CEN/TS 18044:2024

Ambient air - Determination of the concentration of levoglucosan - Chromatographic method

This document specifies a chromatographic method for the determination of levoglucosan in aqueous or organic extracts of filter samples collected in accordance with EN 12341:2023 [5]. The method has been tested for concentrations of ca. 10 ng/m3 up to ca. 3 000 ng/m3 with a sampling duration of 24 h. The procedure is also suitable for the determination of galactosan and mannosan.
Depending on the analysis instrumentation used, the carbohydrates inositol, glycerol, threitol/erythritol, xylitol, arabitol, sorbitol, mannitol, threalose, mannose, glucose, galactose and fructose can also be determined. However, no performance characteristics are given for these compounds in this document.

Außenluft - Bestimmung der Konzentration von Levoglucosan - Chromatographisches Verfahren

Dieses Dokument legt ein chromatographisches Verfahren zur Bestimmung von Levoglucosan in wässrigen oder organischen Extrakten von nach EN 12341:2023 [5] gesammelten Filterproben fest. Das Verfahren wurde für Konzentrationen von etwa 10 ng/m3 bis etwa 3 000 ng/m3 bei einer Probenahmedauer von 24 h geprüft. Das Verfahren ist auch zur Bestimmung von Galactosan und Mannosan geeignet.
Je nach verwendetem Analytikinstrumentarium können auch die Kohlenhydrate Inositol, Glycerol, Threitol/Erythritol, Xylitol, Arabitol, Sorbitol, Mannitol, Threalose, Mannose, Glucose, Galactose und Fructose bestimmt werden. In diesem Dokument werden jedoch keine Leistungskenngrößen für diese Verbindungen angegeben.

Air ambiant - Détermination de la concentration de lévoglucosan - Méthode chromatographique

Le présent document spécifie une méthode chromatographique pour la détermination du lévoglucosan dans des extraits aqueux ou organiques d’échantillons de filtres collectés conformément à l’EN 12341:2023 [5]. La méthode a été soumise à essai pour des concentrations d’environ 10 ng/m3 jusqu’à environ 3 000 ng/m3 avec une durée de prélèvement de 24 h. Le mode opératoire convient également pour la détermination du galactosan et du mannosan.
Selon les instruments d’analyse utilisés, il est également possible de déterminer les hydrates de carbone inositol, glycérol, thréitol/érythritol, xylitol, arabitol, sorbitol, mannitol, tréhalose, mannose, glucose, galactose et fructose. Cependant, le présent document ne spécifie aucune caractéristique de performance pour ces composés.

Zunanji zrak - Določanje koncentracije levoglukozana - Kromatografska metoda

General Information

• Status: Published
• Publication Date: 02-Jul-2024
• ICS: 13.040.20 - Ambient atmospheres
• Technical Committee: CEN/TC 264 - Air quality
• Drafting Committee: CEN/TC 264/WG 21 - Measurement method for B(a)P in Ambient Air
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 03-Jul-2024
• Due Date: 03-Dec-2024
• Completion Date: 03-Jul-2024
• Ref Project: SIST-TS CEN/TS 18044:2024 - Ambient air - Determination of the concentration of levoglucosan - Chromatographic method

Standards Content (Sample)

SLOVENSKI STANDARD
01-september-2024
Zunanji zrak - Določanje koncentracije levoglukozana - Kromatografska metoda
Ambient air - Determination of the concentration of levoglucosan - Chromatographic
method
Außenluft - Bestimmung der Konzentration von Levoglucosan - Chromatographisches
Verfahren
Air ambiant - Détermination de la concentration de lévoglucosan - Méthode
chromatographique
Ta slovenski standard je istoveten z: CEN/TS 18044:2024
ICS:
13.040.20 Kakovost okoljskega zraka Ambient atmospheres
71.040.50 Fizikalnokemijske analitske Physicochemical methods of
metode analysis
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

CEN/TS 18044
TECHNICAL SPECIFICATION
SPÉCIFICATION TECHNIQUE
July 2024
TECHNISCHE SPEZIFIKATION
ICS 13.040.20
English Version
Ambient air - Determination of the concentration of
levoglucosan - Chromatographic method
Air ambiant - Détermination de la concentration de Außenluft - Bestimmung der Konzentration von
lévoglucosan - Méthode chromatographique Levoglucosan - Chromatographisches Verfahren
This Technical Specification (CEN/TS) was approved by CEN on 12 May 2024 for provisional application.

