Soil quality - Biological methods - Chronic toxicity in higher plants (ISO 22030:2005)

ISO 22030:2005 describes a method for determining the inhibition of the growth and reproductive capability of higher plants by soils under controlled conditions. Two species are recommended: a rapid-cycling variant of turnip rape (Brassica rapa CrGC syn. Rbr) and oat (Avena sativa). The duration of test should be sufficient to include chronic endpoints that demonstrate the reproductive capability of the test plants.
By using natural test soils, e.g. from contaminated sites or remediated soils, and by comparing the development of the test plants in these soils with reference or standard control soils, the test is applicable to assess soil quality, especially the function of the soil as a habitat for plants.
This method can be modified to allow use of the chronic plant assay for the testing of chemicals incorporated into soil. By preparing a dilution series of a test substance in standard control soils, the same endpoints can be measured to assess the chronic toxicity of chemicals.
This method is not applicable to volatile substances, i.e. substances for which Henry's constant or the air/water partition coefficient is greater than 1, or for which the vapour pressure exceeds 0,013 3 Pa at 25 °C.

Bodenbeschaffenheit - Biologische Verfahren - Chronische Toxizität in höheren Pflanzen (ISO 22030:2005)

Diese Internationale Norm beschreibt ein Verfahren zur Bestimmung der Hemmwirkung von Böden auf das Wachstum und die Fortpflanzungsfähigkeit von höheren Pflanzen unter geregelten Bedingungen. Zwei Pflanzenarten werden empfohlen: eine schnell wachsende Sorte der Stoppelrübe (Brassica rapa CrGC oder Rbr) sowie Hafer (Saathafer) (Avena sativa). Die Prüfdauer sollte ausreichend bemessen sein, so dass sie chronische Endpunkte umfasst, durch die die Fortpflanzungsfähigkeit der Prüfpflanzen nachgewiesen werden kann.
Durch die Verwendung natürlicher Prüfböden, z. B. von verunreinigten Standorten oder sanierten Böden, und durch Vergleich der Entwicklung der Prüfpflanzen in diesen Böden mit deren Entwicklung in Referenz- oder Standard-Kontrollböden kann diese Prüfung dazu dienen, die Bodenqualität, insbesondere die Funktion des Bodens als Lebensraum für Pflanzen, zu bewerten.
In Anhang A sind die Modifikationen beschrieben, die vorzunehmen sind, um die chronische Pflanzenprüfung für die Prüfung von in den Boden eingemischten Chemikalien zu verwenden. Durch Herstellung einer Reihe von Verdünnungen einer Prüfsubstanz in Standard-Kontrollböden können dieselben Endpunkte zur Messung der chronischen Toxizität von Chemikalien verwendet werden. Dieses Verfahren ist nicht auf flüchtige Substanzen anwendbar, d. h. auf Substanzen, bei denen H (Henry-Konstante) oder der Luft-Wasser-Verteilungskoeffizient über 1 liegt oder bei denen der Dampfdruck bei 25 °C 0,013 3 Pa überschreitet.

Qualité du sol - Méthodes biologiques - Toxicité chronique sur les plantes supérieures (ISO 22030:2005)

L'ISO 22030:2005 décrit une méthode de détermination de l'inhibition de la croissance et de la capacité de reproduction des plantes supérieures par les sols dans des conditions contrôlées. Deux espèces sont recommandées: une variante de colza à cycle rapide (Brassica rapa CrGC syn. Rbr) et l'avoine (Avena sativa). Il convient que la durée de l'essai soit suffisante pour inclure les points de mesure chroniques qui apportent la preuve de la capacité de reproduction des plantes soumises à l'essai.
En utilisant des sols d'essai naturels provenant, par exemple, de sites contaminés ou de sols réhabilités, et en comparant le développement des plantes testées dans ces sols à celui des plantes testées dans les sols de référence ou les sols témoins standards, l'essai peut permettre d'évaluer la qualité du sol, notamment sa fonction d'habitat des plantes.
Cette méthode peut être modifiée pour permettre d'exploiter l'analyse chronique des plantes pour les essais de substances ou de produits chimiques incorporés dans le sol. En préparant une série de dilutions d'une substance dans les sols témoins standard, il est possible de déterminer les mêmes points de mesure afin d'évaluer la toxicité chronique de ces substances ou de ces produits chimiques.
Cette méthode n'est pas applicable aux substances volatiles, c'est-à-dire celles pour lesquelles la constante de Henry ou le coefficient de partage air/eau est supérieur à 1, ou pour lesquelles la pression de vapeur à 25 °C est supérieure à 0,013 3 Pa à 25 °C.

Kakovost tal - Biološke metode - Kronična strupenost za višje rastline (ISO 22030:2005)

ISO 22030:2005 opisuje metodo za določanje zaviranja rasti in razmnoževalne zmožnosti višjih rastlin na tleh v nadzorovanih pogojih. Priporočata se dve vrsti: varianta oljne repice s hitrim ciklom (Brassica rapa CrGC syn. Rbr) in oves (Avena sativa). Preskus naj traja dovolj dolgo, da vključi kronične končne točke, ki dokazujejo razmnoževalno zmožnost preskusnih rastlin.
Z uporabo naravnih preskusnih tal, npr. iz onesnaženih lokacij ali saniranih tal, in s primerjavo razvoja preskusnih rastlin na teh tleh in na referenčnih oziroma standardnih kontrolnih tleh se preskus lahko uporabi za ocenjevanje kakovosti tal, predvsem funkcije tal kot habitata za rastline.
Ta metoda se lahko modificira, da omogoči uporabo kroničnega poskusa na rastlinah za preskušanje kemikalij, vključenih v tla. S pripravo serije razredčitev preskusne snovi v standardnih kontrolnih tleh se lahko merijo iste končne točke za oceno kronične toksičnosti kemikalij.
Ta metoda ne velja za hlapne snovi, tj. snovi, pri katerih je Henryjeva konstanta ali porazdelitveni koeficient zrak/voda večji od 1 ali pri katerih parni tlak presega 0.013 3 Pa pri 25 °C.

