Wood preservatives - Determination of the protective effectiveness against Lyctus brunneus (Stephens) - Part 2: Application by impregnation (Laboratory method)

This part of the EN 20 series specifies a method for the determination of the protective effectiveness or the toxic values of a wood preservative against infection by Lyctus brunneus (Stephens) in wood which has been treated previously by full impregantion.
This method is applicable to:
- water-insoluble chemicals which are being studied as active insecticides; or
- organic formulsation, as supplied or as prepared in the laboratory by dilution of concentrates.
This method is applicable to water-based preservatives.
NOTE This method can be used in conjuction with ageing procedures, which do not remove the added nutrient.

Holzschutzmittel - Bestimmung der vorbeugenden Wirkung gegenüber Lyctus brunneus (Stephens) - Teil 2: Anwendung durch Volltränkung (Laboratoriumsverfahren)

Dieser Teil der Normenreihe EN 20 legt ein Verfahren zur Bestimmung der vorbeugenden Wirkung oder der Grenze der Wirksamkeit eines Holzschutzmittels gegen Befall durch Lyctus brunneus (Stephens) fest, bei dem das Schutzmittel durch Volltränkung in das Holz eingebracht wird.
Das Verfahren ist anwendbar für
   nicht wasserlösliche chemische Erzeugnisse, die als insektizide Wirkstoffe untersucht werden, oder
   organische Formulierungen im Anlieferungszustand oder in der Form, wie sie im Labor durch Verdünnung von Konzentraten hergestellt werden.
Das Verfahren ist für wasserbasierte Schutzmittel anwendbar.
ANMERKUNG   Das Verfahren kann in Verbindung mit Alterungsverfahren angewendet werden, die die zugegebene Nährlösung nicht entfernen.

Produits de préservation du bois - Détermination de l'efficacité protectrice vis-à-vis de Lyctus brunneus (Stephens) - Partie 2 : Application par traitement en profondeur (Méthode de laboratoire)

La présente partie de la série de normes EN 20 spécifie une méthode de détermination de l'efficacité protectrice ou du seuil d'efficacité d'un produit de préservation du bois contre l'infestation par le Lyctus brunneus (Stephens) dans du bois préalablement traité par imprégnation totale.
La présente méthode est applicable :
- aux produits chimiques non hydrosolubles étudiés en tant que matières actives insecticides ; ou
- aux formules organiques telles qu'elles sont livrées ou obtenues en laboratoire par dilution de concentrés.
La présente méthode est applicable aux produits de préservation à base d'eau.
NOTE La présente méthode peut être utilisée conjointement avec des épreuves de vieillissement qui n'éliminent pas la solution nutritive ajoutée.

Zaščitna sredstva za les - Določanje učinkovitosti preventivne zaščite proti rjavem parketarju Lyctus brunneus (Stephens) - 2. del: Globinska impregnacija lesa (laboratorijska metoda)

Ta del skupine standardov EN 20 določa metodo za ugotavljanje učinkovitosti preventivne zaščite ali toksičnih vrednosti zaščitnega sredstva za les proti rjavemu parketarju Lyctus brunneus (Stephens), uporabljene na lesu, ki je bil obdelan s popolno impregnacijo.
Ta metoda se uporablja za:
– v vodi netopne kemikalije, ki se preučujejo kot aktivni insekticidi; ali
– organske formulacije, ki so dobavljene ali pripravljene v laboratoriju z redčenjem koncentratov.
Ta metoda se uporablja za zaščitna sredstva za les na vodni osnovi.
OPOMBA: Ta metoda se lahko uporablja v povezavi s postopki staranja, ki ne odstranjujejo dodanega hranila.

General Information

Status
Published
Publication Date
05-Dec-2023
Current Stage
6060 - Definitive text made available (DAV) - Publishing
Start Date
06-Dec-2023
Due Date
15-Feb-2024
Completion Date
06-Dec-2023

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SLOVENSKI STANDARD
01-marec-2024
Nadomešča:
SIST EN 20-2:1996
Zaščitna sredstva za les - Določanje učinkovitosti preventivne zaščite proti rjavem
parketarju Lyctus brunneus (Stephens) - 2. del: Globinska impregnacija lesa
(laboratorijska metoda)
Wood preservatives - Determination of the protective effectiveness against Lyctus
brunneus (Stephens) - Part 2: Application by impregnation (Laboratory method)
Holzschutzmittel - Bestimmung der vorbeugenden Wirkung gegenüber Lyctus brunneus
(Stephens) - Teil 2: Anwendung durch Volltränkung (Laboratoriumsverfahren)
Produits de préservation du bois - Détermination de l'efficacité protectrice vis-à-vis de
Lyctus brunneus (Stephens) - Partie 2 : Application par traitement en profondeur
(Méthode de laboratoire)
Ta slovenski standard je istoveten z: EN 20-2:2023
ICS:
71.100.50 Kemikalije za zaščito lesa Wood-protecting chemicals
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN 20-2
EUROPEAN STANDARD
NORME EUROPÉENNE
December 2023
EUROPÄISCHE NORM
ICS 71.100.50 Supersedes EN 20-2:1993
English Version
Wood preservatives - Determination of the protective
effectiveness against Lyctus brunneus (Stephens) - Part 2:
Application by impregnation (Laboratory method)
Produits de préservation du bois - Détermination de Holzschutzmittel - Bestimmung der vorbeugenden
l'efficacité protectrice vis-à-vis de Lyctus brunneus Wirkung gegenüber Lyctus brunneus (Stephens) - Teil
(Stephens) - Partie 2 : Application par traitement en 2: Anwendung durch Volltränkung
profondeur (Méthode de laboratoire) (Laboratoriumsverfahren)
This European Standard was approved by CEN on 6 November 2023.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATIO N

