EN 17251:2020

Foodstuffs - Determination of ochratoxin A in pork meat and derived products by IAC clean-up and HPLC-FLD

This document describes a procedure for the determination of ochratoxin A (OTA) in pork products specifically ham, pork-based products (canned chopped pork) and pork liver using high performance liquid chromatography with fluorescence detection (HPLC-FLD).
The method has been validated for ochratoxin A in naturally contaminated ham, pork based products (canned chopped pork) and pork liver containing 0,5 μg/kg to 11 μg/kg [4], [5], [6].
Laboratory experiences have shown that this method is also applicable to pâté and kidney [4].

Lebensmittel - Bestimmung von Ochratoxin A in Schweinefleisch und Schweinefleischerzeugnissen mit Hochleistungsflüssigchromatographie und Fluoreszenzdetektion (HPLC-FLD)

Dieses Dokument beschreibt ein Verfahren zur Bestimmung von Ochratoxin A (OTA) in Schweinefleischerzeugnissen, insbesondere Schinken, Schweinefleisch-basierten Erzeugnissen (geschnittenes Schweinefleisch in Dosen) und Schweineleber mit Hochleistungsflüssigchromatographie mit Fluoreszenzdetektion (HPLC-FLD, en: high performance liquid chromatography with fluorescence detection).
Das Verfahren wurde für Ochratoxin A in natürlich kontaminiertem Schinken, Schweinefleisch-basierten Erzeugnissen (geschnittenem Schweinefleisch in Dosen) und Schweineleber im Bereich von 0,5 μg/kg bis 11 μg/kg validiert [4], [5], [6].
Laborerfahrungen haben gezeigt, dass dieses Verfahren auch auf Pastete und Nieren anwendbar ist [4].

Produits alimentaires - Dosage de l'ochratoxine A dans la viande de porc et les produits carnés issus du porc par chromatographie liquide à haute performance couplée à la détection par fluorescence (CLHP-DFL)

La présente Norme européenne décrit un mode opératoire de dosage de l’ochratoxine A (OTA) dans les produits issus du porc, notamment le jambon, les produits carnés à base de porc (porc haché en conserve) et le foie de porc, par chromatographie liquide à haute performance couplée à la détection par fluorescence (CLHP-DFL).
La méthode a été validée dans des échantillons naturellement contaminés en ochratoxine A de jambon, de produits carnés à base de porc (porc haché en conserve) et de foie de porc à des teneurs allant de 0,5 μg/kg à 11 μg/kg [4], [5], [6].
Les expériences en laboratoire ont démontré que cette méthode est également applicable aux pâtés et aux reins [4].

Živila - Določevanje ohratoksina A v svinjskem mesu in predelanih proizvodih z IAC-čiščenjem in tekočinsko kromatografijo visoke ločljivosti s fluorescenčno detekcijo (HPLC-FLD)

Ta dokument opisuje postopek za določevanje ohratoksina A (OTA) v proizvodih iz svinjskega mesa, natančneje v šunki, proizvodih, ki vsebujejo prašičje meso (sesekljana svinjina v konzervi), in svinjskih jetrih z visokozmogljivo tekočinsko kromatografijo s fluorescenčno detekcijo (HPLC-FLD).  Metoda je bila validirana za ohratoksin A z naravno kontaminirano šunko, proizvodi, ki vsebujejo prašičje meso (sesekljana svinjina v konzervi), in svinjskimi jetri z vsebnostjo 0,5 do 11 μg/kg [4, 5, 6]. Izkušnje v laboratoriju so pokazale, da je ta metoda uporabna tudi za pašteto in ledvice [4].

General Information

• Status: Published
• Publication Date: 14-Jan-2020
• Withdrawal Date: 30-Jul-2020
• ICS: 67.120.10 - Meat and meat products
• Technical Committee: CEN/TC 275 - Food analysis - Horizontal methods
• Drafting Committee: CEN/TC 275/WG 5 - Biotoxins
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 15-Jan-2020
• Due Date: 02-Nov-2019
• Completion Date: 15-Jan-2020
• Directive: 882/2004 - Regulation (EC) No 882/2004 of the European Parliament and of the Council of 29 April 2004 onofficial controls performed to ensure the verification of compliance with feed and food law, animal health and animal welfare rules
• Mandate: M/520 - Mandate for standardisation addressed to CEN for methods of analysis for mycotoxins in food
• Ref Project: SIST EN 17251:2020 - Foodstuffs - Determination of ochratoxin A in pork meat and derived products by IAC clean-up and HPLC-FLD

Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN 17251:2020
01-marec-2020
Živila - Določevanje ohratoksina A v svinjskem mesu in predelanih proizvodih z
IAC-čiščenjem in tekočinsko kromatografijo visoke ločljivosti s fluorescenčno
detekcijo (HPLC-FLD)
Foodstuffs - Determination of ochratoxin A in pork meat and derived products by IAC
clean-up and HPLC-FLD
Lebensmittel - Bestimmung von Ochratoxin A in Schweinefleisch und
Schweinefleischerzeugnissen mit Hochleistungsflüssigchromatographie und
Fluoreszenzdetektion (HPLC-FLD)
Produits alimentaires - Dosage de l'ochratoxine A dans la viande de porc et les produits
carnés issus du porc par chromatographie liquide à haute performance couplée à la
détection par fluorescence (CLHP-DFL)
Ta slovenski standard je istoveten z: EN 17251:2020
ICS:
67.120.10 Meso in mesni proizvodi Meat and meat products
SIST EN 17251:2020 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------
SIST EN 17251:2020

---------------------- Page: 2 ----------------------
SIST EN 17251:2020


EN 17251
EUROPEAN STANDARD

NORME EUROPÉENNE

January 2020
EUROPÄISCHE NORM
ICS 67.120.10
English Version

Foodstuffs - Determination of ochratoxin A in pork meat
and derived products by IAC clean-up and HPLC-FLD
Produits alimentaires - Dosage de l'ochratoxine A dans Lebensmittel - Bestimmung von Ochratoxin A in
la viande de porc et les produits carnés issus du porc Schweinefleisch und Schweinefleischerzeugnissen mit
par chromatographie liquide à haute performance Hochleistungsflüssigchromatographie und
couplée à la détection par fluorescence (CLHP-DFL) Fluoreszenzdetektion (HPLC-FLD)
This European Standard was approved by CEN on 6 October 2019.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.





EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2020 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 17251:2020 E
worldwide for CEN national Members.

---------------------- Page: 3 ----------------------
SIST EN 17251:2020
EN 17251:2020 (E)
Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative references . 5
3 Terms and definitions . 5
4 Principle . 5
5 Reagents . 5
6 Apparatus and equipment . 8
7 Procedure. 9
8 HPLC-FLD analysis .
...