EN ISO 15753:2016
(Main)Animal and vegetable fats and oils - Determination of polycyclic aromatic hydrocarbons (ISO 15753:2016)
Animal and vegetable fats and oils - Determination of polycyclic aromatic hydrocarbons (ISO 15753:2016)
ISO 15753:2016 describes two methods for the determination of 15 polycyclic aromatic hydrocarbons (PAHs) in animal and vegetable fats and oils:
- a general method;
- a specific method for coconut oil and vegetable oils with short-chain fatty acids.
These methods are not quantitative for the very volatile compounds such as naphthalene, acenaphthene and fluorene. Due to interferences provided by the matrix itself, palm oil and olive pomace oil cannot be analysed using this method.
The quantification limit is 0,2 µg/kg for almost all compounds analysed, except for fluoranthene and benzo(g,h,i)perylene, where the quantification limit is 0,3 µg/kg, and indeno(1,2,3-c,d)pyrene, where the quantification limit is 1,0 µg/kg.
NOTE The results for olive pomace oil in Annex B show that this method is not applicable to this type of oil. The precision data determined are very poor.
Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this International Standard.
Tierische und pflanzliche Fette und Öle - Bestimmung von polycyclischen aromatischen Kohlenwasserstoffen (ISO 15753:2016)
Diese Internationale Norm beschreibt zwei Verfahren zur Bestimmung von 15 polycyclischen aromatischen Kohlenwasserstoffen (PAKs) in tierischen und pflanzlichen Fetten und Ölen:
— ein allgemeines Verfahren;
— ein spezielles Verfahren für Kokosfett und Pflanzenöle mit kurzkettigen Fettsäuren.
Diese Verfahren sind bei leicht flüchtigen Verbindungen, wie z. B. Naphthalin, Acenaphthen und Fluoren nicht quantitativ. Aufgrund von Störungen, die durch die Matrix selbst bedingt sind, können Palmöl und Oliven¬tresteröl nicht mit diesem Verfahren analysiert werden.
Die Bestimmungsgrenze beträgt bei fast allen analysierten Verbindungen 0,2 μg/kg, mit Ausnahme von Fluoranthen und Benzo(g,h,i)perylen, deren Bestimmungsgrenze bei 0,3 μg/kg liegt, sowie Indeno(1,2,3 c,d)pyren mit einer Bestimmungsgrenze von 1,0 μg/kg.
ANMERKUNG Die Ergebnisse für Oliventresteröl in Anhang B zeigen, dass dieses Verfahren nicht für diese Ölart anwendbar ist. Die ermittelten Präzisionsdaten sind sehr schlecht.
Milch und Milcherzeugnisse (oder Fett aus Milch und Milcherzeugnissen) sind vom Anwendungsbereich dieser Internationalen Norm ausgenommen.
Corps gras d'origines animale et végétale - Détermination des hydrocarbures aromatiques polycycliques (ISO 15753:2016)
ISO 15753:2016 décrit deux méthodes pour la détermination de 15 hydrocarbures aromatiques polycycliques (HAP) dans les corps gras d'origines animale et végétale:
? une méthode générale;
? une méthode spécifique pour l'huile de coprah et les huiles végétales avec acides gras à chaînes courtes.
Ces méthodes ne sont pas quantitatives pour les composés très volatils tels que le naphtalène, l'acénaphtène et le fluorène. En raison des interférences causées par la matrice elle-même, l'huile de palme et l'huile de grignons d'olive ne peuvent pas être analysées par cette méthode.
La limite de quantification est de 0,2 µg/kg pour la majorité des composés analysés, à l'exception du fluoranthène et du benzo(g,h,i)pérylène, dont la limite de quantification est de 0,3 µg/kg, et de l'indéno(1,2,3-c,d)pyrène, dont la limite de quantification est de 1,0 µg/kg.
NOTE Les résultats pour l'huile de grignons d'olive dans l'Annexe B montrent que cette méthode n'est pas applicable à ce type d'huile. Les données de fidélité déterminées sont très médiocres.
Le lait et les produits laitiers (ou la graisse provenant du lait et des produits laitiers) sont exclus du domaine d'application de la présente Norme internationale.
Rastlinske in živalske maščobe in olja - Določevanje policikličnih aromatskih ogljikovodikov (ISO 15753:2016)
Ta mednarodni standard opisuje dve metodi za določevanje 15 policikličnih aromatskih
ogljikovodikov (PAH) v živalskih in rastlinskih maščobah in oljih:
– splošno metodo;
– posebno metodo za kokosovo olje in rastlinska olja z maščobnimi kislinami s kratko verigo.
Ti metodi nista kvantitativni za zelo hlapljive spojine, kot so naftalen, acenaften in fluoren. Zaradi motenj, ki jih povzroča sama matrika, s to metodo ni mogoče analizirati palmovega olja in olja iz oljčnih tropin.
Meja kvantifikacije je 0,2 μg/kg pri skoraj vseh analiziranih spojinah, razen fluorantena in benzo(g,h,i)perilena, kjer je meja kvantifikacije 0,3 μg/kg, ter indeno(1,2,3-c,d)pirena, kjer je meja kvantifikacije 1,0 μg/kg.
