Water quality - Evaluation of the "ready", "ultimate" aerobic biodegradability of organic compounds in an aqueous medium - Method by analysis of dissolved organic carbon (DOC) (ISO 7827:2010)

ISO 7827:2010 specifies a method for the evaluation of the “ready” and “ultimate” biodegradability of organic compounds at a given range of concentrations by aerobic microorganisms. In this context, ISO 7827:2010 also gives specific definitions for the terms “ready” and “ultimate”.
The method applies to organic compounds which are: a) soluble at the concentration used under the conditions of the test [dissolved organic carbon (DOC) concentrations of 10 mg/l to 40 mg/l]; b) non‑volatile or having a negligible vapour pressure under the conditions of the test; c) not significantly adsorbable on glass and activated sludge; d) not inhibitory to the test microorganisms at the concentration chosen for the test.
The method is not suitable for waste waters, as they usually contain significant amounts of water‑insoluble organic carbon, which is not included in DOC measurements.

Wasserbeschaffenheit - Bestimmung der leichten, vollständigen aeroben biologischen Abbaubarkeit organischer Stoffe in einem wässrigen Medium - Verfahren mittels Analyse des gelösten organischen Kohlenstoffs (DOC) (ISO 7827:2010)

Diese Internationale Norm legt ein Verfahren zur Bestimmung der "leichten" und "vollständigen" biologischen Abbaubarkeit organischer Substanzen durch aerobe Mikroorganismen bei vorgegebenen Konzentrationen fest. In diesem Zusammenhang definiert diese Internationale Norm auch die Begriffe "leicht" und "vollständig".
Das Verfahren ist für organische Substanzen anwendbar, die folgende Eigenschaften besitzen:
a)   löslich bei der verwendeten Konzentration unter den Testbedingungen (10 mg/l bis 40 mg/l DOC);
b)   nicht flüchtig oder mit einem vernachlässigbaren Dampfdruck unter den Testbedingungen;
c)   keine deutliche Adsorption an Glas und Belebtschlamm;
d)   nicht hemmend gegenüber den Mikroorganismen des Inokulums bei der gewählten Testkonzentration.
Das Verfahren ist nicht für Abwässer geeignet, da diese üblicherweise deutliche Mengen an wasserunlöslichem organischem Kohlenstoff enthalten, der nicht von DOC Messungen erfasst wird.

Qualité de l'eau - Évaluation de la biodégradabilité aérobie «facile», «ultime» des composés organiques en milieu aqueux - Méthode par analyse du carbone organique dissous (COD) (ISO 7827:2010)

L'ISO 7827:2010 spécifie une méthode d'évaluation de la biodégradabilité «facile» et «ultime» des composés organiques à une gamme de concentrations données sous l'action de micro-organismes aérobies. Dans ce contexte, l'ISO 7827:2010 fournit également des définitions spécifiques des termes «facile» et «ultime».
La méthode s'applique à des composés organiques qui sont: a) solubles à la concentration utilisée dans les conditions de l'essai [concentrations de 10 mg/l à 40 mg/I de carbone organique dissous (COD)]; b) non volatils, ou ayant une tension de vapeur négligeable dans les conditions de l'essai; c) non absorbables significativement sur le verre et les boues activées; d) non inhibiteurs, à la concentration choisie pour l'essai, vis-à-vis des micro-organismes d'essai.
La méthode ne convient pas aux eaux usées, car elles contiennent en général des quantités importantes de carbone organique insoluble dans l'eau, qui ne fait pas partie des mesurages COD.

