Wood preservatives - Determination of the eradicant action against Hylotrupes bajulus (Linnaeus) larvae - Laboratory method

This document specifies a method for the determination of the eradicant action of a surface application of a fast and a slow acting wood preservative product or a deferred acting wood preservative product on solid wood infested with larvae of Hylotrupes bajulus (Linnaeus).
This method is applicable to:
-   organic formulations, as supplied or as prepared in the laboratory by dilution of concentrates; or
-   organic water-dispersible formulations, as supplied or as prepared in the laboratory by dilution of concentrates; or
-   water-soluble products, for example, salts.
NOTE   An ageing procedure cannot be combined with this method.

Holzschutzmittel - Bestimmung der bekämpfenden Wirkung gegenüber Larven von Hylotrupes bajulus (Linnaeus) - Laboratoriumsverfahren

Dieses Dokument legt ein Verfahren zur Bestimmung der bekämpfenden Wirkung eines schnell und eines langsam wirkenden Holzschutzmittels oder eines Holzschutzmittels mit verzögerter Wirkung bei der Behandlung der Oberfläche von Vollholz fest, das von Larven des Hylotrupes bajulus (Linnaeus) befallen ist.
Das Verfahren ist anwendbar für:
-   organische Zubereitungen im Lieferzustand oder wie sie im Laboratorium durch Verdünnung von Konzentraten hergestellt werden; oder
-   organische in Wasser dispergierbare Zubereitungen im Lieferzustand oder wie sie im Laboratorium durch Verdünnung von Konzentraten hergestellt werden; oder
-   wasserlösliche Produkte, z. B. Salze.
ANMERKUNG   Ein Alterungsverfahren kann mit diesem Verfahren nicht kombiniert werden.

Produits de préservation du bois - Détermination de l'action curative contre les larves d'Hylotrupes bajulus (Linnaeus) - Méthode de laboratoire

Le présent document prescrit une méthode de détermination de l'efficacité curative d'un produit de préservation du bois à action rapide ou à action lente, ou d'un produit de préservation du bois à action différée, appliqué par un traitement de surface, sur des pièces de bois massif infestées par des larves d'Hylotrupes bajulus (Linnaeus).
La présente méthode est applicable :
-   soit aux formules organiques telles qu'elles sont fournies ou préparées au laboratoire par dilution de concentrés ;
-   soit aux formules organiques hydrodispersables, telles qu'elles sont fournies ou préparées au laboratoire par dilution de concentrés ;
-   soit aux produits hydrosolubles tels que les sels.
NOTE   Une épreuve de vieillissement ne peut pas être combinée avec cette méthode.

Zaščitna sredstva za les - Ugotavljanje učinkovitosti zatiranja ličink hišnega kozlička Hylotrupes bajulus (Linnaeus) - Laboratorijska metoda

General Information

Status
Published
Publication Date
07-Apr-2020
Current Stage
6060 - Definitive text made available (DAV) - Publishing
Due Date
08-Apr-2020
Completion Date
08-Apr-2020

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SLOVENSKI STANDARD
SIST EN 1390:2020
01-junij-2020
Nadomešča:
SIST EN 1390:2006
Zaščitna sredstva za les - Ugotavljanje učinkovitosti zatiranja ličink hišnega
kozlička Hylotrupes bajulus (Linnaeus) - Laboratorijska metoda

Wood preservatives - Determination of the eradicant action against Hylotrupes bajulus

(Linnaeus) larvae - Laboratory method
Holzschutzmittel - Bestimmung der bekämpfenden Wirkung gegenüber Larven von
Hylotrupes bajulus (Linnaeus) - Laboratoriumsverfahren

Produits de préservation du bois - Détermination de l'action curative contre les larves

d'Hylotrupes bajulus (Linnaeus) - Méthode de laboratoire
Ta slovenski standard je istoveten z: EN 1390:2020
ICS:
71.100.50 Kemikalije za zaščito lesa Wood-protecting chemicals
SIST EN 1390:2020 en,fr,de

