EN ISO 22065:2020

Workplace air - Gases and vapours - Requirements for evaluation of measuring procedures using pumped samplers (ISO 22065:2020)

This document specifies performance requirements and test methods under prescribed laboratory conditions for the evaluation of pumped samplers used in conjunction with an air sampling pump and of procedures using these samplers for the determination of gases and vapours in workplace atmospheres.
This document addresses requirements for method developers and/or manufacturers.
NOTE 1 For the purposes of this document, a manufacturer can be any commercial or non-commercial entity.
NOTE 2 For the sampling of semi-volatile compounds which can appear as a mixture of vapours and airborne particles in workplace atmospheres see EN 13936.
This document is applicable to pumped samplers and measuring procedures using these samplers in which sampling and analysis are carried out in separate stages.
This document is not applicable to:
—     pumped samplers which are used for the direct determination of concentrations, for example, length-of-stain detector tubes;
—     samplers which rely on sorption into a liquid, and subsequent analysis of the solution (bubblers).

Luft am Arbeitsplatz - Gase und Dämpfe - Anforderungen an die Evaluierung von Messverfahren mit pumpenbetriebenen Probenahmeeinrichtungen (ISO 22065:2020)

Dieses Dokument legt Anforderungen an die Leistungsfähigkeit und Prüfverfahren für die Evaluierung von pumpenbetriebenen Sammlern unter vorgegebenen Laborbedingungen fest, die in Verbindung mit einer Luftsammelpumpe verwendet werden, sowie von Verfahren, die diese Sammler zur Bestimmung von Gasen und Dämpfen in der Arbeitsplatzatmosphäre verwenden.
Dieses Dokument behandelt Anforderungen für Entwickler des Verfahrens und/oder Hersteller.
ANMERKUNG 1 Für den Zweck dieses Dokuments kann ein Hersteller jede kommerzielle oder nicht-kommerzielle Entität sein.
ANMERKUNG 2 Für die Probenahme von schwerflüchtigen Verbindungen, die als Mischung von Dampf und luftgetragenen Partikeln in Arbeitsplatzatmosphären auftreten können, siehe EN 13936.
Dieses Dokument gilt für pumpenbetriebene Sammler und diese Sammler verwendende Messverfahren, bei denen Probenahme und Analyse in getrennten Schritten vorgenommen werden.
Dieses Dokument gilt nicht für
- pumpenbetriebene Sammler, die zur direkten Konzentrationsbestimmung verwendet werden, z. B. Prüfröhrchen mit Längenanzeige;
- Sammler, deren Funktionsweise auf Sorption in einer Flüssigkeit und anschließender Analyse der Lösung beruht (Gaswaschflaschen).

Air des lieux de travail - Gaz et vapeurs - Exigences pour l'évaluation des procédures de mesure à l'aide de dispositifs de prélèvement par pompage (ISO 22065:2020)

Le présent document spécifie les exigences de performance et les méthodes d'essai, dans des conditions de laboratoires spécifiées, pour l'évaluation des dispositifs de prélèvement par pompage employés conjointement avec une pompe de prélèvement d'air, ainsi que pour l'évaluation des procédures utilisant ces dispositifs de prélèvement pour la détermination des gaz et des vapeurs dans les atmosphères des lieux de travail.
Le présent document traite des exigences à destination des développeurs de méthodes et/ou des fabricants.
NOTE 1    Pour les besoins du présent document, un fabricant peut être toute entité commerciale ou non commerciale.
NOTE 2    En ce qui concerne le prélèvement de composés semi-volatils pouvant exister sous forme de mélange de vapeurs et de particules aériennes au sein des atmosphères des lieux de travail, voir l'EN 13936.
Le présent document est applicable aux dispositifs de prélèvement par pompage et aux procédures de mesure utilisant ces dispositifs de prélèvement pour lesquels le prélèvement et l'analyse sont effectués dans des étapes séparées.
Le présent document n'est pas applicable:
—     aux dispositifs de prélèvement par pompage utilisés pour la détermination directe des concentrations, par exemple les tubes détecteurs à plage colorée;
—     aux dispositifs de prélèvement basés sur l'adsorption dans un liquide suivie de l'analyse de la solution (barboteurs).