Kakovost vode - Vrednotenje "lahke", "končne" aerobne biološke razgradljivosti organskih snovi v vodnem okolju - Metoda določevanja raztopljenega organskega ogljika (DOC) (ISO 7827:2010)

Ta mednarodni standard določa metodo za vrednotenje »lahke« in »končne« biološke razgradljivosti organskih snovi pri določenem razponu koncentracij aerobnih mikroorganizmov. V tem smislu standard ISO 7827:2010 tudi podaja posebne opredelitve za izraza »lahka« in »končna«. Metoda se uporablja za organske snovi z naslednjimi lastnostmi: a) so topne pri uporabljeni koncentraciji pod pogoji preskusa [koncentracije raztopljenega organskega ogljika (DOC) od 10 mg/l do 40 mg/l]; b) so nehlapne ali imajo pod pogoji preskusa zanemarljiv parni tlak; c) niso znatno adsorbljive na steklu in aktivnem blatu; d) pri koncentraciji, izbrani za preskus, niso zaviralne za preskusne mikroorganizme. Metoda ni primerna za odpadne vode, saj običajno vsebujejo velike količine v vodi netopnega organskega ogljika, ki ni zajet v meritve DOC.

General Information

Status
Withdrawn
Publication Date
30-Mar-2013
Withdrawal Date
29-Jun-2013
Technical Committee
Drafting Committee
Current Stage
6060 - Definitive text made available (DAV) - Publishing
Start Date
19-Dec-2012
Due Date
26-Feb-2013
Completion Date
19-Dec-2012

Relations

Buy Standard

Standard
EN ISO 7827:2013
English language
20 pages
sale 10% off
Preview
sale 10% off
Preview
e-Library read for
1 day

Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN ISO 7827:2013
01-januar-2013
1DGRPHãþD
SIST EN ISO 7827:1997
.DNRYRVWYRGH9UHGQRWHQMHODKNHNRQþQHDHUREQHELRORãNHUD]JUDGOMLYRVWL
RUJDQVNLKVQRYLYYRGQHPRNROMX0HWRGDGRORþHYDQMDUD]WRSOMHQHJDRUJDQVNHJD
RJOMLND '2&  ,62

Water quality - Evaluation of the "ready", "ultimate" aerobic biodegradability of organic

compounds in an aqueous medium - Method by analysis of dissolved organic carbon
(DOC) (ISO 7827:2010)

Wasserbeschaffenheit - Bestimmung der "leichten", "vollständigen" aeroben biologischen

Abbaubarkeit organischer Stoffe in einem wässrigen Medium - Verfahren mittels Analyse

des gelösten organischen Kohlenstoffs (DOC) (ISO 7827:2010)

Qualité de l'eau - Évaluation de la biodégradabilité aérobie «facile», «ultime» des

composés organiques en milieu aqueux - Méthode par analyse du carbone organique
dissous (COD) (ISO 7827:2010)
Ta slovenski standard je istoveten z: EN ISO 7827:2012
ICS:
13.060.70 Preiskava bioloških lastnosti Examination of biological
vode properties of water
SIST EN ISO 7827:2013 en,fr,de

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------
SIST EN ISO 7827:2013
---------------------- Page: 2 ----------------------
SIST EN ISO 7827:2013
EUROPEAN STANDARD
EN ISO 7827
NORME EUROPÉENNE
EUROPÄISCHE NORM
December 2012
ICS 13.060.70 Supersedes EN ISO 7827:1995
English Version
Water quality - Evaluation of the "ready", "ultimate" aerobic
biodegradability of organic compounds in an aqueous medium -
Method by analysis of dissolved organic carbon (DOC) (ISO
7827:2010)

Qualité de l'eau - Évaluation de la biodégradabilité aérobie Wasserbeschaffenheit - Bestimmung der leichten,

"facile", "ultime" des composés organiques en milieu vollständigen aeroben biologischen Abbaubarkeit

aqueux - Méthode par analyse du carbone organique organischer Stoffe in einem wässrigen Medium - Verfahren

dissous (COD) (ISO 7827:2010) mittels Analyse des gelösten organischen Kohlenstoffs

(DOC) (ISO 7827:2010)
This European Standard was approved by CEN on 8 December 2012.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European

Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national

standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by translation

under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same

status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,

Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,

Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United

Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: Avenue Marnix 17, B-1000 Brussels

© 2012 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 7827:2012: E

worldwide for CEN national Members.
---------------------- Page: 3 ----------------------
SIST EN ISO 7827:2013
EN ISO 7827:2012 (E)
Contents Page

Foreword ....................................................................................................................................................... 3

---------------------- Page: 4 ----------------------
SIST EN ISO 7827:2013
EN ISO 7827:2012 (E)
Foreword

The text of ISO 7827:2010 has been prepared by Technical Committee ISO/TC 147 “Water quality” of the

International Organization for Standardization (ISO) and has been taken over as EN ISO 7827:2012 by

Technical Committee CEN/TC 230 “Water analysis” the secretariat of which is held by DIN.

