EN ISO 22184:2021

Milk and milk products - Determination of the sugar contents - High performance anion exchange chromatography with pulsed amperometric detection method (HPAEC-PAD) (ISO 22184:2021)

This document specifies the quantitative liquid chromatographic determination of specific sugars (galactose, glucose, fructose, sucrose, lactose and maltose) in various milk and milk products, applying arabinose as an internal standard.
The method is applicable to the following dairy matrices: milk, sweetened condensed milk, milk powder, cheese, whey powder, infant formula, milk dessert and yoghurt.
The method does not apply to dairy products containing soy or to the determination of the lactose content in low-lactose milk products at levels below 1 mg/g.
A high performance anion exchange chromatography method in combination with pulsed amperometric detection (HPAEC-PAD) method is applied[5][3][4]. With this method, thirteen different monosaccharides, disaccharides and trisaccharides can be separated: fucose, arabinose, galactose, glucose, fructose, sucrose, lactose, lactulose, maltose, melibiose, trehalose, isomaltulose and maltotriose.
The method is applicable to labelling for the six most important sugars that can be present by nature or by addition in milk and milk products. The method does not apply to sugar contents less than 0,1 %.

Milch und Milcherzeugnisse - Bestimmung des Zuckergehalts - Verfahren der Hochleistungs-Anionenaustausch-Chromatographie mit gepulster amperometrischer Detektion (HPAEC PAD) (ISO 22184:2021)

Dieses Dokument legt die quantitative Bestimmung spezifischer Zucker (Galactose, Glucose, Fructose, Saccharose, Lactose und Maltose) in Milch und verschiedenen Milcherzeugnissen mittels Flüssigkeits-Chromatographie unter Anwendung von Arabinose als einen internen Standard fest.
Das Verfahren ist für die folgenden Molkerei-Matrizes anwendbar: Milch, gezuckerte Kondensmilch, Milchpulver, Käse, Molkenpulver, Säuglingsnahrung, Milchdessert und Joghurt.
Das Verfahren ist nicht für Molkereierzeugnisse, die Soja enthalten oder für die Bestimmung des Lactosegehalts in lactosearmen Milcherzeugnissen mit einem Gehalt unter 1 mg/g anwendbar.
Es wird ein Verfahren der Hochleistungs-Anionenaustausch-Chromatographie mit gepulster amperometrischer Detektion (HPAEC-PAD) angewendet [5][3][4]. Mit diesem Verfahren können dreizehn verschiedene Monosaccharide, Disaccharide und Trisaccharide getrennt werden: Fucose, Arabinose, Galactose, Glucose, Fructose, Saccharose, Lactose, Lactulose, Maltose, Melibiose, Trehalose, Isomaltulose und Maltotriose.
Das Verfahren ist für die Kennzeichnung der sechs wichtigsten Zucker anwendbar, die in Milch und Milcherzeugnissen natürlich oder durch Zusatz vorkommen können. Das Verfahren ist bei Zuckergehalten kleiner 0,1 % nicht anwendbar.

Lait et produits laitiers - Détermination de la teneur en sucre - Chromatographie d’échange d’anions haute performance couplée à la détection par ampérométrie pulsée (HPAEC-PAD) (ISO 22184:2021)

Le présent document spécifie la détermination quantitative par chromatographie en phase liquide de certains sucres (galactose, glucose, fructose, saccharose, lactose et maltose) dans divers laits et produits laitiers, en utilisant l'arabinose comme étalon interne.
Cette méthode s'applique aux matrices laitières suivantes: lait, lait concentré sucré, lait en poudre, fromage, lactosérum en poudre, formule infantile, dessert lacté et yaourt.
Cette méthode ne s'applique pas aux produits laitiers contenant du soja ni à la détermination de la teneur en lactose des produits laitiers à faible teneur en lactose inférieure à 0,1 mg/g.
Cette méthode s'appuie sur la chromatographie d'échange d'anions à haute performance couplée à la détection par ampérométrie pulsée (HPAEC-PAD)[5][3][4]. Treize monosaccharides, disaccharides et trisaccharides différents peuvent être séparés par cette méthode: fucose, arabinose, galactose, glucose, fructose, saccharose, lactose, lactulose, maltose, mélibiose, tréhalose, isomaltulose et maltotriose.
Cette méthode s'applique à l'étiquetage des six principaux sucres qui peuvent être présents naturellement ou ajoutés dans le lait et les produits laitiers. Cette méthode ne s'applique pas aux teneurs en sucre inférieures à 0,1 %.

