EN ISO 21149:2017/A1:2022

Cosmetics - Microbiology - Enumeration and detection of aerobic mesophilic bacteria - Amendment 1 (ISO 21149:2017/Amd 1:2022)

Kosmetische Mittel - Mikrobiologie - Zählung und Nachweis von aeroben mesophilen Bakterien - Änderung 1 (ISO 21149:2017/Amd 1:2022)

No scope available

Cosmétiques - Microbiologie - Dénombrement et détection des bactéries aérobies mésophiles - Amendement 1 (ISO 21149:2017/Amd 1:2022)

Kozmetika - Mikrobiologija - Ugotavljanje prisotnosti in števila aerobnih mezofilnih bakterij - Dopolnilo A1 (ISO 21149:2017/Amd 1:2022)

General Information

• Status: Published
• Publication Date: 27-Sep-2022
• ICS: 07.100.40 - Cosmetics microbiology
• Technical Committee: CEN/TC 392 - Cosmetics
• Drafting Committee: CEN/TC 392 - Cosmetics
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 28-Sep-2022
• Completion Date: 28-Sep-2022
• Ref Project: SIST EN ISO 21149:2017/A1:2023 - Cosmetics - Microbiology - Enumeration and detection of aerobic mesophilic bacteria - Amendment 1 (ISO 21149:2017/Amd 1:2022)

Relations

• Amends: EN ISO 21149:2017 - Cosmetics - Microbiology - Enumeration and detection of aerobic mesophilic bacteria (ISO 21149:2017)
• Effective Date: 22-Sep-2021

Overview

EN ISO 21149:2017/A1:2022 is Amendment 1 to ISO 21149:2017, adopted by CEN as EN ISO 21149:2017/A1:2022. The standard addresses cosmetics microbiology - enumeration and detection of aerobic mesophilic bacteria. The amendment updates media and preparation details for enrichment and agar media used in the recovery and enumeration of aerobic mesophilic bacteria from cosmetic products and related samples.

Key topics and requirements

• Scope: Procedures for detection and enumeration of aerobic mesophilic bacteria in cosmetics (as part of ISO 21149 method family).
• Enrichment broth: Amendment clarifies subclause titles and details for enrichment media.
  • Eugon LT100 broth: Described with neutralizers (lecithin, polysorbate 80) and a dispersing agent (octoxynol 9).
  • Modified Eugon LT broth (new): Specification of composition per 1 000 ml:
    • Pancreatic digest of casein 15 g
    • Papain (papaic) digest of soybean meal 5 g
    • Sodium chloride 4 g
    • L-cystine 0.7 g
    • Sodium sulphite 0.2 g
    • Glucose 5.5 g
    • Egg lecithin 1 g
    • Polysorbate 80 15 g
    • Sodium lauryl ether sulfate 1.56 g
    • Water to 1 000 ml
  • Preparation: Dissolve polysorbate 80 and egg lecithin successively into boiling water; dissolve other components while heating; sterilize at 121 °C for 15 min; mix while hot; final pH 7.0 ± 0.2 at room temperature.
• Agar media: New Modified Eugon LT agar composition mirrors the broth with added agar (15 g per 1 000 ml) and identical preparation/sterilization instructions.
• Document control: Amendment was approved 10 September 2022; CEN endorsed it without modification and member bodies were to implement by March 2023.

Applications

• Routine microbial quality control (QC) of cosmetic raw materials, in-process samples and finished products.
• Validation and verification of preservative efficacy and microbial limit testing.
• Development and standardization of laboratory methods for cosmetic microbiology (culture-based enumeration and detection).

Who should use this standard

• Cosmetic industry microbiologists and QC laboratories
• Contract testing laboratories performing cosmetic microbial analyses
• Regulatory authorities and auditors assessing compliance with microbial safety requirements
• Standards bodies and method developers in cosmetics microbiology

Related standards

• ISO 21149:2017 (base standard) - Amendment 1 (ISO 21149:2017/Amd 1:2022) incorporated as EN ISO 21149:2017/A1:2022.
• Developed by ISO/TC 217 and CEN/TC 392 (cosmetics).

Keywords: ISO 21149, EN ISO 21149, cosmetics microbiology, aerobic mesophilic bacteria, Eugon LT broth, modified Eugon LT agar, microbial enumeration, cosmetic QC, culture media.

Standards Content (Sample)

SLOVENSKI STANDARD
01-april-2023
Kozmetika - Mikrobiologija - Ugotavljanje prisotnosti in števila aerobnih mezofilnih
bakterij - Dopolnilo A1 (ISO 21149:2017/Amd 1:2022)
Cosmetics - Microbiology - Enumeration and detection of aerobic mesophilic bacteria -
Amendment 1 (ISO 21149:2017/Amd 1:2022)
Kosmetische Mittel - Mikrobiologie - Zählung und Nachweis von aeroben mesophilen
Bakterien - Änderung 1 (ISO 21149:2017/Amd 1:2022)
Cosmétiques - Microbiologie - Dénombrement et détection des bactéries aérobies
mésophiles - Amendement 1 (ISO 21149:2017/Amd 1:2022)
Ta slovenski standard je istoveten z: EN ISO 21149:2017/A1:2022
ICS:
07.100.40 Kozmetika - mikrobiologija Cosmetics microbiology
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 21149:2017/A1
EUROPEAN STANDARD
NORME EUROPÉENNE
September 2022
EUROPÄISCHE NORM
ICS 07.100.40
English Version
Cosmetics - Microbiology - Enumeration and detection of
aerobic mesophilic bacteria - Amendment 1 (ISO
21149:2017/Amd 1:2022)
Cosmétiques - Microbiologie - Dénombrement et Kosmetische Mittel - Mikrobiologie - Zählung und
détection des bactéries aérobies mésophiles - Nachweis von aeroben mesophilen Bakterien -
Amendement 1 (ISO 21149:2017/Amd 1:2022) Änderung 1 (ISO 21149:2017/Amd 1:2022)
This amendment A1 modifies the European Standard EN ISO 21149:2017; it was approved by CEN on 10 September 2022.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for inclusion of
this amendment into the relevant national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This amendment exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the
same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2022 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 21149:2017/A1:2022 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
This document (EN ISO 21149:2017/A1:2022) has been prepared by Technical Committee ISO/TC 217
"Cosmetics" in collaboration with Technical Committee CEN/TC 392 “Cosmetics” the secretariat of
which is held by AFNOR.
This Amendment to the European Standard EN ISO 21149:2017 shall be given the status of a national
standard, either by publication of an identical text or by endorsement, at the latest by March 2023, and
conflicting national standards shall be withdrawn at the latest by March 2023.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
Any feedback and questions on this document should be directed to the users’ national standards
body/national committee. A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the
United Kingdom.
Endorsement notice
The text of ISO 21149:2017/Amd 1:2022 has been approved by CEN as EN ISO 21149:2017/A1:2022
without any modification.
INTERNATIONAL ISO
STANDARD 21149
Second edition
2017-06
AMENDMENT 1
2022-08
Cosmetics — Microbiology —
Enumeration and detection of aerobic
mesophilic bacteria
AMENDMENT 1
Cosmétiques — Microbiologie — Dénombrement et détection des
bactéries aérobies mésophiles
AMENDEMENT 1
Reference number
ISO 21149:2017/Amd.1:2022(E)
ISO 21149:2017/Amd.1:2022(E)
© ISO 2022
All rights reserved. Unless otherwise specified, or required in the context of
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