EN ISO 6865:2000
(Main)Animal feeding stuffs - Determination of crude fibre content - Method with intermediate filtration (ISO 6865:2000)
Animal feeding stuffs - Determination of crude fibre content - Method with intermediate filtration (ISO 6865:2000)
Cette norme internationale prescrit une méthode de détermination de la teneur en cellulose brute par filtration intermédiaire. Elle décrit un mode opératoire manuel et un mode opératoire semi-automatique. Cette méthode est applicable aux aliments des animaux ayant une teneur en cellulose brute supérieure à 10 g/kg.
NOTE Pour des aliments pour animaux contenant de la cellulose brute inférieure ou égale à 10 g/kg, la méthode décrite dans l'ISO 6541 doit être utilisée. Cette Norme internationale est également applicable aux céréales et aux légumes.»
Futtermittel - Bestimmung des Rohfasergehaltes - Verfahren mit Zwischenfiltration (ISO/FDIS 6865:2000)
Diese Internationale Norm legt ein Verfahren zur Bestimmung des Rohfasergehaltes mit Zwischenfiltration fest. Es wird sowohl ein manuelles Verfahren als auch ein halbautomatisches Verfahren beschrieben. Dieses Verfahren ist auf Futtermittel mit einem Rohfasergehalt von größer als 10 g/kg anwendbar. ANMERKUNG Für Futtermittel mit einem Rohfasergehalt kleiner als oder gleich 10 g/kg darf das in ISO 6541 : 1981 beschriebene Verfahren angewandt werden. Diese Internationale Norm ist auch auf Getreide und Hülsenfrüchte anwendbar.
Aliments des animaux - Détermination de la teneur en cellulose brute - Méthode avec filtration intermédiaire (ISO/FDIS 6865:2000)
Cette norme internationale prescrit une méthode de détermination de la teneur en cellulose brute par filtration intermédiaire. Elle décrit un mode opératoire manuel et un mode opératoire semi-automatique. Cette méthode est applicable aux aliments des animaux ayant une teneur en cellulose brute supérieure à 10 g/kg.
NOTE Pour des aliments pour animaux contenant de la cellulose brute inférieure ou égale à 10 g/kg, la méthode décrite dans l'ISO 6541 doit être utilisée. Cette Norme internationale est également applicable aux céréales et aux légumes.»
Krmila - Določevanje surovih vlaken - Metoda z vmesno filtracijo (ISO 6865:2000)
General Information
Standards Content (Sample)
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Futtermittel - Bestimmung des Rohfasergehaltes - Verfahren mit Zwischenfiltration (ISO/FDIS 6865:2000)Aliments des animaux - Détermination de la teneur en cellulose brute - Méthode avec filtration intermédiaire (ISO/FDIS 6865:2000)Animal feeding stuffs - Determination of crude fibre content - Method with intermediate filtration (ISO 6865:2000)65.120KrmilaAnimal feeding stuffsICS:Ta slovenski standard je istoveten z:EN ISO 6865:2000SIST EN ISO 6865:2001en01-februar-2001SIST EN ISO 6865:2001SLOVENSKI
STANDARD
ReferencenumberISO6865:2000(E)©ISO2000INTERNATIONALSTANDARDISO6865Firstedition2000-10-15Animalfeedingstuffs—Determinationofcrudefibrecontent—MethodwithintermediatefiltrationAlimentsdesanimaux—Déterminationdelateneurencellulosebrute—MéthodeavecfiltrationintermédiaireSIST EN ISO 6865:2001