This European Standard shall be given the status of a national standard, either by publication of an identical

text or by endorsement, at the latest by June 2013, and conflicting national standards shall be withdrawn at

the latest by June 2013.

Attention is drawn to the possibility that some of the elements of this document may be the subject of patent

rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.

This document supersedes EN ISO 7827:1995.

According to the CEN/CENELEC Internal Regulations, the national standards organisations of the following

countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech

Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece,

Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,

Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom.

Endorsement notice

The text of ISO 7827:2010 has been approved by CEN as a EN ISO 7827:2012 without any modification.

---------------------- Page: 5 ----------------------
SIST EN ISO 7827:2013
---------------------- Page: 6 ----------------------
SIST EN ISO 7827:2013
INTERNATIONAL ISO
STANDARD 7827
Third edition
2010-11-15
Water quality — Evaluation of the
“ready”, “ultimate” aerobic
biodegradability of organic compounds
in an aqueous medium — Method by
analysis of dissolved organic carbon
(DOC)
Qualité de l'eau — Évaluation de la biodégradabilité aérobie «facile»,
«ultime» des composés organiques en milieu aqueux — Méthode par
analyse du carbone organique dissous (COD)
Reference number
ISO 7827:2010(E)
ISO 2010
---------------------- Page: 7 ----------------------
SIST EN ISO 7827:2013
ISO 7827:2010(E)
PDF disclaimer

This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but

shall not be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In

downloading this file, parties accept therein the responsibility of not infringing Adobe's licensing policy. The ISO Central Secretariat

accepts no liability in this area.
Adobe is a trademark of Adobe Systems Incorporated.

Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation

parameters were optimized for printing. Every care has been taken to ensure that the file is suitable for use by ISO member bodies. In

the unlikely event that a problem relating to it is found, please inform the Central Secretariat at the address given below.

COPYRIGHT PROTECTED DOCUMENT
© ISO 2010

All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means,

electronic or mechanical, including photocopying and microfilm, without permission in writing from either ISO at the address below or

ISO's member body in the country of the requester.
ISO copyright office
Case postale 56 • CH-1211 Geneva 20
Tel. + 41 22 749 01 11
Fax + 41 22 749 09 47
E-mail copyright@iso.org
Web www.iso.org
Published in Switzerland
ii © ISO 2010 – All rights reserved
---------------------- Page: 8 ----------------------
SIST EN ISO 7827:2013
ISO 7827:2010(E)
Contents Page

Foreword ............................................................................................................................................................iv

1 Scope......................................................................................................................................................1

2 Normative references............................................................................................................................1

3 Terms and definitions ...........................................................................................................................2

4 Principle .................................................................................................................................................3

5 Test environment...................................................................................................................................3

6 Reagents ................................................................................................................................................3

7 Apparatus...............................................................................................................................................4

8 Procedure...............................................................................................................................................5

9 Calculation and expression of results ................................................................................................7

10 Validity of the test..................................................................................................................................8

11 Test report..............................................................................................................................................9

Annex A (informative) Typical degradation curve .........................................................................................10

Annex B (informative) Interpretation of results .............................................................................................11

Bibliography......................................................................................................................................................13

© ISO 2010 – All rights reserved iii
---------------------- Page: 9 ----------------------
SIST EN ISO 7827:2013
ISO 7827:2010(E)
Foreword

ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies

(ISO member bodies). The work of preparing International Standards is normally carried out through ISO

technical committees. Each member body interested in a subject for which a technical committee has been

established has the right to be represented on that committee. International organizations, governmental and

non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the

International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.

International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.

The main task of technical committees is to prepare International Standards. Draft International Standards

adopted by the technical committees are circulated to the member bodies for voting. Publication as an

International Standard requires approval by at least 75 % of the member bodies casting a vote.

