committee ISO/TC 34 "Food products” of
the International Organization for Standardization (ISO) and has been taken over as EN ISO 18363-
1:2021 by Technical Committee CEN/TC 307 “Oilseeds, vegetable and animal fats and oils and their by-
products - Methods of sampling and analysis” the secretariat of which is held by AFNOR.
This European Standard shall be given the status of a national standar
...