EN ISO 18363-1:2021

Animal and vegetable fats and oils - Determination of fatty-acid-bound chloropropanediols (MCPDs) and glycidol by GC/MS - Part 1: Method using fast alkaline transesterification and measurement for 3-MCPD and differential measurement for glycidol (ISO 18363-1:2015)

ISO 18363-1:2015 describes a procedure for the indirect determination of 3-MCPD esters (bound 3-MCPD) and possible free 3-MCPD after alkaline catalysed ester cleavage and derivatization with phenylboronic acid (PBA). Furthermore, this part of ISO 18363 enables the indirect determination of glycidyl esters (bound glycidol) under the assumption that no other substances are present that react at room temperature with inorganic chloride to generate 3-MCPD.
ISO 18363-1:2015 is applicable to solid and liquid fats and oils. Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this part of ISO 18363.

Tierische und pflanzliche Fette und Öle - Bestimmung von fettsäuregebundenen Chlorpropandiol (MCPD) und Glycidol mittels GC/MS - Teil 1: Verfahren mittels schneller alkalischer Umesterung und Messung für 3 MCPD und Differenzmessung für Glycidol (ISO 18363-1:2015)

Dieser Teil von ISO 18363 beschreibt ein Verfahren für die indirekte Bestimmung von 3 MCPD-Estern (gebundenes 3 MCPD) und möglichem freiem 3 MCPD nach alkalisch katalysierter Esterspaltung und Derivatisierung mit Phenylboronsäure (en: phenylboronic acid, PBA). Außerdem ermöglicht dieser Teil von ISO 18363 die indirekte Bestimmung von Glycidylestern (gebundenes Glycidol) unter der Annahme, dass keine anderen Substanzen vorhanden sind, die bei Raumtemperatur mit anorganischem Chlorid zu 3 MCPD reagieren.
Dieser Teil von ISO 18363 ist auf feste und flüssige Fette und Öle anwendbar. Milch und Milcherzeugnisse (oder Fett aus Milch und Milcherzeugnissen) sind vom Anwendungsbereich dieses Teils von ISO 18363 ausgenommen.

Corps gras d'origines animale et végétale - Détermination des esters de chloropropanediols (MCPD) et d'acides gras et des esters de glycidol et d'acides gras par CPG/SM - Partie 1: Méthode par transestérification alcaline rapide et mesure pour le 3-MCPD et par mesure différentielle pour le glycidol (ISO 18363-1:2015)

L'ISO 18363-1:2015 décrit un mode opératoire permettant la détermination indirecte des esters de 3-MCPD (ou 3-MCPD lié) et des éventuels 3-MCPD libres après clivage des esters par catalyse alcaline et dérivatisation à l'acide phénylboronique (PBA). L'ISO 18363-1:2015 définit également la détermination indirecte des esters de glycidol (ou glycidol lié) en partant de l'hypothèse qu'aucune autre substance présente ne réagit, à température ambiante, avec le chlorure inorganique pour générer du 3-MCPD.
L'ISO 18363-1:2015 est applicable aux corps gras solides et liquides. Le lait et les produits laitiers (ou les corps gras issus du lait et des produits laitiers) sont exclus du domaine d'application de l'ISO 18363-1:2015.

Živalske in rastlinske maščobe ter olja - Določevanje maščobnokislinsko vezanih kloropropandiolov (MCPD) in glicidola z GC/MS - 1. del: Metoda z uporabo hitre alkalne transesterifikacije in meritve 3-MCPD ter diferencialne meritve glicidola (ISO 18363-1:2015)

Ta analitični postopek določa tekočinsko kromatografijo visoke ločljivosti z ultravijolično (UV) detekcijo (RP-HPLC-UV) z reverzno fazo za hkratno določevanje štirih dovoljenih karotenoidov v krmni mešanici za ribe, in sicer astaksantina (AXN), kantaksantina (CXN), adonirubina (ADR) in astaksantin dimetildisukcinata (AXN DMDS) ter šestih odobrenih karotenoidov v krmi za perutnino, in sicer kantaksantina (CXN); kapsantina (CSN), etil ester beta-apo-8'-karotenojske kisline (BACARE), citranaksantina (CIXN), luteina (LUT) in zeaksantina (ZEA), v ravneh od pribl. 2 do pribl. 4500 mg/kg (odvisno od karotenoida). V področje uporabe je dodan tudi beta-karoten (BCAR), dovoljen v krmnih mešanicah za vse živalske vrste. Analitski postopek je primeren za določanje količine navedenih karotenoidov in potrditev označevanja. Postopek se uporablja za naravne in sintetične krmne dodatke.
Ksantofilni estri, kot so lutein, zeaksantin in kapsantin, ki bi lahko bili prisotni v posamičnih krmilih, niso dovoljeni krmni dodatki in zato ne spadajo na področje uporabe te metode.