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN 1390:2020
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SIST EN 1390:2020
EN 1390
EUROPEAN STANDARD
NORME EUROPÉENNE
April 2020
EUROPÄISCHE NORM
ICS 71.100.50 Supersedes EN 1390:2006
English Version
Wood preservatives - Determination of the eradicant
action against Hylotrupes bajulus (Linnaeus) larvae -
Laboratory method

Produits de préservation du bois - Détermination de Holzschutzmittel - Bestimmung der bekämpfenden

l'action curative contre les larves d'Hylotrupes bajulus Wirkung gegenüber Larven von Hylotrupes bajulus

(Linnaeus) - Méthode de laboratoire (Linnaeus) - Laboratoriumsverfahren
This European Standard was approved by CEN on 24 February 2020.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this

European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references

concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN

member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by

translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management

Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,

Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,

Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and

United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels

© 2020 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 1390:2020 E

worldwide for CEN national Members.
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SIST EN 1390:2020
EN 1390:2020 (E)
Contents Page

European foreword ............................................................................................................................... 4

Introduction ............................................................................................................................................. 5

1 Scope ............................................................................................................................................ 6

2 Normative references ............................................................................................................ 6

3 Terms and definitions ............................................................................................................ 6

4 Principle ...................................................................................................................................... 7

5 Test materials ........................................................................................................................... 7

5.1 Biological material .................................................................................................................. 7

5.1.1 Hylotrupes bajulus (Linnaeus) larvae............................................................................... 7

5.1.2 Source of larvae ........................................................................................................................ 7

5.1.3 Provision of larvae .................................................................................................................. 7

5.1.4 Choice of larvae ........................................................................................................................ 7

5.2 Products and reagents ........................................................................................................... 8

5.3 Apparatus ................................................................................................................................... 8

6 Sampling ..................................................................................................................................... 9

7 Test specimens ......................................................................................................................... 9

7.1 Species of wood ........................................................................................................................ 9

7.2 Wood quality ............................................................................................................................. 9

7.3 Provision of test specimens ................................................................................................. 9

7.4 Dimensions of test specimens ........................................................................................... 10

7.5 Number of test specimens .................................................................................................. 10

7.5.1 For fast and slow acting wood preservatives, test duration 12 weeks or 24

weeks ......................................................................................................................................... 10

7.5.2 For deferred acting wood preservatives, test duration 52 weeks ....................... 10

8 Procedure ................................................................................................................................. 10

8.1 Preparation of the test specimens ................................................................................... 10

8.2 Insertion of larvae into the test specimens .................................................................. 12

8.3 Sealing of the surfaces not to be treated ....................................................................... 12

8.4 Incubation of the test specimens ..................................................................................... 12

8.5 Treatment of test specimens ............................................................................................. 12

8.5.1 Preparation of the treatment solution ........................................................................... 12

8.5.2 Application of the treatment solution ............................................................................ 13

8.6 Drying of the test specimens ............................................................................................. 13

8.7 Duration of test ....................................................................................................................... 14

8.8 Examination of the test specimens .................................................................................. 14

8.8.1 Examination without radiography .................................................................................. 14

8.8.2 Examination with radiography (optional) ................................................................... 15

8.8.3 Expression of examination ................................................................................................. 15

9 Validity of the test .................................................................................................................. 15

10 Expression of results ............................................................................................................ 15

11 Test report ............................................................................................................................... 16

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Annex A (informative) Example of a test report ........................................................................ 17

Annex B (informative) Technique for culturing Hylotrupes bajulus (Linnaeus) ........... 19

B.1 General ...................................................................................................................................... 19

B.2 Obtaining parent beetles .................................................................................................... 19

B.3 Mating ........................................................................................................................................ 19

B.4 Egg-laying ................................................................................................................................. 19

B.5 Hatching of eggs ..................................................................................................................... 20

B.6 Larval development .............................................................................................................. 20

B.7 Enemies and parasites ......................................................................................................... 21

Annex C (informative) Environmental, health and safety precautions within

chemical/biological laboratory ........................................................................................ 22

Bibliography .......................................................................................................................................... 23

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SIST EN 1390:2020
EN 1390:2020 (E)
European foreword
This document (EN 1390:2020) has been prepared by Technical Committee CEN/TC 38

“Durability of wood and wood-based products”, the secretariat of which is held by AFNOR.