Zrak na delovnem mestu - Plini in pare - Zahteve za vrednotenje postopkov za merjenje z vzorčevalniki s črpanjem (ISO 22065:2020)

General Information

• Status: Published
• Publication Date: 24-Nov-2020
• Withdrawal Date: 30-May-2021
• ICS: 13.040.30 - Workplace atmospheres
• Technical Committee: CEN/TC 137 - Assessment of workplace exposure
• Drafting Committee: CEN/TC 137 - Assessment of workplace exposure
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 25-Nov-2020
• Completion Date: 25-Nov-2020
• Ref Project: SIST EN ISO 22065:2021 - Workplace air - Gases and vapours - Requirements for evaluation of measuring procedures using pumped samplers (ISO 22065:2020)

Relations

• Replaces: EN ISO 22065:2019 - Workplace air - Gases and vapours - Requirements for evaluation of measuring procedures using pumped samplers (ISO 22065:2019)
• Effective Date: 02-Dec-2020

Overview

EN ISO 22065:2020 - Workplace air - Gases and vapours - Requirements for evaluation of measuring procedures using pumped samplers - defines performance requirements and laboratory test methods for assessing pumped samplers (used with an air sampling pump) and the associated measuring procedures for gases and vapours in workplace atmospheres. It applies where sampling and analysis are carried out in separate stages and is intended for method developers and manufacturers (commercial or non‑commercial).

Key topics and requirements

• Scope and applicability
  • Applies to pumped samplers and procedures where sampling and analysis are separate.
  • Exclusions: direct-read detector tubes (length-of-stain) and bubblers (liquid sorption).
  • Cross-reference: sampling of semi-volatile compounds → EN 13936.
• Sampler requirements
  • Flow resistance characterization.
  • Leak testing for relevant sampler types.
  • Shelf life and stability of impregnated supports.
  • Identification, marking and instructions for use supplied by manufacturers.
• Measuring procedure requirements
  • Defined sampling procedures (recommended conditions and breakthrough assessments).
  • Analytical procedure requirements and performance evaluation.
  • Method recovery, precision and estimation of expanded uncertainty.
  • Comprehensive method description and test report content requirements.
• Test methods and general conditions
  • Prescribed laboratory conditions, reagents and apparatus.
  • Generation and determination of calibration gas mixtures and independent verification methods.
  • Test methods for determination of recommended sampling conditions, method validation experiments, and uncertainty estimation.
• Informative annexes
  • Examples: breakthrough volume, method validation experiments, uncertainty estimation and worked examples of expanded uncertainty.

Applications and users

EN ISO 22065:2020 is practical for:

• Manufacturers of pumped samplers and sorbent media validating product performance.
• Method developers and occupational hygiene laboratories validating sampling and analytical procedures for workplace exposure assessment.
• Health & safety professionals and industrial hygienists specifying validated sampling methods for regulatory compliance and risk assessment.
• Test laboratories preparing method validation reports and uncertainty budgets.

Related standards

• ISO 20581 - general requirements for performance of procedures for measuring chemical agents in workplace atmospheres (framework referenced).
• EN 13936 - sampling of semi-volatile compounds (vapour/particle mixtures).

Keywords: EN ISO 22065:2020, workplace air, pumped samplers, gases and vapours, method validation, sampling procedures, occupational exposure, measurement uncertainty.

Standards Content (Sample)

SLOVENSKI STANDARD
01-januar-2021
Nadomešča:
SIST EN ISO 22065:2019
Zrak na delovnem mestu - Plini in pare - Zahteve za vrednotenje postopkov za
merjenje z vzorčevalniki s črpanjem (ISO 22065:2020)
Workplace air - Gases and vapours - Requirements for evaluation of measuring
procedures using pumped samplers (ISO 22065:2020)
Arbeitsplatzatmosphäre - Gase und Dämpfe - Anforderungen und Prüfverfahren zur
Messung mit pumpenbetriebenen Probenahmeeinrichtungen (ISO 22065:2020)
Air des lieux de travail - Gaz et vapeurs - Exigences pour l'évaluation des procédures de
mesure à l'aide de dispositifs de prélèvement par pompage (ISO 22065:2020)
Ta slovenski standard je istoveten z: EN ISO 22065:2020
ICS:
13.040.30 Kakovost zraka na delovnem Workplace atmospheres
mestu
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 22065
EUROPEAN STANDARD
NORME EUROPÉENNE
November 2020
EUROPÄISCHE NORM
ICS 13.040.30 Supersedes EN ISO 22065:2019
English Version
Workplace air - Gases and vapours - Requirements for
evaluation of measuring procedures using pumped
samplers (ISO 22065:2020)
Air des lieux de travail - Gaz et vapeurs - Exigences Arbeitsplatzatmosphäre - Gase und Dämpfe -
pour l'évaluation des procédures de mesure à l'aide de Anforderungen und Prüfverfahren zur Messung mit
dispositifs de prélèvement par pompage (ISO pumpenbetriebenen Probenahmeeinrichtungen (ISO
22065:2020) 22065:2020)
This European Standard was approved by CEN on 17 October 2020.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2020 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 22065:2020 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
This document (EN ISO 22065:2020) has been prepared by Technical Committee ISO/TC 146 "Air
quality" in collaboration with Technical Committee CEN/TC 137 “Assessment of workplace exposure to
chemical and biological agents” the secretariat of which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by May 2021, and conflicting national standards shall be
withdrawn at the latest by May 2021.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 22065:2019.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the
United Kingdom.
Endorsement notice
The text of ISO 22065:2020 has been approved by CEN as EN ISO 22065:2020 without any modification.