Mleko in mlečni proizvodi - Določanje vsebnosti sladkorjev - Anionska izmenjevalna kromatografija z visoko ločljivostjo s pulzno amperometrično detekcijo (HPAEC-PAD) (ISO 22184:2021)

General Information

• Status: Published
• Publication Date: 16-Feb-2021
• Withdrawal Date: 30-Aug-2021
• ICS: 67.100.01 - Milk and milk products in general
• Technical Committee: CEN/TC 302 - Milk and milk products - Methods of sampling and analysis
• Drafting Committee: CEN/TC 302 - Milk and milk products - Methods of sampling and analysis
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 17-Feb-2021
• Completion Date: 17-Feb-2021
• Ref Project: SIST EN ISO 22184:2021 - Milk and milk products - Determination of the sugar contents - High performance anion exchange chromatography with pulsed amperometric detection method (HPAEC-PAD) (ISO 22184:2021)

Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN ISO 22184:2021
01-april-2021
Mleko in mlečni proizvodi - Določanje vsebnosti sladkorjev - Anionska
izmenjevalna kromatografija z visoko ločljivostjo s pulzno amperometrično
detekcijo (HPAEC-PAD) (ISO 22184:2021)
Milk and milk products - Determination of the sugar contents - High performance anion
exchange chromatography with pulsed amperometric detection method (HPAEC-PAD)
(ISO 22184:2021)
Milch und Milcherzeugnisse - Bestimmung des Zuckergehalts - Verfahren der
Hochleistungs-Anionenaustausch-Chromatographie mit gepulster amperometrischer
Detektion (HPAEC PAD) (ISO 22184:2021)
Lait et produits laitiers - Détermination de la teneur en sucre - Chromatographie
d’échange d’anions haute performance couplée à la détection par ampérométrie pulsée
(HPAEC-PAD) (ISO 22184:2021)
Ta slovenski standard je istoveten z: EN ISO 22184:2021
ICS:
67.100.01 Mleko in mlečni proizvodi na Milk and milk products in
splošno general
SIST EN ISO 22184:2021 en
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN ISO 22184:2021

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SIST EN ISO 22184:2021


EN ISO 22184
EUROPEAN STANDARD

NORME EUROPÉENNE

February 2021
EUROPÄISCHE NORM
ICS 67.100.01
English Version

Milk and milk products - Determination of the sugar
contents - High performance anion exchange
chromatography with pulsed amperometric detection
method (HPAEC-PAD) (ISO 22184:2021)
Lait et produits laitiers - Détermination de la teneur en Milch und Milcherzeugnisse - Bestimmung des
sucre - Chromatographie d'échange d'anions haute Zuckergehalts - Verfahren der Hochleistungs-
performance couplée à la détection par ampérométrie Anionenaustausch-Chromatographie mit gepulster
pulsée (HPAEC-PAD) (ISO 22184:2021) amperometrischer Detektion (HPAEC PAD) (ISO
22184:2021)
This European Standard was approved by CEN on 30 October 2020.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.





EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2021 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 22184:2021 E
worldwide for CEN national Members.

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SIST EN ISO 22184:2021
EN ISO 22184:2021 (E)
Contents Page
European foreword . 3

2

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SIST EN ISO 22184:2021
EN ISO 22184:2021 (E)
European foreword
This document (EN ISO 22184:2021) has been prepared by Technical Committee ISO/TC 34 "Food
products" in collaboration with Technical Committee CEN/TC 302 “Milk and milk products - Methods of
sampling and analysis” the secretariat of which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by August 2021, and conflicting national standards shall
be withdrawn at the latest by August 2021.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Ser
...