Animal feeding stuffs - Isolation and enumeration of Bifidobacterium spp.

This European Standard defines general rules for the enumeration of probiotic bifidobacteria in feed samples (additives, premixtures and feeding stuffs) that contain bifidobacteria as a single bacterial component or in a mixture with other microorganisms. This standard is not applicable for mineral feeds which are defined as complementary feeding stuffs composed mainly of minerals and containing at least 40% crude ash (Council Directive 79/373/EEC) [3].
There are different categories of feed samples:
a)   Additives containing about 1010 colony forming units (CFU)/g
b)   Premixtures containing  about 108 CFU/g
c)   Feeds, meal or pellets, which contain about 106 CFU/g and include complete feeding stuffs, and milk replacers.
The detection limit is as defined in ISO 7218.

Futtermittel - Keimzählung von Bifidobacterium spp.

Diese Europäische Norm legt allgemeine Regeln für die Zählung von probiotischen Bifidobakterien in Futter-mittelproben (Zusatzstoffe, Vormischungen und Futtermittel) fest, die Bifidobakterien als einzelnen probiotischen Bestandteil oder in einem Gemisch mit anderen probiotischen Mikroorganismen enthalten. Diese Norm ist nicht anwendbar auf mineralische Futtermittel, die als Ergänzungsfuttermittel definiert sind, hauptsächlich aus Mineralien zusammengesetzt sind und mindestens 40 % Rohasche enthalten (Richtlinie 79/373/EWG) [3].
Es gibt unterschiedliche Kategorien von Futtermittelproben:
a) Zusatzstoffe, die etwa 1010 koloniebildende Einheiten (KbE/g) enthalten;
b) Vormischungen, die etwa 108 KbE/g enthalten;
c) Futtermittel, Mehl oder Pellets, die etwa 106 KbE/g enthalten, einschließlich Fertigfuttermittel und Milch-austauscher.
Die Nachweisgrenze entspricht der in EN ISO 7218 festgelegten.

Aliments des animaux - Isolement et dénombrement du Bifidobacterium spp.

La présente Norme européenne définit les règles générales de dénombrement des bifidobactéries probiotiques dans les échantillons d’aliments (additif, pré mélange et aliments des animaux) qui contiennent des bifidobactéries comme seul composant bactérien ou dans un mélange comportant d’autres micro organismes. La présente norme ne s’applique pas aux aliments minéraux définis comme aliments complémentaires constitués principalement de minéraux et contenant au moins 40 % de cendre brute (Directive 79/373/CEE du Conseil).
Il existe différentes catégories d’échantillons d’aliments :
   les additifs contenant environ 1010 UFC/g ;
   les prémélanges contenant environ 108 UFC/g ;
   les aliments, farines ou granulés qui contiennent environ 106 UFC/g et comprennent des aliments complets et des aliments d’allaitement.

Krma - Izolacija in štetje domnevno prisotnih Bifidobacterium spp

General Information

Status
Published
Publication Date
01-Sep-2009
Withdrawal Date
30-Mar-2010
Current Stage
6060 - Definitive text made available (DAV) - Publishing
Start Date
02-Sep-2009
Due Date
01-Nov-2009
Completion Date
02-Sep-2009

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2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Krma - Izolacija in štetje domnevno prisotnih Bifidobacterium sppFuttermittel - Keimzählung von Bifidobacterium sppAliments des animaux - Isolement et dénombrement du Bifidobacterium sppAnimal feeding stuffs - Isolation and enumeration of Bifidobacterium spp65.120KrmilaAnimal feeding stuffsICS:Ta slovenski standard je istoveten z:EN 15785:2009SIST EN 15785:2009en,fr,de01-december-2009SIST EN 15785:2009SLOVENSKI
STANDARD



SIST EN 15785:2009



EUROPEAN STANDARD NORME EUROPÉENNE EUROPÄISCHE NORM
EN 15785
September 2009 ICS 65.120 English Version
Animal feeding stuffs - Isolation and enumeration of Bifidobacterium spp.
Aliments des animaux - Isolement et dénombrement du Bifidobacterium spp.
Futtermittel - Keimzählung von Bifidobacterium spp. This European Standard was approved by CEN on 1 August 2009.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre:
Avenue Marnix 17,
B-1000 Brussels © 2009 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN 15785:2009: ESIST EN 15785:2009



EN 15785:2009 (E) 2 Contents Page Foreword .3 Introduction .4 1 Scope .5 2 Normative references .5 3 Terms and definitions .5 4 Principle .6 5 Diluent, media and phenotypic characterisation .6 6 Apparatus and glassware .8 7 Sampling .9 8 Preparation of test sample . 10 9 Procedure . 10 10 Expression of results . 12 11 Precision . 12 12 Test report . 13 Annex A (informative) Notes on procedures . 14 Annex B (informative) Results of the interlaboratory study . 15 Bibliography . 16
SIST EN 15785:2009



EN 15785:2009 (E) 3 Foreword This document (EN 15785:2009) has been prepared by Technical Committee CEN/TC 327 “Ani
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