This European Standard shall be given the status of a national standard, either by publication of

an identical text or by endorsement, at the latest by October 2020, and conflicting national

standards shall be withdrawn at the latest by October 2020.

Attention is drawn to the possibility that some of the elements of this document may be the subject

of patent rights. CEN shall not be held responsible for identifying any or all such patent rights.

This document supersedes EN 1390:2006.

Significant technical differences between this document and EN 1390:2006 are as follows:

a) introduction of a definition for moribund larvae;

b) because of the changes in a) verification of the conditions of recovered larvae becomes

needless and was deleted;

c) alternative coatings for sealing surfaces not to be treated may be used if they have no harmful

effect to the test insects and if their performance as sealants has been documented;

d) the following note was added to 8.8 “Examination of the test specimens”: It is useful to record

the location (distance from the nearest treated surface) of all larvae recovered because it

provides valuable information on penetration characteristic of the preservative.

NOTE Test results obtained according to earlier versions of this document and when the tests had

started before this version of EN 1390 was published are considered as valid.

According to the CEN-CENELEC Internal Regulations, the national standards organisations of the

following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,

Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary,

Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland,

Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden,

Switzerland, Turkey and the United Kingdom.
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SIST EN 1390:2020
EN 1390:2020 (E)
Introduction

This document describes a laboratory method of testing which gives a basis for the assessment of

the eradicant action of fast and slow acting wood preservatives and of deferred acting wood

preservatives against Hylotrupes bajulus. It allows determination of the lethal effect of a surface

application of a preservative product on a population of larvae previously introduced into the test

specimens.

The method simulates conditions in practice where a beam is treated, which is only slightly

attacked and where cutting away has not exposed insect tunnels. This represents a severe test of

the product.

In some particular instances, for example where the preservative is to be used on timbers of large

dimensions, laminated beams, blockboard, plywood and other panel products, other test methods

can be used to obtain complementary information on the effectiveness of the eradicant action of

a product. Such methods lie outside the scope of this document.

This laboratory method provides one criterion by which the value of a product can be assessed.

In making this assessment the methods by which the preservative may be applied should be taken

into account. It is further recommended that results from this test should be supplemented by

those from other appropriate tests, and above all by comparison with practical experience.

When products that are very active at low concentrations are used it is very important to take

suitable precautions to isolate and separate, as far as possible, operations involving chemical

products, other products, treated wood, laboratory apparatus and clothing. Suitable precautions

should include the use of separate rooms, areas within rooms, extraction facilities, conditioning

chambers and special training for personnel.
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SIST EN 1390:2020
EN 1390:2020 (E)
1 Scope

This document specifies a method for the determination of the eradicant action of a surface

application of a fast and a slow acting wood preservative product or a deferred acting wood

preservative product on solid wood infested with larvae of Hylotrupes bajulus (Linnaeus).

This method is applicable to:

— organic formulations, as supplied or as prepared in the laboratory by dilution of concentrates;

— organic water-dispersible formulations, as supplied or as prepared in the laboratory by

dilution of concentrates; or
— water-soluble products, for example, salts.
NOTE An ageing procedure cannot be combined with this method.
2 Normative references

The following documents are referred to in the text in such a way that some or all of their content

constitutes requirements of this document. For dated references, only the edition cited applies.

For undated references, the latest edition of the referenced document (including any

amendments) applies.

EN ISO 3696, Water for analytical laboratory use — Specification and test methods (ISO 3696)

ISO 835:2007, Laboratory glassware — Graduated pipettes
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.

