Workplace atmospheres - Volumetric bioaerosol sampling devices - Requirements and test methods

This document specifies requirements and test methods to determine the performance of volumetric sampling devices used to assess bioaerosols in the workplace.
For clean room measurements EN ISO 14698-1 is applicable.

Arbeitsplatzatmosphäre - Volumetrische Probenahmeeinrichtungen für Bioaerosole - Anforderungen und Prüfverfahren

Diese Europäische Norm legt Anforderungen und Prüfverfahren zur Bestimmung der Leistungsfähigkeit von volumetrischen Probenahmeeinrichtungen fest, die zur Bewertung von Bioaerosolen am Arbeitsplatz verwendet werden.
Für Messungen in Reinräumen gilt EN ISO 14698-1.

Air des lieux de travail - Appareils d'échantillonnage volumétrique des bioaérosols - Exigences et méthodes d'essai

La présente Norme européenne spécifie des exigences et des méthodes d'essai permettant de déterminer les performances des appareils d'échantillonnage utilisés pour le contrôle des bioaérosols sur les lieux de travail.
Pour les mesurages en salles propres, le prEN ISO 14698-1 est applicable.

Zrak na delovnem mestu – Naprave za volumetrično vzorčenje bioaerosolov - Zahteve in preskusne metode

General Information

Status
Withdrawn
Publication Date
14-Sep-2004
Current Stage
9960 - Withdrawal effective - Withdrawal
Completion Date
15-Dec-2021

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2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Arbeitsplatzatmosphäre - Volumetrische Probenahmeeinrichtungen für Bioaerosole - Anforderungen und PrüfverfahrenAir des lieux de travail - Appareils d'échantillonnage volumétrique des bioaérosols - Exigences et méthodes d'essaiWorkplace atmospheres - Volumetric bioaerosol sampling devices - Requirements and test methods13.040.30Kakovost zraka na delovnem mestuWorkplace atmospheresICS:Ta slovenski standard je istoveten z:EN 14583:2004SIST EN 14583:2004en,fr,de01-december-2004SIST EN 14583:2004SLOVENSKI

STANDARD
SIST EN 14583:2004

EUROPEAN STANDARDNORME EUROPÉENNEEUROPÄISCHE NORMEN 14583September 2004ICS 13.040.30 English versionWorkplace atmospheres - Volumetric bioaerosol samplingdevices - Requirements and test methodsAir des lieux de travail - Appareils d'échantillonnagevolumétrique des bioaérosols - Exigences et méthodesd'essaiArbeitsplatzatmosphäre - VolumetrischeProbenahmeeinrichtungen für Bioaerosole - Anforderungenund PrüfverfahrenThis European Standard was approved by CEN on 9 July 2004.CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this EuropeanStandard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such nationalstandards may be obtained on application to the Central Secretariat or to any CEN member.This European Standard exists in three official versions (English, French, German). A version in any other language made by translationunder the responsibility of a CEN member into its own language and notified to the Central Secretariat has the same status as the officialversions.CEN members are the national standards bodies of Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France,Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Slovakia,Slovenia, Spain, Sweden, Switzerland and United Kingdom.EUROPEAN COMMITTEE FOR STANDARDIZATIONCOMITÉ EUROPÉEN DE NORMALISATIONEUROPÄISCHES KOMITEE FÜR NORMUNGManagement Centre: rue de Stassart, 36

B-1050 Brussels© 2004 CENAll rights of exploitation in any form and by any means reservedworldwide for CEN national Members.Ref. No. EN 14583:2004: ESIST EN 14583:2004

EN 14583:2004 (E) 2 Contents page Foreword..............................................................................................................................................................3 Introduction.........................................................................................................................................................4 1 Scope......................................................................................................................................................5 2 Normative references............................................................................................................................5 3 Terms and definitions...........................................................................................................................5 4 Abbreviated terms.................................................................................................................................6 5 Requirements.........................................................................................................................................7 6 Test conditions......................................................................................................................................9 7 Environmental conditions for test methods.....................................................................................11 8 Test report............................................................................................................................................11 Annex A (informative)

Bioaerosol sampling................................................................................................12 Annex B (informative)

Example of test facility usable for assessing biological performance of bioaerosol sampling devices..............................................................................................................13 Bibliography......................................................................................................................................................14