Attention is drawn to the possibility that some of the elements of this document may be the subject of patent

rights. ISO shall not be held responsible for identifying any or all such patent rights.

ISO 7827 was prepared by Technical Committee ISO/TC 147, Water quality, Subcommittee SC 5, Biological

methods.

This third edition cancels and replaces the second edition (ISO 7827:1994), which has been technically

revised.
iv © ISO 2010 – All rights reserved
---------------------- Page: 10 ----------------------
SIST EN ISO 7827:2013
INTERNATIONAL STANDARD ISO 7827:2010(E)
Water quality — Evaluation of the “ready”, “ultimate” aerobic
biodegradability of organic compounds in an aqueous
medium — Method by analysis of dissolved organic carbon
(DOC)

WARNING — Persons using this International Standard should be familiar with normal laboratory

practice. This standard does not purport to address all of the safety problems, if any, associated with

its use. It is the responsibility of the user to establish appropriate safety and health practices and to

ensure compliance with any national regulatory conditions.

IMPORTANT — It is absolutely essential that tests conducted according to this International Standard

be carried out by suitably trained staff.

SAFETY PRECAUTIONS — Activated sludge and sewage contain potentially pathogenic organisms.

Therefore take appropriate precautions when handling them. Handle toxic test compounds and those

whose properties are unknown with care.
1 Scope

This International Standard specifies a method for the evaluation of the “ready” and “ultimate” biodegradability

of organic compounds at a given range of concentrations by aerobic microorganisms. In this context, this

International Standard also gives specific definitions for the terms “ready” and “ultimate”.

The method applies to organic compounds which are:

a) soluble at the concentration used under the conditions of the test [dissolved organic carbon (DOC)

concentrations of 10 mg/l to 40 mg/l];

b) non-volatile or having a negligible vapour pressure under the conditions of the test;

c) not significantly adsorbable on glass and activated sludge;

d) not inhibitory to the test microorganisms at the concentration chosen for the test.

The method is not suitable for waste waters, as they usually contain significant amounts of water-insoluble

organic carbon, which is not included in DOC measurements.
2 Normative references

The following referenced documents are indispensable for the application of this document. For dated

references, only the edition cited applies. For undated references, the latest edition of the referenced

document (including any amendments) applies.

ISO 8245, Water quality — Guidelines for the determination of total organic carbon (TOC) and dissolved

organic carbon (DOC)

ISO 9408, Water quality — Evaluation of ultimate aerobic biodegradability of organic compounds in aqueous

medium by determination of oxygen demand in a closed respirometer
© ISO 2010 – All rights reserved 1
---------------------- Page: 11 ----------------------
SIST EN ISO 7827:2013
ISO 7827:2010(E)

ISO 9439, Water quality — Evaluation of ultimate aerobic biodegradability of organic compounds in aqueous

medium — Carbon dioxide evolution test
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1
degradation time

time from the end of the lag time, t , until the time that about 90 % of the maximum level of biodegradation has

been reached
NOTE Degradation time is expressed in days.
3.2
inherent biodegradation

level of biodegradation achieved which indicates the test compound is unlikely to be persistent in the

environment
NOTE See Annex B.
3.3
lag time
time from the start of the test until 10 % biodegradation has been reached
NOTE Lag time is expressed in days.
3.4
maximum level of biodegradation

degree of biodegradation of a chemical compound or organic matter in a test above which no further

biodegradation takes place during the test
3.5
primary biodegradation

structural change (transformation) of a chemical compound by microorganisms resulting in the loss of a

specific property of that compound
3.6
“ready” biodegradation

level of biodegradation achieved under defined conditions which indicates the test compound is considered

likely to degrade rapidly and completely under aerobic aquatic environmental conditions

NOTE See Annex B.
3.7
suspended solids

〈activated sludge〉 solid material within activated sludge with a particle diameter of >45 µm

NOTE The concentration of suspended solids is obtained by filtration or centrifugation of a known volume of sludge

under specified conditions, drying at 105 °C, and correcting for the volume of sample. The concentration of suspended

solids is expressed in milligrams per litre.
3.8
“ultimate” biodegradation

breakdown of a chemical compound or organic matter by microorganisms to carbon dioxide, water and

mineral salts of any other elements present (mineralization), and the production of new biomass

2 © ISO 2010 – All rights reserved
---------------------- Page: 12 ----------------------
SIST EN ISO 7827:2013
ISO 7827:2010(E)
4 Principle

The biodegradation of organic compounds by aerobic microorganisms in a mineral medium is determined by

measurement of the DOC concentration. The organic compound is the sole source of carbon in the medium.