ISO and IEC maintain terminological databases for use in standardization at the following

addresses:
— IEC Electropedia: available at http://www.electropedia.org/
— ISO Online browsing platform: available at https://www.iso.org/obp/ui
3.1
moribund larva

larva that is in the state of inactivity or obsolescence due to exposure to the test product but which

are considered alive after a specific test period
3.2
representative sample

sample with physical and/or chemical characteristics identical to the volumetric average

characteristics of the total volume being sampled
[SOURCE: EN 1001-2:2005, 4.71]
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EN 1390:2020 (E)
3.3
supplier

sponsor of the test (person or company providing the sample of wood preservative to be tested)

[SOURCE: Adapted from EN 1001-2:2005, 4.83]
4 Principle

Insertion of larvae of Hylotrupes bajulus into test specimens. After a period of time to allow the

larvae to establish themselves in the test specimens, treatment of these test specimens by

brushing or pipetting of the test preservative product.

After the time defined by the speed of action of the wood preservative, assessment of the mortality

of the larvae compared with that of larvae in untreated control test specimens.
5 Test materials
5.1 Biological material
5.1.1 Hylotrupes bajulus (Linnaeus) larvae
5.1.2 Source of larvae

The larvae shall preferably be obtained from cultures reared according to the method described

in Annex B.

Larvae can also be taken from naturally infested wood, in which case they should be transferred

into sapwood of pine and stored for at least four weeks under the rearing conditions specified in

Annex B.
5.1.3 Provision of larvae
Carefully split or crumble infested blocks to extract larvae.

Keep the larvae separate from one another in glass receptacles for two or three days in the

culturing chamber (5.3.1) to check they are healthy.
5.1.4 Choice of larvae
Use only healthy larvae in the test.

NOTE A healthy larva can be recognized by ivory-white colour, its firm consistency and rounded

appearance, and by the absence of wounds or bites, which show up as dark marks. Healthy larvae react to

the touch by vigorous movement and attempts to bite.

Reject any larvae, which are shrunken or aged which have recently moulted, or which are in a pre-

pupal stage.

Weigh each larva and place it in a glass receptacle marking the receptacle with the weight of the

larva. Make up two groups with the weight ranges:
— 51 mg to 100 mg, and
— 101 mg to 150 mg.

Larvae with a mass larger than 150 mg in mass are unsuitable as they can pupate during the

course of the test.
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EN 1390:2020 (E)
5.2 Products and reagents

5.2.1 Paraffin wax, for sealing the relevant surfaces of specimens to be treated with solutions

in which water is the continuous phase.

NOTE Paraffin wax with a setting point of 52 °C to 54 °C has been found suitable.

5.2.2 Gelatine, for sealing the relevant surfaces of specimens to be treated with solutions in

which an organic solvent is the continuous phase.
5.2.3 Water, complying with grade 3 of EN ISO 3696.

5.2.4 Solvent or diluent, a volatile liquid that will dissolve or dilute the preservative but does

not leave a residue in the wood at the end of the post-treatment conditioning period that has a

toxic effect on the insects.
CAUTION — Do not use benzene or other solvents which pose a health risk.
5.3 Apparatus

5.3.1 Culturing chamber, with air circulation, controlled at (28 ± 2) °C and at a relative

humidity of (70 ± 5) %.

5.3.2 Ventilated fume cupboard, in which the specimens are treated with an input air

temperature of (20 ± 5) °C.

The maximum air speed, measured at the input opening with the sash in the approximate

operation position should be approx. 0,5 m/s.

CAUTION — It is essential to follow safety procedures for handling flammable and toxic materials.

Avoid excessive exposure of operators to solvents or their vapours.

5.3.3 Testing chamber, ventilated and controlled at (21 ± 2) °C and at a relative humidity of

(75 ± 5) %.
5.3.4 Drill and twist drills, with 3 mm, 4 mm and 5 mm diameters.

5.3.5 Pipettes as specified in ISO 835:2007, Class B - graduated pipette with no waiting time,

with a capacity 5 ml and an accuracy of ±0,05 ml.

5.3.6 Brush of appropriate size, to be used when the test substance is applied by brushing

(8.5.2).

5.3.7 Safety equipment and protective clothing, appropriate for the test product and the test,

to ensure the safety of the operator (see also Annex C).

