EN 16006:2011

Animal feeding stuffs - Determination of the Sum of Fumonisin B1 & B2 in compound animal feed with immunoaffinity clean-up and RP-HPLC with fluorescence detection after pre- or post-column derivatisation

This European Standard is applicable to the determination of Fumonisin B1 & B2 (FB1 & FB2) in compound animal feed at levels starting from 3 mg/kg up to 16 mg/kg.

Futtermittel - Bestimmung der Summe der Fumonisine B1 und B2 in Mischfutter durch Reinigung an einer Immunoaffinitätssäule und RP-HPLC mit Fluoreszenzdetektion nach Vor- oder Nachsäulenderivatisierung

Diese Europäische Norm ist für die Bestimmung des Gehaltes an Fumonisin B1 (FB1) und Fumonisin B2 (FB2) in Mischfuttermitteln bei Konzentrationen von 3 mg/kg bis zu 16 mg/kg anwendbar.

Aliments pour animaux - Dosage de la somme des fumonisines B1 et B2 dans les aliments pour animaux avec purification par immuno-affinité et RP-HPLC avec détection par fluorescence après dérivation pré- ou post-colonne

La présente Norme européenne est applicable au dosage des fumonisines B1 et B2 (FB1 et FB2) dans les
aliments pour animaux à des taux compris entre 3 mg/kg et 16 mg/kg.

Krma - Določanje vsote fumonizinov B1 in B2 v krmni mešanici z imunoafinitetnim čiščenjem in RP-HPLC s fluorescentno detekcijo po pred- ali pokolonski derivatizaciji

Ta evropski standard se uporablja za določanje fumonizinov B1 in B2 (FB1 & FB2) v krmni mešanici pri ravneh od 3 mg/kg do 16 mg/kg.

General Information

• Status: Published
• Publication Date: 02-Aug-2011
• ICS: 65.120 - Animal feeding stuffs
• Technical Committee: CEN/TC 327 - Animal feeding stuffs - Methods of sampling and analysis
• Drafting Committee: CEN/TC 327/WG 1 - Minerals and Contaminants
• Current Stage: 9060 - Closure of 2 Year Review Enquiry - Review Enquiry
• Start Date: 02-Sep-2022
• Completion Date: 02-Sep-2022
• Directive: 70/373/EC - Council Directive 70/373/EEC of 20 July 1970 on the introduction of Community methods of sampling and analysis for the official control of feeding-stuffs
• Mandate: M/382 - Mandate for standardisation addressed to CEN in the field of Methods of analysis for animal Feedingstuffs
• Ref Project: SIST EN 16006:2011 - Animal feeding stuffs - Determination of the Sum of Fumonisin B1 & B2 in compound animal feed with immunoaffinity clean-up and RP-HPLC with fluorescence detection after pre- or post-column derivatisation

Standards Content (Sample)

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.XQRDILQLWHWQLPRQVNLGHULYDWL]DFLMLFuttermittel - Bestimmung der Summe der Fumonisine B1 und B2 in Mischfutter mit Reinigung an einer Immunoaffinitätssäule und RP­HPLC­Verfahren mit Fluoreszenzdetektion nach Vor- oder NachsäulenderivatisierungAliments pour animaux - Dosage de la somme des fumonisines B1 et B2 dans les aliments pour animaux avec purification par immuno-affinité et RP-HPLC avec détection par fluorescence après dérivation pré- ou post-colonneAnimal feeding stuffs - Determination of the Sum of Fumonisin B1 & B2 in compound animal feed with immunoaffinity clean-up and RP-HPLC with fluorescence detection after pre- or post-column derivatisation65.120KrmilaAnimal feeding stuffsICS:Ta slovenski standard je istoveten z:EN 16006:2011SIST EN 16006:2011en,fr,de01-oktober-2011SIST EN 16006:2011SLOVENSKI
STANDARD



SIST EN 16006:2011



EUROPEAN STANDARD NORME EUROPÉENNE EUROPÄISCHE NORM
EN 16006
August 2011 ICS 65.120 English Version
Animal feeding stuffs - Determination of the Sum of Fumonisin B1 & B2 in compound animal feed with immunoaffinity clean-up and RP-HPLC with fluorescence detection after pre- or post-column derivatisation
Aliments pour animaux - Dosage de la somme des fumonisines B1 et B2 dans les aliments pour animaux avec purification par immuno-affinité et RP-HPLC avec détection par fluorescence après dérivation pré- ou post-colonne
Futtermittel - Bestimmung der Summe der Fumonisine B1 und B2 in Mischfutter durch Reinigung an einer Immunoaffinitätssäule und RP-HPLC mit Fluoreszenzdetektion nach Vor- oder Nachsäulenderivatisierung This European Standard was approved by CEN on 25 June 2011.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre:
Avenue Marnix 17,
B-1000 Brussels © 2011 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN 16006:2011: ESIST EN 16006:2011



EN 16006:2011 (E) 2 Contents Page Foreword .31Scope .42Normative references .43Principle .44Reagents and materials .45Apparatus .96Sample preparation . 107Measurements . 128Determination of concentrations . 159Precision . 1610Test report . 17Annex A (informative)
Precision data . 18Annex B (informative)
Examples of a chromatogram .
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