INTERNATIONAL ISO
STANDARD 22065
Second edition
2020-11
Workplace air — Gases and vapours
— Requirements for evaluation of
measuring procedures using pumped
samplers
Air des lieux de travail — Gaz et vapeurs — Exigences pour
l'évaluation des procédures de mesure à l'aide de dispositifs de
prélèvement par pompage
Reference number
ISO 22065:2020(E)
©
ISO 2020
ISO 22065:2020(E)
© ISO 2020
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting
on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address
below or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: [phone]
Email: [e-mail]
Website: www.iso.org
Published in Switzerland
ii © ISO 2020 – All rights reserved

ISO 22065:2020(E)
Contents Page
Foreword .v
Introduction .vi
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Symbols and abbreviated terms . 2
5 Sampler types . 3
6 Requirements . 3
6.1 General . 3
6.2 Sampler requirements . 3
6.2.1 Flow resistance . 3
6.2.2 Sampler leak test (for Type B samplers) . 4
6.2.3 Shelf life . 5
6.2.4 Sampler identification . 5
6.2.5 Marking . 5
6.2.6 Instructions for use. 5
6.3 Measuring procedure requirements . 5
6.3.1 Sampling procedure requirements . 5
6.3.2 Analytical procedure requirements . 6
6.3.3 Expanded uncertainty . 7
6.3.4 Method description . 7
7 General test conditions . 8
7.1 Reagents. 8
7.2 Apparatus . 8
7.3 Calibration gas mixture . 8
7.3.1 Generation . 8
7.3.2 Determination of mass concentration . 9
7.3.3 Independent method . 9
8 Test methods . 9
8.1 General . 9
8.2 Sampler test methods .10
8.2.1 Flow resistance .10
8.2.2 Sampler leak test (for Type B samplers) .10
8.2.3 Shelf life (for Type A impregnated supports) .10
8.2.4 Sampler identification .11
8.2.5 Marking .11
8.2.6 Instructions for use.11
8.3 Measuring procedure test methods .11
8.3.1 Determination of the recommended sampling conditions .11
8.3.2 Analytical procedure test methods .14
8.3.3 Method recovery and method precision .15
8.4 Uncertainty of measurement .17
8.4.1 Identification of random and non-random uncertainty components .17
8.4.2 Estimation of individual uncertainty components .17
8.4.3 Calculation of expanded uncertainty .19
9 Test report .19
Annex A (informative) Examples for the determination of the breakthrough volume .20
Annex B (informative) Experiments for method validation .22
Annex C (informative) Estimation of uncertainty of measurement .24
ISO 22065:2020(E)
Annex D (informative) Example for estimation of expanded uncertainty .34
Bibliography .37
iv © ISO 2020 – All rights reserved

ISO 22065:2020(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/ directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www .iso .org/ patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and
expressions related to conformity assessment, as well as information about ISO's adherence to the
World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT) see www .iso .org/
iso/ foreword .html.
This document was prepared by Technical Committee ISO/TC 146, Air quality, Subcommittee SC 2,
Workplace atmospheres.
This second edition cancels and replaces the first edition (ISO 22065:2019), of which it constitutes a
minor revision. The changes compared to the previous edition are as follows:
— Editorial updates.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www .iso .org/ members .html.
ISO 22065:2020(E)
Introduction
This document provides a framework for assessing the performance of procedures for measuring
gases and vapours against the general requirements for the performance of procedures for measuring
chemical agents in workplace atmospheres as specified in ISO 20581. It enables manufacturers and
users of pumped samplers and developers and users of procedures for measuring gases and vapours to
adopt a consistent approach to method validation (see Annex B).
vi © ISO 2020 – All rights reserved