ISO6865:2000(E)PDFdisclaimerThisPDFfilemaycontainembeddedtypefaces.InaccordancewithAdobe'slicensingpolicy,thisfilemaybeprintedorviewedbutshallnotbeeditedunlessthetypefaceswhichareembeddedarelicensedtoandinstalledonthecomputerperformingtheediting.Indownloadingthisfile,partiesacceptthereintheresponsibilityofnotinfringingAdobe'slicensingpolicy.TheISOCentralSecretariatacceptsnoliabilityinthisarea.AdobeisatrademarkofAdobeSystemsIncorporated.DetailsofthesoftwareproductsusedtocreatethisPDFfilecanbefoundintheGeneralInforelativetothefile;thePDF-creationparameterswereoptimizedforprinting.EverycarehasbeentakentoensurethatthefileissuitableforusebyISOmemberbodies.Intheunlikelyeventthataproblemrelatingtoitisfound,pleaseinformtheCentralSecretariatattheaddressgivenbelow.©ISO2000Allrightsreserved.Unlessotherwisespecified,nopartofthispublicationmaybereproducedorutilizedinanyformorbyanymeans,electronicormechanical,includingphotocopyingandmicrofilm,withoutpermissioninwritingfromeitherISOattheaddressbeloworISO'smemberbodyinthecountryoftherequester.ISOcopyrightofficeCasepostale56CH-1211Geneva20Tel.+41227490111Fax+41227490947E-mailcopyright@iso.chWebwww.iso.chPrintedinSwitzerlandii©ISO2000–AllrightsreservedSIST EN ISO 6865:2001
ISO6865:2000(E)©ISO2000–AllrightsreservediiiForewordISO(theInternationalOrganizationforStandardization)isaworldwidefederationofnationalstandardsbodies(ISOmemberbodies).TheworkofpreparingInternationalStandardsisnormallycarriedoutthroughISOtechnicalcommittees.Eachmemberbodyinterestedinasubjectforwhichatechnicalcommitteehasbeenestablishedhastherighttoberepresentedonthatcommittee.Internationalorganizations,governmentalandnon-governmental,inliaisonwithISO,alsotakepartinthework.ISOcollaboratescloselywiththeInternationalElectrotechnicalCommission(IEC)onallmattersofelectrotechnicalstandardization.InternationalStandardsaredraftedinaccordancewiththerulesgivenintheISO/IECDirectives,Part3.DraftInternationalStandardsadoptedbythetechnicalcommitteesarecirculatedtothememberbodiesforvoting.PublicationasanInternationalStandardrequiresapprovalbyatleast75%ofthememberbodiescastingavote.AttentionisdrawntothepossibilitythatsomeoftheelementsofthisInternationalStandardmaybethesubjectofpatentrights.ISOshallnotbeheldresponsibleforidentifyinganyorallsuchpatentrights.InternationalStandardISO6865waspreparedbyTechnicalCommitteeISO/TC34,Foodproducts,SubcommitteeSC10,Animalfeedingstuffs.AnnexAofthisInternationalStandardisforinformationonly.SIST EN ISO 6865:2001
INTERNATIONALSTANDARDISO6865:2000(E)©ISO2000–Allrightsreserved1Animalfeedingstuffs—Determinationofcrudefibrecontent—Methodwithintermediatefiltration1ScopeThisInternationalStandardspecifiesamethodwithintermediatefiltrationforthedeterminationofthecrudefibrecontent.Amanualprocedureandasemi-automaticprocedurearedescribed.Themethodisapplicabletoanimalfeedingstuffswithacrudefibrecontentgreaterthan10g/kg.NOTEForanimalfeedingstuffswithacrudefibrecontentequaltoorlessthan10g/kg,themethoddescribedinISO6541[7]maybeused.ThisInternationalStandardisalsoapplicabletocerealsandpulses.2NormativereferencesThefollowingnormativedocumentscontainprovisionswhich,throughreferenceinthistext,constituteprovisionsofthisInternationalStandard.Fordatedreferences,subsequentamendmentsto,orrevisionsof,anyofthesepublicationsdonotapply.However,partiestoagreementsbasedonthisInternationalStandardareencouragedtoinvestigatethepossibilityofapplyingthemostrecenteditionsofthenormativedocumentsindicatedbelow.Forundatedreferences,thelatesteditionofthenormativedocumentsreferredtoapplies.MembersofISOandIECmaintainregistersofcurrentlyvalidInternationalStandards.ISO3696:1987,Waterforanalyticallaboratoryuse—Specificationandtestmethods.ISO6498:1998,Animalfeedingstuffs—Preparationoftestsamples.3TermanddefinitionForthepurposesofthisInternationalStandard,thefollowingtermanddefinitionapply.3.1crudefibrecontentlossinmassresultingfromashingofthedriedresidueobtainedafteracidandalkalinedigestionofthesamplebytheproceduredescribedinthisInternationalStandard,dividedbythemassofthetestportionNOTEThecrudefibrecontentisexpressedingramsperkilogram.Itmayalsobeexpressedasamassfractioninpercent.4PrincipleThetestportionistreatedwithboilingdilutesulfuricacid.Theresidueisseparatedbyfiltration,washedandthentreatedwithboilingpotassiumhydroxidesolution.Theresidueisseparatedbyfiltration,washed,dried,weighedandthenashed.Thelossinmassresultingfromashingcorrespondstothemassofcrudefibreinthetestportion.SIST EN ISO 6865:2001
ISO6865:2000(E)2©ISO2000–Allrightsreserved5ReagentsandmaterialsUseonlyreagentsofrecognizedanalyticalgrade.5.1Water,complyingwithatleastgrade3inaccordancewithISO3696.5.2Hydrochloricacid,c(HCl)=0,5mol/l.5.3Sulfuricacid,c(H2SO4)=(0,130,005)mol/l.5.4Potassiumhydroxidesolution,c(KOH)=(0,230,005)mol/l.5.5Acetone.5.6Filteraid:seasand,orCelite®1)545,ormaterialofanequivalentquality.Beforeuse,treatseasandwithboilinghydrochloricacid[c(HCl)=4mol/l],washwithwateruntilfreefromacidandheatatatemperatureof(50025)°Cforatleast1h.Beforeuse,heatotherfilteraidsatatemperatureof(50025)°Cforatleast4h.5.7Antifoamagent,forinstancen-octanol.5.8Lightpetroleum,boilingrange40°Cto60°C.6ApparatusUsuallaboratoryapparatusand,inparticular,thefollowing.6.1Grindingdevice,capableofgrindingthesamplesothatitpassescompletelythroughasievewith1mmapertures.6.2Analyticalbalance,withaccuracyofatleast0,1mg.6.3Filtercrucibles,ofquartz,porcelainorhardglass,withfusedsinteredfilterplatewithaporesizeof40mto100m(porositygradeP100accordingtoISO4793:1980[1]).Beforeusingforthefirsttime,carefullyandgraduallyheatanewfiltercrucibletoatemperaturenotexceeding525°Candleaveforafewminutesat(50025)°C.Stainless-steelcrucibleswithstainless-steelsieveplateswithanaperturesizeof90mofidenticalperformancecharacteristicsmaybeusedasanalternative.6.4Porcelainsieveplates.6.5Incinerationdishes.6.6Beakersorconicalflasks,of500mlcapacity,providedwithasuitablecoolingdevice,forexampleacondensororadish.1)Celite®isthetradenameofacommerciallyavailableproduct.ThisinformationisgivenfortheconvenienceofusersofthisInternationalStandardanddoesnotconstituteanendorsementbyISOofthisproduct.Equivalentproductsmaybeusediftheycanbeshowntoleadtothesameresults.SIST EN ISO 6865:2001
ISO6865:2000(E)©ISO2000–Allrightsreserved36.7Dryingoven,electricallyheatedandventilated,capableofbeingmaintainedatatemperatureof(1302)°C.6.8Desiccator,containingbluesilicagelasdesiccant,providedwithaperforatedplate,preferablyofaluminiumorstainlesssteel,ofthickness2mmto3mm.6.9Mufflefurnace,electricallyheated,providedwithaircirculationandtemperaturecontrolcapableofmaintainingthetemperaturearoundthecruciblestothenearest25°Cattemperaturesof475°Cto525°C.Thereadingofthepyrometerofthemufflefurnacecannotalwaysbetrusted