EN ISO 5815-1:2019

Water quality - Determination of biochemical oxygen demand after n days (BODn) - Part 1: Dilution and seeding method with allylthiourea addition (ISO 5815-1:2019)

This document specifies the determination of the biochemical oxygen demand of waters by dilution and seeding with suppression of nitrification after 5 d or 7 d incubation time.
It is applicable to all waters having biochemical oxygen demands usually between 1 mg/l and 6 000 mg/l. It applies particularly to waste waters but also suits for the analysis of natural waters. For biochemical oxygen demands greater than 6 000 mg/l of oxygen, the method is still applicable, but special care is needed taking into consideration the representativeness of subsampling for preparation of the dilution steps. The results obtained are the product of a combination of biochemical and chemical reactions in presence of living matter which behaves only with occasional reproducibility. The results do not have the rigorous and unambiguous character of those resulting from, for example, a single, well‑defined, chemical process. Nevertheless, the results provide an indication from which the quality of waters can be estimated.

Wasserbeschaffenheit - Bestimmung des biochemischen Sauerstoffbedarfs nach n Tagen (BSBn) - Teil 1: Verdünnungs- und Impfverfahren mit Zugabe von Allylthioharnstoff (ISO 5815-1:2019)

Dieses Dokument legt die Bestimmung des biochemischen Sauerstoffbedarfs in Wässern durch Verdünnung und Impfen mit Nitrifikationshemmung nach 5 d oder 7 d Inkubationsdauer fest.
Es ist für alle Wässer geeignet, deren biochemischer Sauerstoffbedarf in der Regel zwischen 1 mg/l und 6 000 mg/l liegt. Es ist insbesondere für Abwässer anwendbar, ist aber auch für natürliche Wässer geeignet. Für einen biochemischen Sauerstoffbedarf von mehr als 6 000 mg/l Sauerstoff ist das Verfahren zwar noch anwendbar, aber besondere Vorsicht im Hinblick auf die Repräsentativität der Teilproben zur Herstellung der Verdünnungen ist geboten. Die erzielten Ergebnisse beruhen auf einer Kombination von biochemischen und chemischen Reaktionen unter Beteiligung von lebender Materie, welche sich nur selten reproduzierbar verhält. Die erhaltenen Ergebnisse haben daher keinen exakten und eindeutigen Charakter wie z. B. die eines einzelnen gut definierten chemischen Prozesses, liefern aber dennoch einen Hinweis, der auf die Beschaffenheit von Wässern schließen lässt.

Qualité de l'eau - Détermination de la demande biochimique en oxygène après n jours (DBOn) - Partie 1: Méthode par dilution et ensemencement avec apport d'allylthiourée (ISO 5815-1:2019)

Le présent document spécifie la méthode de détermination de la demande biochimique en oxygène dans les eaux, par dilution et ensemencement, avec suppression de la nitrification après une durée d'incubation de 5 jours ou 7 jours.
Cette méthode est applicable à tous les types d'eau dont la demande biochimique en oxygène se situe généralement entre 1 mg/l et 6 000 mg/l. Elle s'applique particulièrement aux eaux usées mais convient également à l'analyse des eaux naturelles. Pour des demandes biochimiques en oxygène de plus de 6 000 mg/l d'oxygène, la méthode est encore applicable, mais une attention particulière est nécessaire concernant la représentativité du sous-échantillonnage pour la préparation des étapes de dilution. Les résultats obtenus sont le produit d'une combinaison de réactions biochimiques et chimiques en présence de matière vivante dont le comportement et les caractéristiques ne sont qu'occasionnellement reproductibles. Les résultats ne possèdent pas le caractère exact et rigoureux de résultats issus par exemple d'un processus chimique unique bien défini. Les résultats fournissent cependant une indication qui permet d'estimer la qualité des eaux.

Kakovost vode - Določevanje biokemijske potrebe po kisiku po n dneh (BPKn) - 1. del: Metoda razredčevanja in cepljenja z dodatkom aliltiosečnine (ISO 5815-1:2019)

Ta del standarda ISO 5815 opredeljuje določevanje biokemijske potrebe po kisiku pri razredčevanju in cepljenju z zaviranjem nitrifikacije po 5-dnevni inkubacijski dobi.
Ta del standarda ISO 5815 se uporablja za vse vode z biokemijsko potrebo po kisiku, ki je običajno med 3 mg/l in 6000 mg/l. Uporablja se predvsem za odpadne vode. Če je biokemijska potreba po kisiku večja od 6000 mg/l kisika, se metoda še vedno uporablja, vendar lahko napake zaradi potrebnega razredčevanja vplivajo na kakovost analize pri tej preskusni metodi. Rezultate je treba razlagati previdno.
Pridobljeni rezultati so posledica kombinacije biokemijskih in kemijskih reakcij, v katerih sodelujejo žive snovi in se odzivajo le z občasno ponovljivostjo. Nimajo strogega in nedvoumnega značaja kot na primer tiste, ki nastanejo z enim natančno določenim kemijskim procesom. Kljub temu služijo kot kazalnik, na podlagi katerega je mogoče oceniti kakovost vode.