5.3.8 Ordinary laboratory equipment, including a balance capable of weighing to an accuracy

of ±1 mg.

5.3.9 Rectangular cover with sides, constructed either of glass, plastics, plywood and of a

height not less than 200 mm and with an open face of sufficient size to cover all the treated

specimens from a single test.
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EN 1390:2020 (E)

5.3.10 X-ray apparatus (optional) with tungsten target and beryllium window, with voltage

and current continuously variable in the ranges:
— voltage: 10 kV to 50 kV;
— current: 0 mA to 15 mA.
5.3.11 Protective gloves.
6 Sampling

The sample of preservative shall represent the product to be tested. Samples shall be stored and

handled in accordance with any written recommendations from the supplier.

For the sampling of preservatives from bulk supplies, the procedure given in EN 212 should be

used.
7 Test specimens
7.1 Species of wood
The reference species is Scots pine (Pinus sylvestris Linnaeus).

NOTE In southern European countries the species of pine most frequently infested by Hylotrupes

bajulus can be used as an alternative, provided that the suitability of the species for use in the tests specified

in this standard has been demonstrated in all aspects (development of larvae, resistance to impregnation,

etc.).

Additional tests may be carried out using other species but, if so, this should be stated in the test

report.
7.2 Wood quality

The wood shall be free from visible cracks, stain, decay, insect damage and other defects. The

wood shall not have been water-stored, floated, chemically treated or steamed.
The wood should preferably be felled in winter.
Wood that has been kiln dried at temperatures below 60 °C may be used.
The wood should not have been stored for more than five years.

The wood shall be exclusively sapwood containing little resin and having between two and a half

annual rings per 10 mm and eight annual rings per 10 mm. The proportion of latewood in the

annual rings shall not exceed 30 % of the whole.

It is recommended to use test specimens of a similar growth rate within a single test.

7.3 Provision of test specimens

Prepare planed strips having a cross-section of (100 ± 2) mm × (25 ± 2) mm removing a minimum

of 2 mm from any surface exposed during drying. The longitudinal faces shall be parallel to the

direction of the grain. The annual rings shall be parallel to the broad faces (contact angle of less

than 35 °), (see Figure 1). Make transverse cuts neatly to give sharp edges and a fine-sawn finish

to the end-grain surfaces, to make test specimens (150 ± 2) mm long.

The test specimens shall originate from a minimum of three trees or shall be taken at random from

a stock originally of more than 100 test specimens.
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EN 1390:2020 (E)
7.4 Dimensions of test specimens

The test specimen at (12 ± 2) % a mass fraction of moisture content shall be (150 ± 2)

mm × (100 ± 2) mm × (25 ± 1) mm.

NOTE A moisture meter of the two-pronged electrical conductivity type is suitable for assessing

moisture content.
Mark each test specimen so that it can be identified throughout the test.
7.5 Number of test specimens

7.5.1 For fast and slow acting wood preservatives, test duration 12 weeks or 24 weeks

Use:

a) for each wood preservative and each concentration - 10 treated test specimens;

b) for a single test of each wood preservative - two untreated control test specimens.

7.5.2 For deferred acting wood preservatives, test duration 52 weeks
Use:

a) for each wood preservative and each concentration - 20 treated test specimens;

b) for a single test of each wood preservative -four untreated control test specimens.

8 Procedure
8.1 Preparation of the test specimens

Using the drill (5.3.4), drill vertically three holes, 30 mm deep, into each cross section of each test

specimen, positioning the holes as shown in Figure 1. For each hole choose the twist drill diameter

so as to provide a hole size which will accommodate the size of larva selected (8.2).

Place the test specimens in the testing chamber (5.3.3) for one week.
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SIST EN 1390:2020
EN 1390:2020 (E)
Dimensions in millimetres
a) frontal perspective view
b) horizontal section
Key
1 plan of section in Figure 1b)
2 insertion hole for larva
3 estimated test duration, in weeks
Figure 1 — Example of a test specimen
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SIST EN 1390:2020
EN 1390:2020 (E)
8.2 Insertion of larvae into the test specimens

For fast and slow acting wood preservatives, allocate three larvae from the 51 mg to 100 mg

weight range and three larvae from the 101 mg to 150 mg mass range to each test specimen.