The concentration of the compound used is such that the initial DOC in the medium is between 10 mg/l and

40 mg/l. If necessary, concentrations greater than 40 mg/l may be used. The test solution is aerated in the

dark or diffuse light at 22 °C ± 2 °C.

Biodegradation is monitored by measurement of the DOC at the start (day 0), at the end of the test (day 28 or

longer if necessary), and at a minimum of three intermediate time intervals. The percentage removal of DOC

is calculated at each time interval, and the biodegradability of the organic compound based on these data.

Specific analysis can give additional information on primary biodegradation.

The test is not suitable for compounds which are inhibitory at the concentration used in the test. Inhibitory

effects can be determined as specified in 8.3 or by using any other method for determining the inhibitory effect

[1]
on bacteria of a substance (e.g. ISO 8192 ).

The conditions specified in this International Standard do not necessarily correspond to the optimal conditions

allowing the maximum degree of biodegradation to occur. Tests for ready biodegradability have very stringent

conditions and a substance which passes these is considered likely to be rapidly and completely degraded in

any aerobic aquatic environmental compartment, especially in waste water treatment plants. For alternative

[6]
biodegradation methods, see ISO/TR 15462 .
See Annex B for information on interpretation of results.
5 Test environment

Incubation shall take place in the dark or in diffused light in an enclosure which is maintained at 22 °C ± 2 °C

and which is free from vapours that are toxic to microorganisms.
6 Reagents
Use only reagents of recognized analytical grade (where applicable).

6.1 Water, distilled or demineralized, containing less than 10 % of the initial DOC content introduced by the

compound to be tested to maintain acceptable precision.
6.2 Test medium
6.2.1 Composition
6.2.1.1 Solution A
Anhydrous potassium dihydrogenphosphate, KH PO 8,5 g
2 4
Anhydrous dipotassium hydrogenphosphate, K HPO 21,75 g
2 4
Disodium hydrogenphosphate dihydrate, Na HPO ·2HO 33,4 g
2 4 2
Ammonium chloride, NHCl 0,5 g
Water (6.1), quantity necessary to make up to 1 000 ml

Measure the pH value of the solution, which should be 7,4 ± 0,2. If it is not, then prepare a new solution.

© ISO 2010 – All rights reserved 3
---------------------- Page: 13 ----------------------
SIST EN ISO 7827:2013
ISO 7827:2010(E)
6.2.1.2 Solution B

Dissolve 22,5 g of magnesium sulfate heptahydrate (MgSO ·7H O) in water (6.1) and make up to 1 000 ml.

4 2
6.2.1.3 Solution C

Dissolve 27,5 g of anhydrous calcium chloride (CaCl ) in water (6.1) and make up to 1 000 ml.

6.2.1.4 Solution D

Dissolve 0,25 g of iron(III) chloride hexahydrate (FeCl ·6H O) in water (6.1) and make up to 1 000 ml. Prepare

3 2
the solution freshly just before use.

The need to prepare this solution just before use can be avoided if a drop of concentrated hydrochloric acid

(HCI) or 0,4 g/l of ethylenediaminetetraacetic acid (EDTA) is added.
6.2.2 Preparation
For 1 l of test medium, add to about 500 ml of water (6.1):
a) 1 ml of each of solutions B, C, and D;
b) 10 ml of solution A.

Make up to 1 000 ml with water (6.1). Solution A is added last, to avoid precipitation of salts. Prepare the test

medium freshly before use. Solutions A to C can be stored for up to 6 months in the dark at

...

Questions, Comments and Discussion

Ask us and Technical Secretary will try to provide an answer. You can facilitate discussion about the standard in here.