INTERNATIONAL STANDARD ISO 22065:2020(E)
Workplace air — Gases and vapours — Requirements
for evaluation of measuring procedures using pumped
samplers
1 Scope
This document specifies performance requirements and test methods under prescribed laboratory
conditions for the evaluation of pumped samplers used in conjunction with an air sampling pump and of
procedures using these samplers for the determination of gases and vapours in workplace atmospheres.
This document addresses requirements for method developers and/or manufacturers.
NOTE 1 For the purposes of this document, a manufacturer can be any commercial or non-commercial entity.
NOTE 2 For the sampling of semi-volatile compounds which can appear as a mixture of vapours and airborne
particles in workplace atmospheres see EN 13936.
This document is applicable to pumped samplers and measuring procedures using these samplers in
which sampling and analysis are carried out in separate stages.
This document is not applicable to:
— pumped samplers which are used for the direct determination of concentrations, for example,
length-of-stain detector tubes;
— samplers which rely on sorption into a liquid, and subsequent analysis of the solution (bubblers).
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
ISO 8655-2, Piston-operated volumetric apparatus — Part 2: Piston pipettes
ISO 8655-6, Piston-operated volumetric apparatus — Part 6: Gravimetric methods for the determination
of measurement error
ISO 13137:2013, Workplace atmospheres — Pumps for personal sampling of chemical and biological
agents — Requirements and test methods
ISO 18158, Workplace air — Terminology
ISO 20581, Workplace air — General requirements for the performance of procedures for the measurement
of chemical agents
3 Terms and definitions
For the purposes of this document, the terms and definitions given in ISO 18158 apply.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at https:// www .iso .org/ obp
— IEC Electropedia: available at http:// www .electropedia .org/
ISO 22065:2020(E)
4 Symbols and abbreviated terms
For the purposes of this document, the following symbols and abbreviations apply.
NOTE See 8.4 and Annex C for symbols used in conjunction with uncertainty of measurement only.
CRM certified reference material
LV limit value
m mass of analyte desorbed from tube blank, in micrograms (µg)
a1
m mass of analyte desorbed from spiked tube, in micrograms (µg)
a2
m maximum mass uptake of analyte in a leak test performed on a sealed sampler used for
a,lt
making measurements for comparison with a long-term limit value, in milligrams (mg)
m maximum mass uptake of analyte in a leak test performed on a sealed sampler used for
a,st
making measurements for comparison with a short-term limit value, in milligrams (mg)
−1

m mass loss from permeation tube, in micrograms per minute (µg ∙ min )
−1
M molar mass of analyte, in grams per mole (g ∙ mol )
a
n number of replicate samples
p pressure of the test atmosphere sampled, in kilopascals (kPa)
at
R method recovery
me
R analytical recovery
an
RH relative humidity of the test atmosphere sampled, in percent (%)
t hold-up time of the unretained substance, in minutes (min)
H
t sampling time, in minutes (min)
s
T temperature of the test atmosphere sampled, in Kelvins (K)
at
V volume of the test atmosphere sampled, in litres (l)
at
V gas (vapour) hold-up volume (dead volume), in litres (l)
H
V uncorrected retention volume, in litres (l)
R
(V )′ corrected retention volume, in litres (l)
R
−1
v
flow rate into the exposure chamber, for example, in litres per minute (l ∙ min )
−1
v volumetric air flow rate through the sampler, for example, in litres per minute (l ∙ min )
a
β mass concentration of the analyte in the calibration gas mixture, in milligrams per cubic
a
−3
metre (mg ∙ m )
mean mass concentration of the analyte recovered from the test gas atmosphere, in mil-
β
a,R
−3
ligrams per cubic metre (mg ∙ m )
2 © ISO 2020 – All rights reserved