OPOMBA: Rezultati za olja iz oljčnih tropin iz dodatka B kažejo, da se ta metoda ne uporablja za to vrsto olja. Pridobljeni podatki o natančnosti so zelo slabi.
Mleko in mlečni izdelki (ali maščoba iz mleka in mlečnih izdelkov) niso zajeti v tem mednarodnem standardu.
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
01-junij-2016
1DGRPHãþD
SIST EN ISO 15753:2007
SIST EN ISO 15753:2007/A1:2011
5DVWOLQVNHLQåLYDOVNHPDãþREHLQROMD'RORþHYDQMHSROLFLNOLþQLKDURPDWVNLK
RJOMLNRYRGLNRY ,62
Animal and vegetable fats and oils - Determination of polycyclic aromatic hydrocarbons
(ISO 15753:2016)
Tierische und pflanzliche Fette und Öle - Bestimmung von polycyclischen aromatischen
Kohlenwasserstoffen (ISO 15753:2016)
Corps gras d'origines animale et végétale - Détermination des hydrocarbures
aromatiques polycycliques (ISO 15753:2016)
Ta slovenski standard je istoveten z: EN ISO 15753:2016
ICS:
67.200.10 5DVWOLQVNHLQåLYDOVNH Animal and vegetable fats
PDãþREHLQROMD and oils
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EN ISO 15753
EUROPEAN STANDARD
NORME EUROPÉENNE
April 2016
EUROPÄISCHE NORM
ICS 67.200.10 Supersedes EN ISO 15753:2006
English Version
Animal and vegetable fats and oils - Determination of
polycyclic aromatic hydrocarbons (ISO 15753:2016)
Corps gras d'origines animale et végétale - Tierische und pflanzliche Fette und Öle - Bestimmung
Détermination des hydrocarbures aromatiques von polycyclischen aromatischen Kohlenwasserstoffen
polycycliques (ISO 15753:2016) (ISO 15753:2016)
This European Standard was approved by CEN on 11 March 2016.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2016 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 15753:2016 E
worldwide for CEN national Members.
Contents Page
European foreword . 3
European foreword
This document (EN ISO 15753:2016) has been prepared by Technical Committee ISO/TC 34 “Food
products” in collaboration with Technical Committee CEN/TC 307 “Oilseeds, vegetable and animal fats
and oils and their by-products - Methods of sampling and analysis” the secretariat of which is held by
AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by October 2016, and conflicting national standards shall
be withdrawn at the latest by October 2016.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent
rights.
This document supersedes EN ISO 15753:2006.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta,
Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and the United Kingdom.
Endorsement notice
The text of ISO 15753:2016 has been approved by CEN as EN ISO 15753:2016 without any modification.
INTERNATIONAL ISO
STANDARD 15753
Second edition
2016-04-01
Animal and vegetable fats and oils —
Determination of polycyclic aromatic
hydrocarbons
Corps gras d’origines animale et végétale — Détermination des
hydrocarbures aromatiques polycycliques
Reference number
ISO 15753:2016(E)
©
ISO 2016
ISO 15753:2016(E)
© ISO 2016, Published in Switzerland
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form
or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior
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ii © ISO 2016 – All rights reserved
ISO 15753:2016(E)
Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Reagents and materials . 2
6 Apparatus . 3
7 Sampling . 4
8 Sample preparation . 5
9 Procedure for determination of PAHs from fats and oils: General method .5
9.1 Preliminary remarks . 5
9.2 Blank . 5
9.3 Determination of recovery values (without matrix) . 5
9.4 Extraction (liquid/liquid extraction) . 5
9.5 Purification on C18-bonded phase cartridge (solid/liquid extraction). 6
9.6 Purification on Florisil-bonded phase cartridge (solid/liquid extraction). 7
10 Procedure for determination of PAHs from fats and oils: Method specific for coconut oil .8
10.1 First extraction (liquid/liquid extraction) . 8
10.2 Second extraction (liquid/liquid extraction) . 8
10.3 Purification on C18-bonded phase cartridge (solid/liquid extraction). 8
10.4 Purification on Florisil-bonded phase cartridge (solid/liquid extraction). 9
11 High-performance liquid chromatography (HPLC) .10
11.1 Operating conditions .10
11.2 Detection parameters .10
11.3 Analysis of samples and standards .12
11.4 Confirmation of the presence of PAHs .12
12 Expression of results .12
13 Precision .13
13.1 Interlaboratory test.13
13.2 Repeatability .13
13.3 Reproducibility .13
14 Test report .13
Annex A (informative) Recovery values, flow charts, chromatograms and injection sequences .15
Annex B (informative) Results of the interlaboratory test .20
Bibliography .23
ISO 15753:2016(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
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ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www.iso.org/patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation on the meaning of ISO specific terms and expressions related to conformity
assessment, as well as information about ISO’s adherence to the WTO principles in the Technical
Barriers to Trade (TBT) see the following URL: Foreword - Supplementary information
The committee responsible for this document is ISO/TC 34, Food products, Subcommittee SC 11, Animal
and vegetable fats and oils.
This second edition cancels and replaces the first edition (ISO 15753:2006), of which it constitutes a
minor revision. It also incorporates Amendment ISO 15753:2006/Amd.1:2011. A non-applicability
statemen
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