Animal feeding stuffs - Determination of nicarbazin - High-performance liquid chromatographic method

This European Standard specifies a method for the determination of additive use of nicarbazin in animal feeding stuffs and premixtures (maximum concentration 2,5% nicarbazin) using high performance liquid chromatography. Nicarbazin is a 1:1 equimolar mixture of 4,4’-dinitrocarbanilide (DNC) and 4,6-dimethyl-2-pyriminol (HDP). Nicarbazin is generally determined by using DNC as the target compound. In this method the DNC moiety of nicarbazin is detected.
The limit of quantitation is 20 mg/kg. The limit of detection is 0,5 mg/kg
NOTE   A lower limit of quantitation may be achievable but should be validated by the user.

Futtermittel - Bestimmung von Nicarbazin - Hochleistungsflüssigchromatographisches Verfahren

Diese Europäische Norm legt ein Verfahren zur Bestimmung von zugesetzten (eingemischten) Nicarbazin in
Futtermitteln und Vormischungen (maximale Konzentration 2,5 % Nicarbazin) mittels Hochleistungsflüssigchromatographie
fest. Nicarbazin ist ein 1 : 1 äquimolares Gemisch aus 4,4’-Dinitrocarbanilid (DNC) und
4,6-Dimethyl-2-pyriminol (HDP). Im Allgemeinen wird bei der Nicarbazin-Bestimmung DNC als Zielsubstanz
erfasst. In diesem Verfahren wird der DNC-Anteil von Nicarbazin bestimmt.
Die Bestimmungsgrenze beträgt 20 mg/kg. Die Nachweisgrenze beträgt 0,5 mg/kg.
ANMERKUNG Eine niedrigere Bestimmungsgrenze lässt sich erreichen, sie sollte jedoch durch den Anwender validiert
werden.

Aliments des animaux - Détermination de la nicarbazine - Méthode de chromatographie liquide haute performance

La présente Norme européenne spécifie une méthode permettant de déterminer l’utilisation d’un additif de
nicarbazine dans les aliments pour animaux et les pré-mélanges (concentration maximale en nicarbazine
2,5 %) à l’aide de la chromatographie liquide haute performance. La nicarbazine est un mélange
équimolaire 1:1 de 4,4’-dinitrocarbanilide (DNC) et de 4,6-diméthyl-2-pyrimidinol (HDP). La nicarbazine est
généralement déterminée par l’utilisation du DNC comme composé cible. La présente méthode permet de
détecter la fraction de DNC dans la nicarbazine.
La limite de quantification est de 20 mg/kg. La limite de détection est de 0,5 mg/kg.
NOTE Une limite de quantification plus faible est acceptable mais il convient que celle-ci soit validée par l’utilisateur.

Krma - Določevanje nikarbazina - Metoda tekočinske kromatografije visoke ločljivosti

General Information

Status
Published
Publication Date
04-Aug-2009
Current Stage
9060 - Closure of 2 Year Review Enquiry - Review Enquiry
Start Date
03-Mar-2024
Completion Date
03-Mar-2024

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Standards Content (Sample)

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.ORþOMLYRVWLFuttermittel - Bestimmung von Nicarbazin - Hochleistungsflüssigchromatographisches VerfahrenAliments des animaux - Détermination de la nicarbazine - Méthode de chromatographie liquide hautes performancesAnimal feeding stuffs - Determination of nicarbazin - High-performance liquid chromatographic method65.120KrmilaAnimal feeding stuffsICS:Ta slovenski standard je istoveten z:EN 15782:2009SIST EN 15782:2009en,fr,de01-december-2009SIST EN 15782:2009SLOVENSKI
STANDARD



SIST EN 15782:2009



EUROPEAN STANDARDNORME EUROPÉENNEEUROPÄISCHE NORMEN 15782August 2009ICS 65.120 English VersionAnimal feeding stuffs - Determination of nicarbazin - High-performance liquid chromatographic methodAliments des animaux - Détermination de la nicarbazine -Méthode de chromatographie liquide hautes performancesFuttermittel - Bestimmung von Nicarbazin -Hochleistungsflüssigchromatographisches VerfahrenThis European Standard was approved by CEN on 3 July 2009.CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this EuropeanStandard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such nationalstandards may be obtained on application to the CEN Management Centre or to any CEN member.This European Standard exists in three official versions (English, French, German). A version in any other language made by translationunder the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as theofficial versions.CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland,France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.EUROPEAN COMMITTEE FOR STANDARDIZATIONCOMITÉ EUROPÉEN DE NORMALISATIONEUROPÄISCHES KOMITEE FÜR NORMUNGManagement Centre:
Avenue Marnix 17,
B-1000 Brussels© 2009 CENAll rights of exploitation in any form and by any means reservedworldwide for CEN national Members.Ref. No. EN 15782:2009: ESIST EN 15782:2009



EN 15782:2009 (E) 2 Contents Page Foreword .3 1 Scope .4 2 Principle .4 3 Reagents .4 4 Apparatus .5 5 Sampling .5 6 Preparation of test sample .5 7 Procedure .6 8 Calculation and expression of results .7 9 Precision .8 10 Test report .8 Annex A (informative)
Results of an interlaboratory study .9 Bibliography . 10
SIST EN 15782:2009



EN 15782:2009 (E) 3 Foreword This document (EN 15782:2009) has been prepared by Technical Committee CEN/TC 327 “Animal feeding stuffs”, the secretariat of which is held by NEN. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by February 2010, and conflicting national standards shall be withdrawn at the latest by February 2010. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN [and/or CENE
...

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