ISO 22065:2020(E)
β mass concentration of the calibration gas mixture, in milligrams per cubic metre
cg
−3
(mg ∙ m )
ϑ temperature of the test atmosphere sampled, in degrees Celsius (°C)
at
K coefficient of variation (CV)
v
NOTE  The predecessor term "relative standard deviation" is deprecated. See also
ISO 3534-1:2006, 2.38, Note 2.
−3
ρ long-term limit value given as concentration, in milligrams per cubic metre (mg ∙ m )
LV,lt
−3
ρ short-term limit value given as concentration, in milligrams per cubic metre (mg ∙ m )
LV,st
−1
ϕ volume fraction of the analyte, in microlitres per litre (µl ∙ l )
a
5 Sampler types
Samplers for gases and vapours can be divided into type A samplers and type B samplers:
— Type A samplers rely on sorption onto a collection substrate which can be impregnated with a
reagent. The collection substrate is always desorbed with a solvent, and subsequently analysed.
— Type B samplers rely on sorption onto a solid, thermal desorption, and analysis of the desorbate.
6 Requirements
6.1 General
Some requirements (see 6.2) shall be initially verified by the manufacturer once for each type of
sampler. Other requirements (see 6.3) shall be verified for each combination sampler/chemical agent.
Measuring procedures shall meet the requirements for measuring procedures specified in 6.3. When
use of a sampler for measurement of a particular gas or vapour is claimed, the sampler shall meet the
requirements specified in 6.2.
Known or suspected interferences shall be noted as required in 6.3.4.1. The results of any tests
performed to evaluate interferences, including suitable and sufficient information to minimize their
effects shall be presented in the method report as required in 6.3.4.2.
NOTE No useful performance requirements can be given for the effect of interferents (with the exception
of water vapour). The effect of interferents is difficult to predict for a non-ideal sorbent without adsorption
isotherm data on mixed systems which is normally unavailable.
6.2 Sampler requirements
6.2.1 Flow resistance
When tested in accordance with 8.2.1, at least 95 % of samplers shall have a back pressure less than the
appropriate maximum value indicated in Table 1. A minimum of 20 samplers shall be tested.
NOTE Typical back pressure values for type A samplers and type B samplers are given in [1].
ISO 22065:2020(E)
Table 1 — Maximum back pressures
Maximum back
Sampler type pressure
kPa
Type A (solvent desorption) ≤10
Type B (thermal desorption) ≤3,5
6.2.2 Sampler leak test (for Type B samplers)
When tested in accordance with 8.2.2, for substances with a long-term limit value the maximum
leakage, i.e. the maximum mass uptake of analyte above the blank value (see 6.3.2.3), shall be less than
m calculated according to Formula (1), in milligrams (mg), as follows:
a,lt
−3
m =×01,,ρ 240××00110 (1)
()
a,lt LV,lt
where
m is the maximum mass uptake of analyte in a leak test performed on a sealed sampler used
a,lt
for making measurements for comparison with a long-term limit value;
ρ is the long-term limit value of the substance given as concentration, in milligrams per cubic
LV,lt
−3
metre (mg ∙ m );
240 is the reference period, in minutes (min);
−1
0,01 is the nominal minimum flow rate for type B samplers, in litres per minute (l ∙ min );
−3
10 is a factor applied to convert the nominal minimum flow rate from litres per minute (l/min)
3 −1
to cubic metres per minute (m ∙ min );
1/3 is a factor applied to calculate the maximum permitted leakage.
When tested in accordance with 8.2.2, for substances with a short-term limit value the maximum
leakage, i.e. the maximum mass uptake of analyte above the blank value (see 6.3.2.3), shall be less than
m calculated according to Formula (2), in milligrams (mg), as follows:
a,st
−3
m =×05,,ρ 15××00110 (2)
()
a,st LV,st
where
m is the maximum mass uptake of analyte in a leak test performed on a sealed sampler used
a,st
for making measurements for comparison with a short-term limit value;
ρ is the short-term limit value of the substance given as concentration, in milligrams per cubic
LV,st
−3
metre (mg ∙ m );
15 is the reference period, in minutes (min);
−1
0,01 is the nominal minimum flow rate for type B samplers, in litres per minute (l ∙ min );
−3
10 is a factor applied to convert the nominal minimum flow rate from litres per minute
−1 3 −1
(l ∙ min ) to cubic metres per minute (m ∙ min );
1/3 is a factor applied to calculate the maximum permitted leakage.
4 © ISO 2020 – All rights reserved