General Information

Status
Published
Publication Date
28-Jun-2011
Withdrawal Date
30-Dec-2011
Current Stage
6060 - Definitive text made available (DAV) - Publishing
Start Date
29-Jun-2011
Due Date
03-Jun-2012
Completion Date
29-Jun-2011

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2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Bodenbeschaffenheit - Biologische Verfahren - Chronische Toxizität in höheren Pflanzen (ISO 22030:2005)Qualité du sol - Méthodes biologiques - Toxicité chronique sur les plantes supérieures (ISO 22030:2005)Soil quality - Biological methods - Chronic toxicity in higher plants (ISO 22030:2005)13.080.30Biološke lastnosti talBiological properties of soilsICS:Ta slovenski standard je istoveten z:EN ISO 22030:2011SIST EN ISO 22030:2011en,fr,de01-november-2011SIST EN ISO 22030:2011SLOVENSKI
STANDARD
EUROPEAN STANDARD NORME EUROPÉENNE EUROPÄISCHE NORM
EN ISO 22030
June 2011 ICS 13.080.30 English Version
Soil quality - Biological methods - Chronic toxicity in higher plants (ISO 22030:2005)
Qualité du sol - Méthodes biologiques - Toxicité chronique sur les plantes supérieures (ISO 22030:2005)
Bodenbeschaffenheit - Biologische Verfahren -Chronische Toxizität in höheren Pflanzen (ISO 22030:2005) This European Standard was approved by CEN on 10 June 2011.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre:
Avenue Marnix 17,
B-1000 Brussels © 2011 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN ISO 22030:2011: ESIST EN ISO 22030:2011

Reference numberISO 22030:2005(E)© ISO 2005
INTERNATIONAL STANDARD ISO22030First edition2005-02-01Soil quality — Biological methods — Chronic toxicity in higher plants Qualité du sol — Méthodes biologiques — Toxicité chronique sur les plantes supérieures
ISO 22030:2005(E) PDF disclaimer This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but shall not be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In downloading this file, parties accept therein the responsibility of not infringing Adobe's licensing policy. The ISO Central Secretariat accepts no liability in this area. Adobe is a trademark of Adobe Systems Incorporated. Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation parameters were optimized for printing. Every care has been taken to ensure that the file is suitable for use by ISO member bodies. In the unlikely event that a problem relating to it is found, please inform the Central Secretariat at the address given below.
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ISO 2005 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from either ISO at the address below or ISO's member body in the country of the requester. ISO copyright office Case postale 56 • CH-1211 Geneva 20 Tel.
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ii
ISO 22030:2005(E) © ISO 2005 – All rights reserved
iii Contents Page Foreword.iv Introduction.v 1 Scope.1 2 Normative references.1 3 Terms and definitions.2 4 Principle.4 5 Materials.4 5.1 Test plants.4 5.2 Soil and soil storage.4 5.2.1 General.4 5.2.2 Test soil.5 5.2.3 Control soil.5 5.3 Reference substance.6 6 Apparatus.6 7 Methods.8 7.1 Experimental design.8 7.2 Pre-test.8 7.3 Preliminary test (range-finding).8 7.4 Final test.9 7.5 Preparation of the pots.9 7.6 Preparation of the seeds.9 7.7 Growth conditions.9 7.8 Start of the test.10 7.9 Handling during the test.10 7.9.1 Number of plants and thinning out.10 7.9.2 Watering.10 7.9.3 Rearrangement of test vessels.10 7.9.4 Pollination.10 7.9.5 Reporting.11 7.10 Endpoint measurements.11 7.10.1 Seedling emergence.11 7.10.2 Harvest at day 14.11 7.10.3 Final harvest.11 7.11 Summary and timetable of the test.12 8 Validity criteria.13 9 Assessment of the results.13 9.1 Presentation of measured data.13 9.2 Statistical analysis.14 9.2.1 Preliminary test.14 9.2.2 Final test.14 9.2.3 NOEC (no observed effect concentration) approach.14 9.2.4 ECx (effect concentration) approach.14 10 Test report.15 Annex A (informative)
Testing of chemicals in soil.16 Bibliography.17
ISO 22030:2005(E) iv
ISO 22030:2005(E) © ISO 2005 – All rights reserved
v Introduction This International Standard describes a procedure for evaluating the quality of soils of different origin carrying unknown contaminations. The method, slightly modified, can also be used to measure the toxicity of known chemicals incorporated into soil.
The evaluation of the inhibition and chronic toxicity is based on emergence, vegetative growth and reproductive capacity of at least two species of higher plants.
This International Standard is based on: a) results of the research project “Development of a chronic bioassay using higher plants”, sponsored by the German Ministry for Education and Research (BMBF), Bonn [3], and b) discussions within t
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