EN 14133:2009

Foodstuffs - Determination of ochratoxin A in wine and beer - HPLC method with immunoaffinity column clean-up

This European Standard specifies a method for the determination of ochratoxin A content in wine and beer using immunoaffinity column clean up and high performance liquid chromatography (HPLC), see [2] and [3]. This method has been validated in an interlaboratory study according to AOAC International Guidelines [4] for collaborative study procedures to validate characteristics of a method of analysis for the determination of ochratoxin A in wine and beer via the analysis of naturally contaminated and spiked samples of wine and beer at
levels ranging from 0,1 ng/ml to 3 ng/ml.

Lebensmittel - Bestimmung von Ochratoxin A in Wein und Bier - HPLC-Verfahren mit Reinigung an einer Immunoaffinitätssäule

Diese Europäische Norm legt ein hochleistungs flüssigchromatographisches Verfahren (HPLC) mit Reinigung an einer Immunoaffinitätssäule (IAS) zur Bestimmung von Ochratoxin A in Wein und Bier fest, siehe [2], [3].
Dieses Verfahren wurde in einem Ringversuch nach den AOAC "Guidelines for collaborative study procedures to validate characteristics of a method of analysis" [4] mit natürlich kontaminierten und aufgestockten Bier- und Weinproben mit Ochratoxin A Gehalten von 0,1 ng/ml bis 3 ng/ml validiert.

Produits alimentaires - Dosage de l'ochratoxine A dans le vin et la bière - Méthode par purification sur colonne d'immuno-affinité suivie d'une analyse par chromatographie liquide haute performance (CLHP)

La présente Norme européenne spécifie une méthode de dosage de l’ochratoxine A dans le vin et dans la
bière par chromatographie liquide à haute performance (CLHP) avec purification sur colonne
d’immuno-affinité, voir [2] et [3].
Cette méthode a été validée dans le cadre d’une étude interlaboratoires réalisée conformément aux directives
internationales de l’AOAC [4] relatives aux modes opératoires d’études comparatives visant à valider les
caractéristiques d’une méthode d’analyse, pour le dosage de l’ochratoxine A dans le vin et la bière, via
l’analyse d’échantillons naturellement contaminés et dopés, dans les limites d’une gamme de concentrations
comprise entre 0,1 ng/ml et 3 ng/ml.

Živila - Določevanje ohratoksina A v vinu in pivu - Metoda HPLC z imunoafinitetnim kolonskim čiščenjem

General Information

• Status: Published
• Publication Date: 26-May-2009
• Withdrawal Date: 29-Nov-2009
• ICS: 67.160.10 - Alcoholic beverages
• Technical Committee: CEN/TC 275 - Food analysis - Horizontal methods
• Drafting Committee: CEN/TC 275/WG 5 - Biotoxins
• Current Stage: 9093 - Decision to confirm - Review Enquiry
• Completion Date: 28-Oct-2020
• Ref Project: SIST EN 14133:2009 - Foodstuffs - Determination of ochratoxin A in wine and beer - HPLC method with immunoaffinity column clean-up

Relations

• Replaces: EN 14133:2003 - Foodstuffs - Determination of ochratoxin A in wine and beer - HPLC method with immunoaffinity column clean-up
• Effective Date: 08-Jun-2022
• Replaces: EN 14133:2003/AC:2006 - Foodstuffs - Determination of ochratoxin A in wine and beer - HPLC method with immunoaffinity column clean-up
• Effective Date: 30-May-2009

Standards Content (Sample)

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Lebensmittel - Bestimmung von Ochratoxin A in Wein und Bier - HPLC-Verfahren mit Reinigung an einer ImmunoaffinitätssäuleProduits alimentaires - Dosage de l'ochratoxine A dans le vin et la bière - Méthode par purification sur colonne d'immuno-affinité suivie d'une analyse par chromatographie liquide haute performance (CLHP)Foodstuffs - Determination of ochratoxin A in wine and beer - HPLC method with immunoaffinity column clean-up67.160.10Alcoholic beveragesICS:Ta slovenski standard je istoveten z:EN 14133:2009SIST EN 14133:2009en,fr,de01-december-2009SIST EN 14133:2009SLOVENSKI
STANDARDSIST EN 14133:2003/AC:2007SIST EN 14133:20031DGRPHãþD



SIST EN 14133:2009



EUROPEAN STANDARDNORME EUROPÉENNEEUROPÄISCHE NORMEN 14133May 2009ICS 67.160.10Supersedes EN 14133:2003
English VersionFoodstuffs - Determination of ochratoxin A in wine and beer -HPLC method with immunoaffinity column clean-upProduits alimentaires - Dosage de l'ochratoxine A dans levin et la bière - Méthode par purification sur colonned'immuno-affinité suivie d'une analyse par chromatographieliquide haute performance (CLHP)Lebensmittel - Bestimmung von Ochratoxin A in Wein undBier - HPLC-Verfahren mit Reinigung an einerImmunoaffinitätssäuleThis European Standard was approved by CEN on 24 May 2009.CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this EuropeanStandard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such nationalstandards may be obtained on application to the CEN Management Centre or to any CEN member.This European Standard exists in three official versions (English, French, German). A version in any other language made by translationunder the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as theofficial versions.CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland,France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.EUROPEAN COMMITTEE FOR STANDARDIZATIONCOMITÉ EUROPÉEN DE NORMALISATIONEUROPÄISCHES KOMITEE FÜR NORMUNGManagement Centre:
Avenue Marnix 17,
B-1000 Brussels© 2009 CENAll rights of exploitation in any form and by any means reservedworldwide for CEN national Members.Ref. No. EN 14133:2009: ESIST EN 14133:2009



EN 14133:2009 (E) 2 Contents Foreword . 3 1 Scope . 4 2 Normative references . 4 3 Principle . 4 4 Reagents . 4 5 Apparatus . 6 6 Procedure . 7 7 HPLC analysis . 7 8 Calculation . 8 9 Precision . 8 10 Test report . 10 Annex A (informative)
Precision data . 11 Bibliography . 13
SIST EN 14133:2009



EN 14133:2009 (E) 3 Foreword This document (EN 14133:2009) has been prepared by Technical Committee CEN
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