ISO 22065:2020(E)
6.2.3 Shelf life
The manufacturer shall specify the shelf life of the sampler when stored in its original package. During
this period the sampler shall fulfil all requirements.
6.2.4 Sampler identification
Samplers shall be uniquely identified.
6.2.5 Marking
Samplers shall be marked with at least the following:
a) manufacturer’s name,
b) product identification including batch identification, where available,
c) indication of the direction of air flow,
d) shelf life (or expiry date), and
e) number of this document.
NOTE The marking with the number of this document implies only that the sampler fulfils the requirements
given in 6.2.
If required due to limited space, the marking may be placed on the packaging of the sampler. However,
at least the product identification, batch identification, where available, and direction of air flow shall
be indicated on the sampler.
6.2.6 Instructions for use
The instructions for use supplied with the sampler shall be written in the principal language(s) used in
the countries where the sampler is to be marketed. They shall contain at least the following information:
a) designated use (general purpose for a number of gases and vapours or, specific, for a particular gas
or vapour, see 6.1),
b) assurance that blank value meets specifications, where necessary for a particular gas or vapour,
(where a blank value is important),
c) directions for proper handling of the sampler, including opening and closing,
d) general information on the principle of use, for example, sorbent type, reaction of the reagent
impregnated solid, desorption method,
e) information on the range of temperature where the sampler can be used,
f) information on storage and transport, and
g) information on health or environmental hazards and method of disposal.
6.3 Measuring procedure requirements
6.3.1 Sampling procedure requirements
6.3.1.1 General
Sampling conditions (sample volume, flow rate and sampling time) shall be established according to
the LV assigned to the compounds of interest, for example, short-term limit value, long-term limit value
or both.
ISO 22065:2020(E)
6.3.1.2 Sample volume
The recommended sample volume which is calculated from the recommended flow rate and the
reference period should conform to the sampler capacity verification test (see 8.3.1.2).
6.3.1.3 Air flow rate
6.3.1.3.1 Determination of the maximum air flow rate (only for impregnated filters)
When tested according to 8.3.1.4, the maximum air flow rate shall be 90 % of the flow rate at which the
breakthrough volume drops by 5 %.
6.3.1.3.2 Determination of the minimum air flow rate (only for thermal desorption)
A minimum air flow rate shall be established according to the test given in 8.3.1.5.
6.3.1.4 Storage conditions after sampling
The storage conditions after sampling shall be specified. When tested in accordance with 8.3.1.6, the
mean value of the method recovery after storage shall not differ by more than 10 % from the value
before storage.
6.3.2 Analytical procedure requirements
6.3.2.1 Limit of quantification
The limit of quantification shall be lower than or equal to the calculated mass of analyte that would be
collected for the minimum air sample volume specified in the measuring procedure at the following
concentrations:
— 0,1 LV for substances with long-term limit value,
— 0,5 LV for substances with short-term limit value only.
6.3.2.2 Analytical recovery
When tested in accordance with 8.3.2.2, the analytical recovery R shall be
an
— for Type A samplers, R ≥ 75 % with K ≤ 10 % at each loading, and
an v
— for Type B samplers, R ≥ 95 % with K ≤ 10 % at each loading.
an v
The values given for analytical recovery are targets; lower values may be used provided equivalent
precision is achieved.
6.3.2.3 Blank value
In order to obtain acceptable values for the limit of quantification of the method, the blank value of the
sampling media should be as low as technically possible.
When tested in accordance with 8.3.2.3 the blank value shall be less than one-tenth of the calculated
mass collected by the sampler during the recommended sampling time at the recommended air flow
rate and at concentrations of
— 0,1 LV for substances with long-term limit value, and
— 0,5 LV for substances with short-term limit value only.
NOTE Higher blank values can be allowed provided the requirement of 6.3.2.1 is met.
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ISO 22065:2020(E)
Where it is known that the blank value is significant and varies between batches of samplers, it shall be
checked regularly.
In order to eliminate any contamination which could occur during storage before use, Type B samplers
should be cleaned by taking them through the thermal desorption procedure. This cleaning process
should be carried out as close as possible to the time when the samplers will be used.
