FprEN 17707

Plant biostimulants - Determination of the yeast and mould content

This document specifies a horizontal method for the enumeration of yeasts and moulds present in plant biostimulants intended for use in agriculture, by means of the colony count technique after aerobic incubation at 25 °C ± 2,5 °C.
This document allows the enumeration of yeasts and moulds, in technical and formulated plant biostimulant, both in liquid and solid state. The method is applicable to microbial plant biostimulant except those composed of fungi or yeast to verify that the concentration of yeast and moulds does not exceed the respective limits described in the EU Fertilisers Regulation [1].
If necessary, yeast and mould enumerated can be identified using suitable identification tests.

Pflanzen-Biostimulanzien - Bestimmung des Gehalts an Hefen und Schimmelpilzen

Dieses Dokument legt ein horizontales Verfahren zur Zählung von Hefen und Schimmelpilzen in Pflanzen-Biostimulanzien, die zur Verwendung in der Landwirtschaft bestimmt sind, mithilfe des Koloniezähl-verfahrens nach aerober Bebrütung bei (25 ± 2,5) °C fest.
Dieses Dokument hat die Zählung von Hefen und Schimmelpilzen in technischen und formulierten Pflanzen-Biostimulanzien, die im flüssigen oder im festen Zustand vorliegen können, zum Gegenstand. Das Verfahren ist auf mikrobielle Pflanzen-Biostimulanzien anwendbar, ausgenommen jene, die aus Pilzen oder Hefe bestehen, um zu überprüfen, dass die Konzentration von Hefen und Schimmelpilzen die jeweiligen Grenzwerte, die in der EU Düngeprodukteverordnung [1] angegeben sind, nicht überschreitet.
Gegebenenfalls können die gezählten Hefen und Schimmelpilze durch geeignete Identifizierungsprüfungen identifiziert werden.

Biostimulants des végétaux - Détermination de la teneur en levures et en moisissures

Le présent document spécifie une méthode horizontale pour le dénombrement des levures et des moisissures présentes dans les biostimulants des végétaux destinés à être utilisés en agriculture, par la technique de comptage des colonies après incubation aérobie à 25 °C ± 2,5 °C.
Le présent document permet le dénombrement des levures et des moisissures, dans les biostimulants des végétaux de qualité technique, à la fois à l’état liquide et à l’état solide. La méthode est applicable aux biostimulants microbiens des végétaux, excepté ceux composés de champignons ou de levures, pour vérifier que la concentration des levures et des moisissures ne dépasse pas les limites correspondantes indiquées dans le Règlement UE sur les fertilisants [1].
Si nécessaire, les levures et les moisissures dénombrées peuvent être identifiées par des essais d’identification appropriés.

Rastlinski biostimulanti - Določanje kvasovk in plesni

General Information

• Status: Not Published
• Publication Date: 18-Sep-2024
• ICS: 65.080 - Fertilizers
• Technical Committee: CEN/TC 455 - Plant Biostimulants and Agricultural Micro-Organisms
• Drafting Committee: CEN/TC 455/WG 3 - Pathogenic and non-pathogenic microorganisms
• Current Stage: 6055 - CEN Ratification completed (DOR) - Publishing
• Start Date: 26-Aug-2024
• Due Date: 12-Mar-2024
• Completion Date: 26-Aug-2024
• Directive: 2019/1009 - Regulation (EU) 2019/1009 of the European Parliament and of the Council of 5 June 2019 laying down rules on the making available on the market of EU fertilising products and amending Regulations (EC) No 1069/2009 and (EC) No 1107/2009 and repealing Regulation (EC) No 2003/2003
• Mandate: M/564 - Fertilising products in support of Regulation (EU) 2019/1009
• Mandate: M/564 AMD 1 - amending Implementing Decision C(2020) 612 as regards certain harmonised standards and standardisation deliverables, deadlines for their adoption and the duration of the validity of the standardisation request
• Mandate: M/XXX - COMMISSION IMPLEMENTING DECISION of XXX on a standardisation request to the European Committee for Standardisation as regards methane emissions quantification and mitigation in support of Regulation (EU) 2024/1787 of the European Parliament and of the Council on the reduction of methane emissions in the energy sector and amending Regulation (EU) 2019/942
• Ref Project: kSIST FprEN 17707:2024 - Plant biostimulants - Determination of the yeast and mould content