General Information

• Status: Published
• Publication Date: 17-Sep-2019
• Withdrawal Date: 30-Mar-2020
• ICS: 13.060.50 - Examination of water for chemical substances
• Technical Committee: CEN/TC 230 - Water analysis
• Drafting Committee: CEN/TC 230 - Water analysis
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 18-Sep-2019
• Completion Date: 18-Sep-2019
• Ref Project: SIST EN ISO 5815-1:2019 - Water quality - Determination of biochemical oxygen demand after n days (BODn) - Part 1: Dilution and seeding method with allylthiourea addition (ISO 5815-1:2019)

Relations

• Replaces: EN 1899-1:1998 - Water quality - Determination of biochemical oxygen demand after n days (BODn) - Part 1: Dilution and seeding method with allylthiourea addition (ISO 5815:1989, modified)
• Effective Date: 30-Oct-2019
• Replaces: EN 1899-2:1998 - Water quality - Determination of biochemical oxygen demand after n days (BODn) - Part 2: Method for undiluted samples (ISO 5815:1989, modified)
• Effective Date: 25-Sep-2019

Standards Content (Sample)

SLOVENSKI STANDARD
01-november-2019
Nadomešča:
SIST EN 1899-1:2000
SIST EN 1899-2:2000
Kakovost vode - Določevanje biokemijske potrebe po kisiku po n dneh (BPKn) - 1.
del: Metoda razredčevanja in cepljenja z dodatkom aliltiosečnine (ISO 5815-1:2019)
Water quality - Determination of biochemical oxygen demand after n days (BODn) - Part
1: Dilution and seeding method with allylthiourea addition (ISO 5815-1:2019)
Wasserbeschaffenheit - Bestimmung des biochemischen Sauerstoffbedarfs nach n
Tagen (BSBn) - Teil 1: Verdünnungs- und Impfverfahren mit Zugabe von Allylthioharnstoff
(ISO 5815-1:2019)
Qualité de l'eau - Détermination de la demande biochimique en oxygène après n jours
(DBOn) - Partie 1: Méthode par dilution et ensemencement avec apport d'allylthiourée
(ISO 5815-1:2019)
Ta slovenski standard je istoveten z: EN ISO 5815-1:2019
ICS:
13.060.50 Preiskava vode na kemične Examination of water for
snovi chemical substances
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 5815-1
EUROPEAN STANDARD
NORME EUROPÉENNE
September 2019
EUROPÄISCHE NORM
ICS 13.060.50 Supersedes EN 1899-2:1998
English Version
Water quality - Determination of biochemical oxygen
demand after n days (BODn) - Part 1: Dilution and seeding
method with allylthiourea addition (ISO 5815-1:2019)
Qualité de l'eau - Détermination de la demande Wasserbeschaffenheit - Bestimmung des
biochimique en oxygène après n jours (DBOn) - Partie biochemischen Sauerstoffbedarfs nach n Tagen (BSBn)
1: Méthode par dilution et ensemencement avec apport - Teil 1: Verdünnungs- und Impfverfahren mit Zugabe
d'allylthiourée (ISO 5815-1:2019) von Allylthioharnstoff (ISO 5815-1:2019)
This European Standard was approved by CEN on 26 July 2019.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2019 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 5815-1:2019 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
This document (EN ISO 5815-1:2019) has been prepared by Technical Committee ISO/TC 147 "Water
quality" in collaboration with Technical Committee CEN/TC 230 “Water analysis” the secretariat of
which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by March 2020, and conflicting national standards shall
be withdrawn at the latest by March 2020.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN 1899-2:1998.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the
United Kingdom.
Endorsement notice
The text of ISO 5815-1:2019 has been approved by CEN as EN ISO 5815-1:2019 without any
modification.
INTERNATIONAL ISO
STANDARD 5815-1
Second edition
2019-07
Water quality — Determination of
biochemical oxygen demand after n
days (BOD ) —
n
Part 1:
Dilution and seeding method with
allylthiourea addition
Qualité de l'eau — Détermination de la demande biochimique en
oxygène après n jours (DBO ) —
n
Partie 1: Méthode par dilution et ensemencement avec apport
d'allylthiourée
Reference number
ISO 5815-1:2019(E)
©
ISO 2019
ISO 5815-1:2019(E)
© ISO 2019
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting
on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address
below or ISO’s member body in the country of the requester.
ISO copyright office
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Published in Switzerland
ii © ISO 2019 – All rights reserved

ISO 5815-1:2019(E)
Contents Page
Foreword .iv
Introduction .vi
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 2
4 Principle . 3
5 Reagents . 3
6 Apparatus . 6
7 Sampling and preservation . 6
8 Interferences . 7
8.1 General . 7
8.2 Presence of free and/or combined chlorine . 7
8.3 Presence of algae . 7
8.4 Presence of peroxides and peroxide compounds. 8
9 Procedure. 8
9.1 General . 8
9.2 Pretreatment . 8
9.2.1 Neutralization of the sample . 8
9.2.2 Homogenization . 9
9.3 Preparation of test solutions . 9
9.4 Calculation of dilutions . 9
9.4.1 Empirical determination of the dilutions . 9
9.4.2 Determination of dilutions via the factors R of the TOC, the permanganate
index or the COD .10
9.4.3 Calculation of dilution stages via the COD .11
9.5 Blank value determination .11
9.6 Determination of dissolved oxygen .11
9.6.1 Measurement of dissolved oxygen using iodometric method (in
accordance with ISO 5813) .11
9.6.2 Measurement of dissolved oxygen using probes (in accordance with
ISO 5814 or ISO 17289) .12
9.7 Control analysis .12
10 Calculation and indication of the results .13
10.1 Examination of test solutions for valid oxygen consumption during test .13
10.2 Calculation of biochemical oxygen demand after n days (BOD ) .13
n
10.3 Validity criteria .14
11 Test report .14
Annex A (normative) Influence of incubation periods and temperatures .15
Annex B (informative) Multitesting .16
Annex C (informative) Direct seeding of the analysis batches .19
Annex D (informative) Performance data .20
Bibliography .22
ISO 5815-1:2019(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on
...