EN 15607:2009
(Main)Foodstuffs - Determination of d-biotin by HPLC
Foodstuffs - Determination of d-biotin by HPLC
This European Standard specifies a method for the determination of the mass fraction of d-biotin by high performance liquid chromatography (HPLC). The method has been validated in an inter-laboratory test on fortified and non-fortifed samples such as cereal breakfast powder, infant milk powder, lyophilized green peas with ham, lyophilized chicken soup and on nutritive orange juice, at levels from 16 μg/100 g to 200 μg/100 g. For further information on the validation data, see Annex B. NOTE 1 d-biocytin can also be estimated by this method. But none of the samples used for the validation step contained d-biocytin. Nonetheless the recovery rate is more than 90 % for d-biotin and d-biocytin, see [2] and [3]. NOTE 2 The method underestimates the real biotin content when used for samples containing egg.
Lebensmittel - Bestimmung von D-Biotin mit HPLC
Diese Europäische Norm legt ein Verfahren zur Bestimmung des Massenanteils an D Biotin in Lebensmitteln mit Hochleistungsflüssigchromatographie (en: high performance liquid chromatography, HPLC) fest. Das Verfahren wurde in einem Ringversuch an angereicherten und naturbelassenen Proben wie pulverisierten Frühstückscerealien, Milchpulver für Säuglinge, grünen Erbsen mit Schinken (lyophilisiert), Hühnersuppe (lyophilisiert) und an angereichertem Orangensaft validiert, die 16 µg/100 g bis 200 µg/100 g an D Biotin enthielten. Weitere Informationen, siehe Anhang B.
ANMERKUNG 1 Mit diesem Verfahren kann auch D Biocytin bewertet werden, obwohl keine der Proben, die im Ringversuch verwendet wurden, D Biocytin enthielt. Dennoch beträgt die Wiederfindungsrate für D Biotin und D Biocytin mehr als 90 %, siehe [2] und [3].
ANMERKUNG 2 Bei der Untersuchung von Proben, die Ei enthalten, ergibt das Verfahren zu kleine Werte für den Biotin Gehalt.
Produits alimentaires - Dosage de la d-biotine par CLHP
La présente Norme européenne spécifie une méthode de détermination de la fraction massique de la d biotine par chromatographie liquide haute performance (CLHP). La méthode a été validée dans un essai interlaboratoire sur des échantillons enrichis et non enrichis tels que poudre de céréales pour petits déjeuners, lait en poudre pour nourrissons, petits pois au jambon lyophilisés, soupe au poulet lyophilisée et sur du jus d'orange nutritif, à des niveaux allant de 16 µg/100 g à 200 µg/100 g. Pour de plus amples informations sur les données de validation, voir l'Annexe B.
NOTE 1 La d-biocytine peut également être estimée par la présente méthode. Toutefois, aucun des échantillons utilisés dans l'étape de validation ne contenait de d-biocytine. Néanmoins, le taux de récupération est supérieur à 90 % pour la d-biotine et la d-biocytine (voir [2] et [3]).
NOTE 2 La méthode sous-estime la teneur réelle en biotine lorsqu'elle est utilisée avec des échantillons contenant de l'oeuf.
Živila - Določevanje d-biotina s HPLC
General Information
Standards Content (Sample)
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Lebensmittel - Bestimmung von d-Biotin mit HPLCProduits alimentaires - Dosage de la d-biotine par CLHPFoodstuffs - Determination of d-biotin by HPLC67.050Splošne preskusne in analizne metode za živilske proizvodeGeneral methods of tests and analysis for food productsICS:Ta slovenski standard je istoveten z:EN 15607:2009SIST EN 15607:2009en,fr,de01-september-2009SIST EN 15607:2009SLOVENSKI
STANDARD
SIST EN 15607:2009
EUROPEAN STANDARDNORME EUROPÉENNEEUROPÄISCHE NORMEN 15607May 2009ICS 67.050 English VersionFoodstuffs - Determination of d-biotin by HPLCProduits alimentaires - Dosage de la d-biotine par CLHPLebensmittel - Bestimmung von d-Biotin mit HPLCThis European Standard was approved by CEN on 23 April 2009.CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this EuropeanStandard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such nationalstandards may be obtained on application to the CEN Management Centre or to any CEN member.This European Standard exists in three official versions (English, French, German). A version in any other language made by translationunder the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as theofficial versions.CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland,France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.EUROPEAN COMMITTEE FOR STANDARDIZATIONCOMITÉ EUROPÉEN DE NORMALISATIONEUROPÄISCHES KOMITEE FÜR NORMUNGManagement Centre:
Avenue Marnix 17,
B-1000 Brussels© 2009 CENAll rights of exploitation in any form and by any means reservedworldwide for CEN national Members.Ref. No. EN 15607:2009: ESIST EN 15607:2009
EN 15607:2009 (E) 2 Contents Page Foreword .31Scope .42Normative references .43Principle .44Reagents .45Apparatus .76Procedure .87Calculation .98Precision .99Test report . 10Annex A (informative)
Typical chromatogram . 11Annex B (informative)
Precision data . 13Bibliography . 14 SIST EN 15607:2009
EN 15607:2009 (E) 3 Foreword This document (EN 15607:2009) has been prepared by Technical Committee CEN/TC 275 “Food analysis - Horizontal methods”, the secretariat of which is held by DIN. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by November 2009, and conflicting national standards shall be withdrawn at the latest by November 2009. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights. WARNING — The use of this standard may involve hazardous materials, operations and equipment. This standard does not purport to address all the
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