6.3.3 Expanded uncertainty
When tested in accordance with 8.3 the expanded uncertainty, calculated in accordance with 8.4, shall
meet the requirements given in ISO 20581.
The expanded uncertainty requirement shall be met from 10 °C to 40 °C and at relative humidities from
20 % to 80 %.
6.3.4 Method description
6.3.4.1 Scope of the measuring procedure
The scope of the measuring procedure shall give information about the following:
a) principle of the method;
b) chemical agents covered by the measuring procedure;
c) analytical technique used;
d) working ranges;
e) chemical agents for which the measuring procedure is known to be adequate but not completely
validated according to this document, especially in case of compounds of the same chemical family
or homologous series;
f) chemical agents for which the measuring procedure is known to be inadequate;
g) any known interferences.
6.3.4.2 Method performance
The measuring procedure shall give information about method performance, including the following:
a) the chemical agents for which measuring procedure has been shown to be effective;
b) the range of concentrations of chemical agents in air, sample volume and range of environmental
conditions over which the measuring procedure has been shown to meet the performance criteria
for expanded uncertainty prescribed in ISO 20581;
c) the limit of quantification of the measuring procedure for chemical agents of interest;
d) full details of any known interferences, including suitable and sufficient information on how to
minimise their effects.
6.3.4.3 Apparatus and reagents
The measuring procedure shall
a) specify that the sampler used conforms to the provisions of this document;
b) contain a requirement that the sampling pumps used conform to the provisions given in ISO 13137
and specify any characteristics of the sampling pumps additionally required;
ISO 22065:2020(E)
c) define the required characteristics of the apparatus and test equipment to be used;
d) specify the quality of the reagents to be used.
6.3.4.4 Safety information
The measuring procedure shall provide suitable and sufficient information on the safety hazards
associated with the reagents and equipment used in the procedure.
7 General test conditions
7.1 Reagents
Wherever possible, only reagents of analytical grade may be used.
7.2 Apparatus
7.2.1 Test equipment as stated in 7.2.2 to 7.2.7 shall be used.
7.2.2 Usual laboratory apparatus and resources.
7.2.3 A dynamic system for generating, pre-mixing and delivering a known concentration of a
test gas or vapour in air (see ISO 6145-1, ISO 6145-4 and ISO 6145-6), including at least:
— an exposure chamber constructed of inert material such as glass or polytetrafluorethylene (PTFE),
through which the generated test atmosphere is passed, of sufficient capacity to accommodate
simultaneously at least six test samplers and six samplers of one independent method (see 7.3.3)
positioned in such a manner that there is no interference between each sampler;
— provisions for measuring, controlling and varying the air flow rate through the chamber and the
concentration, temperature and relative humidity of the calibration gas mixture.
NOTE 1 It is also possible to use a smaller exposure chamber and to carry out repeat experiments to obtain at
least six pairs of data.
NOTE 2 Where test gas or vapour atmospheres cannot be generated because of stability or safety
consideration, alternative procedures for spiking samples can be considered.
7.2.4 Appropriate sampling pumps which meet the performance requirements of ISO 13137.
−1 −1
7.2.5 Flowmeter(s), calibrated, suitable over range(s) which include 10 ml ∙ min to 2 000 ml ∙ min ,
with a measurement uncertainty less than ±2 %.
7.2.6 Micropipettes or syringes, for applying known volumes of standard solutions, in conformance
with the requirements of ISO 8655-2 and with a calibration checked in accordance with ISO 8655-6.
7.2.7 Instruments for analysing the gas, vapour or a characteristic reaction product collected by
either the test sampler or an independent sampling procedure.
7.3 Calibration gas mixture
7.3.1 Generation
Set up a calibration gas mixture at the concentration and values of temperature, relative humidity, etc.
specified in the appropriate test methods in Clause 8.
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ISO 22065:2020(E)
Ensure that the flow rate into the exposure chamber exceeds the combined sampling rate of all samplers
by at least 25 %.
7.3.2 Determination of mass concentration
Calculate the mass concentration of the calibration gas mixture, β , given in milligrams per cubic metre
cg
−3
(mg ∙ m ), from the test atmosphere generation parameters.
For example, for a permeation cell system, the delivered mass concentration is calculated by Formula (3)
as follows:

