EN 17136:2019

Water quality - Guidance on field and laboratory procedures for quantitative analysis and identification of macroinvertebrates from inland surface waters

This document gives guidance on the quantitative estimation of abundance and identification of macroinvertebrates in samples taken from inland waters. The procedure deals with pre-treatment (cleaning), sub-sampling, sorting, and final identification of organisms from preserved and unpreserved samples originating from natural habitats or artificial substrates and their transport to the laboratory. Specific guidance is given for preservation for DNA-analysis.

Wasserbeschaffenheit - Anleitung zu Feld- und Laborverfahren zur quantitativen Analyse und Bestimmung von Makroinvertebraten aus Binnenoberflächengewässern

Dieses Dokument ist eine Anleitung zur quantitativen Beurteilung der Abundanz und Bestimmung von Makroinvertebraten in Proben aus Binnengewässern. Das Verfahren befasst sich mit der Vorbehandlung (Reinigung), Herstellen von Teilproben, Sortierung und endgültigen Bestimmung von Organismen aus konservierten und lebenden Proben, die aus natürlichen Lebensräumen oder künstlichen Substraten stammen, und ihrem Transport zum Labor. Für die DNA-Analyse wird eine spezifische Anleitung zur Konservierung gegeben.

Qualité de l'eau - Guide sur les procédures de terrain et de laboratoire pour l’analyse quantitative et l’identification des macro-invertébrés des eaux de surface continentales

Le présent document donne des conseils sur l’estimation quantitative de l’abondance et l’identification des macro-invertébrés dans des échantillons provenant de milieux aquatiques continentaux. La procédure s’applique au prétraitement (nettoyage), au sous-échantillonnage, au tri et à l’identification finale des organismes provenant d’échantillons avec ou sans conservateurs, issus d’habitats naturels ou d’échantillonneurs de colonisation, ainsi qu’à leur transport au laboratoire. Des conseils spécifiques sont donnés au sujet de la conservation en vue de l’analyse ADN.

Kakovost vode - Navodilo za terenske in laboratorijske postopke kvantitativne analize in identifikacije velikih nevretenčarjev v celinskih površinskih vodah

Ta standard podaja navodilo za oceno številčnosti in identifikacijo velikih nevretenčarjev v vzorcih iz celinskih voda. Postopek obravnava predobdelavo (čiščenje), podvzorčenje, razvrščanje in dokončno identifikacijo organizmov iz ohranjenih in živih vzorcev, ki izhajajo iz naravnih habitatov ali umetnih substratov.

General Information

• Status: Published
• Publication Date: 26-Feb-2019
• Withdrawal Date: 30-Aug-2019
• ICS: 13.060.10 - Water of natural resources
• Technical Committee: CEN/TC 230 - Water analysis
• Drafting Committee: CEN/TC 230/WG 21 - Invertebrates
• Current Stage: 9060 - Closure of 2 Year Review Enquiry - Review Enquiry
• Start Date: 02-Sep-2024
• Completion Date: 02-Sep-2024
• Ref Project: SIST EN 17136:2019 - Water quality - Guidance on field and laboratory procedures for quantitative analysis and identification of macroinvertebrates from inland surface waters

Standards Content (Sample)

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.YDQWLWDWLYQHWasserbeschaffenheit - Anleitung zu Feld- und Laborverfahren zur quantitativen Analyse und Identifizierung von Makroinvertebraten aus Inland-OberflächengewässernQualité de l'eau - Guide sur les modes opératoires de terrain et de laboratoire pour l'analyse quantitative et d'identification des macro-invertébrés des eaux de surface intérieuresWater quality - Guidance on field and laboratory procedures for quantitative analysis and identification of macroinvertebrates from inland surface waters13.060.70Preiskava bioloških lastnosti vodeExamination of biological properties of water13.060.10Voda iz naravnih virovWater of natural resourcesICS:Ta slovenski standard je istoveten z:EN 17136:2019SIST EN 17136:2019en,fr,de01-maj-2019SIST EN 17136:2019SLOVENSKI
STANDARD
EUROPEAN STANDARD NORME EUROPÉENNE EUROPÄISCHE NORM
EN 17136
February
t r s { ICS
s uä r x rä s r English Version
Water quality æ Guidance on field and laboratory procedures for quantitative analysis and identification of macroinvertebrates from inland surface waters Qualité de l 5eau æ Guide sur les procédures de terrain et de laboratoire pour l 5analyse quantitative et l 5identification des macroæinvertébrés des eaux de surface continentales
Wasserbeschaffenheit æ Anleitung zu Feldæ und Laborverfahren zur quantitativen Analyse und Bestimmung von Makroinvertebraten aus Binnenoberflächengewässern This European Standard was approved by CEN on
s v December
t r s zä
egulations which stipulate the conditions for giving this European Standard the status of a national standard without any alterationä Upætoædate lists and bibliographical references concerning such national standards may be obtained on application to the CENæCENELEC Management Centre or to any CEN memberä
translation under the responsibility of a CEN member into its own language and notified to the CENæCENELEC Management Centre has the same status as the official versionsä
CEN members are the national standards bodies of Austriaá Belgiumá Bulgariaá Croatiaá Cyprusá Czech Republicá Denmarká Estoniaá Finlandá Former Yugoslav Republic of Macedoniaá Franceá Germanyá Greeceá Hungaryá Icelandá Irelandá Italyá Latviaá Lithuaniaá Luxembourgá Maltaá Netherlandsá Norwayá Polandá Portugalá Romaniaá Serbiaá Slovakiaá Sloveniaá Spainá Swedená Switzerlandá Turkey and United Kingdomä
EUROPEAN COMMITTEE FOR STANDARDIZATION COMITÉ EUROPÉEN DE NORMALISATION EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre:
Rue de la Science 23,
B-1040 Brussels
t r s { CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Membersä Refä Noä EN
s y s u xã t r s { ESIST EN 17136:2019

Examples of sample splitters and sorting tray . 16 Bibliography . 18
3.1 fixation protection from disintegration of the morphological structure of organisms 3.2 macroinvertebrate invertebrate that is easily visible without magnification (> 0,5 mm) and generally belongs to the group of organisms that live for at least one life stage on or in the bottom substrate or vegetation of inland surface waters 3.3 matrix components of a sample other than the macroinvertebrates 3.4 preservation process that protects organic substances from decay 4 Principle The identification and quantification of macroinvertebrates comprises both field and laboratory procedures. After an optional pre-treatment of rinsing and sieving in the field, macroinvertebrate samples are transported to the laboratory where the samples are further processed. Macroinvertebrates are taken out of the matrix material by a sample dependent technique, quantified and sorted in functional groups for identification using a microscope or DNA-analysis. Several techniques exist to clean (remove unwanted matrix material) the sample and sort the organisms. The most suitable technique should be selected by visual inspection of the individual sample. Dependent on the objective of the analysis samples can be preserved in the field or cooled and processed unpreserved. SIST EN 17136:2019
...