EN 16007:2011

Animal feeding stuffs - Determination of Ochratoxin A in animal feed by immunoaffinity column clean-up and High Performance Liquid Chromatography with fluorescence detection

This European Standard specifies a method for the determination of Ochratoxin A (OTA) in cereal based animal feed using immunoaffinity for clean-up followed by liquid-chromatography with fluorescence detection.
NOTE   The validated mass fraction range was 39 µg/kg to 338 µg/kg OTA.

Futtermittel - Bestimmung von Ochratoxin A in Tierfutter durch Reinigung an einer Immunoaffinitätssäule und Hochleistungs-Flüssig-Chromatographie mit Fluoreszenzdetektion

Diese Norm legt ein Verfahren für die Bestimmung von Ochratoxin A (OTA) in Futtermitteln auf Getreidebasis durch Hochleistungs-Flüssig-Chromatographie (HPLC) mit Fluoreszenzdetektion nach Reinigung an Immunoaffinitätssäulen fest. ANMERKUNG Der validierte Konzentrationsbereich liegt zwischen 39 μg/kg bis 338 μg/kg OTA.

Aliments pour animaux - Dosage de l'ochratoxine A dans les aliments pour animaux par purification sur colonne d'immuno-affinité et chromatographie liquide à haute performance avec détection par fluorescence

La présente Norme européenne spécifie une méthode de dosage de l’ochratoxine A (OTA) dans les aliments
pour animaux à base de céréales en utilisant une purification sur colonne d'immuno-affinité suivie d’une
chromatographie liquide avec détection par fluorescence

Krma - Določevanje ohratoksina A v krmi z imunoafinitetnim kolonskim čiščenjem in s tekočinsko kromatografijo visoke ločljivosti s fluorescentno detekcijo

Ta evropski standard opredeljuje metodo za določevanje ohratoksina A (OTA) v krmi na osnovi žit, z uporabo imunoafinitetnega čiščenja, ki mu sledi tekočinska kromatografija s fluorescentno detekcijo.

General Information

• Status: Published
• Publication Date: 02-Aug-2011
• ICS: 65.120 - Animal feeding stuffs
• Technical Committee: CEN/TC 327 - Animal feeding stuffs - Methods of sampling and analysis
• Drafting Committee: CEN/TC 327/WG 1 - Minerals and Contaminants
• Current Stage: 9060 - Closure of 2 Year Review Enquiry - Review Enquiry
• Start Date: 02-Sep-2022
• Completion Date: 02-Sep-2022
• Directive: 70/373/EC - Council Directive 70/373/EEC of 20 July 1970 on the introduction of Community methods of sampling and analysis for the official control of feeding-stuffs
• Mandate: M/382 - Mandate for standardisation addressed to CEN in the field of Methods of analysis for animal Feedingstuffs
• Ref Project: SIST EN 16007:2011 - Animal feeding stuffs - Determination of Ochratoxin A in animal feed by immunoaffinity column clean-up and High Performance Liquid Chromatography with fluorescence detection

Standards Content (Sample)

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Futtermittel - Bestimmung von Ochratoxin A in Tierfutter mit Reinigung an einer Immunoaffinitätssäule und Hochleistungs-Flüssig-Chromatographie mit FluoreszenzdetektionAliments pour animaux - Dosage de l'ochratoxine A dans les aliments pour animaux par purification sur colonne d'immuno-affinité et chromatographie liquide à haute performance avec détection par fluorescenceAnimal feeding stuffs - Determination of Ochratoxin A in animal feed by immunoaffinity column clean-up and High Performance Liquid Chromatography with fluorescence detection65.120KrmilaAnimal feeding stuffsICS:Ta slovenski standard je istoveten z:EN 16007:2011SIST EN 16007:2011en,fr,de01-oktober-2011SIST EN 16007:2011SLOVENSKI
STANDARD



SIST EN 16007:2011



EUROPEAN STANDARD NORME EUROPÉENNE EUROPÄISCHE NORM
EN 16007
August 2011 ICS 65.120 English Version
Animal feeding stuffs - Determination of Ochratoxin A in animal feed by immunoaffinity column clean-up and High Performance Liquid Chromatography with fluorescence detection
Aliments pour animaux - Dosage de l'ochratoxine A dans les aliments pour animaux par purification sur colonne d'immuno-affinité et chromatographie liquide à haute performance avec détection par fluorescence
Futtermittel - Bestimmung von Ochratoxin A in Tierfutter durch Reinigung an einer Immunoaffinitätssäule und Hochleistungs-Flüssig-Chromatographie mit Fluoreszenzdetektion This European Standard was approved by CEN on 25 June 2011.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION EUROPÄISCHES KOMITEE FÜR NORMUNG
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Avenue Marnix 17,
B-1000 Brussels © 2011 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN 16007:2011: ESIST EN 16007:2011



EN 16007:2011 (E) 2 Contents Page Foreword .31Scope .42Normative references .43Principle .44Reagents and materials .45Apparatus .76Sample preparation .87Measurements . 108Determination of concentrations . 119Precision . 1110Test report . 12Annex A (informative)
Example of a chromatogram . 14Annex B (informative)
Results of the interlaboratory study . 15Bibliography .
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