Relations

• Revises: CEN/TS 17707:2022 - Plant biostimulants - Determination of the yeast and mould content
• Effective Date: 15-Feb-2023

Standards Content (Sample)

SLOVENSKI STANDARD
oSIST prEN 17707:2023
01-maj-2023
Rastlinski biostimulanti - Ugotavljanje prisotnosti in števila kvasovk in plesni
Plant biostimulants - Determination of the yeast and mould content
Pflanzen-Biostimulanzien - Bestimmung des Gehalts an Hefen und Schimmelpilzen
Biostimulants des végétaux - Détermination de la teneur en levures et en moisissures
Ta slovenski standard je istoveten z: prEN 17707
ICS:
65.080 Gnojila Fertilizers
oSIST prEN 17707:2023 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

oSIST prEN 17707:2023
oSIST prEN 17707:2023
DRAFT
EUROPEAN STANDARD
prEN 17707
NORME EUROPÉENNE
EUROPÄISCHE NORM
April 2023
ICS 65.080 Will supersede CEN/TS 17707:2022
English Version
Plant biostimulants - Determination of the yeast and
mould content
Biostimulants des végétaux - Détermination de la Pflanzen-Biostimulanzien - Bestimmung des Gehalts an
teneur en levures et en moisissures Hefen und Schimmelpilzen
This draft European Standard is submitted to CEN members for enquiry. It has been drawn up by the Technical Committee
CEN/TC 455.
If this draft becomes a European Standard, CEN members are bound to comply with the CEN/CENELEC Internal Regulations
which stipulate the conditions for giving this European Standard the status of a national standard without any alteration.

This draft European Standard was established by CEN in three official versions (English, French, German). A version in any other
language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC
Management Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
Recipients of this draft are invited to submit, with their comments, notification of any relevant patent rights of which they are
aware and to provide supporting documentation.

Warning : This document is not a European Standard. It is distributed for review and comments. It is subject to change without
notice and shall not be referred to as a European Standard.

EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2023 CEN All rights of exploitation in any form and by any means reserved Ref. No. prEN 17707:2023 E
worldwide for CEN national Members.

oSIST prEN 17707:2023
prEN 17707:2023 (E)
Contents Page
European foreword . 4
Introduction . 5
1 Scope . 7
2 Normative references . 7
3 Terms and definitions . 7
4 Principles . 8
4.1 General . 8
4.2 Plate count method . 8
5 Diluent and culture media . 8
5.1 General . 8
5.2 Diluent . 8
5.3 Culture media . 9
6 Apparatus and glassware . 9
7 Handling of plant biostimulants and sampling . 9
8 Procedure . 9
8.1 General . 9
8.2 Test portion and initial suspension . 9
8.2.1 General . 9
8.2.2 Liquid - water based - formulations . 9
8.2.3 Liquid – oil based (emulsifiable concentrate - EC) formulations . 10
8.2.4 Solid - Wettable Powder (WP) formulations . 10
8.2.5 Solid - Water dispersible granules (WDG) formulations . 10
8.2.6 Solid – Pellets, granules, microgranules (slow release) formulations . 10
8.2.7 Solid - substrate . 10
8.3 Serial dilutions . 10
8.4 Plate-count methods . 10
8.4.1 Pour-plate method . 10
8.4.2 Surface spread method . 11
8.4.3 Incubation . 11
9 Counting of colonies . 11
10 Expression of results . 12
10.1 Method of calculation . 12
10.2 Interpretation . 13
11 Test report . 13
Annex A (informative) Diluent . 15
A.1 General . 15
A.2 Phosphate Buffered Saline (PBS). 15
A.2.1 Composition . 15
A.2.2 Preparation . 15
Annex B (normative) Culture media. 16
oSIST prEN 17707:2023
prEN 17707:2023 (E)
B.1 General . 16
B.2 Sabouraud dextrose chloramphenicol agar medium (SDCA) . 16
B.2.1 Composition . 16
B.2.2 Preparation . 16
B.3 Potato dextrose agar (PDA) medium with antibiotics . 17
B.3.1 Composition . 17
B.3.2 Preparation . 17
B.4 Glucose -peptone (GP) agar medium with antibiotics . 17
B.4.1 Composition . 17
B.4.2 Preparation . 17
B.5 Malt extract agar (MEA) medium with antibiotics . 18
B.5.1 Composition . 18
B.5.2 Preparation . 18
Annex C (informative) Interlaboratory study . 19
C.1 Materials used in the interlaboratory study . 19
C.2 Results of the interlaboratory study . 20
Annex ZA (informative) Relationship of this European Standard and the essential
requirements of Regulation (EU) 2019/1009 making available on the market of EU
fertilising products aimed to be covered . 22
Bibliography . 23