EUROPÄISCHES KOMITEE FÜR NORMUN G

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2023 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 20-2:2023 E
worldwide for CEN national Members.

Contents Page
European foreword . 4
Introduction . 5
1 Scope . 6
2 Normative references . 6
3 Terms and definitions . 6
4 Principle . 6
5 Test materials and apparatus . 7
5.1 Biological material . 7
5.2 Products and reagents . 7
5.3 Apparatus . 7
6 Sampling . 8
7 Test specimens . 8
7.1 Species of wood . 8
7.2 Quality of wood . 9
7.3 Provision of test specimens . 9
7.4 Dimensions of test specimens . 9
7.5 Number of test specimens . 9
8 Procedure. 9
8.1 Prior impregnation of the test specimens with a nutrient solution . 9
8.1.1 Composition of the nutrient solution . 9
8.1.2 Method of impregnation of nutrient solution . 10
8.1.3 Drying of test specimens . 10
8.2 Conditioning of specimens before end sealing . 10
8.3 Treatment of test specimens . 10
8.3.1 Preparation of treatment solutions . 10
8.3.2 Impregnation . 10
8.4 Drying and conditioning of the test specimens after treatment . 11
8.5 Exposure of the test specimens to the insects . 12
8.6 Conditions and duration of test . 12
8.7 Examination of the test specimens . 12
9 Validity of the test . 12
10 Expression of results . 13
10.1 Assessment of the protective effectiveness . 13
10.2 Toxic values . 13
11 Test report . 13
Annex A (informative) Example of a test report . 15
Annex B (informative) Technique for culturing Lyctus brunneus . 17
B.1 Introduction . 17
B.2 Diets . 17
B.2.1 Wood . 17
B.2.2 Artificial diet . 19
B.3 Obtaining adult beetles . 19
B.4 Culturing procedure . 20
B.5 Identification of sex . 20
B.6 Precautions against infestation by parasites . 20
Annex C (informative) Principal parasites and predators of Lyctus . 21
C.1 Mites . 21
C.2 Insects . 21
Bibliography . 22

European foreword
This document (EN 20-2:2023) has been prepared by Technical Committee CEN/TC 38 “Durability of
wood and wood-based products”, the secretariat of which is held by SIS.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by June 2024, and conflicting national standards shall be
withdrawn at the latest by June 2024.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN 20-2:1993.
The main changes compared to the previous edition EN 20-2:1993 are listed below:
a) the source of peptone is no longer specified (5.2.6);
b) other wood species than oak may be used for the test under certain circumstances (7.1);
c) tests with solvent control may be omitted, when the solvent is water (7.5);
d) test duration was alined with EN 20-1 and extended to 20 weeks (8.6);
e) new pictures were used for Figure B.1, Figure B.2 and Figure B.3.
NOTE Test results obtained according to earlier versions of this document and when the tests had started
before this version of EN 20-2 was published are considered valid.
Any feedback and questions on this document should be directed to the users’ national standards body.
A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organisations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia,
Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland,
Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of North
Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the United
Kingdom.
Introduction
This Part of the EN 20 series describes a laboratory method of testing which gives a basis for assessment
of the protective effectiveness of a wood preservative against Lyctus brunneus. It allows the
determination of the concentration at which the preservative completely prevents the development of
infestation from egg-laying in fully impregnated wood of susceptible species.
It can also be used with formulations ready for use.
The species Lyctus brunneus is chosen because of its particular practical relevance and because it can be
used easily in laboratory tests. The method can be used with other lyctid species, but the results might
not be comparable with those obtained with Lyctus brunneus.
The test specimens are enriched with a defined nutrient solution, before exposure to egg-laying, in order
to ensure uniformity of nutrient quality of test specimens between different laboratories.
This laboratory method provides one criterion by which the value of a product can be assessed. In making
this assessment, the methods by which the preservative may be applied should be taken into account. It
is further recommended that results from this test should be supplemented by those from other
appropriates tests, and above all by comparison with practical experience.
When products which are very active at low concentrations are used, it is very important to take suitable
precautions to isolate and separate, as far as possible, operations
...

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