:deviationsmayoccur.Therefore,thetemperatureinthemufflefurnaceshouldbecheckedregularly.Dependingonthesizeandtypeofthemufflefurnace,temperaturesinthefurnacemaydifferfromoneplacetoanotherplace.Whentheovendoorisclosed,adequateairsupplyshouldbeguaranteed.Thevolumeflowrateofairshouldnotbesogreatthatmaterialiscarriedawayfromthecrucibles.6.10Cold-extractiondevice,providedwithasupportforthefiltercrucible(6.3);adischargepipewithataptothevacuumandliquidoutlet;andconnectingringsforconnectingthefiltercrucible(6.3).6.11Heatingdevice(formanualmethod),providedwithasuitablecoolingdevicecapableofmaintainingthevolumeconstantduringboiling.6.12Heatingdevice(forthesemi-automaticmethod)foracidandalkalinedigestion,providedwithasupportforthefiltercrucible(6.3);adischargepipewithataptothevacuumandliquidoutlet;aboilingcylinderofatleast270mlcapacity,witharefluxcondenser;connectingringsforconnectingtheheatingdevicetothefiltercrucible(6.3)andtheboilingcylinder;andoptionally,provisionforcompressedair.Beforeuse,preheattheapparatuswithboilingwaterfor5min.7SamplingSamplingisnotpartofthemethodspecifiedinthisInternationalStandard.ArecommendedsamplingmethodisgiveninISO6497[6].Itisimportantthatthelaboratoryreceiveasamplewhichistrulyrepresentativeandhasnotbeendamagedorchangedduringtransportorstorage.8PreparationoftestsamplePreparethetestsampleinaccordancewithISO6498.Usingthegrindingdevice(6.1),grindtheair-drylaboratorysamplesothatitpassescompletelythroughasievewith1mmapertures.Mixthoroughly.SIST EN ISO 6865:2001
ISO6865:2000(E)4©ISO2000–AllrightsreservedForthemanualmethod,proceedinaccordancewithclause9.Forthesemi-automaticmethod,proceedinaccordancewithclause10.9Procedureformanualmethod9.1TestportionWeighabout1gofthepreparedtestsample(clause8)tothenearest0,1mg(m1).Ifthefatcontentofthesampleexceeds100g/kg,orifthesamplecontainsfatswhichcannotbeextracteddirectlywithlightpetroleum(5.8),transferthesampletoacrucible(6.3)andproceedinaccordancewith9.2.Ifthefatcontentofthesampledoesnotexceed100g/kg,transferthesampletoabeaker(6.6)andproceedinaccordancewith9.3ifthecarbonatecontent,expressedascalciumcarbonate,exceeds50g/kg.Ifthisisnotthecase,proceedinaccordancewith9.4.9.2PreliminarydefattingInthecold-extractiondevice(6.10),defatthesamplethreetimesundervacuumwith30mloflightpetroleum(5.8)eachtime.Drytheresiduebysuctionaftereachwashing.Transfertheresiduetoabeaker(6.6).Ifthecarbonatecontent,expressedascalciumcarbonate,exceeds50g/kg,proceedinaccordancewith9.3.Ifnot,proceedinaccordancewith9.4.9.3RemovalofcarbonatePour100mlofhydrochloricacid(5.2)overthesample.Stircontinuouslyfor5min.Carefullypourthemixtureintoafiltercrucible,thebottomofwhichiscoveredwithathinlayeroffilteraid(5.6).Decanttwicewith100mlofwatereachtime.Takecaresothataslittlematerialaspossibleendsuponthefilter.Transferthecontentsofthecrucibletotheoriginalbeakerandproceedinaccordancewith9.4.9.4AciddigestionPour150mlofsulfuricacid(5.3)overthesample.Bringtotheboilasquicklyaspossibleandcontinuesteadyboilingfor(301)min.Atthebeginningofboiling,swirlafewtimes.Iffoamingoccurs,addafewdropsofantifoamagent(5.7).Duringboiling,maintainaconstantvolumebyusingasuitablecoolingdevice(see6.6and6.11).9.5FirstfiltrationInthefiltercrucible(6.3)applyalayeroffilteraid
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