m
β = (3)
cg

v
where
−1
m is the mass loss from permeation tube, in micrograms per minute (µg ∙ min ); and
−1

v
is the flow rate into the exposure chamber, for example, in litres per minute (l ∙ min ).
NOTE 1 The example does not give a preference for permeation systems for generating calibration gas
mixtures.
Determine the mean mass concentration of the test atmosphere within the exposure chamber
experimentally using the results of the independent method described in 7.3.3. A correction may be
applied for any known bias in the independent method.
Compare the determined mean mass concentration with the calculated value. If the experimentally
determined value is within ±10 % of the calculated value of the mass concentration of the delivered
test atmosphere, take the calculated value as the true value of the delivered mass concentration. If this
requirement is not met, then adjustments shall be made or an alternative generation procedure shall be
used or the independent method shall be verified.
If it is not possible to calculate a mass concentration of the calibration gas mixture, for example, for
reactive gases, the value determined by the independent method shall be used as the true value.
NOTE 2 There can be situations, where it is preferable to use the experimentally determined mass
concentration rather than the calculated mass concentration.
7.3.3 Independent method
The mass concentration of the generated calibration gas mixture in the exposure chamber shall be
verified as follows:
a) by an independent method, which has been validated using an established protocol, for example, a
diffusive sampler method, bubbler method or a different sorbent tube method; or
b) by using an independently calibrated on-line instrument, for example, a flame ionization detector
or an infrared spectrometer.
NOTE For performance requirements for diffusive samplers see EN 838.
8 Test methods
8.1 General
If it is known in advance that a certain type of sampler is unaffected by an environmental influence,
then the relevant tests in 8.3.3.2 to 8.3.3.4 may be modified to examine only the factors likely to have
an influence.
ISO 22065:2020(E)
There are two levels of evaluation specified as follows:
a) level 1: A measuring procedure evaluated for the analyte of interest in accordance with the
normative part of this document;
b) level 2: A measuring procedure deemed to be in conformance with the normative part of this
document on the basis that the analyte of interest is an analogue within a homologous series, both
upper and lower members of which have been tested and shown to conform with level 1.
NOTE Some special groups of substances (for example, toluene, xylenes) usually isomers, can be treated as
homologous when it is known that their chemical and physical properties are very similar.
If it is known in advance, or through testing, that a certain sampler-analyte combination is affected by
an environmental influence then the relevant tests in 8.3.3.2 to 8.3.3.4 may be modified to investigate
performance over a more restricted range of conditions.
8.2 Sampler test methods
8.2.1 Flow resistance
Assemble a sampling train consisting of a sampling pump (see 7.2.4), a representative sampler, a
differential pressure gauge, which is inserted via a T-piece between the sampling pump and the sampler,
and a flow meter in front of the sampler. All connections shall be leak tight, having connecting tubing as
short as possible and a minimum internal diameter of 6 mm.
Measure the back pressure relative to ambient pressure with the differential pressure gauge, carry out
the flow resistance test at the maximum recommended flow rate and compare with the required value
in Table 1 (see 6.2.1).
8.2.2 Sampler leak test (for Type B samplers)
Expose a set of six sealed samplers to a test atmosphere under the following exposure conditions:
— concentration: 2 LV;
— time: 4 h;
— relative humidity: (50 ± 5) %;
— temperature: (20 ± 2) °C;
Analyse the set to determine any leakage.
8.2.3 Shelf life (for Type A impregnated supports)
Store the sampler at the limits of the environmental conditions specified by the manufacturer and/or
in the measuring procedure. At the end of the specified shelf-life, test the sampler under the following
exposure conditions:
— concentration: 2 LV;
— time: recommended sampling time;
— flow rate: recommended flow rate;
— relative humidity: (80 ± 5) %;
— temperature: (40 ± 2) °C.
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ISO 22065:2020(E)
8.2.4 Sampler identification
Perform a visual check.
8.2.5 Marking
Perform a visual check.
8.2.6 Instructions for use
Perform a visual check.
8.3 Measuring procedure test methods
8.3.1 Determination of the recommended sampling conditions
8.3.1.1 Selection of sampler capacity test
Perform the sampler capacity verification test in 8.3.1.2 or the sampler breakthrough test in 8.3.1.3
taking into consideration whether the capacity of the sampler is likely to be high for the substance to
be measured. This will depend upon the characteristics of the substance and the sampler, for example,
nature and amount of the sampling substrate, and the limit value for the substance concerned.
8.3.1.2 Sampler capacity verification test
Sample from a test atmosphere (generated by using the apparatus described in 7.2.3) with a minimum
of three samplers under the following exposure conditions:
— concentration: 2 LV;
— time:
— For long-term LV: reference period plus a minimum of 1 h or the sampling period, if longer,
— For short-term LV: twice the reference period;
— flow rate: recommended maximum flow rate;
— relative humidity: (80 ± 5) %;
— temperature: (20 ± 2) °C.
For samplers without a back-up section use two samplers in series.
Analyse the samplers after the test. The amount of the test substance recovered in the back-up section
of sampler shall be less or equal than 5 % of the total amount recovered. If the amount recovered in the
back-up section of sampler is greater than 5 %, carry out the sampler breakthrough test specified in
8.3.1.3.
NOTE If the amount recovered in the back-up section of sampler (or second sampler in series) is greater than
5 %, the test can be repeated at sequentially lower flow-rates or reduced sampling period until the
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