Carefully insert the larvae (5.1) head first into the appropriately sized holes.

For deferred acting wood preservatives, allocate one larva from the 51 mg to 100 mg weight range

and two larvae from the 101 mg to 150 mg weight range to each test specimen (or vice versa).

Carefully insert the larvae (5.1) head first into the appropriately sized holes. Insert one larva from

one mass range into the middle hole at one end of the test specimen and two larvae from the other

mass range into outer holes of the other end of the test specimen.

Seal the insertion holes with plugs of cotton wool. Incubate the test specimens for one week in the

testing chamber (5.3.3), then remove the cotton wool plugs and determine whether each larva has

bored, replacing larvae, which have not bored. If any larvae are replaced then incubate all test

specimens for a further week in the testing chamber (5.3.3).
8.3 Sealing of the surfaces not to be treated
Seal the 100 mm × 150 mm pith face and the two cross sections.

a) For tests with preservative solutions in which water is the continuous phase, apply three

coats of paraffin wax (5.2.1) at about 90 °C so that the first coat adheres closely to the wood

and the successive coatings bond to one another;

b) for tests with preservative solutions in which the continuous phase is an organic solvent that

dissolves paraffin wax, use the gelatine (5.2.2). Apply the first coat with an aqueous solution

(200 g/l) at about 40 °C, then after a minimum of 8 h of drying, apply two further coats of an

aqueous solution (300 g/l) at about 50 °C;

c) other types of coating may be used if they are non-toxic to the test insects (5.1.1) and if their

performance as sealants has been documented.
8.4 Incubation of the test specimens

Incubate all the sealed test specimens in the testing chamber (5.3.3) for a maximum of two weeks

from the date at which larvae were first inserted.
8.5 Treatment of test specimens
8.5.1 Preparation of the treatment solution
8.5.1.1 Solid wood preservatives
Water-soluble wood preservatives:

— dissolve the wood preservative in the water (5.2.3) to the required working concentration.

Non-water-soluble wood preservatives:

— dissolve the wood preservative in an appropriate solvent (5.2.4) to the required working

concentration.
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EN 1390:2020 (E)
8.5.1.2 Liquid wood preservatives

If appropriate, use the wood preservative without further preparation other than any necessary

stirring. If it is a concentrate, dilute the wood preservative with the diluent to the required

working concentration, using the procedure specified by the manufacturer. For the dilution of

water-based wood preservatives use the water specified in 5.2.3.
All treatment solutions shall be freshly prepared.
8.5.2 Application of the treatment solution

Remove the test specimens to be treated from the testing chamber (5.3.3) after completion of the

incubation period (8.4). Determine the actual area of each unsealed surface to be treated taking

into account any possible encroachment of the sealing compound.
NOTE The total area to be treated is theoretically 225 cm .

Determine the volumes or masses of the treatment solution (8.5.1) to be applied to each unsealed

face to give the application rate specified by the supplier.

The quantity of the treatment solution to be applied should be realistic in view of the field of

application and the manufacturer's instructions. In the fume cupboard (5.3.2), using either the

pipette (5.3.5) or a brush (5.3.6), apply the appropriate calculated volume or mass of the

treatment solution (8.5.1) to each of the unsealed faces as uniformly as possible. Apply the

treatment solution to each unsealed face whilst keeping that face in a horizontal and upward

facing position. Allow any surface liquid to be absorbed into each face before treating the next

face.

If the required quantity cannot be applied in one application, the treatment solution may be

applied in successive applications at appropriately close intervals so as to avoid solidification of

any substances hindering the penetration of the subsequent applications.

If a brush application is used, weigh the test specimens during application to determine the mass

applied.

From the quantity of the treatment solution applied to each unsealed face of each treated test

specimen, determine and record the average application rate in g/m (brush application), or

ml/m (pipette application) of the treated test specimens.
Complete the treatment of all face
...

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