SIST EN 14583:2004

EN 14583:2004 (E) 3 Foreword This document (EN 14583:2004) has been prepared by Technical Committee CEN/TC 137 “Assessment of workplace exposure to chemical and biological agents”, the secretariat of which is held by DIN. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by March 2005, and conflicting national standards shall be withdrawn at the latest by March 2005. According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom. SIST EN 14583:2004

EN 14583:2004 (E) 4 Introduction A European Standard is needed to promote the development of new equipment for measurement of micro-organisms in the work environment. This document can also apply to existing equipment. It is intended to specify requirements and methods to determine performance characteristics of sampling devices used to collect bioaerosols from the workplace atmosphere. Examples of test environments and methods will be described and test methods will be provided. WARNING — The use of this European Standard can involve hazardous materials, operations and equipment. This European Standard does not purport to address all of the safety problems associated with its use. It is the responsibility of the user of this European Standard to establish appropriate safety and health practices and to determine the applicability of regulatory limitations prior to use. SIST EN 14583:2004

EN 14583:2004 (E) 5

1 Scope This document specifies requirements and test methods to determine the performance of volumetric sampling devices used to assess bioaerosols in the workplace. For clean room measurements EN ISO 14698-1 is applicable. 2 Normative references The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. EN 1232, Workplace atmospheres — Pumps for personal sampling of chemical agents — Requirements and test methods EN 12919, Workplace atmospheres — Pumps for the sampling of chemical agents with a volume flow rate of over 5 l/min — Requirements and test methods EN 13205, Workplace atmosphere — Assessment of performance of instruments for measurement of airborne particle concentrations EN 50015, Electrical apparatus for potentially explosive atmospheres — Oil immersion ‘o’ EN 50016, Electrical apparatus for potentially explosive atmospheres — Pressurised apparatus ‘p’. EN 50017, Electrical apparatus for potentially explosive atmospheres — Powder filling ‘q’. EN 50020, Electrical apparatus for potentially explosive atmospheres — Intrinsic safety ‘i’. EN 60079-0, Electrical apparatus for potentially explosive atmospheres —Part 0: General requirements (IEC 60079-0: 2004) EN 60079-1, Electrical apparatus for potentially explosive atmospheres — Part 1: Flameproof enclosure ‘d’ (IEC 60079-1:2003) EN 60079-7, Electrical apparatusfor explosive gas atmospheres — Part 7: Increased safety ‘e’(IEC 60079-7: 2001) EN 60079-18, Electrical apparatus for explosive gas atmospheres — Part 18: Construction, test and marking of type of protection encapsulation “m” electrical apparatus (IEC 60079-18:2004) EN 60079-25, Electrical apparatus for explosive gas atmospheres — Part 25: Intrinsically safesystems (IEC 60079-25:2003)

3 Terms and definitions For the purposes of this document, the following terms and definitions apply. 3.1 accuracy closeness of agreement between a test result and the accepted reference value SIST EN 14583:2004

EN 14583:2004 (E) 6 [ISO 3534-1:1993] NOTE The quantity referred to in this document as accuracy provides an estimation of the range around the measured value in which can be found the accepted reference value with the confidence of 95 %. 3.2 bias consistent deviation of the results of a measurement process from the true value of the air quality characteristic itself [EN 482:1994] 3.3 culturable number number of micro-organisms, single cells or aggregates able to form colonies on a solid nutrient medium [EN 13098:2000] 3.4 total number of micro-organisms

number of micro-organisms determined as single organisms (or a corresponding measure) [EN 13098:2000] 3.5 personal sampler device, which samples air in the breathing zone of a person to determine exposure to biological agents NOTE 1 Some sampling devices have integral pumps, and some do not. Where the instrument requires the use of an external pump, the pump is not subject to the requirements of this document. NOTE 2 Adapted from EN 1540. 3.6 particle aerodynamic diameter diameter of a sphere of density 1 g cm-3 with the same terminal velocity due to gravitational force in calm air, as the particle, under the prevailing conditions of temperature, pressure and relative humidity [EN 1540:1998] 3.7 sampling device total equipment used for sampling, e. g. pump, sampling head and sampling substrate 4 Abbreviated terms ATCC American Type Culture Collection BTC bioaerosol test chamber CBS Centraalbureau voor Schimmelcultures CCUG Culture Collection University of Göteborg DSMZ Deutsche Stammsammlung für Mikroorganismen und Zellkulturen HEPA high efficiency particulate aerosol SIST EN 14583:2004