The period of validity of this CEN/TS is limited initially to three years. After two years the members of CEN will be requested to
submit their comments, particularly on the question whether the CEN/TS can be converted into a European Standard.

CEN members are required to announce the existence of this CEN/TS in the same way as for an EN and to make the CEN/TS
available promptly at national level in an appropriate form. It is permissible to keep conflicting national standards in force (in
parallel to the CEN/TS) until the final decision about the possible conversion of the CEN/TS into an EN is reached.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2024 CEN All rights of exploitation in any form and by any means reserved Ref. No. CEN/TS 18044:2024 E
worldwide for CEN national Members.

Contents Page
European foreword . 4
Introduction . 5
1 Scope . 6
2 Normative references . 6
3 Terms and definitions . 6
4 Symbols and abbreviations . 6
5 Principle . 7
6 Equipment . 7
6.1 Sampling . 7
6.1.1 Sampling device . 7
6.1.2 Particle filter . 7
6.2 Apparatus for sample preparation . 7
6.3 Analytical equipment . 7
6.3.1 IC-PAD . 7
6.3.2 GC-MS . 8
6.4 Chemicals and accessories . 8
7 Sampling . 9
8 Sample preparation and analysis . 9
8.1 General. 9
8.2 IC-PAD method . 9
8.2.1 Sample preparation . 9
8.2.2 Eluent preparation . 9
8.2.3 Analysis . 9
8.2.4 Calibration . 9
8.3 GC-MS method . 10
8.3.1 Sample preparation . 10
8.3.2 Analysis . 10
8.3.3 Calibration . 10
9 Calculation of results . 10
10 Measurement uncertainty . 11
10.1 General. 11
10.2 Evaluation according to ISO 5725-2 . 11
10.3 Evaluation of laboratory data according to ISO/IEC Guide 98-3 . 12
11 Limit of detection and limit of quantification . 13
12 Interferences . 14
12.1 General. 14
12.2 IC-PAD . 14
12.3 GC-MS . 15
13 Quality assurance and quality control . 15
Annex A (informative) Application examples of the IC-PAD method . 16
A.1 Example 1 . 16
A.1.1 Apparatus . 16
A.1.2 Operating parameters. 16
A.1.3 Chemicals and accessories . 17
A.1.4 Procedure . 18
A.2 Example 2 . 21
A.2.1 Apparatus . 21
A.2.2 Operating parameters. 22
A.2.3 Chemicals and accessories . 22
A.2.4 Procedure . 23
Annex B (informative) Application example of the GC-MS method . 28
B.1 Apparatus . 28
B.2 GC/MS operating parameters . 28
B.3 Chemicals . 28
B.4 Procedure . 29
B.4.1 Calibration standards . 29
B.4.2 Sample preparation . 30
Annex C (informative) Calculation of the measurement uncertainty using results of a
laboratory intercomparison . 32
C.1 General . 32
C.2 Calculations according to ISO 5725-2. 32
C.3 Calculation of uncertainty parameters according to ISO 13528 (Q/Hampel method)
................................................................................................................................................................... 33
C.4 Comparison of the two calculation methods . 34
Annex D (informative) Possible requirements for the uncertainty of single steps in work up
and analysis for an overall uncertainty of 40 % . 37
Annex E (informative) Calculation of the limit of detection from the calibration function . 39
Annex F (informative) Illustrative chromatograms . 40
Bibliography . 42

European foreword
This document (CEN/TS 18044:2024) has been prepared by Technical Committee CEN/TC 264 “Air
quality”, the secretariat of which is held by DIN.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
Any feedback and questions on this document should be directed to the users’ national standards body.
A complete listing of these bodies can be found on the CEN website.
According to the CEN/CENELEC Internal Regulations, the national standards organisations of the
following countries are bound to announce this Technical Specification: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the
United Kingdom.
Introduction
Emissions from the combustion of biomass (e.g. wood firing) contribute to particulate air pollution (PM ,
PM ). Under inversion weather conditions in winter, the contribution of biomass combustion to the
2,5
particulate load can increase up to 50 % of the PM average daily value. As levoglucosan originates by
pyrolysis of materials containing cell
...