oSIST prEN 17707:2023
prEN 17707:2023 (E)
European foreword
This document (prEN 17707:2023) has been prepared by Technical Committee CEN/TC 455
“Plant Biostimulants”, the secretariat of which is held by AFNOR.
This document is currently submitted to the CEN Enquiry.
This document will supersede CEN/TS 17707:2022.
This document has been prepared under a Standardization Request given to CEN by the European
Commission and the European Free Trade Association, and supports essential requirements of EU
Directive(s) / Regulation(s).
For relationship with EU Directive(s) / Regulation(s), see informative Annex ZA, which is an
integral part of this document.

oSIST prEN 17707:2023
prEN 17707:2023 (E)
Introduction
This document was prepared by the experts of CEN/TC 455 “Plant Biostimulants”. The European
Committee for Standardization (CEN) was requested by the European Commission (EC) to draft
European standards or European standardization deliverables to support the implementation of
Regulation (EU) 2019/1009 of 5 June 2019 laying down rules on the making available on the
market of EU fertilizing products (“FPR” or “Fertilising Products Regulation”).
This standardization request, presented as SR M/564 and M/564 Amd1, also contributes to the
Communication on “Innovating for Sustainable Growth: A Bio economy for Europe”. The Working
Group 5 “Labelling and denominations”, was created to develop a work program as part of this
request. The technical committee CEN/TC 455 “Plant Biostimulants” was established to carry out
the work program that will prepare a series of standards. The interest in biostimulants has
increased significantly in Europe as a valuable tool to use in agriculture. Standardization was
identified as having an important role in order to promote the use of biostimulants. The work of
CEN/TC 455 seeks to improve the reliability of the supply chain, thereby improving the confidence
of farmers, industry, and consumers in biostimulants, and will promote and support
commercialisation of the European biostimulant industry.
Biostimulants used in agriculture can be applied in multiple ways: on soil, on plant, as seed
treatment, etc. A microbial plant biostimulant consists of a microorganism or a consortium of
microorganisms, as referred to in Component Material Category 7 of Annex II of the EU Fertilising
Products Regulation.
This document is applicable to all microbial biostimulants in agriculture.
Table 1 below summarizes many of the agro-ecological principles and the role played by
biostimulants.
Table 1 — Agro-ecological principles and the role played by biostimulants [1]
Increase biodiversity
By improving soil microorganism quality/quantity
Reinforce biological regulation and interactions
By reinforcing plant-microorganism interactions
- symbiotic exchanges i.e. mycorrhize
- symbiotic exchanges i.e. rhizobiaciae/fava
- secretions mimicking plant hormones (i.e. trichoderma)
By regulating plant physiological processes
- for ex growth, metabolism, plant development…
Improve bio
...