EN 14583:2004 (E) 7 NCTC National Collection of Type Cultures RH relative humidity T temperature 5 Requirements 5.1 General Performance requirements for volumetric sampling pumps shall comply with EN 1232 for low flow rate pumps or EN 12919 for high flow rate pumps. This shall apply both to integral and separate pumps. 5.2 Use in potentially explosive atmospheres When the sampling device covered by this document is to be used in potentially explosive atmospheres, it shall comply with EN 50015 to EN 50017, EN 50020, EN 60079-0, EN 60079-1, EN 60079-7, EN 60079-18 and EN 60079-25. 5.3 Mechanical construction Every sampling device shall be constructed in such a manner that it is easily accessible for regular function checks and that airflow can easily be measured and calibrated. The sampling pump shall maintain the required airflow rate throughout the sampling period. NOTE Material used in the sampling head should be chosen to avoid moisture uptake and electrostatic charges. 5.4 Indicator devices An indicator device shall be provided to show that the sampling device is switched on. If the sampling device has more than one measuring range, the selected range shall be clearly identified. NOTE It is an advantage if elapsed time indicators, low flow rate indicators, flow interrupted indicators are given. 5.5 Adjustments Any equipment (switch, knob, etc.) used for modifying the operating parameters (sampling time, flow rate, etc) of the sampling device shall be protected against involuntary action during sampling. The operational settings should be displayed. 5.6 Battery powered sampling devices Sampling devices powered with integral batteries shall be provided with an early indication of low battery condition. Short recharge time, light-weight and low noise levels are recommended. 5.7 Airflow-control During the sampling deviation from the required airflow should not exceed ± 5 %. 5.8 Airflow-meter A device to measure the airflow through the sampling device before and after sampling in the field should be supplied if required. NOTE This device can be different from that used for calibration. SIST EN 14583:2004

EN 14583:2004 (E) 8 Sampling devices with integral airflow meters shall be calibrated against a traceable external airflow meter before use. 5.9 Labelling and marking The source and the manufacturer of the sampling device shall be clearly identified to ensure traceability to published performance characteristics. 5.10 Instruction manual The instruction manual shall be written in a language, which is understandable in the country of the operator. It shall be easily understood and every function explained. It shall illustrate all operation knobs and their handling by figures. It shall give the environmental and other conditions under which the instrument shall be operated including limitations to its use. It shall give exact calibration instructions and recommended equipment (e.g. flow meters) to be used. The manual shall also give the address for service of the instrument. 5.11 Fraction to be sampled To measure personal exposure to bioaerosols the sampling device should follow the criteria for size fractions of airborne particles according to EN 481. 5.12 Physical sampling efficiency Physical sampling efficiency is defined in EN 13098 and EN 13205. It shall be measured as a function of particle aerodynamic diameter and other influent parameters. NOTE 1 Annex A gives some information about the physical behaviour of a sampling device and the experimental assessment of its physical sampling efficiency in the laboratory. Physical sampling efficiency can be interpreted as the percentage of ambient particles of specific size that are sampled and collected by the sampling device. As airborne micro-organisms often adhere to non-biological particles of various sizes, the measurement of micro-organism concentration implies an accurate knowledge of this parameter. Sampling devices shall collect a representative sample of the required health related fraction of the bioaerosol (see EN 481). When assessing the performance of any bioaerosol sampling device, the overall sampling efficiency shall be determined. The performance of a personal sampler will be affected by the proximity of the operator's body when worn within the breathing zone, and therefore personal inhalable sampling devices need to be assessed whilst attached to a torso or other well characterised equivalents. NOTE 2 Personal sampling is performed when assessing the exposure to evaluate a suspected disease. Many biological tests are more effectively carried out with a personal sampler used as a free standing device. A free-standing sampler can be used to scan the environment for high emissions of micro-organisms. 5.13 Preservation efficiency Preservation efficiency is defined in EN 13098 as the capacity of the sampling device to maintain the culturability of the airborne micro-organisms during collection and also to keep the microbial products intact. The loss of culturability of micro-organisms due to sampling stress shall be tested on relevant model organisms. SIST EN 14583:2004

EN 14583:2004 (E) 9 5.14 Concentration range The manufacturer shall give the operational range and its limitations. An ideal sampling device should be able to sample concentrations of culturable micro-organisms and/or total number of micro-organisms up to 1010 m-3 air. 5.15 Duration of sampling The collected bioaerosol samples should refer to a time period representative of that

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