EN ISO 22718:2015/A1:2022
(Amendment)Cosmetics - Microbiology - Detection of Staphylococcus aureus - Amendment 1 (ISO 22718:2015/Amd 1:2022)
Cosmetics - Microbiology - Detection of Staphylococcus aureus - Amendment 1 (ISO 22718:2015/Amd 1:2022)
Kosmetische Mittel - Mikrobiologie - Nachweis von Staphylococcus aureus - Änderung 1 (ISO 22718:2015/Amd 1:2022)
No scope available
Cosmétiques - Microbiologie - Détection de Staphylococcus aureus - Amendement 1 (ISO 22718:2015/Amd 1:2022)
Kozmetika - Mikrobiologija - Ugotavljanje prisotnosti bakterije Staphylococcus aureus - Dopolnilo A1 (ISO 22718:2015/Amd 1:2022)
General Information
Relations
Overview
EN ISO 22718:2015/A1:2022 is Amendment 1 to the international standard for Cosmetics - Microbiology - Detection of Staphylococcus aureus. Approved by CEN on 10 September 2022 and prepared by ISO/TC 217 in collaboration with CEN/TC 392, this amendment is endorsed without modification and is intended for national adoption by March 2023. The document updates enrichment media options used during microbiological testing of cosmetic products to support detection of Staphylococcus aureus.
Key Topics
- Addition of Modified Eugon LT broth: The amendment explicitly allows Modified Eugon LT broth as an alternative enrichment medium to Eugon LT 100 liquid broth.
- Composition: The amendment provides the composition of Modified Eugon LT broth as follows:
- Pancreatic digest of casein: 15 g
- Papaic digest of soybean meal: 5 g
- Sodium chloride: 4 g
- L-cystine: 0.7 g
- Sodium sulphite: 0.2 g
- Glucose: 5.5 g
- Egg lecithin: 1 g
- Polysorbate 80: 15 g
- Sodium lauryl ether sulfate: 1.56 g
- Water: 1000 ml
- Preparation and sterilization: Instructions specify dissolving polysorbate 80 and egg lecithin into boiling water first, then dissolving remaining components while heating; dispense into containers and sterilize at 121 °C for 15 minutes. Mix while still hot to redissolve settled substances. Final pH after sterilization should be 7.0 ± 0.2 at room temperature.
- Document structure change: The former subclause for other enrichment broths is renumbered (former 5.3.3.2 to 5.3.3.3) to accommodate the new subclause for Modified Eugon LT.
Applications
This amendment is directly relevant to laboratories, manufacturers and quality control professionals involved in cosmetic microbiology testing. Practical applications include:
- Implementing the Modified Eugon LT broth as an alternative enrichment medium in S. aureus detection workflows.
- Preparing validated culture media in-house according to the specified composition and sterilization parameters.
- Ensuring compliance with updated European adoption and national standardization requirements for cosmetics microbiology testing.
Benefits:
- Provides a documented, standardized alternative enrichment formulation for S. aureus detection.
- Facilitates consistent media preparation and reproducible test conditions across laboratories.
Related Standards
- ISO 22718:2015 - base international standard for detection of Staphylococcus aureus in cosmetics (this amendment modifies that text).
- CEN/TC 392 and ISO/TC 217 publications on cosmetics microbiology and related testing methods.
For implementation questions or national guidance, refer to your national standards body or the CEN-CENELEC Management Centre. This amendment exists in English, French and German official versions and may be translated by CEN members into other national languages.
Standards Content (Sample)
SLOVENSKI STANDARD
01-april-2023
Kozmetika - Mikrobiologija - Ugotavljanje prisotnosti bakterije Staphylococcus
aureus - Dopolnilo A1 (ISO 22718:2015/Amd 1:2022)
Cosmetics - Microbiology - Detection of Staphylococcus aureus - Amendment 1 (ISO
22718:2015/Amd 1:2022)
Kosmetische Mittel - Mikrobiologie - Nachweis von Staphylococcus aureus - Änderung 1
(ISO 22718:2015/Amd 1:2022)
Cosmétiques - Microbiologie - Détection de Staphylococcus aureus - Amendement 1
(ISO 22718:2015/Amd 1:2022)
Ta slovenski standard je istoveten z: EN ISO 22718:2015/A1:2022
ICS:
07.100.40 Kozmetika - mikrobiologija Cosmetics microbiology
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EN ISO 22718:2015/A1
EUROPEAN STANDARD
NORME EUROPÉENNE
September 2022
EUROPÄISCHE NORM
ICS 07.100.40
English Version
Cosmetics - Microbiology - Detection of Staphylococcus
aureus - Amendment 1 (ISO 22718:2015/Amd 1:2022)
Cosmétiques - Microbiologie - Détection de Kosmetische Mittel - Mikrobiologie - Nachweis von
Staphylococcus aureus - Amendement 1 (ISO Staphylococcus aureus - Änderung 1 (ISO
22718:2015/Amd 1:2022) 22718:2015/Amd 1:2022)
This amendment A1 modifies the European Standard EN ISO 22718:2015; it was approved by CEN on 10 September 2022.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for inclusion of
this amendment into the relevant national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This amendment exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the
same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2022 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 22718:2015/A1:2022 E
worldwide for CEN national Members.
Contents Page
European foreword . 3
European foreword
This document (EN ISO 22718:2015/A1:2022) has been prepared by Technical Committee ISO/TC 217
"Cosmetics" in collaboration with Technical Committee CEN/TC 392 “Cosmetics” the secretariat of
which is held by AFNOR.
This Amendment to the European Standard EN ISO 22718:2015 shall be given the status of a national
standard, either by publication of an identical text or by endorsement, at the latest by March 2023, and
conflicting national standards shall be withdrawn at the latest by March 2023.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
Any feedback and questions on this document should be directed to the users’ national standards
body/national committee. A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the
United Kingdom.
Endorsement notice
The text of ISO 22718:2015/Amd 1:2022 has been approved by CEN as EN ISO 22718:2015/A1:2022
without any modification.
...
Frequently Asked Questions
EN ISO 22718:2015/A1:2022 is a amendment published by the European Committee for Standardization (CEN). Its full title is "Cosmetics - Microbiology - Detection of Staphylococcus aureus - Amendment 1 (ISO 22718:2015/Amd 1:2022)". This standard covers: No scope available
No scope available
EN ISO 22718:2015/A1:2022 is classified under the following ICS (International Classification for Standards) categories: 07.100.40 - Cosmetics microbiology. The ICS classification helps identify the subject area and facilitates finding related standards.
EN ISO 22718:2015/A1:2022 has the following relationships with other standards: It is inter standard links to EN ISO 22718:2015. Understanding these relationships helps ensure you are using the most current and applicable version of the standard.
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