General Information

• Status: Published
• Publication Date: 14-Dec-2021
• Withdrawal Date: 29-Jun-2022
• ICS: 67.200.10 - Animal and vegetable fats and oils
• Technical Committee: CEN/TC 307 - Oilseeds, vegetables and animal fats and oils and their by-products - Methods of sampling and analys
• Drafting Committee: CEN/TC 307 - Oilseeds, vegetables and animal fats and oils and their by-products - Methods of sampling and analys
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 15-Dec-2021
• Due Date: 27-Oct-2023
• Completion Date: 15-Dec-2021
• Ref Project: SIST EN ISO 18363-1:2022 - Animal and vegetable fats and oils - Determination of fatty-acid-bound chloropropanediols (MCPDs) and glycidol by GC/MS - Part 1: Method using fast alkaline transesterification and measurement for 3-MCPD and differential measurement for glycidol (ISO 18363-1:2015)

Standards Content (Sample)

SLOVENSKI STANDARD
01-februar-2022
Živalske in rastlinske maščobe ter olja - Določevanje maščobnokislinsko vezanih
kloropropandiolov (MCPD) in glicidola z GC/MS - 1. del: Metoda z uporabo hitre
alkalne transesterifikacije in meritve 3-MCPD ter diferencialne meritve glicidola
(ISO 18363-1:2015)
Animal and vegetable fats and oils - Determination of fatty-acid-bound
chloropropanediols (MCPDs) and glycidol by GC/MS - Part 1: Method using fast alkaline
transesterification and measurement for 3-MCPD and differential measurement for
glycidol (ISO 18363-1:2015)
Tierische und pflanzliche Fette und Öle - Bestimmung von fettsäuregebundenen
Chlorpropandiol (MCPD) und Glycidol mittels GC/MS - Teil 1: Verfahren mittels schneller
alkalischer Umesterung und Messung für 3 MCPD und Differenzmessung für Glycidol
(ISO 18363 1:2015)
Corps gras d'origines animale et végétale - Détermination des esters de
chloropropanediols (MCPD) et d'acides gras et des esters de glycidol et d'acides gras
par CPG/SM - Partie 1: Méthode par transestérification alcaline rapide et mesure pour le
3-MCPD et par mesure différentielle pour le glycidol (ISO 18363-1:2015)
Ta slovenski standard je istoveten z: EN ISO 18363-1:2021
ICS:
67.200.10 Rastlinske in živalske Animal and vegetable fats
maščobe in olja and oils
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 18363-1
EUROPEAN STANDARD
NORME EUROPÉENNE
December 2021
EUROPÄISCHE NORM
ICS 67.200.10
English Version
Animal and vegetable fats and oils - Determination of fatty-
acid-bound chloropropanediols (MCPDs) and glycidol by
GC/MS - Part 1: Method using fast alkaline
transesterification and measurement for 3-MCPD and
differential measurement for glycidol (ISO 18363-1:2015)
Corps gras d'origines animale et végétale - Tierische und pflanzliche Fette und Öle - Bestimmung
Détermination des esters de chloropropanediols von fettsäuregebundenen Chlorpropandiol (MCPD)
(MCPD) et d'acides gras et des esters de glycidol et und Glycidol mittels GC/MS - Teil 1: Verfahren mittels
d'acides gras par CPG/SM - Partie 1: Méthode par schneller alkalischer Umesterung und Messung für 3
transestérification alcaline rapide et mesure pour le 3- MCPD und Differenzmessung für Glycidol (ISO 18363
MCPD et par mesure différentielle pour le glycidol (ISO 1:2015)
18363-1:2015)
This European Standard was approved by CEN on 5 December 2021.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2021 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 18363-1:2021 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
The text of ISO 18363-1:2015 has been prepared by Technical Committee ISO/TC 34 "Food products” of
the International Organization for Standardization (ISO) and has been taken over as EN ISO 18363-
1:2021 by Technical Committee CEN/TC 307 “Oilseeds, vegetable and animal fats and oils and their by-
products - Methods of sampling and analysis” the secretariat of which is held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by June 2022, and conflicting national standards shall be
withdrawn at the latest by June 2022.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
Any feedback and questions on this document should be directed to the users’ national standards body.
A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the
United Kingdom.
Endorsement notice
The text of ISO 18363-1:2015 has been approved by CEN as EN ISO 18363-1:2021 without any
modification.
INTERNATIONAL ISO
STANDARD 18363-1
First edition
2015-08-15
Animal and vegetable fats and oils —
Determination of fatty-acid-bound
chloropropanediols (MCPDs) and
glycidol by GC/MS —
Part 1:
Method using fast alkaline
transesterification and measurement
for 3-MCPD and differential
measurement for glycidol
Corps gras d’origines animale et végétale — Détermination des
esters de chloropropanediols (MCPD) et d’acides gras et des esters de
glycidol et d’acides gras —
Partie 1: Méthode par transestérification alcaline rapide et
mesure pour le chloro-3 propane-1,2-diol (3-MCPD) et par mesure
différentielle pour le glycidol
Reference number
ISO 18363-1:2015(E)
©
ISO 2015
ISO 18363-1:2015(E)
© ISO 2015, Published in Switzerland
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form
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ii © ISO 2015 – All rights reserved

ISO 18363-1:2015(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Reagents . 3
5.1 General . 3
5.2 Standard and reference compounds . 3
5.3 Solvents . 3
5.4 Other reagents . 3
6 Apparatus . 4
7 Sample . 4
7.1 Sampling . 4
7.2 Preparation of the test sample . 4
8 Procedure. 5
8.1 Spiking with surrogate standard and homogenization . 5
8.2 Ester cleavage and glycidol transformation . 5
8.3 Matrix clean up . 5
8.4 Derivatization . 5
8.5 Gas chromatography/mass spectrometry references . 6
9 Expression of results . 6
10 Precision . 7
10.1 Interlaboratory test. 7
10.2 Repeatability . 8
10.3 Reproducibility . 8
11 Test report . 8
Annex A (informative) Results of an collaborative trial . 9
Bibliography .11
ISO 18363-1:2015(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the
differe
...